THE GROWTH OF ORCHID (DENDROBIUM SP) IN VITRO GIVING WITH COCONUT WATER ON DIFFERENT MEDIUM.

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

PROCEEDING
First Annual International Seminar on
Trends in Science and Science Education 2014

5th – 6th December 2014
Garuda Flaza Hotel - Medan

Editors:
Prof. Dr. Herbert Sipahutar, M.Sc.
Prof. Drs. Motlan, M.Sc, Ph.D.
Prof. Drs. Manihar Situmorang, M.Sc., Ph.D.
Prof. Dr. Muktar, M.Pd. Alkhafi
Maas Siregar, S.Si., M.Si. Drs.
Zulkifli Simatupang, M.Pd

Fakultas Matematika dan Ilmu Pengetahuan Alam

Universitas Negeri Medan
2015
Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

FROM THE EDITORS

The First International Seminar on Sciences and Science Education, ISOSE, organized
by Faculty of Mathematics and Natural Science of State University of Medan, was held on 4 – 5
December 2014 in Medan, North Sumatera, Indonesia. The seminar particularly encouraged
the interaction of research students and developing academics with the more established
academic community in an informal setting to present and to discuss new and current work. The
high quality of the papers and the discussion represent the thinking and experience of experts
and practitioners, researchres, lecturers and students in their particular fields and interests. The
papers contributed the most recent scientific knowledge known in science and science

education.
This proceeding contains all the paper presentated in the seminar, consisted of 11 papers
of Biological Sciences, 11 papers of Chemical Sciences, 3 papers of Mathematical Sciences, 14
papers of Physical Sciences and 39 papers of Science Education.
In addition to the contributed papers, an outstanding keynote presentation on National
Curriculum 2013 was made by Prof. Dr. Syawal Gultom (formerly Rector of State University of
Medan, Unimed), now as Head of Badan Pengembangan Sumberdaya Manusia Pendidikan of
Department of Education and Culture of Republic of Indonesia. This presentation gives all
pratisipants a new and comprehensive perspective on the orientation of national education in
the next era.
Two invited keynote presentations were given by Prof. Dr. Yaya Rukayadi from
Department of Food Science, Faculty of Food Science and Technology and Laboratory of
Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor
DarulEhsan, Malaysia who spoke on how to appreciate the nation through research javanese
turmeric or temulawak (Curcuma xanthorrhiza ROXB.), and by Dr. Phattrawan Tongkumchum,
Department of Mathematics and Computer Science, Faculty of Science and Technology, Prince
of Songkla University, Pattani, Thailand who spoke about the applications of the weighted sum
contrasts methods on graphing confidence interval for adjusted mean, their used for comparing
two and several groups, and adjustment for covariates.
We would like to express our deep appreciation to Prof. Dr. Ibnu Hajar, Rector of State

University of Medan for financial support by means of Dana DIPA Unimed FY 2014.We would
like to express our deep appreciation to Prof. Dr. Motlan (Dean of FMIPA Unimed), all sponsors,
all member of seminar committe, that make the seminar happen in a great succes.
We thank all authors and participants for their contributions.

Medan, February 2015
Editors

The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

FROM THE CHAIRMAN OF THE ISOSE 2014

Your Excellency, Bapak Prof. Ibnu Hajar Damanik, Rector of Universitas Negeri Medan (State
University of Medan)

Your Excellency, Bapak Prof. Khairil Ansari, Academic Affair Vice Rector of Universitas Negeri
Medan
Your Excellency, Bapak Chairul Azmi, Financial Affair Vice Rector of Universitas Negeri Medan
Your Excellency, Bapak Prof. Biner Ambarita, Students Affair Vice Rector of Universitas Negeri
Medan
Your Excellency, Bapak Prof. Berlin Sibarani, Cooperation Affair Vice Rector of Universitas
Negeri Medan
Your Excellency, Bapak Prof. Motlan, Dean of Fakultas Matematika dan Ilmu Pengetahuan
Alam, Universitas Negeri Medan
Your Excellency, Bapak and Ibu Deans of Faculties in Universitas Negeri Medan
Your Excellency, Bapak Prof. Drs. Manihar Situmorang, M.Sc., Ph.D., Head of Research
Institute of Universitas Negeri Medan
Your Excellency, Bapak Dr. Ridwan Abdullah Sani, M.Si., Head of LPM of Universitas Negeri
Medan.
Distinguished experts, researchers, lecturers, students, guests, and all participants, ladies and
gentlemen.
Good morning and welcome to Medan, North Sumatera.
Before I begin with a few words please understand that our keynote speaker, His excellency
Bapak Prof. Syawal Gultom, Head of Badan Pengembangan Sumberdaya Manusia Pendidikan
is on the way right now. Hopefully, this does not lessen the meaning of this seminar, because

he will be with us in the next several minutes and gives his talk in this room.
First, let us be thankful to the one and all-powerful God that on this fine morning we are still
given bodily and spiritual health and can gather together in this room, on our beloved capital city
of North Sumatra, Medan.
Next, to our invited speakers Prof. Yaya Rukayadi (from University Putra Malaysia), Prof.
Phattrawan Tongkumchum (from Prince Songkla University, Thailand), and Dr. Thomas Ouhel
Universitas Negeri Medan
The Character Building University

2

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

(from Prague University, Check) we also want to extend a warm and special welcome to this
seminar.
This seminar, International Seminar on Trends in Science and Science Education 2014 is
specially organized by Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri

