BGRI
•
Proceedings
Borlaug Global Rust Initiative
2012 Technical Workshop
September 1–4
Beijing, China
Poster Abstracts
Theme 4: Breeding for Rust Resistance
Edited by Robert McIntosh
bgri@cornell.edu
www.globalrust.org
ISBN: 13: 978-0-615-70429-6
© 2012 individual authors and the Borlaug Global Rust Initiative.
Do not reprint without express permission from principal author.
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Proceedings
BRGI 2011 Technical Workshop
Table of contents
Poster Abstracts
Breeding for Rust Resistance
Initiation of genomic recurrent selection for slow-rusting adult plant resistance to
stem rust in wheat
J. Rutkoski, R. P. Singh, J. Huerta-Espino, S. Bhavani and M. E. Sorrells .............................................................. 167
Identification of high yielding, drought tolerant and stripe rust resistant
facultative/winter wheat genotypes at ICARDA
W. Tadesse and S. Rajaram ................................................................................................................................................ 168
Stripe rust and leaf rust responses of a double haploid wheat population derived
from Ducula-4/*Brookton
M. S. Saharan, G. Singh, R. Tiwari, T. L. Setter, V. Panwar and I. Sharma ............................................................. 169
Global variation of winter wheat in response to Pst race Ug99
A. Morgounov, B. Akin, Y. Kaya, M. Keser, L. X. Yu, Y. Jin, S. Martynov, Z. Mert, M. Sorrels
and R. Wanyera ...................................................................................................................................................................... 170
Studies of wheat species collections for resistance to harmful diseases
E. D. Kovalenko, T. M. Kolomiets, M. I. Kiseleva, L. F. Pankratova and H. Bockelman ..................................... 171
Selection of wheat cultivars partially resistant to leaf rust and Septoria diseases
T. M. Kolomiets, E. D. Kovalenko, M. I. Kiseleva, L. F. Pankratova and H. Bockelman ..................................... 172
Phenotyping and genotyping of “Arsenal” accessions for resistance to Puccinia species
I. F. Lapochkina, A. I. Morgunov and G. Woldeab....................................................................................................... 173
Resistance to stripe rust in promising lines and bread wheat cultivars
M. Khodarahmi, A. Omrani and F. Afshari ..................................................................................................................... 174
Screening for stem rust resistance (race Ug99) in East Africa
S. Bhavani, P. Njau, R. Wanyera, A. Badebo and B. Girma ........................................................................................ 175
Developing single seed descent populations for combining biotic and abiotic stress
tolerances in bread wheat
G. Singh, M. S. Saharan, T. L. Setter, M. K. Singh and I. Sharma ............................................................................. 176
Evaluation of stem rust resistant wheat lines for commercial production in Kenya
P. Njau, L. Karani, S. Bhavani, R. Wanyera, J. Huerta-Espino and R. P. Singh ..................................................... 177
The importance of yellow (stripe) rust on wheat in Algeria and release of varieties
resistant to yellow rust and stem rust
A. Benbelkacem, N. Derbal and C. Djenadi .................................................................................................................. 178
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Resistance to threatening of Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici
races in Moroccan bread wheat cultivars and landraces
A. Ramdani, H. Ouabbou, S. Bhavani, K. Nazari, R. Wanyera, S. Lhaloui, F. Abbad-Andaloussi,
N. Nsarellah, S. Bennani, J. Haddouri and S. M.Udupa ............................................................................................. 179
Seedling and adult-plant responses of Iranian wheat genotypes to Sr31-virulent
Pgt races
F. Afshari, M. Aghaee, K. Nazari, M. Patpour, M. Esmaeilzadeh, G. Najafian, A. Yazdansepas, M.
Vahabzadeh, S. Mahfouzi, M. Khodarahmei, A. Amini, M. Mohammadei, A. R. Mohammadi,
M. R. Jalal Kamali, R. Wanyera and S. Bhavani ............................................................................................................. 180
Parsi, Sivand and Sirvan: Three new rust resistant wheat varieties for irrigated
temperate areas of Iran
G. Najafian, H. Amin, M. Dadaein, E. Pazhoumand, A. R. Nikzad, Sh. Sarikhani, R. Nikooseresht,
A. Jafarnezhad, A. Ghandi, D. Afuni, M. Khodarahmi, Gh. H. Ahmadi, A. Amini, H. Abdi, J. Hassanpour,
B. Shabanzadeh, A. Z. Faizabadi, F. Afshari, A. Malihipour, A. Zakeri, M. Yassaei, S. Rajaei,
M. Atahossaini, R. Aghnoum and S. M. T. Tabatabaei ............................................................................................... 181
Breeding for resistance to Pgt race Ug99 in Kazakhstan by application of molecular
markers
A. Kokhmetova, F. Ogbonnaya, M. Atishova, A. Morgounov and Sh. Rsaliev ................................................... 182
Breeding rust resistant wheat in Kyrgyzstan
M. Dzhunusova, A. Morgounov and A. Yahyaoui .................................................................................................... 183
Identification combined resistance to stripe rust and stem rust in Uzbek wheat
germplasm
Z. M. Ziyaev, R. C. Sharma, A. I. Morgounov, A. A. Amanov and Z. F. Ziyadullaev ........................................... 184
Agronomic performance of winter wheat genotypes resistant to Pgt race Ug99 in
Kenya and stripe rust in Uzbekistan
S. Islomov, R. C. Sharma, Z. Ziyadullaev, Z. Ziyaev and Z. Khalikulov ................................................................. 185
Stripe rust responses of wheat varieties released in Azerbaijan
M. Ahmadov .......................................................................................................................................................................... 186
Stem rust responses of Azerbaijani bread and durum wheat accessions
M. Abbasov, R. Bowden, J. Raupp, S. Sehgal, S. Babayeva and B. Gill ................................................................. 187
Reactions of Turkish wheat cultivars and breeding lines to Pgt race Ug99: 2006 - 2011
Z. Mert, K. Akan, L. Çetin, R. Wanyera, S. Bhavani, D. Singh and F. Düşünceli .................................................. 188
