The Success of Embryo Transfer in Dairy Cattle Recipient Using Beef Cattle Embryos Repository - UNAIR REPOSITORY
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ELECTRONIC ISSN 2067-2330
CD-ROM ISSN 2284-6964
UNIVERSITATEA DE $TIINJE ACRICOLE
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University of Applied Life Sciences and Environment
11
SCIENTIFIC PAPERS
ANIMAL SCIENCES
LUCRAiu fTIINfiFICE
SERIA ZOOTEHNIE
VOL. 65 (21)
EDITURA " ION IONESCU DE LA BRAD"
,
2016
IA~
COORDINATORS:
Editor-in-chief: Prof.univ.dr. Vasile VINTU, U.S.A.M.V. lasi, ROMANIA
Associate Editor: Prof.univ.dr. Constantin LEONTE, U.S.A.M.V. lasi, ROMANIA
MEMBERS:
-
Prof.univ.dr.
Prof.univ.dr.
Prof.univ.dr.
Prof.univ.dr.
Teodor ROBU- U.S.A.M.V. lasi, ROMANIA
Lucia DRAGHIA- U.S.A.M.V. lasi, ROMANIA
Benone PASARIN- U.S.A.M.V. lasi, ROMANIA
Liviu MIRON- U.S.A.M.V. lasi, ROMANIA
EDITORIAL BOARD OF THE ANIMAL SCIENCE SERIES:
Editor-in-chief: Prof.univ.dr. Benone PASARIN, U.S.A.M.V. lasi, ROMANIA
Associate Editor: Prof.univ.dr. Constantin PASCAL, U.S.A.M.V. Iasi, ROMANIA
EDITORS:
-
Prof.univ.dr. dr. H.C. Liviu AI. MARGHIT~U.S.A.M.V. Cluj-Napoca, ROMANIA
U.S.A.M.V. lai, ROMANIA
Prof.univ.dr. loan VACRU-OPI~
Prof.univ.dr. loan GILCA- U.S.A.M.V. lasi, ROMANIA
Asist.univ.dr. Razvan Mihail RADU-RUSU- U.S.A.M.V. lasi, ROMANIA
Seflucr.dr. Bogdan Vlad AVARVAREI- U.S.A.M.V.Iasi, ROMANIA
Dr. H.C. Yves NYS- L'institut National de Ia Rechearche Agronomique (INRA), FRANCE
Prof.univ.dr. Mohamed A. KENAWI- Minia University, EGYPT
Prof.univ.dr. Otto SZENCI- Szent Istvan University. Budapest- HUNGARY
Prof.univ.dr. Mark RUSSELL- College of Agriculture, Purdue University, Indiana, USA
Prof.univ.dr. Frans;oise PICARD- Universite d'Angers, FRANCE
REVIEWERS:
-
Prof.univ.dr. Victor CRISTEA- Univ. "Dunarea de Jos" GALATI, ROMANIA
Prof.univ.dr. llie VAN- U.S.A.M.V. BUCURESTI, ROMANIA
Prof.univ.dr. Augustin VLAIC- U.S.A.M.V. CLUJ NAPOCA, ROMANIA
Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Prof.univ.dr. Paul Corneliu BOI~TEANUProf.univ.dr. loan Mircea POP- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Prof.univ.dr. Marius Giorgi USTUROI- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
REPUBLIC OF MOLProf.univ.dr. Nicolae EREMIA - Universitatea Agrara de Stat Chi~nau,
DOVA
Prof.univ.dr. Nicolae PACALA- U.S.A.M.V.B. TIMISOARA, ROMANIA
Prof.univ.dr. ~teofil
CREANGA- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Lector- Dr. Hendronoto A.W. LENGKEY- Padjadjaran University, INDONESIA
©2007 UASVM
la~i
I Last update: May, 04, 2016
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
CUPRINS (TABLE OF CONTENTS)
1.
ATTEMPET TO PRODUCE COLD RESISTANCE RABBITS ....................................................
3
T.M. El-Sheikh, T.S. T. Seleem
2.
EVALUATION OF THE REPRODUCTION FUNCTION OF GOATS IN ROMANIA ...........
9
C. Pascal, N. Zaharia
3.
INVESTIGATION OF ZEARALENONE TOLERANCE LIMIT IN THE FEEDSTUFFS FOR
WEANED PIGS........................................................................................................................................
14
lonelia Taranu, M.A. Gras, Gina Cecilia Pistol, Monica Motiu, Daniela Marin,
Mariana Stancu
4.
EFFECT OF GENOTYPE AND NATURAL OR ARTIFICIAL INSEMINATION ON
INDIGENOUS AND ADDAPTED RABBIT PERFORMANCE ..................................................
19
T.M. El-Sheikh, T.S. T. Seleem
5.
EFFECT OF USING RAW MATERIALS RICH IN XANTHOPHYLL$ IN LAYER DIETS
ON EGG QUALITY..................................................................................................................................
25
T.D. Panaite, R.D. Criste, /. Varzaru, M. Cornescu, M. Olteanu
6.
RELATIONSHIP BETWEEN LIPTEN HORMONE CONCENTRATION AND RABBIT
REPRODUCTIVITY ...............................................................................................................................