Medan. The seminar is planned to be held regularly on annual based as an attempt of FMIPA
Unimed to prepare a good vehicle to our lecturer, researchers, students and all human
resources to communicate, exchange and disseminate their experiences at the international
level. The main objective of this seminar is to encourage collaboration among the different
actors in science and science education community so as to achieve a better result for the
benefit of the community. This first annual international seminar will be held from December 5 –
6, 2014.
It is expected that those who participate in the seminar will afterwards be familiar to be able to
interact with their international counterparts in their scientific area. To make this interaction
happen, the lecturer, researchers, and students should be able to improve the research quality
in their respective domains. Hopefully, by organizing the regular international seminar, will help
not only Fakultas Matematika dan Ilmu Pengetahuan Alam but also Universitas Negeri Medan
to realise its dream to a world class and character building university.
Approximately 300 participants from four different countries are attending this seminar. They
come from Chech Republic, Thailand, Malaysia, and of course from Indonesia. Most of the
participants are lecturers, researchers, teachers and students, so we hope that a deep mutual
interaction will unfold. The participants come from different backgrounds and institutions. Some
are from government institutions, some from research institutions and universities, and some
are from NGOs.
Approximately 100 seminar abstracts and full papers from science education, biology,

chemistry, physics, and mathematics sciences have been received by the seminar committe.
Most of the abstract have been edited and bound into an abstract collection book which is a part
of the seminar kit.The seminar full papers are now in editing stage by the committe before
publish in seminar proceeding that will be available in both printed and on-line forms, in the next
January 2015. Please, remind the committe if you want to get the copy of the seminar
proceeding.

The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

I would like to take this opportunity to acknowledge the important role of the honorable Prof. Ibu
Hajar, rector of Universitas Negeri Medan for giving us his full support and attention and for
providing his precious time to be with us and to honour us by opening this seminar.
Our sincere thanks also goes to the honorable Prof. Motlan, Dean of Fakultas Matematika dan
Ilmu Pengetahuan Alam, who have lead and encourage all the committe members to be always

focussed and worked hard even in a very short period of time to prepare the seminar.
I would also like to thank His Excellency Prof Syawal Gultom, former Rector of Universitas
Negeri Medan, and now Kepala Badan Pengembangan Sumber Daya Manusia Pendidikan,
Departemen Pendikan dan Kebudayaan Republik Indonesia, for being willing to share his
experiences, knowledge and skills with us.
My sincere thanks also goes to all members of the committe and to all staff of Fakultas
Matematika dan Ilmu Pengetahuan Alam for their continuous support and hard work because
without their assistance this seminar may not have taken place today.
As Newton stated in his second law of motion, “Every body continues in its state of rest, or of
uniform motion in a straight line, unless it is compelled to change that state by forces impressed
upon it”. It is time for us (as lecturers, researchers, students and all staffs of the faculty or
university ) to apply the necessary force to our specific daily role in order to make our motions
converge more closely. Where better to start than on science and education, which will form the
foundation of our development and character as the 21st century unfolds.
Finally, I conclude my speech by kindly inviting Honourable Prof. Ibnu Hajar Damanik, Rector of
Universitas Negeri Medan, to give special direction and officially open the seminar.
We wish you good luck and success in this endeavour.
Thank you for your attention.

Medan, December 5, 2014

Herbert Sipahutar
Chairman of the Seminar Committe

Universitas Negeri Medan
The Character Building University

4

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

TABLE OF CONTENT
FROM THE EDITORS
FROM THE CHAIRMAN OF ISOSE 2014
TABLE OF CONTENT

ii

iii
vi

Keynote Speaker
KS-001 Dasar Pertimbangan Penetapan Struktur Kurikulum 2013
Prof. Dr. Syawal Gultom, M.Pd.

1 - 10

Invited Speaker
IS-001

Confidence Intervals with Application to Environmental Studies in Southern
Thailand
Phattrawan Tongkumchum

IS-002

11 - 21

Appreciate the Nation Through Research Javanese Turmeric or Temulawak
(Curcuma xanthorrhiza ROXB.): Xanthorrhizol an “Angel” Compound in the
Rhizome of Temulawak and Its Applications
Yaya Rukayadi

22

Biological Science
BS-001

Isolation of Heat Shock Proteins Gene (HSPs-gene) in the Silkworm, Bombyx
mori (C301)
Masitta Tanjung, Maryani Cyccu Tobing, Syafruddin Ilyas, and Darma Bakti

BS-002

Mating Behavior of Male Mice After MSG Administration during Intra Uterine to
Mature Periods of Life
Herbert Sipahutar and Adriana Y.D. Lbn Gaol

BS-003

29 - 38

Ecobiological Review of Neolissochilus sumatranus (Ikan Batak) (Weber and de
Beaufort, 1916) In Asahan River, North Sumatera
Ternala Alexander Barus, Hesti Wahyuningsih, Eva Marlina Ginting, and Charles
PH Simanjuntak

BS-004

46 - 53

Diversity of Lichens on the Stands of Mahoni (Swieteniamacrophylla)
Functioning as Shade Plants in Medan
Ashar Hasairin, Nursahara Pasaribu, Lisdar I. Sudirman, and Retno Widhiastuti

BS-006

61 - 67

In Vitro Selection Uplandrice Nias Island to Aluminium Resistant Character and
Low pH through Somaclonal Variation and Gamma-Rays Irradiation
Syahmi Edi, Lazuardi and Idramsa

BS-008

68 - 77

Cassava Leaves Battery as Alternative Energy Based on Environment Friendly
Technology
M. Gade

BS-009

78 - 81

Description of Endophytic Fungi of Plants Raru (Cotylelobium melanoxylon )
Genus Alternaria
Uswatun Hasanah, Riwayati and Idramsa

BS-010

82 - 90

The Effect of Biji Mete (Scomberomorus commerson) in the Feed to the Decline
in Blood Cholesterol Level Hypercholesterolemic Male Mice (Mus musculus)
Rudi Kartika and Eddiyanto

vi

54 - 60

Studies on Species Diversity and Growth Rate of Mold in Musk Lime Pickle
(Citrofortunella microcarpa) Food Society of Melayu
Mhd. Yusuf Nasution and Ashar Hasairin

BS-007

39 - 45

The Growth of Orchid (Dendrobium sp) in In Vitro Giving with Coconut Water on
Different Medium
Fauziyah Harahap

BS-005

23 - 28

91
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Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

BS-011

Frequency Attendance of Makrozoobentos with Physics Factor and Chemistry in
Territorial Water of Babura River
Masdiana Sinambela and Mariaty Sipayung

