I
Kompur
-
Dramoga.
2007
Fat
Fat
was
according to The
was 3
than with
paper
in
the up part while part was
free cotton
prepared fat
cup
which
the
weight
and attached with tube.
The
tube
was
put extractor of
tube, then
sprayed
with dissolved
benzene,
ex
traction
was
done 6 hours
in
40
After the
had fished, the
tube brought out. The fat
who
is
in fat cup, was until all
of
the
fat steamed.
then fat cup
was
dried
in
oven
at for
3-5 hours.
The cold fat cup
was
in desiccators constant
Fat
=
Carbohydrate
level was
analyzed
to
Winamo, 1992 Carbohydrate level was reached
by
the difference of
4
level, protein, fat ask.
is; Carbohydrate
=
100
+
+
+Ask
2. Amino
acid level
was
analyzed according
The acid
by using The
of
HPLC used
in
this research is water with the
of
acid based
on
acid base, it
used
tag
acids water color.
The used
as
phase
and
t
xylena as quite phase. The completing
a.
Acid
hydrolysis
The
at 0.25
was
measured
closed
tube
reaction
added
5
and by
N2
gas
closed. After
that,
the
was
put
at
for hours,
liquid sample filtered
by using fitter paper.
Drying
liquid, hydrolysis
process, was
at
1
to at
30
I drying liquid methanol:
Na-acetate :
acetate
After that, by vacuum
pressure 50
THP
dun
Perikonon I
-
2007
liquid
.
7 :
1 : 1.
The added
at
30 and then
it
was
ignored about 20 minutes, dried
by
pump, 50
pressure. After that,
dried
was diluted by 200 diluter
Na-acetate
and
satnple liquid which
is
ready to be
analyzed.
Acid analysis by HPLC
lhe condition of
occurred is:
1 .
Color
:
2. Color
tag 3.9
x
3. Water speed
: 1.5
4. Pressure
.
3000
psi
5. :
Gradient
:
-
Asetonitril 60 Buffer
N
7.
Detector UV
The length of wave
:
254 percentage of amino acid in
100 grams
deep be measured by
The o f
area standard
..... 6
BMA
the
weight of amino acid molecule
3.
Steroid
Hormone Test
Steroid extraction
was
done
based
a reported by Touchtone
and Kasparov 1
970
referred by Riris of deep sea
at
20 grams. by blender
was
by of cold
and then saved for
24 hours in cold at
40
C, centrifuged
for 10 minutes. The
deposit
was
frotn the liquid phase. The
liquid
phase
was
water heater at The obtained residue
2 times
in
etil acetate,
and water by using separation
so
at extraction
liquid
solution down layer
i s
up
layer is etil
acetate
was
in water heater
at 4
until dry.
extract
was
used to
was done by Liebennan
Test-Addition
some acetate anhydrate acid and
0,5
ml
to
a
extract
of deep then stirred
i t
a
drop of sulfate. The green color
showed
extract
contained steroid
958
referred 1 994.
dan
I
-
17-18 Juli 2007
111.
AND DISCUSSION
1 . Test
of
pmxitnate
analysis of deep
sea
shows that
there
are
of each
sea fish.
It depends
on
at
species,
age
the
at area
1969
by
Septarina 999.
Table
The
of analysis
Explanations.
:
A h A 7
A 3
:
,
A 4
:
crassipinus 10
:
A 5 I
:
Protein level
shows
that
o f
deep
sea
fishs
protein
varies
from
to 24.8 Based
Olcott cited
by
1998,
deep fishs
is
in high
level at
protein and low it comparing
with
pelagic
fish,
the
of sea
fishs
is higher
at
pelagic fish,
shows
in Table 2
that
crassipinus
contain
the highest at protein of 27.4
with other researched
sea
fish.
THP
29
don
I
-
7-18
Explanations.
: :
tenuis A3 :
A4
:
: :
: A10 :
sp I
:
.
of Deep Sea protein Table
pelagic fish
RDD
: a.
Hardiansyah
and
D
1994
b.
c . 2000
of
Protein
Fish
Content
20.00
16.00 19.90