32
I.A. El-Wardany, N.F.A. Saleh, T.S. T. Seleem, A.M.H. Ahmed
7.
EFFECT OF SPERM CONCENTRATION AND SITE OF INSEMINATION ON
CONCEPTION RATE OF RABBITS ..................................................................................................
38
Rowida M. Rriad, T.S. T. Seleem, KH. El-Kholy, Z.M. Rawash
8.
STUDY ON THE INFLUENCE OF THE INCUBATION EGGS KEEPING POSITION
DURING STORAGE AND THEIR OLDNESS ON HATCHING RESULTS OF THE
BALOTESTI QUAILS POPULATION ...............................................................................................
G. Nica, L. /onitii, Elena Popescu-Mil~an,
9.
44
N. Stanciu
CORELATION BETWEEN THE CONTENT OF HEAVY METALS (Pb, Cd and Cu) IN
THE BEE PRODUCTS AND THE BEES BODY.............................................................................
49
Olesea Gliga, Valentina Cebotari, I. Buzu
10. THE MICROELEMENT MOLYBDENUM (Mo) IN NUTRITIONAL SUPPLEMENT
FOR BEES.................................................................................................................................................
Valentina Cebotari, /. Todera~,
56
A. Gulea, /. Buzu
11. MORPHOLOGICAL STUDY CONCERNING THE REPRODUCTION HORSES FROM
ROMANIAN TRAPA~ BREED FROM JEGALJA STUD ..............................................................
M.G. Doli~,
C.E. Nistor, V. Biicilii, C. $onea
64
12. STUDY OF SOME PRODUCTIVE AND REPRODUCTIVE FEATURES ON BLACK
AND WHITE ROMANIAN COWS FROM ROMANIA NORTH-EAST AREA ......................
V. Maciuc, E. C. Popescu, Liudmila Tarasenko
68
13. RESEARCH REGARDING THE DYNAMIC OF SOME REPRODUCTION INDICES
FOR GREY STEPPE BREED ...............................................................................................................
Ingrid Aspazia Gruber, /. Gflcii, A. Gruber
71
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
14. CONTRIBUTIONS TO THE KNOWLEDGE OF QUANTITATIVE MODIFICATIONS
FROM CHEESES DURING MATURATION PERIOD ..................................................................
M.G. Usturoi
76
15. CHARACTERIZATION OF TEXTURE PROFILE IN HORSE MEAT SLAUGHTERED
IN N-E ROMANIA ...................................................................................................................
80
E.C. Diaconu, Roxana Laziir, Nicoleta Giiinii (Diaconu}, M.M. Ciobanu, P.C. Boi~teanu
16. THE QUALITY OF MEAT TO DIFFERENT TYPE MANGALITSA BREEDS'S ...................
G. Hoha, Monica Pandelea, Elena Costiichescu, C.E. Nistor, E. Miigdici, B. Piisiirin
17. EFFECT OF REARING SYSTEM OF LAYING HENS ON SOME QUALITY PHISICAL
INDICATORS FOR CONSUMPTION EGGS ...................................................................................
Lucia Juliana Nistor (Cotfas}, Andreea Ciitiilina Nistor, M.G. Usturoi
18. MILK IMPORTANCE AS RAW MATERIAL IN PROCESSING OF DAIRY PRODUCTS ...
Roxana Nicoleta Ratu, M.G. Usturoi
84
88
94
19. RESEARCH ON ASSESSMENT OF SENSORY BEEF MEAT SLAUGHTERED IN NE
REGION.....................................................................................................................................................
Nicol eta Giiinii (Diaconu}, Roxana Laziir, E. C. Diaconu, M.M. Ciobanu, P.C. Boi~teanu
98
20. STUDY REGARDING THE FEED INFLUENCE ON GROWING PERFORMANCES AT
BREED CYPRINUS CARPIO, USING FODDER ALLER CLASSIC............................................
M.C. Cioran, E. Miigdici, /.B. Pagu, C.A. Barbacariu, B. Piisiirin
103
21. STUDY REGARDING THE INFLUENCE OF WATER TEMPERATURE ON
HORMONAL STIMULATION OF FEMALES FROM POLYODON SPATHULA BREED
FOR MATURATION OF SEXUAL PRODUCTS .............................................................................
C.-A. Barbacariu, /.B. Pagu, E. Miigdici, M.C. Cioran, B. Piisiirin
106
22. STUDYING THE CHEMICAL COMPOSITION OF NUTRIA MEAT (MYOCASTOR
COYPUS M.) ..............................................................................................................................................
Tatiana Mardari, Doina Leonte
112
23. OBSERVATION ON THE GROWTH PERFORMANCE OF GOLDFISH (Carassius
auratus) REARED IN AQUARIUMS WITH DIFFERENT LEVELS OF VITAMIN C ........
Aurelia Nica, I. Vasilean, Adina Popescu
115
24. EFFECT OF DIFFERENT SOURCES OF LUTEIN IN LAYER DIETS ON EGG QUALITY
V. Bunduc, Rodica Diana Criste, Tatiana Dumitra Panaite, Julia Varzaru, C. Panaite,
M.Pop
25. BIOCHEMICAL PROPERTIES AND VIABILITY OF TWO THERMOPHILIC
LACTIC ACID BACTERIA IN A SINGLE CHAMBER GASTROINTESTINAL TRACT
SIMULATOR-GIS!: PARTIAL CHARACTERIZATIONS ...........................................................