BS-012

Liana Dwi Sri Hastuti, Jane Nicklin and Ameilia Zuiyanti Siregar
BS-013

94

Diversity and Abundance of Insect Pollinators in Different Agricultural Lands in
Jambi, Sumatera
Elida Hafni Siregar, Tri Atmowidi, and Sih Kahono

BS-015

93

Land Degradation in the Upstream of Deli Watershed in North Sumatera Based
on Soil Erosion Rate Prediction and Soil Erosion Hazard Level
Sumihar Hutapea; Ellen Lumisar Panggabean and Endang Sari Simanullang

BS-014

92

Inventory of Nematophagous Fungi in Sumatera Utara, Indonesia

95

Biodiversity of Leaf-and Planthopper (Hemiptera: Auchenorrhyncha) on Rice
Ecosystem at High Land Tapanuli Of North Sumatera-Indonesia
Binari Manurung, Puji Prastowo, and Erika Rosdiana

96

Chemical Science
CS-001

The Binding Behaviour and Conformation of Rhodobacter sphaeroides TSPO in
DDM and DPC Detergents
Nora Susanti, Joshua Sharpe and Krisztina Varga

CS-002

Synthesis, Crystal Structure and Magnetic Properties of the Spin Transition
System [Fe(pq)3](ClO4)2 Complex

Iis Siti Jahro, Djulia Onggo, Bohari M. Yamin, Ibrahim Baba and Nandang Mufti

CS-003

CS-004
CS-005

Joshua and Saharman Gea

110 - 114

Yusnaidar, Basuki Wirjosentono, Thamrin, and Eddiyanto

115 -119

Isolation and Characterization of α-Cellulose of Rice Leaves
Inhibitory Activity of Alkaloid of Extract Ethanol Ranti Hitam (Solanum blumei
Nees ex Blume) Fruit ON Leukimia L1210 Cancer Cells Growth

155 - 163

Biofuel Production from Hydrocracking MEFA of Rice Bran Oils Over Natural
Zeolit Supported Ni and Ni-Mo Metals
Junifa Layla Sihombing, Jasmidi, Nurmalis, Ahmad Nasir Pulungan, and Ratna Sari
Dewi

CS-013

148 - 154

Effect of Temperature and Composition Zeolite on the Performance MembraneZeolite for Separation of Ethanol-Water by Pervaporation
Ridwanto, Rahmat Nauli, Ani Sutiani, and Anny Sartika Daulay

CS-012

145 - 147

Preparation, Characterization and Activity Assay of NiO-CoO-MoO/Zeolite-Y
Catalyst on Hydrocracking of Cashew Nut Shell Liquid in Fixed-Bed Reactor
Ahmad Nasir Pulungan, Junifa Layla Sihombing, Hafni Indriati Nasution, Ratu
Evina Dibyantini, Rini Selly, Wega Trisunaryanti, and Triyono

CS-011

141 - 144

The Activity Values of Cla (Conjugated Linoleic Acid) Synthesized from Castor
Oil by Using Visible Spectrofotometer with DPPH as Free Radical
Marham Sitorus and Bajoka Nainggolan

CS-010

132 - 140

Compregnated Oil Palm Trunk (Elaeis guineensis Jacq.) with 20% Dammar
Resin (Agathis dammara)
Nurfajriani, Leni Widiarti, and Basuki Wirjosentono

CS-009

127 - 131

Study of Rubber Seed Oils Hydrocracking into Biogasoline and Diesel Fraction
Over the Combination Y-Zeolite and Ni Catalyst
Ary Anggara Wibowo, Salsabila Firdausyah, Siti Hajjah, Dina Dwiyanti, Junifa
Layla Sihombing, Ahmad Nasir Pulungan

CS-008

129 - 126

The Isolation of Nanocrystalline Cellulose from Palm Empty Fruit Bunches
Mahyuni Harahap, Fenny Aulia, and Saharman Gea

CS-007

103 - 109

The Utilization of Tamarillo Peels as Matrix of Bacterial Cellulose-Based
Nanopaper

Murniaty Simorangkir, Ribu Surbakti, Tonel Barus and Partomuan Simanjuntak
CS-006

97 - 102

Preparation of Kraft Lignin Based Polyol from Pulp Mill Black Liquor through

Universitas Negeri Medan
The Character Building University

164
165

6

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Oxypropylation Reaction
Abubakar, Basuki Wirjosentono, Thamrin, and Saharman Gea
CS-014 Characterization and Utilization of Bentonite Sabang of Aceh (Indonesia) as

Fillers Polymer Nanocomposite
Saharman Gea, Julinawati, and Basuki Wirjosentono

166

CS-015 Efficiency and Selectivity Improvement of the Silica Chitosan Hybrid by pH

Optimilization in Simultan Heavy Metals Mg(II) and Ni(II) Adsorbtion Using Solid
Phase Extraction Method
Lisnawaty Simatupang
CS-016 Natural Rubber Modification: Graft-Copolymerization of Cyclic Natural Rubber by

167

Free Radical Reaction with Maleic Anhydride
Eddiyanto, Alkhafi Maas Siregar, and Winsyahputra Ritonga

168

Mathematical Science
MS-001 An Active Constrained Based Approach for Solving Problems for Positioning

New Products Under Risk
Nerli Khairani

169 - 178

MS-002 An Integrated Optimization Model for River Water Quality to Estimate

Wastewater Removal
Syafari

179 - 188

MS-003 An Improved Approach for Solving the Plant Cycle Location Problem
Agusman

189 - 192

Physical Science
PS-001 A Comparison of Methods for Testing Homogeneity of Average Temperature and

Precipitation Series
Marzuki Sinambela and Esty Suryaningsih

193 - 200

PS-002 Mapping for Groundwater Potential Based on Resistivity Data Interpretation in

Pamah Paku Kutambaru Langkat Regency
Hengki Sembiring and Rita Juliani

201 - 209

PS-003 Lithology of Ketuken Watershed in Langkat
Rochayanti N R Simatupang and Rita Juliani

210 - 217

PS-004 Anthrophegenic Causes Analysis on Heavy Metal Pollution in River Water and