Winter bread wheat breeding studies in the transitional rainfed zone of Turkey
S. Belen, N. Bolat, M. Çakmak, A. Yorgancilar and A. T. Kilinç ................................................................................. 189
Wheat rust virulence in southern Russia
G. V. Volkova, O. F. Vaganova, E. V. Sinyak and Y. V. Shumilov .............................................................................. 190
Evaluation of stem rust responses in a spring wheat collection and lines with Sr genes
in the southern forest-steppe zone of Western Siberia
V. P. Shamanin, A. I. Morgounov, M. A. Levshunov, Y. I. Zelenskiy, Sh. Rsaliev, I. V. Pototskaya
and S. L. Petukhovskiy ......................................................................................................................................................... 191
Field responses of near-isogenic lines and wheat varieties to leaf rust in Bangladesh
K. Mustarin, M. M. A. Reza, M. S. Hossain, M. A. Hakim, P. K. Malaker, B. K. Goswami, M. M. Rahman,
N. C. D. Barma, M. M. Rahman, F. Ahmed and R. Islam ............................................................................................ 192
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Evaluation of wheat lines for resistance to stripe (yellow) rust in mid-hills of Nepal
S. Adhikari, S. M. Shrestha, G. B. Khatri-Chhetri, S. Sharma and R. S. Poudel .................................................... 193
Stem rust resistance in wheat lines from Bangladesh and Nepal
Z. A. Pretorius, R. Prins, P. K. Malaker, N. C. D. Barma, M. A. Hakim, D. Thapa, R. F. Park and G. L. Cisar ... 194
Elite wheat germplasm with resistance to stripe rust and stem rust for the Indian
Northern Hills Zone
V. Rana, S. C. Bhardwaj and S. K. Rana ............................................................................................................................ 195
The National Coordinated Wheat Program in Pakistan
M. Y. Mujahid, M. A. Khan H. Makhdoom, A. Bari, M. Tila, H. Manzoor, M. Tariq, A. Arian, A. Muneer,
A. Rattu, J. I. Mirza, K. Shafiq and R. Ward ..................................................................................................................... 196
Resistant wheat varieties can mitigate the Pgt race Ug99 threat to food security
A. K. Joshi, M. Azab, M. Mosaad, M. Moselhy, R. Sharma, S. Gelalcha, G. Bedada, M. R. Bhatta,
N. R. Gautam, S. R. Updahyay, N. C. D. Barma, D. B. Pandit, A. Hakim, P. K. Malaker, M. E. Haque,
T. P. Tiwari, A. Majid, M. Y. Mujahid, Z. Bishaw, T. Payne, R. P. Singh and H. J. Braun ..................................... 197
Assessment of partial resistance to stripe rust in elite wheat lines*
S. A. Safavi and F. Afshari .................................................................................................................................................... 198
Effect of stripe (yellow) rust on yield components of barley cultivars with race-specific
and slow rusting resistances*
S. A. Safavi, A. B. Ahari, F. Afshari and M. Arzanlou .................................................................................................... 199
Slow stripe rusting resistance in Iranian barley*
S. A. Safavi, A. B. Ahari, F. Afshari and M. Arzanlou .................................................................................................... 200
Effect of temperature on resistance to stripe (yellow) rust on wheat*
R. Bryant, C. Uauy, S. Dorling, L. Boyd and C. Ridout ................................................................................................. 201
Ethiopian farmers’ preferences for attributes of bread wheat: A gender-responsive
conjoint analysis*
K. Nelson, S. Davidson, R. Nelson, Y. Chiche, L. Sperling, S. Hunduma and S. Abebe .................................... 202
Combining resistance to leaf rust and terminal heat tolerance in wheat for the Eastern
Gangetic Plains of India*
C. Tiwari, B. Arun, V. K. Mishra, R. Dhari, U. Kumar, H. Wallwork and A. K. Joshi .............................................. 203
Evaluation of barley germplasm for seedling and adult plant responses (APR) to Pgt
race Ug99*
M. Wamalwa, J. Owuoche, R. Wanyera and S. Bhavani ............................................................................................ 204
Evaluation of Nepalese wheat genotypes for seedling and adult plant resistance to
leaf rust*
S. Baidya, S.C. Bhardwaj, M. Prashar, H. K. Manandhar, S. Sharma, S. K. Shrestha and A. K. Joshi .............. 205
Breeding for resistance to Mycosphaerella graminicola in wheat
S. M. Tabib Ghaffary, J. D. Faris, T. L. Friesen, O. Robert, V. Laurent, P. Lonnet, E. Margalé,
T. A. J. van der Lee, R. G. F. Visser and G. H. J. Kema .................................................................................................. 206
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative • globalrust.org
Initiation of genomic recurrent selection for slow-rusting adult plant resistance to stem rust
in wheat
J. Rutkoski1, R. P. Singh2, J. Huerta-Espino3, S. Bhavani4 and M. E. Sorrells1
1
Department of Plant Breeding and Genetics, 240 Emerson Hall, Cornell University, Ithaca, NY
14853, USA; 2CIMMYT, Apdo. Postal 6-641, 06600 El Batan, Mexico; 3Campo Experimental,
Valle de México INIFAP, Apdo. Postal 10, 56230 Chapingo, Edo de México, Mexico;
4
CIMMYT, ICRAF House, United Nations Avenue, Gigiri, Village Market-00621, Nairobi,
Kenya. Email: rutkoski.jessica@gmail.com
Genomic selection (GS) is a new breeding technology that promises to increase the rate of
genetic gain for quantitative traits. With GS, a prediction model for the trait(s) of interest is
developed using a relevant population with existing phenotypic and genotypic data. The
prediction model is then applied to a new set of breeding lines which have been genotyped in
order to estimate the breeding values. Based on the predicted breeding values, lines can be
selected for advancement or crossing to initiate the next selection cycle. This allows selection to
occur before phenotypic information for the lines can be generated, therefore enabling faster
selection cycles. In order to empirically evaluate the efficiency of GS relative to phenotypic
selection (PS) for slow-rusting adult plant stem rust resistance (APR), two parallel recurrent
selection (RS) schemes are underway, and are currently at the first selection cycle stage.