E. Vamanu
26. PRELIMINARY ASPECTS CONCERNING MACROINVERTEBRATES QUALITATIVE
STRUCTURE IN THE DANUBE RIVER..........................................................................................
Adina Popescu, Daniela lbanescu, I. Vasilean, Aurelia Nica
27. COMPARISON OF SOME BODY MEASUREMENTS FOR SAANEN GOATS......................
Murat Yilmaz, H. Erbay Bardakcioglu, Tufan A/tin
119
126
130
134
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
28. STUDIES CONCERNING THE NEPHROTOXIC EFFECTS OF OCHRATOXIN A USING
PIG AS A MODEL ...................................................................................................................................
Daniela E. Marin, Gina C. Pistol, M.A. Gras, Monica Motiu, Ionelia Taranu
138
29. STUDYONTHENUTRITIONALQUALITYOFMARINATEDANDSMOKEDMACKEREL
(SCOMBER SCOMBER) ........................................................................................................................
l.M. Pop, Gabriela Frunzii
143
30. THE IMPACT OF APPLYING THE LIQUID ORGANIC FERTILIZER, OBTAINED
FROM WORMS COMPOST, ON QUALITY OF THE MAIZE.....................................................
Larisa Cremeneac, Tatiana Boclaci
148
31. THE STUDY OF INFLUENCE ORGANIC FERTILIZER OBTAINED FROM WORMS
COMPOST, ON THE QUALITY AND HARVEST OF FODDER BEET ....................................
Tatiana Boclaci, Larisa Cremeneac
154
~-
32. STUDIES REGARDING THE OPPORTUNITY OF ESTABLISHING SOME RURAL
PLATFORMS FOR PROCESSING THE LIVESTOCK WASTE INTO ORGANIC
FERTILIZERS ..........................................................................................................................................
Al.-D. Robu, Carmen-Luiza Costuleanu, I. Gflcii
159
33. THE CHEMICAL COMPOSITION ASSESSMENT OF THE ,FETEASCA NEAGRA.''
AND ,ALIGOTE" GRAPE POMACE FRACTIONS .......................................................................
Silvia Mariana Pascariu, l.M. Pop, Aida Albu
165
34. THE CONTENT OF HEAVY METALS IN THE BEES BODY DEPENDING ON LOCATION
AREA OF HIVES .....................................................................................................................................
Olesea Gliga
169
COUNTY'S TOURISTIC AND
35. THE ASSESSMENT OF TURIST FLOW IN IA~
AGROTOURISTIC BOARDING HOUSES........................................................................................
Cristina Simeanu, D. Simeanu, B. Piisiirin
176
36. IMPROVING OXIDATIVE STABILITY OF BEEF BURGERS UNDER CHILLED STORAGE
USING CEREAL GRAIN FRACTIONS .............................................................................................
Bolbol Ramadan Ramadan, Mohamed Abdel-Hamid Sorour, Mohamed Ali Kelany
182
37. BEEKEEPING STRUCTURE, PROBLEMS AND COLONY LOSSES IN THE AEGEAN
REGION OF TURKEY ...........................................................................................................................
Ucak Koc Aytul, Karacaoglu Mete
188
38. OPTIMISATION OF THE GC METHOD FOR ASSESSING VOLATILE FATTY ACIDS
IN RUMEN LIQUID ...............................................................................................................................
Mariana Ropotii, Ana Ci~mleanu,
Smaranda Toma, Julia Viirzaru
194
39. ASPECTS REGARDING THE FRICTION PROCESS OF THE OPERATING PARTS OF
A DRAWER TYPE PULSATOR OF OWN CONCEPTION..........................................................
C-tin Chirilii, R. Ro~ca
199
40. THE SUCCESS OF EMBRYO TRANSFER IN DAIRY CATTLE RECIPIENT USING BEEF
CATTLE EMBRYOS .............................................................................................................................. .
Tita Damayanti Lestari, Ismudiono lsmudiono, Trilas Sardjito, Pudji Sri an to
203
41. EFFICIENCY OF PRODUCTION FACTOR USAGE IN CORN FARMING AT SERANG
DISTRICT, BANTEN PROVINCE ......................................................................................................
Dian Anggraeni, Tuhpawana P. Sendjaja, Tomy Perdana, Anne Nuraini
209
~
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
42.
THE PARASITISM TYPES AND PERCENTAGE OF PARASITOIDS LIR/OMYZA
TREATMENT PLANTS WITH TOMATO AND CONVENTIONAL INTEGRATED
PEST MANAGEMENT..........................................................................................................................
216
Eva Lienneke Baidenn
43.
RESEARCH REGARDING LIPASE ACTIVITY FROM SOME PLANT SPECIES UNDER
THE INFLUENCE OF SOME EXTERNAL FACTORS..................................................................
223
M.Avramiuc
44.
THE QUALITY AND STABILITY OF WALNUT OIL UNDER THE INFLUENCE OF
STORAGE CONDITIONS .....................................................................................................................
230
Ana Leahu, Mircea Oroian, Sorina Ropciuc
45.