Sea Water in Middle Tapanuli Regency North Sumatera
Rahmatsyah, Eddy Marlianto, Mester Sitepu, and Motlan

218 - 227

PS-005 Morphological Analysis and Content Elements of Limestone from Village Sulkam

Langkat Using Scanning Electron Microscope (SEM)
Rita Juliani, Timbangen Sembiring, Mester Sitepu, and Motlan

228 - 238

PS-006 Influence of Concentration and Post-Heating to the Crystal Size and Optical

Properties of ZnO Thin Films
Nurdin Siregar, Eddy Marlianto, Saharman Gea, and Nurul Taufiqu

239 - 248

PS-007 Synthesis and Characterization Optical Properties of Cu2O Nanoparticles with

Coprecipitation Method Based Concentration Variations Precipitator
Pintor Simamora, Juan R. S., and Berton M. Siahaan

249 - 259

PS-008 Thermal Analysis and Structure of Nano Composite Palm Oil Boiler Ash
Eva Marlina Ginting and Nurdin Bukit

260 - 269

PS-009 Effect of Rice Husk Ash and Palm Oil Boiler Ash as a Mixture on Concrete

Porosity
Karya Sinulingga and Satria Mihardi

270 - 278

PS-010 Geoelectric Investigation Schlumberger Configuration of Limestone Distribution

in Cangap Kerabangen Area Kutambaru Subdistrict Langkat Regency
Rappel Situmorang and Sovian S.T. Sigiro
PS-011

8

279 - 288

To Determine the Geothermal Fluid and to Identify Geothermal Stones Mineral at 289 - 296
Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding:
The The
FirstFirst
International
Seminar
on Trends
in Science
and and
Science
Education
20142014
– ISBN
978-602-9115-37-6
Proceeding:
International
Seminar
on Trends
in Science
Science
Education
– ISBN
978-602-9115-37-6

Geothermal Area Tinggi Raja Simalungun, North Sumatera Province Using 2D
Resistivity Imaging and XRD
Muhammad Kadri and Eko Banjarnahor
PS-012

Correlation of Spectral Reflectance Characteristic Based on Spectrometer
Cropscam MSR 16R and Satellite Image Landsat TM (Study Case In MedanIndonesia)
Togi Tampubolon

PS-013

Yahya Darmawan, Esti Suryaningsih, and Lamtupa Nainggolan
PS-014

306 - 314

Influence of Sintering Time on the Properties of High Temperature
Superconductor BPSCCO Based
Eidi Sihombing

PS-015

297 - 305

Sensitivity of Breaks for Additive Seasonal and Trends (BFAST) Method to
Detect the Vegetation Changes Based on the Choice of Vegetation Indices and
Land Cover Types

315 - 319

Resistance Analysis of Rock and Mineral Under Surface by Using Geoelectric
Method in the Village of Dolok Marawa Simalungun Distric
Abd Hakim S and Marausaha Simanjuntak

320

Science Education
SE-001

Impact of Phet Simulation Media to Minimize Quantity Misconceptions Students
in Learning Dynamic Electrical Material
A.Halim, Azzarkasyi and Ibnu Khaldun

SE-002

Adriana Y.D. Lbn Gaol and Herbert Sipahutar
SE-003

Turnip Betty, Simanjuntak Mariati Purnama, and Purba Frikson

The Development of Media Device on Problem Based Learning Applied
Microbiology

SE-006

The Effectiveness of Using Developed Problem Based Learning Tools on
General Physics II of Physics Student, State University of Medan

Hasruddin and Mahmud

Jurubahasa Sinuraya, Sehat Simatupang, and Ida Wahyuni
SE-007

Application of Resource Based Learning Model for Improving Learning
Outcomes Student in the Cube and Beams Matter in Class VIII SMP Negeri 5
Stabat T.A. 2013/2014

SE-008

The Difference of Mathematical Problem Solving Achievement of Public Junior
High School Based on Learning Approach

SE-009

The Effect of Cooperative Learning Model Type Group Investigation on Student’s
Achievement of Static Fluid in Class XI of SMA Negeri 1 Perbaungan A.Y.
2013/2014

SE-010

Effect of Cooperative Learning Model Type Numbered Heads Together (NHT)
Assisted Animation Media of Student Learning Outcomes

Asrin Lubis

Ani Minarni

Derlina and Rikcy Almeda

Mariati Simanjuntak and Rebecca Sianturi

344 - 350

351 - 358

359 - 367

368 - 378

379 - 386

387 - 393

394 - 402

Effect of Project Based Learning Model with KWL Worksheet on Divergent
Thinking in Solved Physics Problems
Satria Mihardi and Karya Sinulingga

SE-012

338 - 343

The Effect of Problem Based Learning Model Toward Students’ Science Process
Skills in Senior High School

SE-005

SE-011

328 - 337

Misconception Reduction Effectiveness in Physics Learning Through Laboratory
Working Methods in the Concept of Temperature and Heat
A.Halim, Qusthalani and Ibnu Khaldun

SE-004

321 - 327

Misconception on Biology Materials Among Biology Teachers and Science
Students of Senior High School in North Sumatra

The Improvement of the Mathematical Problem Solving Ability at MTSN 2

Universitas Negeri Medan
The Character Building University

403 - 409
410 - 419

9

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Through Realistc Math Approach
Muhammad Arif Hidayat
SE-013

The Influence of Inquiry Training Learning Model Toward Students’ Achievement
on the Topic of Heat in Class X Semester II MAN Kisaran A.Y. 2013/2014
420 - 428

Sehat Simatupang and Ika Nurjannah Sirait
SE-014

Analysis Implementation of Practical Approriate with Standard of Competency
Biological Lesson and the Application at Class X in SMA Negeri 11 Medan
Tembung in Academic Year 2009/2010.

