Uji biopotensi antibodi monoklonal anti pregnant mare serum gonadotropin pada sapi perah
Vol. 5. No. 3
Hayati, September 1998, hlm. 73-78
ISSN 0854-8587
Uji Biopotensi Antibodi Monoklonal Anti Pregnant Mare Serum
Gonadotropin pada Sapi Perah
(Biopotency Test of Monoclonal Antibody Anti Pregnant Mare Serum
Gonadotropin in Dairy Cattle)
IMAN
SUPRIATNAI*,
TUTY L. YUSUF', BAMBANG PURWANTARA', G O Z A L I MOEKT12,
DAN LIES PAREDE HERNOMOADI'
'Bagian Reproduksi dun Kebidanan FKH IPB, Jdan Lodaya II, Bogor 16151
'Balai Penelitian VeterinerDepartemen Pertanian RI, Jalan R.E. Martadinata No. 30, Bogor 16123
Diterima 05 Mei 1998iDisetujui18 Agustus 1998
The experiment was conducted to determine whether MoAb anti pregnant mare serum gonadotropin (PMSG)
(PHB I1 Product) possess neutralizing capacity and to determine an effective neutralizing biopotency against commercial
PMSG used in dairy heifer cattle. Twenty five Fries Holland (FH) heifer donors were randomly divided into 5 groups.
Group I as a negative control was superovulated with 2000 IU PMSG intramuscularly (i.m.) at 03:OO PM. Donors in
group 11, 111, IV, and V received the same treatment as those in group I, but were also administered with MoAb
intravenously (i.v.) 30 minutes after PMSG injection. Group I1 as a positive control received 3.3 ml commercial MoAb
(Neutra PMSG), whereas groups 111, IV, and V received 0.5 ml; 1.0 ml and 2.0 ml MoAb (PHB I1 Product), respectively.
Biopotency test in dairy heifer donors, showed that 2000 IU PMSC used for superovulation was found to be not
neutralized by 0.5 ml in group I11 and 1.0 ml of MoAb (PHB I1 Product) in group IV. Superovulation reaction observed
were not signifiantly different ( M . 0 5 ) from that observed in group I donors (untreated with MoAb). However when
the dose was increased to 2.0 ml, superovulation reaction in treated donors group V were significantly different (P
Hayati, September 1998, hlm. 73-78
ISSN 0854-8587
Uji Biopotensi Antibodi Monoklonal Anti Pregnant Mare Serum
Gonadotropin pada Sapi Perah
(Biopotency Test of Monoclonal Antibody Anti Pregnant Mare Serum
Gonadotropin in Dairy Cattle)
IMAN
SUPRIATNAI*,
TUTY L. YUSUF', BAMBANG PURWANTARA', G O Z A L I MOEKT12,
DAN LIES PAREDE HERNOMOADI'
'Bagian Reproduksi dun Kebidanan FKH IPB, Jdan Lodaya II, Bogor 16151
'Balai Penelitian VeterinerDepartemen Pertanian RI, Jalan R.E. Martadinata No. 30, Bogor 16123
Diterima 05 Mei 1998iDisetujui18 Agustus 1998
The experiment was conducted to determine whether MoAb anti pregnant mare serum gonadotropin (PMSG)
(PHB I1 Product) possess neutralizing capacity and to determine an effective neutralizing biopotency against commercial
PMSG used in dairy heifer cattle. Twenty five Fries Holland (FH) heifer donors were randomly divided into 5 groups.
Group I as a negative control was superovulated with 2000 IU PMSG intramuscularly (i.m.) at 03:OO PM. Donors in
group 11, 111, IV, and V received the same treatment as those in group I, but were also administered with MoAb
intravenously (i.v.) 30 minutes after PMSG injection. Group I1 as a positive control received 3.3 ml commercial MoAb
(Neutra PMSG), whereas groups 111, IV, and V received 0.5 ml; 1.0 ml and 2.0 ml MoAb (PHB I1 Product), respectively.
Biopotency test in dairy heifer donors, showed that 2000 IU PMSC used for superovulation was found to be not
neutralized by 0.5 ml in group I11 and 1.0 ml of MoAb (PHB I1 Product) in group IV. Superovulation reaction observed
were not signifiantly different ( M . 0 5 ) from that observed in group I donors (untreated with MoAb). However when
the dose was increased to 2.0 ml, superovulation reaction in treated donors group V were significantly different (P