THE EFFECT OF PECTIN USAGE AS FEED ADDITIVE ON PIGS EXCRETION
METABOLISM AND BLOOD BIOCHEMICAL PARAMETERS................................................
Liudmyla 0/exiyivna Tarasenko, Valentina 0/eoivna Rud
237
Scientific Papers-Animal Science Series: Lucrari $tiintifice - Seria Zootehnie, vol. 65
THE SUCCESS OF EMBRYO TRANSFER IN DAIRY
CATTLE RECIPIENT USING BEEF CATTLE EMBRYOS
Tita Damayanti Lestari 1*, Ismudiono lsmudiono\ Trilas Sardjito\
Pudji Srianto 1
1
Department of Veterinary Reproduction, Facuity of Veterinary Medicine,
Universitas Airlangga, Indonesia
Abstract
A field research on "The Success of Embryo Transfer in Dairy Cattle Recipient using Beef
Cattle Embryos" has been conducted. Prior to the research, selection of dairy cow recipients has
been done. It has been selected, from three different farm locations i.e. Bendul Merisi Farm: 9
head; Teaching Farm 5 head and Wonocolo 6 head. The 20 recipients have been estrous
synchronized prior to embryos transferred. Blood samples were collected three times i.e.: at onset of
estrous, at receiving embryo (day 7) and day 21 after estrous, to measure the concentration of
progesterone as the parameters. Further parameters were non return rate and pregnancy rate.
Pregnancy detection was done by rectal exploration on day 90 after transferred of embryos. Result
shows 11 head (55%) dairy cow recipients get pregnant. They are 3 heads from Teaching Farm
(60%),. 4 heads (44.44%) from Bendul Merisi Farm and 4 heads (66.66%) from Wonocolo Farm In
conclusion, dairy cow recipients can accept embryos derived from beef cattle.
Key words: Embryo Transfer, Beef Cattle Embryo, Dairy Cattle Recipient
INTRODUCTION
Increasing meat demand especially from
beef cattle will lead to an open broadly
development of beef cattle business in
Indonesia. Transfer embryo technology will
then become an important role to fasten beef
cattle production to fulfill the demands. Embryo
transfer is a program to implant the embryo
derived from an exotic breed or excellent
production animal to the uterus of recipients.
The success of embryo transfer is depending on
several conditions i.e.: recipients' cow, if they
are not in good condition, the embryo transfer
result will poor; rearing management; culture of
farmer, quite frequent after being transferred,
the cow has to work as draft animal or working
in paddy field.
In Indonesia, mostly beef cattle including
beef cow are proposed to work in the field,
therefore selection of beef cow recipient is
quite difficult [5]. Pregnant beef cow is
frequently use as draft animal as well. The
objective of this research is to fmd out whether
'corresponding author: ismudiono@yahoo.co.id
and titadlestari@gmail.com
The manuscript was received: 12.02.2016
Acceptedfor publication: 01.03.2016
the embryo of beef cattle is transferable to
dairy cow instead of transferring to beef cattle
as the mother.
Existence of fetus within the uterus is
tnight be a problem. Fetus heritages genetically
character from its' father, and it means as a
foreign thing or strange for the mother. If the
mother carmot accept the embryo, she can
reject it. The success of fertilization is followed
by the embryo ability in giving signal to the
maternal system or endometrial mother in term
of blocking corpus luteum (CL) regression. in
order to maintain production of progesterone
by its lutein cells [8].
Existence of CL is important to maintain
pregnancy in all species. Zygote synthesis or
secretes steroid and protein as a sign or signal
of its existence to the maternal system.
These molecules directs' synthesis and
releases luteolytic Prostaglandin F 2 alpha
(PGF2a) in uterus to avoid CL regression [2].
During critical period in uterus, zygote
has to covered endometrial maternal system
in producing PGF 2 alpha. In sow, the
process of producing PGF 2 alpha is done by
multiple zygotes, whereas in mare, this
process is conducted by zygotes migration.
Zygote critical period is to block luteolysis
-203-
University of Agricultural Sciences and Veterinary Medicine lasi
and allow a pregnancy to be proceed. This is
called maternal recognition of pregnancy.
Maternal recognition of pregnancy in cow
is between days 16 -19 of pregnancy [10].
Bovine zygote produces an amount of acid
protein with low molecule weight such as:
bovine trophoblast protein-! (bTP-1). This
protein is classified within interferon-tau and
named bovine interferon-tau (blFN-'t).
Endomerium
pregnant
cow
produces
endometrium prostaglandin synthesis-inhibitor
(EPSI) wich is specifically depress PGF 2 alpha
synthesis [2]. This inhibitor arises as linoleat
acid. Ratio linoleat acid and arachidonate acid
(PGF2 alpha precursor) is higher in pregnant
cow compare to non pregnant cow. This is lead
to the importance to chance lipid composition in
endometrium tissue in maternal recognition of
pregnancy of cow [9].
Maternal recognition of pregnancy is
meant as an interaction between maternal
unit and the zygote products of protein. This
interaction gives a signal to the mother to
maintain the pregnancy
[10].
This
communication happens in several critical
stages during pregnancy, but one important
stage of this process in ruminant involved
paracrine regulation. Zygote in endometium
regulates paracrine to weakened secretion of
PGF 2 alpha which has luteolytic character.
