The role of low dose nicotine on the profile of blood glucose and insulin, and alteration of pancreas and liver tissues of obese cynomolgus monkey (Macaca fascicularis)
PERAN NIKOTIN DOSIS RENDAH TERHADAP
PROFIL GLUKOSA DARAH DAN INSULIN
SERTA PERUBAHAN JARINGAN PANKREAS DAN HATI
MONYET EKOR PANJANG (Macaca fascicularis) OBES
CHUSNUL CHOLIQ
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2012
PERNYATAAN MENGENAI DISERTASI DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa disertasi berjudul Peran Nikotin Dosis
Rendah Terhadap Kadar Glukosa Darah dan Insulin serta Perubahan
Jaringan Pankreas Dan Hati Monyet Ekor Panjang (Macaca fascicularis)
Obes adalah karya saya sendiri dengan arahan komisi pembimbing, dan belum
diajukan dalam bentuk apapun kepada perguruan tinggi manapun. Sumber
informasi yang berasal atau dikutip dari karya yang diterbitkan maupun yang
tidak diterbitkan dari penulis lain telah disebutkan dalam tulisan dan dicantumkan
dalam Daftar Pustaka di bagian akhir tulisan ini.
Bogor, Juli 2012
Chusnul Choliq
P063070011
ABSTRACT
CHUSNUL CHOLIQ. The Role of Low Dose Nicotine on The Profile of Blood
Glucose and Insulin, and Alteration of Pancreas and Liver Tissues of Obese
Cynomolgus Monkey (Macaca fascicularis). Under supervison DONDIN
SAJUTHI, IRMA HERAWATI SUPARTO, DEWI APRI ASTUTI and RETNO
WULANSARI.
Study of low dose nicotine intervention and its effect on blood and cellular
level of obese cynomolgus monkeys has never been reported. The aims of this
study were to evaluate the role of low dose nicotine on the profile of blood
glucose, insulin level and cellular level alteration of pancreatic and hepatic tissue
of obese cynomolgus monkey. Fourteen adult (aged 6–8 years) males cynomolgus
monkeys grouped based on their Body Mass Index (BMI) into preobese
(BMI=23.65–25.00) and obese (BMI ≥ 26.00) were used. Animals were grouped
into four groups subsequently preobese with nicotine (pOb+), obese with nicotine
(Ob+), preobese without nicotine (pOb-) and obese without nicotine (Ob-).
Nicotine groups were fed with high fat diet mixed with nicotine dose 0.75 mg/kg
body weight/day for three months and other groups without nicotine were fed
monkey chow as control. Blood samples were collected every month for glucose
analysis and insulin analysis at the end of study before necropsy was performed.
Pancreatic and hepatic tissues were processed histologically and stained with
hematoxylin-eosin and immunohistochemical method. The results showed that
blood glucose significantly decreased (P
PROFIL GLUKOSA DARAH DAN INSULIN
SERTA PERUBAHAN JARINGAN PANKREAS DAN HATI
MONYET EKOR PANJANG (Macaca fascicularis) OBES
CHUSNUL CHOLIQ
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2012
PERNYATAAN MENGENAI DISERTASI DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa disertasi berjudul Peran Nikotin Dosis
Rendah Terhadap Kadar Glukosa Darah dan Insulin serta Perubahan
Jaringan Pankreas Dan Hati Monyet Ekor Panjang (Macaca fascicularis)
Obes adalah karya saya sendiri dengan arahan komisi pembimbing, dan belum
diajukan dalam bentuk apapun kepada perguruan tinggi manapun. Sumber
informasi yang berasal atau dikutip dari karya yang diterbitkan maupun yang
tidak diterbitkan dari penulis lain telah disebutkan dalam tulisan dan dicantumkan
dalam Daftar Pustaka di bagian akhir tulisan ini.
Bogor, Juli 2012
Chusnul Choliq
P063070011
ABSTRACT
CHUSNUL CHOLIQ. The Role of Low Dose Nicotine on The Profile of Blood
Glucose and Insulin, and Alteration of Pancreas and Liver Tissues of Obese
Cynomolgus Monkey (Macaca fascicularis). Under supervison DONDIN
SAJUTHI, IRMA HERAWATI SUPARTO, DEWI APRI ASTUTI and RETNO
WULANSARI.
Study of low dose nicotine intervention and its effect on blood and cellular
level of obese cynomolgus monkeys has never been reported. The aims of this
study were to evaluate the role of low dose nicotine on the profile of blood
glucose, insulin level and cellular level alteration of pancreatic and hepatic tissue
of obese cynomolgus monkey. Fourteen adult (aged 6–8 years) males cynomolgus
monkeys grouped based on their Body Mass Index (BMI) into preobese
(BMI=23.65–25.00) and obese (BMI ≥ 26.00) were used. Animals were grouped
into four groups subsequently preobese with nicotine (pOb+), obese with nicotine
(Ob+), preobese without nicotine (pOb-) and obese without nicotine (Ob-).
Nicotine groups were fed with high fat diet mixed with nicotine dose 0.75 mg/kg
body weight/day for three months and other groups without nicotine were fed
monkey chow as control. Blood samples were collected every month for glucose
analysis and insulin analysis at the end of study before necropsy was performed.
Pancreatic and hepatic tissues were processed histologically and stained with
hematoxylin-eosin and immunohistochemical method. The results showed that
blood glucose significantly decreased (P