Selections are based on phenotypes in the first scheme (RS-PS), and on genotypes and predicted
breeding values in the second scheme (RS-GS). Based on cross-validation using the model
training population, we expect the selection accuracy with GS to be 0.6. Based on phenotypic
data from the same germplasm evaluated across four growing seasons, we expect the selection
accuracy with PS to be 0.78. Because the minimum selection cycle duration is 8 months with GS
and 12 months with PS, considering our estimates of selection accuracy we expect to achieve
1.15 times more genetic gain per unit of time with GS compared to PS.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
167
Borlaug Global Rust Initiative • globalrust.org
Identification of high yielding, drought tolerant and stripe rust resistant facultative/winter
wheat genotypes at ICARDA
W. Tadesse and S. Rajaram
Wheat breeding, ICARDA, P.O. Box 5466, Aleppo, Syria. E-mail: w.tadesse@cgiar.org
Stripe (yellow) rust and drought are the principal yield limiting factors for wheat production in
the CWANA region. ICARDA’s germplasm development approach is to identify genotypes with
high yield potential, high water use efficiency and resistance to stripe rust. Our aim is to
minimize and maximize yield gains during drought and good seasons, respectively. Toward these
objectives we make more than 500 crosses annually, and evaluate the segregating populations
using a modified pedigree/selected bulk approach in Pst-inoculated irrigated nurseries.
Preliminary and advanced yield trials are evaluated under both irrigated and rain-fed conditions.
With this approach we have identified stripe rust resistant genotypes (5-10% severity) which
yield as high as 6.5 and 2.5 t/ha under irrigated (450 mm) and rain-fed (232mm) conditions,
respectively. These genotypes combine high yield potential, drought tolerance, stripe rust
resistance and good grain quality. Some of the genotypes are candidates for release in some
countries.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
168
Borlaug Global Rust Initiative • globalrust.org
Stripe rust and leaf rust responses of a double haploid wheat population derived from
Ducula-4/*Brookton
M. S. Saharan1, G. Singh1, T. L. Setter2, V. Panwar1 and I. Sharma1
1
Directorate of Wheat Research, Karnal, Haryana, India; 2Department of Agriculture and Food,
South Perth, WA 6151, Australia. Email: mssaharan7@yahoo.co.in
Rusts are a threat to sustainable food production. In India leaf rust is a regular occurrence, but
stripe rust can be more damaging even though it is less widespread. New sources of resistance
are required to combat the regular emergence of new races. In the present study, 155 double
haploid lines from Ducula-4/2*Brookton were screened against prevalent races of Pst (78S84,
46S119) and Pt (12-2, 77-2, 77-5, 104-2) at Karnal during 2009 - 2011. Lines were categorized
by Area Under the Disease Progress Curve (AUDPC) values calculated from the rust severities
recorded at regular intervals. Among 155 lines, 32 (group I) were stripe rust-free whereas other
lines gave AUDPC values of 1-100 (group-II, 7 lines), 101-200 (group-III, 21 lines) and 201-500
(group-IV, 38 lines); the remaining lines gave higher values. For leaf rust, 20 lines were rust-free
and 32, 9 and 52 lines were placed in groups II, III and IV, respectively. Group II - IV lines for
both diseases were considered to be slow rusting as their AUDPC values were less than 20% of
the checks. Such slow rusting lines can be used as parents for incorporating slow rusting genes
into breeding populations.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
169
Borlaug Global Rust Initiative • globalrust.org
Global variation of winter wheat in response to Pst race Ug99
A. Morgounov1, B. Akin1, Y. Kaya2, M. Keser3, L. X. Yu4, Y. Jin5, S. Martynov6, Z. Mert7, M.
Sorrels4 and R. Wanyera8
1
CIMMYT, P.O. Box 37, Emek, Ankara 06511, Turkey; 2International Agricultural Research
Institute, P.O. Box125, Karatay, Konya, Turkey; 3ICARDA, P.O. Box 37, Emek, Ankara 06511,
Turkey; 4Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853,
USA; 5USDA-ARS Cereal Disease Laboratory, 1551 Lindig Street, University of Minnesota, St.
Paul, MN 55108, USA; 6Vavilov Institute, 44 B. Morskaya St., St. Petersburg, Russia; 7Central
Field Crop Research Institute, Sehit Cem Ersever, Caddesi No:9-11, Ankara, Turkey; 8Kenya
Agricultural Research Institute, P.O. Private Bag 20107, Njoro, Kenya. Email:
a.morgounov@cgiar.org
The International Winter Wheat Improvement Program (www.iwwip.org) is was established as a
joint project by the Ministry of Food, Agriculture and Animal Husbandry of Turkey, CIMMYT,
and ICARDA in 1986. The objective of the program is to develop facultative and winter wheat
germplasm for the Central and West Asia region. IWWIP also facilitates global germplasm
exchange of winter wheat by assembling lines from various sources, and evaluating and
distributing selected entries through its system of international nurseries. Resistance to rusts is a
high priority along with adaptation and grain quality. Due to the obvious importance of Pst race
Ug99, evaluation of germplasm for resistance in Kenya started in 2008. From 2008 to 2011,
approximately 3,000 entries representing germplasm from all major winter wheat producing
countries, except China, were evaluated. The average frequency of resistant entries was 15-20%.