According to Thatcher (1986) luteolytic
effect which is belong to the zygote leads to
keep the existence ofCL in sheep and cow.
METHOD
This research used twenty head of FH
dairy cow. All those cows were implanted by
embryos through embryo transfer technique.
Frozen beef embryos derived from Simental
breed were purchased from BET Cipelang
West Java.
All cows were measured the progesterone
concentration to defme their reproduction
activity. Individual observation was also
done by rectal palpation to learn its corpus
luteum. Prior to transfer of embryo, all cows
were estrous synchronized using PGF2a intra
vulva. PGF2a was given twice i.e.: 11 days
before estrous and three days post
administration. And it is interpreted by day 0
of the estrous cycle. All cows were then
transferred by embryos on day 6-7 (depends
of the embryos age).
Blood samples from the cow were done to
measure their progesterone concentration and
were collected three times i.e.: at onset of
estrous, at transferring time and 21 days after
estrous to observe their progesterone
concentration. On day 6 - 7, after onset of
estrous, meant day 0, the beef cattle embryos
were transferred to dairy cow recipient. At the
same time of transferring, cows were injected
by hCG (human chorionic gonadothrophin).
Estrous has detected on 21 - 24 days after
being transferred due to non return rate.
Detection of pregnancy was done by
rectal exploration on day 90 to define
pregnancy. Parameter of observation were
progesterone concentration on day 0, day 7
(transferring time) and day 21 after transfer.
Data collected was analyzed descriptively.
Pregnancy rate and calves born sex were
analyzed by Chi-square.
RESULTS AND DISCUSSIONS
Research was conducted in three dairy
farms with the same character. Selected was
done to fmd dairy cows as recipients.
Selection was based on the conditions of
recipient's procurements, such as: physically
health, history of diseases especially
reproduction disorder. Selection was directed
to examine their reproductive organs and
learn their normal estrous cycle. Result of
selection is shown in Table 1.
Table 1 shows selection results of dairy cows
recipients
No
Name of Farm
1.
2.
3.
Teachinq Farm- Gresik
Bendul Merisi Dairv Farm
Wonocolo Dairy Farm
Total
Head of
Recipients
5
9
6
20
Prior to transferred, all the recipients
were synchronized the estrus using PGF2a.
Embryos transferred to the recipient were
conducted on day 7 after estrus (in
accordance with the age of the embryo).
Pregnancy examinations conducted by rectal
exploration, at day 90 post-transfer. The
results obtained are 11 individuals (55%)
recipients become pregnant with details three
-204-
Scientific Papers-Animal Science Series: Lucrari §>tiintifice - Seria Zootehnie, vol. 65
head from Teaching Farm- Gresik or 60%
(3/5); four head or 44.44% (4/9) from Bendul
Merisi Dairy Farm and four head or 66.66%
(4/6) from Wonocolo Dairy Farm acceptor
become pregnant. These results are presented
in Table 2 and the percentage graph is can be
seen in Figure I.
Table 2 Pregnancy rate recognized by rectal palpation on day 90 post transferred in dairy cow recipients
No.
1.
2.
3.
Farm
Taman Ternak Pendidikan- Gresik
Peternakan Sapi Perah Bendul Merisi
Peternakan Sapi Perah Wonocolo
Total
Total pregnant recipients (head)
3(60%)
4 (44.44°/
PRINT ISSN 1454-7368
ELECTRONIC ISSN 2067-2330
CD-ROM ISSN 2284-6964
UNIVERSITATEA DE $TIINJE ACRICOLE
$1 MEDICINA VETERINARA
ION IONESCU DE ~A
BRAD" DIN IA$1
University of Applied Life Sciences and Environment
11
SCIENTIFIC PAPERS
ANIMAL SCIENCES
LUCRAiu fTIINfiFICE
SERIA ZOOTEHNIE
VOL. 65 (21)
EDITURA " ION IONESCU DE LA BRAD"
,
2016
IA~
COORDINATORS:
Editor-in-chief: Prof.univ.dr. Vasile VINTU, U.S.A.M.V. lasi, ROMANIA
Associate Editor: Prof.univ.dr. Constantin LEONTE, U.S.A.M.V. lasi, ROMANIA
MEMBERS:
-
Prof.univ.dr.
Prof.univ.dr.
Prof.univ.dr.
Prof.univ.dr.
