Identification Of Polymorphism On Simple Sequence Repeats Markers Associated With Brown Planthopper Resistance Genes In Twenty Rice Genotypes And Their Genetic Relationship.
P R O C E E D T NCOB S 2 0 l 3 B O L ' c N , tY O G Y A K A R TIAS E p T E M B E R 2 02 1 2 0 l 3
o-MB-l4
ID:NTIFICATION
OF POLYMORPHISM
ON SIMPLES;QUEI!C: RsrrATS
MARKERSASSOCIATgDWITH BROWITJ
PLANTHOPPERR€SISTANCEGENESIN
TWENTYRICSGE't]OTYPES
AND THEIIl OENETICRELA:IONSHIP
NonoCarsonol',
YessikhaV Barusa,
SanflkaSari5,WinnyD. W darmis,DanarDon02,
YayanSumeka13,
andlvlurdaninqsih
H.Kl
I Slalf L€b oi Plant Biotechno
ogy antt a r e e di ( ] . F a c r r t yo f A g r i c ! t L u r eU. n v e r . u t . r p
s adlr.tl.ranJ.lrnangor.
Bandrng S!medafg 45363 E'ma : icarsono@Lrnpad
. i C l A L r r h orro f . o r r o s p o n d e n c e )
, Stafi,Lab.f Enlono ogy.
F a . L r l t 0y t A g r l c ul u r e ,U i i v e . s r l i s? a d j a d l a r a nJ a Lf r a . j g o r
Ban.iung SLrmedarg.l5363
I Staft, L . r bo JW . o d
S c e f . o . F a c ! 1 yo f A q r c r t r u r e ! f v c r c i t a sp . , i t a d t E r a rJ n t n a n g o /
tsand!ng Sumodang,15363.
I StudenA
l . g r o l e c h n o t s gSyI L ' d yP r o ! f a n r F a . u l t yo f A g f c ! r ! r e U r i i v r y st a s p , r a , a C l a r a.nl . l n a r g o r
A : r a i r n g S ! m e d ar g a t i 3 r j t l
j
StLrdentl./lastcfProgrami n P r f l B r e . r lf g . F a c L r i royf A ! l | l c LriL r r eU. N , ! e , st a s p a . l a . l t r i . i n J a l t i a n q n r
Band!nil Srm0.t..g4536tj
ABSTRACT
Brownp anthopperis one or ihe mosrdeskuctiveinsectpest oi fire in Indonesra
af.r otherAsian
couniries.
Pyramiding
some brownpranthopper
resistance
genesis a va !ab e appfoacfro cfearemore
dLrrable
resisiafceagarmst
the pest rhe obrecliveoflhis srlrciyw3sto i{ientiay
poiynrofphisris
of Bfown
Plafthopper
genes(Bph)on 20 genotypesoi -roe.anCto obtatngenetrcrerrrronshrp
Resistance
among
genotypes
lested rrre experirrenlwas corductedlrom JLrrre
to septembef2012 at GreenHouseand
Laboralory
of PlantAnalysts
and B olechnology,
Facuty ofAgrtcrlture.
Ufiversiiaspadladlafan.
Jatifangor
genoiypeswere analyzed,and two oithern were L_rsed
Tw-eniy
irs cheakvarreles SimpleSequenceRepeat
(SSR)markerswere appliedto Cetecigrrj, Bph| Obph3and Olrph4n--nes.