Close to 50% of resistant germplasm possessed APR and was susceptible at the seedling stage.
The severities of infection for APR materials were 5-15% higher than lines possessing major
genes. Genetic diversity analysis of a set of resistant genotypes using parentage information
revealed three clusters: USA and USA-derived germplasm with a possible presence of the
Amigo-derived 1A.1R translocation; East European germplasm with strong effect of Bezostaya
in pedigrees, and IWWIP germplasm based on winter x spring crosses. Race Ug99-resistant
germplasm was characterized for resistance to other diseases and agronomic traits, multiplied,
and made available to IWWIP global cooperators for selection and utilization in their programs.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
170
Borlaug Global Rust Initiative • globalrust.org
Studies of wheat species collections for resistance to harmful diseases
E. D. Kovalenko1, T. M. Kolomiets1, M. I. Kiseleva1, L. F. Pankratova1 and H. Bockelman2
1
All Russian Research Institute of Phytopathology, Moscow, Russia; 2USDA-ARS, National
Small Grains Collection, 1691 S. 2700 W., Aberdeen, ID 83210, USA. Email:
kovalenko@vniif.ru; harold.bockelman@ars.usda.gov.
Wheat accessions from NSGC (USA) and WIR (Russia) resistant to leaf rust, Septoria diseases
and powdery mildew were selected in an infection nursery of ARRIP (Central Region of Russia).
The accessions represented 17 subspecies of 6 wheat species, viz. Triticum monococcum
(2n=14), T. turgidum, T. timopheevii, T. ispahanicum (2n=28), T. zhukovskyi and T. aestivum
(2n=42). Resistance depended on ploidy. Diploid wheat T. monococcum accessions were very
resistant to leaf rust and Septoria diseases. Tetraploid wheat accessions T. ispahanicum, T.
turgidum ssp. carthlicum, and T. turgidum ssp. turanicum were resistant to leaf rust, and T.
turgidum ssp. dicoccon, T. turgidum ssp. turgidum, and T. timopheevii ssp. timopheevii to
septoria disease. Hexaploid wheat accessions were characterized by high disease susceptibility.
Four accessions: PI 418584 - WIR 38555 (Georgia), PI 276007 - 103 (Spain), PI 348702 69Z6.886 (Spain), PI 277129 - Subletshchumicum (Switzerland) were the most important due to
their joint resistances to leaf rust, Septoria diseases and powdery mildew.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
171
Borlaug Global Rust Initiative • globalrust.org
Selection of wheat cultivars partially resistant to leaf rust and Septoria diseases
T. M. Kolomiets1, E. D. Kovalenko1, M. I. Kiseleva1, L. F. Pankratova1 and H. Bockelman2
All Russian Research Institute of Phytopathology, Moscow, Russia; 2USDA-ARS, National
Small Grains Collection, 1691 S. 2700 W., Aberdeen, ID 83210, USA. Email:
kolomiets@vniif.ru; harold.bockelman@ars.usda.gov
1
Wheat cultivars partially resistant to leaf rust and Septorias were selected by study of the
following components: area under the disease progress curve (in infection nurseries), latent
period, size and quantity of pustules and spots (in the laboratory, growth cabinet or greenhouse).
Wheat accessions CIGM90.845, BW27720, BW27725, BW27830, BW27832, BW27964,
BW28091, BW28035 (Mexico) from NSGC (USA), Hubara 1 (Syria), Gerak 1, Saratovskaya 74
(Russia) from the WIR collection, and Omskaya 20, Omskaya 37, Omskaya 39, Lutestcens 4140
from the Siberian ARI were characterized by partial resistance to leaf rust. Partial resistance to
SNB in 7 wheat cultivars, Cambridge Rivet (UK), Anderson, (USA), PI 94743 (290) (Russia),
RAC 610, RAC 569, K-20 (South Africa) and SWS "A" №80 (Sweden), was caused by long
latent period and small infectious spots. in 6 accessions: 69Z6.886 (Spain), Owens (USA), Trizo,
Voronezhskaya 10, Lyra 98, and Legenda (Russia) it was caused by long latent period, and
G2697 (Iraq), WIR 38555 (Georgia), PI 290518 (Hungary), Warigal (Australia), KS96WGRC40
(USA) and Enita (Russia) had smaller sized infectious spots than the susceptible check
Priokskaya 1. Partial resistance to STB present in Azteca and Alondra (Mexico) was due to long
latent period, in Piamontes (Argentina) due to small infectious spots, and in Galina (Russia) due
to long latent period and fine infectious spots.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
172
Borlaug Global Rust Initiative • globalrust.org
Phenotyping and genotyping of “Arsenal” accessions for resistance to Puccinia species
I. F. Lapochkina1, A. I. Morgunov2 and G. Woldeab3
1
Moscow Agriculture Research Institute “Nemchinovka”, Moscow Region, Russia; 2CIMMYT,
P.K. 39 Emek 06511 Ankara, Turkey; 3Plant Protection Research Center, Ambo, Ethiopia.
Email: inna-lapochkina@yandex.ru
The “Arsenal” collection comprises about 145 accessions of spring wheat and 150 accessions of
winter wheat derivatives of hybrids involving various species, including Aegilops speltoides, Ae.
triuncialis, Triticum kiharae and Secale cereale. About 40% of spring genotypes are resistant to
leaf rust (severities
Proceedings
Borlaug Global Rust Initiative
2012 Technical Workshop
September 1–4
Beijing, China
Poster Abstracts
Theme 4: Breeding for Rust Resistance
Edited by Robert McIntosh
bgri@cornell.edu
www.globalrust.org
ISBN: 13: 978-0-615-70429-6
© 2012 individual authors and the Borlaug Global Rust Initiative.