Teodor ROBU- U.S.A.M.V. lasi, ROMANIA
Lucia DRAGHIA- U.S.A.M.V. lasi, ROMANIA
Benone PASARIN- U.S.A.M.V. lasi, ROMANIA
Liviu MIRON- U.S.A.M.V. lasi, ROMANIA
EDITORIAL BOARD OF THE ANIMAL SCIENCE SERIES:
Editor-in-chief: Prof.univ.dr. Benone PASARIN, U.S.A.M.V. lasi, ROMANIA
Associate Editor: Prof.univ.dr. Constantin PASCAL, U.S.A.M.V. Iasi, ROMANIA
EDITORS:
-
Prof.univ.dr. dr. H.C. Liviu AI. MARGHIT~U.S.A.M.V. Cluj-Napoca, ROMANIA
U.S.A.M.V. lai, ROMANIA
Prof.univ.dr. loan VACRU-OPI~
Prof.univ.dr. loan GILCA- U.S.A.M.V. lasi, ROMANIA
Asist.univ.dr. Razvan Mihail RADU-RUSU- U.S.A.M.V. lasi, ROMANIA
Seflucr.dr. Bogdan Vlad AVARVAREI- U.S.A.M.V.Iasi, ROMANIA
Dr. H.C. Yves NYS- L'institut National de Ia Rechearche Agronomique (INRA), FRANCE
Prof.univ.dr. Mohamed A. KENAWI- Minia University, EGYPT
Prof.univ.dr. Otto SZENCI- Szent Istvan University. Budapest- HUNGARY
Prof.univ.dr. Mark RUSSELL- College of Agriculture, Purdue University, Indiana, USA
Prof.univ.dr. Frans;oise PICARD- Universite d'Angers, FRANCE
REVIEWERS:
-
Prof.univ.dr. Victor CRISTEA- Univ. "Dunarea de Jos" GALATI, ROMANIA
Prof.univ.dr. llie VAN- U.S.A.M.V. BUCURESTI, ROMANIA
Prof.univ.dr. Augustin VLAIC- U.S.A.M.V. CLUJ NAPOCA, ROMANIA
Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Prof.univ.dr. Paul Corneliu BOI~TEANUProf.univ.dr. loan Mircea POP- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Prof.univ.dr. Marius Giorgi USTUROI- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
REPUBLIC OF MOLProf.univ.dr. Nicolae EREMIA - Universitatea Agrara de Stat Chi~nau,
DOVA
Prof.univ.dr. Nicolae PACALA- U.S.A.M.V.B. TIMISOARA, ROMANIA
Prof.univ.dr. ~teofil
CREANGA- Facultatea de Zootehnie, U.S.A.M.V. lAS!, ROMANIA
Lector- Dr. Hendronoto A.W. LENGKEY- Padjadjaran University, INDONESIA
©2007 UASVM
la~i
I Last update: May, 04, 2016
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
CUPRINS (TABLE OF CONTENTS)
1.
ATTEMPET TO PRODUCE COLD RESISTANCE RABBITS ....................................................
3
T.M. El-Sheikh, T.S. T. Seleem
2.
EVALUATION OF THE REPRODUCTION FUNCTION OF GOATS IN ROMANIA ...........
9
C. Pascal, N. Zaharia
3.
INVESTIGATION OF ZEARALENONE TOLERANCE LIMIT IN THE FEEDSTUFFS FOR
WEANED PIGS........................................................................................................................................
14
lonelia Taranu, M.A. Gras, Gina Cecilia Pistol, Monica Motiu, Daniela Marin,
Mariana Stancu
4.
EFFECT OF GENOTYPE AND NATURAL OR ARTIFICIAL INSEMINATION ON
INDIGENOUS AND ADDAPTED RABBIT PERFORMANCE ..................................................
19
T.M. El-Sheikh, T.S. T. Seleem
5.
EFFECT OF USING RAW MATERIALS RICH IN XANTHOPHYLL$ IN LAYER DIETS
ON EGG QUALITY..................................................................................................................................
25
T.D. Panaite, R.D. Criste, /. Varzaru, M. Cornescu, M. Olteanu
6.
RELATIONSHIP BETWEEN LIPTEN HORMONE CONCENTRATION AND RABBIT
REPRODUCTIVITY ...............................................................................................................................
32
I.A. El-Wardany, N.F.A. Saleh, T.S. T. Seleem, A.M.H. Ahmed
7.
EFFECT OF SPERM CONCENTRATION AND SITE OF INSEMINATION ON
CONCEPTION RATE OF RABBITS ..................................................................................................
38
Rowida M. Rriad, T.S. T. Seleem, KH. El-Kholy, Z.M. Rawash
8.
STUDY ON THE INFLUENCE OF THE INCUBATION EGGS KEEPING POSITION
DURING STORAGE AND THEIR OLDNESS ON HATCHING RESULTS OF THE
BALOTESTI QUAILS POPULATION ...............................................................................................
G. Nica, L. /onitii, Elena Popescu-Mil~an,
9.
44
N. Stanciu
CORELATION BETWEEN THE CONTENT OF HEAVY METALS (Pb, Cd and Cu) IN
THE BEE PRODUCTS AND THE BEES BODY.............................................................................
49
Olesea Gliga, Valentina Cebotari, I. Buzu
10. THE MICROELEMENT MOLYBDENUM (Mo) IN NUTRITIONAL SUPPLEMENT
FOR BEES.................................................................................................................................................
Valentina Cebotari, /. Todera~,
56
A. Gulea, /. Buzu
11. MORPHOLOGICAL STUDY CONCERNING THE REPRODUCTION HORSES FROM
ROMANIAN TRAPA~ BREED FROM JEGALJA STUD ..............................................................
M.G. Doli~,
C.E. Nistor, V. Biicilii, C. $onea
64
12. STUDY OF SOME PRODUCTIVE AND REPRODUCTIVE FEATURES ON BLACK
AND WHITE ROMANIAN COWS FROM ROMANIA NORTH-EAST AREA ......................
V. Maciuc, E. C. Popescu, Liudmila Tarasenko
68
13. RESEARCH REGARDING THE DYNAMIC OF SOME REPRODUCTION INDICES
FOR GREY STEPPE BREED ...............................................................................................................