Fot;morphrctevetswefe
analyzed
by ca.ulat ng plC \polymarphclnfomatronCorter0.The grolrp;Igofricegenorypes
wereoone
Dasedon principal
comlonentsana ysis (pCA)of SSR data.arrclthe gen;tic relationship
basedon the
presenceof8ph geres was est meted us | UPGtt/|t\(.Ltn\'teighted
p ai Graup With Atithrletic rneer) Resu)ts
!l
showedlhatRM3T3.Rl\18072,
Rl,482T3,
RMS9S3,Rl\4586,
and RM589rnafkerswere potyr.ofphrcRic-,
gefo-types
PTB33. DrahSuci.Crbogo,
Ctsanlaf: DtgutChera g tnpar1:l,tnpafj 0, andMemberarbhad
Bph3 Qbph1 Bph.1and Qbpht. t\teanwl )e BphS.AbphS anc:lptt,1,ur.]fes;ppose.t
to Deberonge(,y tR
64,AekSbLrndofg
Balangcadis,R66 anctl\4ekongga
llri mas nri Tukalpenatlrhad AphJ.Obphs,daf
Obpr4 lR 74 had Bprsafa Obph3,and Falma,,!.,)Ith.tt Bph3 andBphz].uPGtvtA
ctusterng resu ted rntvr'o
rnarfcusters.in whch lhe firsl cllste. colrsistedof 2 subclslers prB 33 /as
cosey reraieclwilh
l,4emberano
TukatPeraiLr,
Diqul.DlahSLc andKairnas.Thessn|.af(ersusedrf:ti.isi|r.tywere!foven
arLrF'!rior''rBphqenesardineslmatinggcneir.felalronsipsoirkresenotypes
:r *r b
" 1r J: lW
: : !Fr s: a q
l l ol o: :0 L l o f r o r , o l r e s i s t d . c g c I e s . a s w e t a s l v t e m b e r . I n o T u k : r t p e n a i u . D i g u t , D i a h s L r c t , a
^ar/mas
w r .rt trLircde ' iiFdrj pron ,/ngdofofs tn ricebreedjng.esstanceto brovii plallL|oppef.
Keywords:Bpr!ene.Bfowf ptantlto!per,
cenellcfe ationship,
SSR mafkers
INTRODUCTIOI\
Indonesia
andtheotherAsian
cot]ntries
arecurrenfly
facingproblems
tnsecunng
sup_
pliesof rice.Onereasonof theclecline
produciion
in rice
is thebrownplanthoppers
(BpH,
Nilaparuata
lugensStal.
pests.
BpH
global
is
a
threatbecause
)
notonlyattacK
ngncecrops
n Indonesia,
butalsoattacking
flcecropsln China,Thailand,
Vtetnam,
India,Bangladesh,
l\,4alaysia,
PhiJippines,
Japan,andKorea(Baehaki,2007)
Interesting
phenomenon
in the
interaction
betlveen
thericevarietles
to brownplanthopper
s theappearance
ofbiotvpe,
the
98
r
Llt
Da
:
.to).
t
t...\.
o-MB-l4
ID:NTIFICATION
OF POLYMORPHISM
ON SIMPLES;QUEI!C: RsrrATS
MARKERSASSOCIATgDWITH BROWITJ
PLANTHOPPERR€SISTANCEGENESIN
TWENTYRICSGE't]OTYPES
AND THEIIl OENETICRELA:IONSHIP
NonoCarsonol',
YessikhaV Barusa,
SanflkaSari5,WinnyD. W darmis,DanarDon02,
YayanSumeka13,
andlvlurdaninqsih
H.Kl
I Slalf L€b oi Plant Biotechno
ogy antt a r e e di ( ] . F a c r r t yo f A g r i c ! t L u r eU. n v e r . u t . r p
s adlr.tl.ranJ.lrnangor.
Bandrng S!medafg 45363 E'ma : icarsono@Lrnpad
. i C l A L r r h orro f . o r r o s p o n d e n c e )
, Stafi,Lab.f Enlono ogy.
F a . L r l t 0y t A g r l c ul u r e ,U i i v e . s r l i s? a d j a d l a r a nJ a Lf r a . j g o r
Ban.iung SLrmedarg.l5363
I Staft, L . r bo JW . o d
S c e f . o . F a c ! 1 yo f A q r c r t r u r e ! f v c r c i t a sp . , i t a d t E r a rJ n t n a n g o /
tsand!ng Sumodang,15363.
I StudenA
l . g r o l e c h n o t s gSyI L ' d yP r o ! f a n r F a . u l t yo f A g f c ! r ! r e U r i i v r y st a s p , r a , a C l a r a.nl . l n a r g o r
A : r a i r n g S ! m e d ar g a t i 3 r j t l
j
StLrdentl./lastcfProgrami n P r f l B r e . r lf g . F a c L r i royf A ! l | l c LriL r r eU. N , ! e , st a s p a . l a . l t r i . i n J a l t i a n q n r
Band!nil Srm0.t..g4536tj
ABSTRACT
Brownp anthopperis one or ihe mosrdeskuctiveinsectpest oi fire in Indonesra
af.r otherAsian
couniries.