SE-015

Analysis of Competency and Performance of Secondary Teachers: Case Study
at Five Cities/Districts in South Sumatra

SE-016

An Intensive Study of Teaching Model of Quantum Physics at Study Program of
Physics Education in University

SE-017

The Effectivenees of Laboratory Experiment Method to Increase Activity and
Student’s Achievement on Teaching Salt Hydrolysis

429 - 436

Venisha E.A. Pardede and Tri Harsono

Umi Chotimah, Zahra Alwi, and Farida

437 - 445

Mara Bangun Harahap

446 - 452

Ramlan Silaban, Agustina M.L. Tobing, and Irving Josafat Alexander
SE-018

Sondang R Manurung
SE-019

461 - 469

The Use of Equation Worked Examples for Solving Electrochemistry Problem
Erdawati

SE-020

470 - 477

The Effect of Predict Observe Explain Strategy (POE) on Students Activity and
Learning Outcome on Human Respiratory System Sub Topic in Grade XI
Science Program at SMA Negeri 15 Medan Academic Year 2013/2014
Suyedi Hendra Yanto and Syahmi Edi

478 - 484

SE-021

The Difference Between the Ability of Students in Solving Problem by Applying
Cooperative Learning Type STAD With and Without the Help of Geogebra

SE-022

Effect of Creative Learning Techniques and Reasoning Ability Toward Student
Achievement in Physics

SE-023

Improving Speaking Ability of the Students in English Lessons by Using Learning
Model TPS (Think Pair Share) in Class V SD Immanuel Medan

SE-024

The Application of Quantum Model Learning to Improve Student Learning
Motivation on Science Subject State Class V SD 064 978 Medan Denai

Sinta Dameria Simanjuntak

485 - 493

494 - 507

Purwanto

408 - 517

Naeklan Simbolon, Fridawati R. Tambunan

Nani Barorah Nasution
SE-025

518 - 523

Group Investigation Assisted E-Learning: Assessing the Impact of Interactive
Media on Student’s Learning Achievement and Critical Thinking
524 - 532

Wenny Pintalitna and Herbert Sipahutar
SE-026

A Gametogenesis Module Development in POE (Prediction, Observation and
Explanation) Oriented Model
533 - 539

Hafizah Ilmi Sufa and Meida Nugrahalia
SE-027

Scientific Creativity in Learning Biology in Senior High School Tebing Tinggi City,
North Sumatra
540 - 546

Widya Arwita
SE-028

453 - 460

Contribution of Formal Thinking Ability on the Concept Mastery of Kinematics

Application of Cognitive Theory of Content on Learning Ability to Increase
Physical Science Using Generic Injection Needle Kit
547 - 556

Nurmaulita
SE-029

The Perceptions of Global Warming and Environmental Benefits of Biodiesel for
Sustainable Energy Among High School Students in Jakarta

SE-030

Ability Profile of Multiple Representations (MR) Students of Teacher Prospective
on Static Electricity Topic
566 - 571

Desnita

10

557 - 565

Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding:
The The
FirstFirst
International
Seminar
on Trends
in Science
and and
Science
Education
20142014
– ISBN
978-602-9115-37-6
Proceeding:
International
Seminar
on Trends
in Science
Science
Education
– ISBN
978-602-9115-37-6

Nurliana Marpaung and Liliasari
SE-031

Low Achievement of Indonesian Student in PISA and TIMSS Test Results and
the Related Factors
Ridwan Abdullah Sani

SE-032

The Effect of Guided Discovery Based Learning Model Towards Students
Learning Outcomes of Chemistry on Redox Reaction Concept
Anna Juniar, Dede Suriyani, Pravil Mistryanto, and Debby Masteriana

SE-033

616 - 625

Pengembangan Model Pembelajaran Hybridlearning Mata Kuliah Pengantar
Sosiologi di Universitas Negeri Medan
Trisni Andayani

SE-037

605 - 615

The Characteristics of Theology of Moslem Batak Toba
in State University of Medan
Ramli Nur, Usman Pelly, Hasan Bakti Nasution

SE-036

596 - 604

Evaluation of Sports Training Program Guidance Center Student (PPLP) North
Sumatra
Sabaruddin Yunis Bangun

SE-035

589 - 595

A New Breakthrough in Chemistry and Management
Wesly Hutabarat

SE-034

572 - 588

626 - 630

Pre Competency Test Standardization on Program of Field Experience HAT
Competency-Based Graduate Education Through Item Analysis
Pargaulan Siagian, Elvis Napitupulu, Arifin Siregar, Hudson Sidabutar

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1
1

Lampiran

LEMBAR
I{ASIL PEMLAIAN SEJAWAT SEBIDANG ATAU PEER KEVIEW
KARYA ILMIAH : PROSIDING INTERNASIONAL
Judul Makalah

:

Penulis Makalah

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Identitas Makalah

"The Growth Of Orchid (Dendrobium Sp) In
Water On Different Mediao'

In

Vitro Giving With Coconut

Fauziyah Harahap
a. Judul Prosiding

Abstract International Seminar

b.ISBN
c. Tahun Terbit
d. Penerbit
e. Jumlah halaman

Kategori Publikasi Makalah
(beri /pada kategori yang tepat)
Hasil Penilaian Peer Review

d
tl

December 2014

FMIPA Unimed
60 B-09

Prosiding Forum Ilmiah Internasional
Prosiding Forum Ilmiah Nasional

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Padang,

20

0CT

2015

Reviewer - I
itas Andalas

irfrizal Sy
71993091001

Prof. Dr. Syamsuardi, M.Sc
NrP. 1961091 0l 98901 1001
Unit kerja : Jurusan Biologi
Universitas Andalas, Padang

I

8

Lampiran

LEMBAR
HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW
KARYA ILMIAH : PROSIDING INTERNASIONAL
Judul Makalah

:

Penulis Makalah

:

Fauziyah Harahap

Identitas Makalah

:

a. Judul Prosiding
b. ISBN
c. Tahun Terbit
d. Penerbit
e. Jumlah halaman

Kategori Publikasi Makalah
(beri /pada kategori yang tepat)
Hasil Penilaian Peer Review

Of Orchid (Dendrobiuru Sp) ln
Water On Different Media"

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14,(
Medan,

20

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2015

Reviewer - 2

'i/
'-

NIP.