Ingrid Aspazia Gruber, /. Gflcii, A. Gruber
71
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
14. CONTRIBUTIONS TO THE KNOWLEDGE OF QUANTITATIVE MODIFICATIONS
FROM CHEESES DURING MATURATION PERIOD ..................................................................
M.G. Usturoi
76
15. CHARACTERIZATION OF TEXTURE PROFILE IN HORSE MEAT SLAUGHTERED
IN N-E ROMANIA ...................................................................................................................
80
E.C. Diaconu, Roxana Laziir, Nicoleta Giiinii (Diaconu}, M.M. Ciobanu, P.C. Boi~teanu
16. THE QUALITY OF MEAT TO DIFFERENT TYPE MANGALITSA BREEDS'S ...................
G. Hoha, Monica Pandelea, Elena Costiichescu, C.E. Nistor, E. Miigdici, B. Piisiirin
17. EFFECT OF REARING SYSTEM OF LAYING HENS ON SOME QUALITY PHISICAL
INDICATORS FOR CONSUMPTION EGGS ...................................................................................
Lucia Juliana Nistor (Cotfas}, Andreea Ciitiilina Nistor, M.G. Usturoi
18. MILK IMPORTANCE AS RAW MATERIAL IN PROCESSING OF DAIRY PRODUCTS ...
Roxana Nicoleta Ratu, M.G. Usturoi
84
88
94
19. RESEARCH ON ASSESSMENT OF SENSORY BEEF MEAT SLAUGHTERED IN NE
REGION.....................................................................................................................................................
Nicol eta Giiinii (Diaconu}, Roxana Laziir, E. C. Diaconu, M.M. Ciobanu, P.C. Boi~teanu
98
20. STUDY REGARDING THE FEED INFLUENCE ON GROWING PERFORMANCES AT
BREED CYPRINUS CARPIO, USING FODDER ALLER CLASSIC............................................
M.C. Cioran, E. Miigdici, /.B. Pagu, C.A. Barbacariu, B. Piisiirin
103
21. STUDY REGARDING THE INFLUENCE OF WATER TEMPERATURE ON
HORMONAL STIMULATION OF FEMALES FROM POLYODON SPATHULA BREED
FOR MATURATION OF SEXUAL PRODUCTS .............................................................................
C.-A. Barbacariu, /.B. Pagu, E. Miigdici, M.C. Cioran, B. Piisiirin
106
22. STUDYING THE CHEMICAL COMPOSITION OF NUTRIA MEAT (MYOCASTOR
COYPUS M.) ..............................................................................................................................................
Tatiana Mardari, Doina Leonte
112
23. OBSERVATION ON THE GROWTH PERFORMANCE OF GOLDFISH (Carassius
auratus) REARED IN AQUARIUMS WITH DIFFERENT LEVELS OF VITAMIN C ........
Aurelia Nica, I. Vasilean, Adina Popescu
115
24. EFFECT OF DIFFERENT SOURCES OF LUTEIN IN LAYER DIETS ON EGG QUALITY
V. Bunduc, Rodica Diana Criste, Tatiana Dumitra Panaite, Julia Varzaru, C. Panaite,
M.Pop
25. BIOCHEMICAL PROPERTIES AND VIABILITY OF TWO THERMOPHILIC
LACTIC ACID BACTERIA IN A SINGLE CHAMBER GASTROINTESTINAL TRACT
SIMULATOR-GIS!: PARTIAL CHARACTERIZATIONS ...........................................................
E. Vamanu
26. PRELIMINARY ASPECTS CONCERNING MACROINVERTEBRATES QUALITATIVE
STRUCTURE IN THE DANUBE RIVER..........................................................................................
Adina Popescu, Daniela lbanescu, I. Vasilean, Aurelia Nica
27. COMPARISON OF SOME BODY MEASUREMENTS FOR SAANEN GOATS......................
Murat Yilmaz, H. Erbay Bardakcioglu, Tufan A/tin
119
126
130
134
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
28. STUDIES CONCERNING THE NEPHROTOXIC EFFECTS OF OCHRATOXIN A USING
PIG AS A MODEL ...................................................................................................................................
Daniela E. Marin, Gina C. Pistol, M.A. Gras, Monica Motiu, Ionelia Taranu
138
29. STUDYONTHENUTRITIONALQUALITYOFMARINATEDANDSMOKEDMACKEREL
(SCOMBER SCOMBER) ........................................................................................................................
l.M. Pop, Gabriela Frunzii
143
30. THE IMPACT OF APPLYING THE LIQUID ORGANIC FERTILIZER, OBTAINED
FROM WORMS COMPOST, ON QUALITY OF THE MAIZE.....................................................
Larisa Cremeneac, Tatiana Boclaci
148
31. THE STUDY OF INFLUENCE ORGANIC FERTILIZER OBTAINED FROM WORMS
COMPOST, ON THE QUALITY AND HARVEST OF FODDER BEET ....................................
Tatiana Boclaci, Larisa Cremeneac
154
~-
32. STUDIES REGARDING THE OPPORTUNITY OF ESTABLISHING SOME RURAL
PLATFORMS FOR PROCESSING THE LIVESTOCK WASTE INTO ORGANIC
FERTILIZERS ..........................................................................................................................................