Do not reprint without express permission from principal author.
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Proceedings
BRGI 2011 Technical Workshop
Table of contents
Poster Abstracts
Breeding for Rust Resistance
Initiation of genomic recurrent selection for slow-rusting adult plant resistance to
stem rust in wheat
J. Rutkoski, R. P. Singh, J. Huerta-Espino, S. Bhavani and M. E. Sorrells .............................................................. 167
Identification of high yielding, drought tolerant and stripe rust resistant
facultative/winter wheat genotypes at ICARDA
W. Tadesse and S. Rajaram ................................................................................................................................................ 168
Stripe rust and leaf rust responses of a double haploid wheat population derived
from Ducula-4/*Brookton
M. S. Saharan, G. Singh, R. Tiwari, T. L. Setter, V. Panwar and I. Sharma ............................................................. 169
Global variation of winter wheat in response to Pst race Ug99
A. Morgounov, B. Akin, Y. Kaya, M. Keser, L. X. Yu, Y. Jin, S. Martynov, Z. Mert, M. Sorrels
and R. Wanyera ...................................................................................................................................................................... 170
Studies of wheat species collections for resistance to harmful diseases
E. D. Kovalenko, T. M. Kolomiets, M. I. Kiseleva, L. F. Pankratova and H. Bockelman ..................................... 171
Selection of wheat cultivars partially resistant to leaf rust and Septoria diseases
T. M. Kolomiets, E. D. Kovalenko, M. I. Kiseleva, L. F. Pankratova and H. Bockelman ..................................... 172
Phenotyping and genotyping of “Arsenal” accessions for resistance to Puccinia species
I. F. Lapochkina, A. I. Morgunov and G. Woldeab....................................................................................................... 173
Resistance to stripe rust in promising lines and bread wheat cultivars
M. Khodarahmi, A. Omrani and F. Afshari ..................................................................................................................... 174
Screening for stem rust resistance (race Ug99) in East Africa
S. Bhavani, P. Njau, R. Wanyera, A. Badebo and B. Girma ........................................................................................ 175
Developing single seed descent populations for combining biotic and abiotic stress
tolerances in bread wheat
G. Singh, M. S. Saharan, T. L. Setter, M. K. Singh and I. Sharma ............................................................................. 176
Evaluation of stem rust resistant wheat lines for commercial production in Kenya
P. Njau, L. Karani, S. Bhavani, R. Wanyera, J. Huerta-Espino and R. P. Singh ..................................................... 177
The importance of yellow (stripe) rust on wheat in Algeria and release of varieties
resistant to yellow rust and stem rust
A. Benbelkacem, N. Derbal and C. Djenadi .................................................................................................................. 178
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Resistance to threatening of Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici
races in Moroccan bread wheat cultivars and landraces
A. Ramdani, H. Ouabbou, S. Bhavani, K. Nazari, R. Wanyera, S. Lhaloui, F. Abbad-Andaloussi,
N. Nsarellah, S. Bennani, J. Haddouri and S. M.Udupa ............................................................................................. 179
Seedling and adult-plant responses of Iranian wheat genotypes to Sr31-virulent
Pgt races
F. Afshari, M. Aghaee, K. Nazari, M. Patpour, M. Esmaeilzadeh, G. Najafian, A. Yazdansepas, M.
Vahabzadeh, S. Mahfouzi, M. Khodarahmei, A. Amini, M. Mohammadei, A. R. Mohammadi,
M. R. Jalal Kamali, R. Wanyera and S. Bhavani ............................................................................................................. 180
Parsi, Sivand and Sirvan: Three new rust resistant wheat varieties for irrigated
temperate areas of Iran
G. Najafian, H. Amin, M. Dadaein, E. Pazhoumand, A. R. Nikzad, Sh. Sarikhani, R. Nikooseresht,
A. Jafarnezhad, A. Ghandi, D. Afuni, M. Khodarahmi, Gh. H. Ahmadi, A. Amini, H. Abdi, J. Hassanpour,
B. Shabanzadeh, A. Z. Faizabadi, F. Afshari, A. Malihipour, A. Zakeri, M. Yassaei, S. Rajaei,
M. Atahossaini, R. Aghnoum and S. M. T. Tabatabaei ............................................................................................... 181
Breeding for resistance to Pgt race Ug99 in Kazakhstan by application of molecular
markers
A. Kokhmetova, F. Ogbonnaya, M. Atishova, A. Morgounov and Sh. Rsaliev ................................................... 182
Breeding rust resistant wheat in Kyrgyzstan
M. Dzhunusova, A. Morgounov and A. Yahyaoui .................................................................................................... 183
Identification combined resistance to stripe rust and stem rust in Uzbek wheat
germplasm
Z. M. Ziyaev, R. C. Sharma, A. I. Morgounov, A. A. Amanov and Z. F. Ziyadullaev ........................................... 184
Agronomic performance of winter wheat genotypes resistant to Pgt race Ug99 in
Kenya and stripe rust in Uzbekistan
S. Islomov, R. C. Sharma, Z. Ziyadullaev, Z. Ziyaev and Z. Khalikulov ................................................................. 185
Stripe rust responses of wheat varieties released in Azerbaijan
M. Ahmadov .......................................................................................................................................................................... 186
Stem rust responses of Azerbaijani bread and durum wheat accessions
M. Abbasov, R. Bowden, J. Raupp, S. Sehgal, S. Babayeva and B. Gill ................................................................. 187
Reactions of Turkish wheat cultivars and breeding lines to Pgt race Ug99: 2006 - 2011
Z. Mert, K. Akan, L. Çetin, R. Wanyera, S. Bhavani, D. Singh and F. Düşünceli .................................................. 188
Winter bread wheat breeding studies in the transitional rainfed zone of Turkey
S. Belen, N. Bolat, M. Çakmak, A. Yorgancilar and A. T. Kilinç ................................................................................. 189