1

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m, M.Sc

Prof.

026 199103 1001

NIP

1

in Ilyas,M.Biomed
199203 1003

Universitas Sumatera Utara

8

Lampiran

LEMBAR
HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW
KARYA ILMIAH : PROSIDING INTERNASIONAL
Judul Makalah

:

Penulis Makalah

:
:

Identitas Makalah

'orhe Growth of orchid (Dendrobium Sp) rn
Water On Different Media"

In vitro Giving with

coconut

Fauziyah Harahap
a. Judul Prosiding

Abstract Intemational Seminar

b.ISBN
c. Tahun Terbit
d. Penerbit
e. Jumlah halaman

Kategori Publikasi

Makalah p]
[-l

(beri /pada kategori yang tepat)
Hasii Penilaian Peer Review

Decernber 2014

FMIPA Unimed
60 B-09

Prosiding Forum Ilmiah Internasional
Prosiding Forum Ilmiah Nasional

:

Nilai Maksimal Prosidins
Komponen
Yang Dinilai

lnternasional

Nasional

I

VA
a. Kelengkapan unsur isi artikel (10%)

Nilai Akhir
Yang
Diperoleh

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b. Ruang lingkup dan kedalaman pembahasan (30%)

7,?

c. KecuKupan dan Kemutahran datalrntormasi dan

metodologi (30%)

3,?

d. Kelengkapan unsur dan kualitas penerbit (30%)

7,9

Totat = (100%)

17
Medan,

September 2015

Reviewer -

3

Prof. Dr. Herbert Sfahutar, MS, M.Sc
NIP. 19610626 198710 1001
Unit kerja : Guru Besar MIPA I-INIMED

8

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

BS-004

THE GROWTH OF ORCHID (Dendrobium sp) IN IN VITRO GIVING WITH
COCONUT WATER ON DIFFERENT MEDIUM
Fauziyah Harahap
Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Medan,
Jln. Willem Iskandar Psr V Medan Estate, 20221, Indonesia
E-mail: fauziyahharahap@gmail.com

ABSTRACT
This study aimed to determine the effect of coconut water on the growth of in vitro orchid
shoots (Dendrobium Sp) on Murashige and Skoog medium, medium containing foliar fertilizer
(Grow More (GR) = N: P: K = 20: 20: 20). Research has been carried out in the Laboratory of
"YAHDI" Plant Tissue Culture and Biology Departement of Unimed. This research design using
non factorial completely randomized design with treatments namely: The first setting: MS
Medium with 6 levels coconut water, namely 0%, 5%, 10%, 15%, 20%, 25%, the second :
Medium containing Grow More (GR) 2 g/l with 6 levels coconut water, namely 0%, 5%, 10%,
15%, 20%, 25%. Each combination treatment was repeated 4 times. Parameter observations in
this study was : the number of shoots, number of leaves, number of roots, shoots performance.
The results showed that there are significant effect of medium, coconut water to all parameters:
the number of shoots, number of leaves and number of roots of the Dendrobium orchid. Shoots
performance was pale green shoots for giving of low doses of coconut water, will be green with
coconut water with medium doses, the higher the concentration of coconut water will make the
shoots appear stunted and eventually died.
Keywords: MS medium, coconut water, Dendrobium Sp, Grow More

INTRODUCTION
Orchids belonged flowering plants, widespread in parts of the world, including
Indonesia. Indonesia is a country that has the number and types of orchids are very diverse.
Donations and participation of Indonesian Orchid in the Orchids world is a big. It is estimated,
orchid species spread throughout in the world amounted to 20,000 species and 6.000 species
of which are in the Indonesian. Besides orchid species, we known also some orchids in the
cross or hybrid. It is estimated that annually, produced 1.000 new hybrid. Orchids have a high
economic value when compared to other ornamental plants (BPPP, 2004)
Dendrobium is one of the family Orchidaceae plants are much favored by consumers.
Popularity of this orchids is due to the variety of colors and the beautiful flowers, fresh flowers
also caused a period longer than other ornamental plants (Amalia, 2013). Dendrobium orchids
are plants from tropical regions that require sunlight and temperatures hot enough, not like a
particular orchid appropriate only in cold regions like Paphiopedillum. Dendrobium need

46

Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

temperature 28-30oC (a minimum temperature of 15oC), with light is 50-60%. Dendrobium need
environment is not too wet, but require high humidity at 65% - 70% (Kartiman, 2004).
The genus Dendrobium has a high diversity on habitat, size, pseudobulb, leaf and
flower color. Spread spectrum is wide, from coastal areas to the mountains. Spread in India, Sri
Lanka, China, Japan, Southeast Asia, to the Pacific region, Australia, New Zealand, and Papua
New Guinea. It grows well at altitude 0-500 m above sea level with 60-80% humidity (Linga, P
and Marsono. 2001).

Simpodial type
Dendrobium
orchid

Dendrobium with the
long pseudo bulb dan
flower

Figure 1. Ferformance of Dendrobium
This orchid has a long pseudo bulb. Leaf intersect along the pseudo bulb. According to
the nature of the growth of this orchid including simpodial types, forming clumps and roots in
every pseudo bulb. The flowers often appear at the apical bud. However, in mature plants, the
flowers some time appear in the axilar.
Tissue culture method was developed to help reproduce plants, especially for plants
that are difficult to breed in generative. Plantlet produced from tissue culture has several
advantages, namely, identical to the parent, can be reproduced in large quantities, not to need
a large place, producing a large plantlet in a short time, plantlet quality is guaranteed, and the
seedlings grow faster compared with conventional propagation (Anonymous, 2010).
Tissue culture is a plant propagation technique that greatly influenced by the content of
the substante in medium. To maximize the growth process, used hormones or plant growth
regulators. Plant growth regulator (PGR) has a role in the process of growth and development
for the survival of a plant (Nugroho, 2000, Harahap, 2011).
Coconut water is the liquid endosperm or food reserves of energy sources in the
coconut. It is a complex organic substance that is always used in plant propagation by tissue
culture techniques. This complex substance is often used, but have not obtained the right dose
and has not verified the content of the materials that exist in the coconut water.
Universitas Negeri Medan
The Character Building University

47

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

Coconut water, indicated to contain high endogenous cytokines, can induce the plant
shoots. Recent research get content of coconut water, namely: amino acids, organic acids,
nucleic acids, purines, sugars, alcohols, vitamins, minerals, growth regulators. Coconut water
contain plant growth regulator (Gunawan, 1992): 9-B-D ribofuranosyl zeatin, Zeatin, N-NDiphenyl urea, 2 (3-methyl-but-2 etyl amino) 6-purin one. The content of growth regulators in
coconut water is beneficial to induce callus and induce morphogenesis process (Pisesha,
2008).
Growmore is a foliar fertilizer that is always used for spraying on orchid plant.