Pyramiding
some brownpranthopper
resistance
genesis a va !ab e appfoacfro cfearemore
dLrrable
resisiafceagarmst
the pest rhe obrecliveoflhis srlrciyw3sto i{ientiay
poiynrofphisris
of Bfown
Plafthopper
genes(Bph)on 20 genotypesoi -roe.anCto obtatngenetrcrerrrronshrp
Resistance
among
genotypes
lested rrre experirrenlwas corductedlrom JLrrre
to septembef2012 at GreenHouseand
Laboralory
of PlantAnalysts
and B olechnology,
Facuty ofAgrtcrlture.
Ufiversiiaspadladlafan.
Jatifangor
genoiypeswere analyzed,and two oithern were L_rsed
Tw-eniy
irs cheakvarreles SimpleSequenceRepeat
(SSR)markerswere appliedto Cetecigrrj, Bph| Obph3and Olrph4n--nes.
Fot;morphrctevetswefe
analyzed
by ca.ulat ng plC \polymarphclnfomatronCorter0.The grolrp;Igofricegenorypes
wereoone
Dasedon principal
comlonentsana ysis (pCA)of SSR data.arrclthe gen;tic relationship
basedon the
presenceof8ph geres was est meted us | UPGtt/|t\(.Ltn\'teighted
p ai Graup With Atithrletic rneer) Resu)ts
!l
showedlhatRM3T3.Rl\18072,
Rl,482T3,
RMS9S3,Rl\4586,
and RM589rnafkerswere potyr.ofphrcRic-,
gefo-types
PTB33. DrahSuci.Crbogo,
Ctsanlaf: DtgutChera g tnpar1:l,tnpafj 0, andMemberarbhad
Bph3 Qbph1 Bph.1and Qbpht. t\teanwl )e BphS.AbphS anc:lptt,1,ur.]fes;ppose.t
to Deberonge(,y tR
64,AekSbLrndofg
Balangcadis,R66 anctl\4ekongga
llri mas nri Tukalpenatlrhad AphJ.Obphs,daf
Obpr4 lR 74 had Bprsafa Obph3,and Falma,,!.,)Ith.tt Bph3 andBphz].uPGtvtA
ctusterng resu ted rntvr'o
rnarfcusters.in whch lhe firsl cllste. colrsistedof 2 subclslers prB 33 /as
cosey reraieclwilh
l,4emberano
TukatPeraiLr,
Diqul.DlahSLc andKairnas.Thessn|.af(ersusedrf:ti.isi|r.tywere!foven
arLrF'!rior''rBphqenesardineslmatinggcneir.felalronsipsoirkresenotypes
:r *r b
" 1r J: lW
: : !Fr s: a q
l l ol o: :0 L l o f r o r , o l r e s i s t d . c g c I e s . a s w e t a s l v t e m b e r . I n o T u k : r t p e n a i u . D i g u t , D i a h s L r c t , a
^ar/mas
w r .rt trLircde ' iiFdrj pron ,/ngdofofs tn ricebreedjng.esstanceto brovii plallL|oppef.
Keywords:Bpr!ene.Bfowf ptantlto!per,
cenellcfe ationship,
SSR mafkers
INTRODUCTIOI\
Indonesia
andtheotherAsian
cot]ntries
arecurrenfly
facingproblems
tnsecunng
sup_
pliesof rice.Onereasonof theclecline
produciion
in rice
is thebrownplanthoppers
(BpH,
Nilaparuata
lugensStal.
pests.
BpH
global
is
a
threatbecause
)
notonlyattacK
ngncecrops
n Indonesia,
butalsoattacking
flcecropsln China,Thailand,
Vtetnam,
India,Bangladesh,
l\,4alaysia,
PhiJippines,
Japan,andKorea(Baehaki,2007)
Interesting
phenomenon
in the
interaction
betlveen
thericevarietles
to brownplanthopper
s theappearance
ofbiotvpe,
the
98
r
Llt
Da
:
.to).
t
t...\.