Wheat rust virulence in southern Russia
G. V. Volkova, O. F. Vaganova, E. V. Sinyak and Y. V. Shumilov .............................................................................. 190
Evaluation of stem rust responses in a spring wheat collection and lines with Sr genes
in the southern forest-steppe zone of Western Siberia
V. P. Shamanin, A. I. Morgounov, M. A. Levshunov, Y. I. Zelenskiy, Sh. Rsaliev, I. V. Pototskaya
and S. L. Petukhovskiy ......................................................................................................................................................... 191
Field responses of near-isogenic lines and wheat varieties to leaf rust in Bangladesh
K. Mustarin, M. M. A. Reza, M. S. Hossain, M. A. Hakim, P. K. Malaker, B. K. Goswami, M. M. Rahman,
N. C. D. Barma, M. M. Rahman, F. Ahmed and R. Islam ............................................................................................ 192
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative 2012 Technical Workshop Proceedings • globalrust.org
Evaluation of wheat lines for resistance to stripe (yellow) rust in mid-hills of Nepal
S. Adhikari, S. M. Shrestha, G. B. Khatri-Chhetri, S. Sharma and R. S. Poudel .................................................... 193
Stem rust resistance in wheat lines from Bangladesh and Nepal
Z. A. Pretorius, R. Prins, P. K. Malaker, N. C. D. Barma, M. A. Hakim, D. Thapa, R. F. Park and G. L. Cisar ... 194
Elite wheat germplasm with resistance to stripe rust and stem rust for the Indian
Northern Hills Zone
V. Rana, S. C. Bhardwaj and S. K. Rana ............................................................................................................................ 195
The National Coordinated Wheat Program in Pakistan
M. Y. Mujahid, M. A. Khan H. Makhdoom, A. Bari, M. Tila, H. Manzoor, M. Tariq, A. Arian, A. Muneer,
A. Rattu, J. I. Mirza, K. Shafiq and R. Ward ..................................................................................................................... 196
Resistant wheat varieties can mitigate the Pgt race Ug99 threat to food security
A. K. Joshi, M. Azab, M. Mosaad, M. Moselhy, R. Sharma, S. Gelalcha, G. Bedada, M. R. Bhatta,
N. R. Gautam, S. R. Updahyay, N. C. D. Barma, D. B. Pandit, A. Hakim, P. K. Malaker, M. E. Haque,
T. P. Tiwari, A. Majid, M. Y. Mujahid, Z. Bishaw, T. Payne, R. P. Singh and H. J. Braun ..................................... 197
Assessment of partial resistance to stripe rust in elite wheat lines*
S. A. Safavi and F. Afshari .................................................................................................................................................... 198
Effect of stripe (yellow) rust on yield components of barley cultivars with race-specific
and slow rusting resistances*
S. A. Safavi, A. B. Ahari, F. Afshari and M. Arzanlou .................................................................................................... 199
Slow stripe rusting resistance in Iranian barley*
S. A. Safavi, A. B. Ahari, F. Afshari and M. Arzanlou .................................................................................................... 200
Effect of temperature on resistance to stripe (yellow) rust on wheat*
R. Bryant, C. Uauy, S. Dorling, L. Boyd and C. Ridout ................................................................................................. 201
Ethiopian farmers’ preferences for attributes of bread wheat: A gender-responsive
conjoint analysis*
K. Nelson, S. Davidson, R. Nelson, Y. Chiche, L. Sperling, S. Hunduma and S. Abebe .................................... 202
Combining resistance to leaf rust and terminal heat tolerance in wheat for the Eastern
Gangetic Plains of India*
C. Tiwari, B. Arun, V. K. Mishra, R. Dhari, U. Kumar, H. Wallwork and A. K. Joshi .............................................. 203
Evaluation of barley germplasm for seedling and adult plant responses (APR) to Pgt
race Ug99*
M. Wamalwa, J. Owuoche, R. Wanyera and S. Bhavani ............................................................................................ 204
Evaluation of Nepalese wheat genotypes for seedling and adult plant resistance to
leaf rust*
S. Baidya, S.C. Bhardwaj, M. Prashar, H. K. Manandhar, S. Sharma, S. K. Shrestha and A. K. Joshi .............. 205
Breeding for resistance to Mycosphaerella graminicola in wheat
S. M. Tabib Ghaffary, J. D. Faris, T. L. Friesen, O. Robert, V. Laurent, P. Lonnet, E. Margalé,
T. A. J. van der Lee, R. G. F. Visser and G. H. J. Kema .................................................................................................. 206
* Abstract submitted to BGRI 2012 Competitive Graduate Student Research Symposium
Borlaug Global Rust Initiative • globalrust.org
Initiation of genomic recurrent selection for slow-rusting adult plant resistance to stem rust
in wheat
J. Rutkoski1, R. P. Singh2, J. Huerta-Espino3, S. Bhavani4 and M. E. Sorrells1
1
Department of Plant Breeding and Genetics, 240 Emerson Hall, Cornell University, Ithaca, NY
14853, USA; 2CIMMYT, Apdo. Postal 6-641, 06600 El Batan, Mexico; 3Campo Experimental,
Valle de México INIFAP, Apdo. Postal 10, 56230 Chapingo, Edo de México, Mexico;
4
CIMMYT, ICRAF House, United Nations Avenue, Gigiri, Village Market-00621, Nairobi,
Kenya. Email: rutkoski.jessica@gmail.com
Genomic selection (GS) is a new breeding technology that promises to increase the rate of
genetic gain for quantitative traits. With GS, a prediction model for the trait(s) of interest is
developed using a relevant population with existing phenotypic and genotypic data. The
prediction model is then applied to a new set of breeding lines which have been genotyped in
order to estimate the breeding values. Based on the predicted breeding values, lines can be
selected for advancement or crossing to initiate the next selection cycle. This allows selection to
occur before phenotypic information for the lines can be generated, therefore enabling faster
selection cycles. In order to empirically evaluate the efficiency of GS relative to phenotypic
selection (PS) for slow-rusting adult plant stem rust resistance (APR), two parallel recurrent
selection (RS) schemes are underway, and are currently at the first selection cycle stage.