This

fertilizer contains N : P : K with ratio 40: 20: 20. Generally to stimulate vegetative growth
(Fahruroh, 2008). In this study Growmore replaces of MS medium for plant tissue culture. In the
commercial treatment, using growmore can be saving the operational cost. So that the potential
for business development. This fertilizer is a potential can be developed through this research,
because growmore containt high Nitrogen that needed for orchid growth. Nitrogen is a nutrient
that is needed primarily for vegetative growth. Plants absorb N is generally in the form of
ammonium (NH4+) and nitrate (NO3). Excessive N application will be effect to vegetative growth,
leaf color becomes dark green and more succulent (Fahruroh, 2008).
The purpose of this study was to determine the growth of in vitro orchid shoots after
giving the Murashige Skoog (MS), Growmore (GR) medium and added coconut water.

METHODOLOGY
This research was conducted at the YAHDI Laboratory of Tissue Culture, Medan, and
Biology laboratory UNIMED Medan, North Sumatera. Indonesia. The materials used are in vitro
Dendrobium Sp plantlets, MS medium, Growmore, alcohol, sterile distilled water, detergent,
coconut water.
Standard tissue culture tools were used for this research. This research design using
non factorial completely randomized design with treatments namely: The first setting: MS
Medium with 6 levels coconut water, namely 0%, 5%, 10%, 15%, 20%, 25%, the second:
Medium containing Grow More (GR) 2 g/l with 6 levels coconut water, namely 0%, 5%, 10%,
15%, 20%, 25%. Each combination treatment was repeated 4 times.
Shoots were planted on the shoot induction medium according to the treatment. The
culture was maintained at 24°C by regulating the room air conditioner. For maintaining the light,
flourescent light of 3000-3200 lux were applied and set to 16 hour photoperiod. the number of
shoots, number of leaves, number of roots, shoots performance were observed weekly from 1
until 12 weeks after planting. Data analyzed with ANAVA non factorial and differences test with
Duncan's multiple range test (DMRT) at the level of 95%.
48

Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

Table 1. Combination of treatmen between MS medium with coconut water, Growmore
medium and coconut water
Medium
Coconut
water (v/v)
0 ml
50 ml
100 ml
150 ml
200 ml
250 ml

Murashige Skoog (MS)
M1C0
M1C1
M1C2
M1C3
M1C4
M1C5

Medium Coconut
water (v/v)

GrowMore (GR)

0 ml
50 ml
100 ml
150 ml
200 ml
250 ml

G1C0
G1C1
G1C2
G1C3
G1C4
G1C5

The first combinatios of medium is MS medium with coconut water:
MS + 0% coconut water (M1C0)
MS + 5% coconut water (M1C1)
MS + 10% coconut water (M1C2)
MS + 15% coconut water (M1C3)
MS + 20% coconut water (M1C4)
MS + 25% coconut water (M1C5)
The second combination of medium is Growmore (GR) with coconut water:
GR + 0% coconut water

(G1C0)

GR + 5% coconut water

(G1C1)

GR + 10% coconut water (G1C2)
GR + 15% coconut water (G1C3)
GR + 20% coconut water (G1C4)
GR + 25% coconut water (G1C5)

RESULTS AND DISCUSSION
Results of analysis of variance showed a significantly different effect (significant)
between the treatment given to the value of the all parameters: the number of shoots, number of
leaves and number of roots of the Dendrobium orchid, shoot performance.
Table 2. Number of Shoots, Leaves and Roots of Dendrobium Sp after Coconut Water
Treatment in MS Medium and Growmore medium

Coconut
water (%)
0
5
10
15
20
25

Combination treatment
Mean of shoot number
Mean of leave number
MS
Growmore
MS
Growmore
Medium
Medium
Medium
Medium
4.75
3
4
2.5
5.5
5.25
4.75
2.25
5.75
3.75
3.75
4
6.75
4
3.5
2.75
5.5
5.5
3.75
3.5
3.5
6.5
2.75
2.75

Universitas Negeri Medan
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Mean of root number
MS
Growmore
Medium
Medium
0.5
0
1
0
0.75
0
0
0
0
0
0
0

49

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

a. Number of Shoot
Results showed that MS medium plus 15 % coconut water produces shoots grew
100%, the highest number of shoots (6.75). While on treatment with growmore, required a
concentration of 25% coconut water to produce the highest number of shoots (6.5). On
treatment with MS medium, it appears that the number of shoots increased with increasing
doses of coconut water until a certain dose (15%), but decreases with increasing doses of
coconut water (20%), with the number of shoots 5.5. On treatment with growmore, seen a trend
of increasing number of shoots, up to the highest dose given (25% coconut water) by the
number of shoots 6.5.
It is understandable that the nutrient content on MS medium is much more complete
than in the Growmore, so that the giving of 15% coconut water has stimulate the growth of
shoots. But with growmore added 25% coconut water also produces a high number of shoots.
This means that coconut water is potential to stimulate growth in the number of shoots on
Dendrobium orchids. Paramartha (2012) said that any effect of addition of NAA and BAP
combination on growth and development of Dendrobium taurulinum JJ Smith Seeds in vitro.
Rostika (2008) reported that the highest number of mangosteen axillary bud formation was
obtained on the medium containing 3 mg/l BA (Benzyl Adenine) . BA is one of cytokinines
hormone.
b. Number of Leaves
In the treatment of MS and with Growmore medium, it appears that the number of leaves
increased by giving low doses of coconut water (5% on MS medium and 10% in Growmore
medium), but decreases with increasing doses of coconut water. Surachman (2011) give the
coconut water for the in vitro propagation and increasing the Patchouli leaves number.

Figure 2. Number of Leaves of Dendrobium Sp after Coconut Water Treatment in MS Medium
and Growmore medium

50

Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

c. Number of Roots
In the treatment of MS medium with a low dose of coconut water (0-10%) can produce
roots (average 0.75), but with increasing doses of coconut water (15%), roots are not formed.
On treatment with growmore, the addition of coconut water did not produce roots.

1. MS

2. MS+ CW 5%

3. MS+ CW 10 %

4. MS+ CW 15 %

5. MS+ CW 20 %

6. MS+ CW 25 %

7. GR

8. Gr+ CW 5 %

9. Gr+ CW 10 %

10. Gr+ CW 15 %

11. Gr+ CW 20 %

12. Gr+ CW 25 %

Figure 3. Performance of Dendrobium after 12 weeks planting. In the picture shows that the
orchid plant with medium MS + 5 % coconut water, has a lot of shoots and leaves.
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The Character Building University

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Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency
Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

This study proved that coconut water can stimulate the increase in the number of
shoots, number of leaves, but can not stimulate the increase in the number of roots. Can be
understood as organic matter (plant growth regulator) contained in coconut water is

a

cytokines, the role of potential to stimulate the growth of shoots not for improve the roots
(Harahap, 2011, 2012, Gunawan, 1997). Pisesha, (2008) said that any effect of concentration of
IAA, IBA and coconut water against root formation Poinsettia (Euphorbia pulcherrima Wild. Et.
Klotz) in vitro. IBA concentration and the period of pulse treatment have significant effects on the
average number of roots produced per shoot on Catharanthus roseus (Rupesh, 2013).
d. Performance of Shoots
Shoots performance was pale green shoots for giving of low doses of coconut water, will
be green with coconut water with medium doses, the higher the concentration of coconut water
will make the shoots appear stunted and eventually died.
Coconut water contains inorganic ions (chlorine, copper, iron, magnesium, phosphorus,
potassium, sodium, sulfur), nitrogen component (thanolamine and ammonia), amino acids and
related substances, enzymes, organic acids. In addition, coconut water also contains sugar
between 1.7 to 2.6% and protein from 0.07 to 0.55%. Besides being rich in minerals, coconut
water also contains a variety of vitamins such as citric acid, nicotinic acid, pantothenic acid, folic
acid, niacin, riboflavin, and thiamine.
The type and concentration of Plant Growth Regulator (PGR) was a component that
determines the success of tissue culture. The explants were cultured on MS medium,
Growmore medium, for further propagation, using coconut water was a class of cytokines that
role in cell division, stimulate the multiplication of shoots, stimulate DNA synthesis process
(Harahap, 2011)

CONCLUSION
There are significant effect of medium, coconut water to all parameters: the number of
shoots, number of leaves and number of roots of the Dendrobium orchid. Shoots performance
was pale green shoots for giving of low doses of coconut water, will be green with coconut water
with medium doses. Dendrobium needed medium doses (10-15%) coconut water for improve
the shoot number and the leave number. The higher the concentration of coconut water will
make the shoots appear stunted and eventually died.

52

Universitas Negeri Medan
The Character Building University

Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

REFERENCES
Amalia,. N.N,. (2013), Effect of Type and Vitamins Concentration on the Growth and
Development of Dendrobium Seeds laxiflorum JJ Smith in In Vitro. Journal of Science
and Arts POMITS, 1: 1-6
BPPP (Badan Penelitian dan Pengembangan Pertanian, (2004), Teknologi agribisni tanaman
hias, Jakarta, Balai Penelitian Tanaman Hias. Pusat Penelitian dan Pengembanga
Hortikultura.
Fahruroh, W., (2008), Effect of Growmore fertilizer (20:20:20) And Quick Grow of Adenium F
on Growth entres (Adenium obesum) After trimming, Bogor, Thesis, Faculty of
Agriculture, Bogor Agricultural University.
Harahap, (2011). Kultur Jaringan Tanaman. UNIMED Press. Medan.
Harahap (2012). Pertumbuhan Tunas Manggis In Vitro (Garcinia-Mangostana L.) Hasil
Perlakuan Zat Pengatur Tumbuh Benzyl Adenine dan Ukuran Eksplan yang Berbeda.
Saintika Journal, Vol 12: 1-13.
Kartiman, R., (2004), Effect of combinations of growth regulators and pieces protocorm like
bodies for the propagation of the giant moon orchid (Phalaenopsis gigantea) with tissue
culture methods. Thesis, Faculty of Agriculture, Bogor Agricultural Institute.
Paramartha, A., EN, (2012), Effect of Concentration Addition PGR combination Naa And Bap
on Growth and Development of Dendrobium Taurulinum JJ Smith Seeds In Vitro. Journal
of Science and Arts of ITS, 1 (1):: 2301-928X.
Pisesha, (2008), Effect of IAA concentration, Iba, Bap, And Coconut Water Against Root
Formation Poinsettia (Euphorbia pulcherrima Wild. Et. Klotz) In Vitro, Thesis, Faculty of
Agriculture, Bogor Agricultural Institute.
Rostika I, Nopianti S, Ika M. (2008). Micropropagation of mangosteen (Garcinia-mangostana
L.). Indonesian Journal of Agriculture 1:28-33
Rupesh KR, Naresh KS, and Vandana S, (2013). Effect of plant growth regulators on
micropropagation of Catharanthus roseus. International Journal of Advanced
Biotechnology and Research 4:986-993.
Surachman, D., (2011), Teknik Pemanfaatan Air Kelapa Untuk Perbanyakan Nilam Secara In
Vitro, Buletin Teknik Pertanian, 16 (1): 31-33..

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