Selections are based on phenotypes in the first scheme (RS-PS), and on genotypes and predicted
breeding values in the second scheme (RS-GS). Based on cross-validation using the model
training population, we expect the selection accuracy with GS to be 0.6. Based on phenotypic
data from the same germplasm evaluated across four growing seasons, we expect the selection
accuracy with PS to be 0.78. Because the minimum selection cycle duration is 8 months with GS
and 12 months with PS, considering our estimates of selection accuracy we expect to achieve
1.15 times more genetic gain per unit of time with GS compared to PS.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
167
Borlaug Global Rust Initiative • globalrust.org
Identification of high yielding, drought tolerant and stripe rust resistant facultative/winter
wheat genotypes at ICARDA
W. Tadesse and S. Rajaram
Wheat breeding, ICARDA, P.O. Box 5466, Aleppo, Syria. E-mail: w.tadesse@cgiar.org
Stripe (yellow) rust and drought are the principal yield limiting factors for wheat production in
the CWANA region. ICARDA’s germplasm development approach is to identify genotypes with
high yield potential, high water use efficiency and resistance to stripe rust. Our aim is to
minimize and maximize yield gains during drought and good seasons, respectively. Toward these
objectives we make more than 500 crosses annually, and evaluate the segregating populations
using a modified pedigree/selected bulk approach in Pst-inoculated irrigated nurseries.
Preliminary and advanced yield trials are evaluated under both irrigated and rain-fed conditions.
With this approach we have identified stripe rust resistant genotypes (5-10% severity) which
yield as high as 6.5 and 2.5 t/ha under irrigated (450 mm) and rain-fed (232mm) conditions,
respectively. These genotypes combine high yield potential, drought tolerance, stripe rust
resistance and good grain quality. Some of the genotypes are candidates for release in some
countries.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
168
Borlaug Global Rust Initiative • globalrust.org
Stripe rust and leaf rust responses of a double haploid wheat population derived from
Ducula-4/*Brookton
M. S. Saharan1, G. Singh1, T. L. Setter2, V. Panwar1 and I. Sharma1
1
Directorate of Wheat Research, Karnal, Haryana, India; 2Department of Agriculture and Food,
South Perth, WA 6151, Australia. Email: mssaharan7@yahoo.co.in
Rusts are a threat to sustainable food production. In India leaf rust is a regular occurrence, but
stripe rust can be more damaging even though it is less widespread. New sources of resistance
are required to combat the regular emergence of new races. In the present study, 155 double
haploid lines from Ducula-4/2*Brookton were screened against prevalent races of Pst (78S84,
46S119) and Pt (12-2, 77-2, 77-5, 104-2) at Karnal during 2009 - 2011. Lines were categorized
by Area Under the Disease Progress Curve (AUDPC) values calculated from the rust severities
recorded at regular intervals. Among 155 lines, 32 (group I) were stripe rust-free whereas other
lines gave AUDPC values of 1-100 (group-II, 7 lines), 101-200 (group-III, 21 lines) and 201-500
(group-IV, 38 lines); the remaining lines gave higher values. For leaf rust, 20 lines were rust-free
and 32, 9 and 52 lines were placed in groups II, III and IV, respectively. Group II - IV lines for
both diseases were considered to be slow rusting as their AUDPC values were less than 20% of
the checks. Such slow rusting lines can be used as parents for incorporating slow rusting genes
into breeding populations.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
169
Borlaug Global Rust Initiative • globalrust.org
Global variation of winter wheat in response to Pst race Ug99
A. Morgounov1, B. Akin1, Y. Kaya2, M. Keser3, L. X. Yu4, Y. Jin5, S. Martynov6, Z. Mert7, M.
Sorrels4 and R. Wanyera8
1
CIMMYT, P.O. Box 37, Emek, Ankara 06511, Turkey; 2International Agricultural Research
Institute, P.O. Box125, Karatay, Konya, Turkey; 3ICARDA, P.O. Box 37, Emek, Ankara 06511,
Turkey; 4Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853,
USA; 5USDA-ARS Cereal Disease Laboratory, 1551 Lindig Street, University of Minnesota, St.
Paul, MN 55108, USA; 6Vavilov Institute, 44 B. Morskaya St., St. Petersburg, Russia; 7Central
Field Crop Research Institute, Sehit Cem Ersever, Caddesi No:9-11, Ankara, Turkey; 8Kenya
Agricultural Research Institute, P.O. Private Bag 20107, Njoro, Kenya. Email:
a.morgounov@cgiar.org
The International Winter Wheat Improvement Program (www.iwwip.org) is was established as a
joint project by the Ministry of Food, Agriculture and Animal Husbandry of Turkey, CIMMYT,
and ICARDA in 1986. The objective of the program is to develop facultative and winter wheat
germplasm for the Central and West Asia region. IWWIP also facilitates global germplasm
exchange of winter wheat by assembling lines from various sources, and evaluating and
distributing selected entries through its system of international nurseries. Resistance to rusts is a
high priority along with adaptation and grain quality. Due to the obvious importance of Pst race
Ug99, evaluation of germplasm for resistance in Kenya started in 2008. From 2008 to 2011,
approximately 3,000 entries representing germplasm from all major winter wheat producing
countries, except China, were evaluated. The average frequency of resistant entries was 15-20%.
Close to 50% of resistant germplasm possessed APR and was susceptible at the seedling stage.
The severities of infection for APR materials were 5-15% higher than lines possessing major
genes. Genetic diversity analysis of a set of resistant genotypes using parentage information
revealed three clusters: USA and USA-derived germplasm with a possible presence of the
Amigo-derived 1A.1R translocation; East European germplasm with strong effect of Bezostaya
in pedigrees, and IWWIP germplasm based on winter x spring crosses. Race Ug99-resistant
germplasm was characterized for resistance to other diseases and agronomic traits, multiplied,
and made available to IWWIP global cooperators for selection and utilization in their programs.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
170
Borlaug Global Rust Initiative • globalrust.org
Studies of wheat species collections for resistance to harmful diseases
E. D. Kovalenko1, T. M. Kolomiets1, M. I. Kiseleva1, L. F. Pankratova1 and H. Bockelman2
1
All Russian Research Institute of Phytopathology, Moscow, Russia; 2USDA-ARS, National
Small Grains Collection, 1691 S. 2700 W., Aberdeen, ID 83210, USA. Email:
kovalenko@vniif.ru; harold.bockelman@ars.usda.gov.
Wheat accessions from NSGC (USA) and WIR (Russia) resistant to leaf rust, Septoria diseases
and powdery mildew were selected in an infection nursery of ARRIP (Central Region of Russia).
The accessions represented 17 subspecies of 6 wheat species, viz. Triticum monococcum
(2n=14), T. turgidum, T. timopheevii, T. ispahanicum (2n=28), T. zhukovskyi and T. aestivum
(2n=42). Resistance depended on ploidy. Diploid wheat T. monococcum accessions were very
resistant to leaf rust and Septoria diseases. Tetraploid wheat accessions T. ispahanicum, T.
turgidum ssp. carthlicum, and T. turgidum ssp. turanicum were resistant to leaf rust, and T.
turgidum ssp. dicoccon, T. turgidum ssp. turgidum, and T. timopheevii ssp. timopheevii to
septoria disease. Hexaploid wheat accessions were characterized by high disease susceptibility.
Four accessions: PI 418584 - WIR 38555 (Georgia), PI 276007 - 103 (Spain), PI 348702 69Z6.886 (Spain), PI 277129 - Subletshchumicum (Switzerland) were the most important due to
their joint resistances to leaf rust, Septoria diseases and powdery mildew.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
171
Borlaug Global Rust Initiative • globalrust.org
Selection of wheat cultivars partially resistant to leaf rust and Septoria diseases
T. M. Kolomiets1, E. D. Kovalenko1, M. I. Kiseleva1, L. F. Pankratova1 and H. Bockelman2
All Russian Research Institute of Phytopathology, Moscow, Russia; 2USDA-ARS, National
Small Grains Collection, 1691 S. 2700 W., Aberdeen, ID 83210, USA. Email:
kolomiets@vniif.ru; harold.bockelman@ars.usda.gov
1
Wheat cultivars partially resistant to leaf rust and Septorias were selected by study of the
following components: area under the disease progress curve (in infection nurseries), latent
period, size and quantity of pustules and spots (in the laboratory, growth cabinet or greenhouse).
Wheat accessions CIGM90.845, BW27720, BW27725, BW27830, BW27832, BW27964,
BW28091, BW28035 (Mexico) from NSGC (USA), Hubara 1 (Syria), Gerak 1, Saratovskaya 74
(Russia) from the WIR collection, and Omskaya 20, Omskaya 37, Omskaya 39, Lutestcens 4140
from the Siberian ARI were characterized by partial resistance to leaf rust. Partial resistance to
SNB in 7 wheat cultivars, Cambridge Rivet (UK), Anderson, (USA), PI 94743 (290) (Russia),
RAC 610, RAC 569, K-20 (South Africa) and SWS "A" №80 (Sweden), was caused by long
latent period and small infectious spots. in 6 accessions: 69Z6.886 (Spain), Owens (USA), Trizo,
Voronezhskaya 10, Lyra 98, and Legenda (Russia) it was caused by long latent period, and
G2697 (Iraq), WIR 38555 (Georgia), PI 290518 (Hungary), Warigal (Australia), KS96WGRC40
(USA) and Enita (Russia) had smaller sized infectious spots than the susceptible check
Priokskaya 1. Partial resistance to STB present in Azteca and Alondra (Mexico) was due to long
latent period, in Piamontes (Argentina) due to small infectious spots, and in Galina (Russia) due
to long latent period and fine infectious spots.
Proceedings, BGRI 2012 Technical Workshop, September 1-4, Beijing, China
ISBN: 13: 978-0-615-70429-6
172
Borlaug Global Rust Initiative • globalrust.org
Phenotyping and genotyping of “Arsenal” accessions for resistance to Puccinia species
I. F. Lapochkina1, A. I. Morgunov2 and G. Woldeab3
1
Moscow Agriculture Research Institute “Nemchinovka”, Moscow Region, Russia; 2CIMMYT,
P.K. 39 Emek 06511 Ankara, Turkey; 3Plant Protection Research Center, Ambo, Ethiopia.
Email: inna-lapochkina@yandex.ru
The “Arsenal” collection comprises about 145 accessions of spring wheat and 150 accessions of
winter wheat derivatives of hybrids involving various species, including Aegilops speltoides, Ae.
triuncialis, Triticum kiharae and Secale cereale. About 40% of spring genotypes are resistant to
leaf rust (severities