Al.-D. Robu, Carmen-Luiza Costuleanu, I. Gflcii
159
33. THE CHEMICAL COMPOSITION ASSESSMENT OF THE ,FETEASCA NEAGRA.''
AND ,ALIGOTE" GRAPE POMACE FRACTIONS .......................................................................
Silvia Mariana Pascariu, l.M. Pop, Aida Albu
165
34. THE CONTENT OF HEAVY METALS IN THE BEES BODY DEPENDING ON LOCATION
AREA OF HIVES .....................................................................................................................................
Olesea Gliga
169
COUNTY'S TOURISTIC AND
35. THE ASSESSMENT OF TURIST FLOW IN IA~
AGROTOURISTIC BOARDING HOUSES........................................................................................
Cristina Simeanu, D. Simeanu, B. Piisiirin
176
36. IMPROVING OXIDATIVE STABILITY OF BEEF BURGERS UNDER CHILLED STORAGE
USING CEREAL GRAIN FRACTIONS .............................................................................................
Bolbol Ramadan Ramadan, Mohamed Abdel-Hamid Sorour, Mohamed Ali Kelany
182
37. BEEKEEPING STRUCTURE, PROBLEMS AND COLONY LOSSES IN THE AEGEAN
REGION OF TURKEY ...........................................................................................................................
Ucak Koc Aytul, Karacaoglu Mete
188
38. OPTIMISATION OF THE GC METHOD FOR ASSESSING VOLATILE FATTY ACIDS
IN RUMEN LIQUID ...............................................................................................................................
Mariana Ropotii, Ana Ci~mleanu,
Smaranda Toma, Julia Viirzaru
194
39. ASPECTS REGARDING THE FRICTION PROCESS OF THE OPERATING PARTS OF
A DRAWER TYPE PULSATOR OF OWN CONCEPTION..........................................................
C-tin Chirilii, R. Ro~ca
199
40. THE SUCCESS OF EMBRYO TRANSFER IN DAIRY CATTLE RECIPIENT USING BEEF
CATTLE EMBRYOS .............................................................................................................................. .
Tita Damayanti Lestari, Ismudiono lsmudiono, Trilas Sardjito, Pudji Sri an to
203
41. EFFICIENCY OF PRODUCTION FACTOR USAGE IN CORN FARMING AT SERANG
DISTRICT, BANTEN PROVINCE ......................................................................................................
Dian Anggraeni, Tuhpawana P. Sendjaja, Tomy Perdana, Anne Nuraini
209
~
Lucrari Stiintifice-Seria Zootehnie, Vol. 65
42.
THE PARASITISM TYPES AND PERCENTAGE OF PARASITOIDS LIR/OMYZA
TREATMENT PLANTS WITH TOMATO AND CONVENTIONAL INTEGRATED
PEST MANAGEMENT..........................................................................................................................
216
Eva Lienneke Baidenn
43.
RESEARCH REGARDING LIPASE ACTIVITY FROM SOME PLANT SPECIES UNDER
THE INFLUENCE OF SOME EXTERNAL FACTORS..................................................................
223
M.Avramiuc
44.
THE QUALITY AND STABILITY OF WALNUT OIL UNDER THE INFLUENCE OF
STORAGE CONDITIONS .....................................................................................................................
230
Ana Leahu, Mircea Oroian, Sorina Ropciuc
45.
THE EFFECT OF PECTIN USAGE AS FEED ADDITIVE ON PIGS EXCRETION
METABOLISM AND BLOOD BIOCHEMICAL PARAMETERS................................................
Liudmyla 0/exiyivna Tarasenko, Valentina 0/eoivna Rud
237
Scientific Papers-Animal Science Series: Lucrari $tiintifice - Seria Zootehnie, vol. 65
THE SUCCESS OF EMBRYO TRANSFER IN DAIRY
CATTLE RECIPIENT USING BEEF CATTLE EMBRYOS
Tita Damayanti Lestari 1*, Ismudiono lsmudiono\ Trilas Sardjito\
Pudji Srianto 1
1
Department of Veterinary Reproduction, Facuity of Veterinary Medicine,
Universitas Airlangga, Indonesia
Abstract
A field research on "The Success of Embryo Transfer in Dairy Cattle Recipient using Beef
Cattle Embryos" has been conducted. Prior to the research, selection of dairy cow recipients has
been done. It has been selected, from three different farm locations i.e. Bendul Merisi Farm: 9
head; Teaching Farm 5 head and Wonocolo 6 head. The 20 recipients have been estrous
synchronized prior to embryos transferred. Blood samples were collected three times i.e.: at onset of
estrous, at receiving embryo (day 7) and day 21 after estrous, to measure the concentration of
progesterone as the parameters. Further parameters were non return rate and pregnancy rate.
Pregnancy detection was done by rectal exploration on day 90 after transferred of embryos. Result
shows 11 head (55%) dairy cow recipients get pregnant. They are 3 heads from Teaching Farm
(60%),. 4 heads (44.44%) from Bendul Merisi Farm and 4 heads (66.66%) from Wonocolo Farm In
conclusion, dairy cow recipients can accept embryos derived from beef cattle.
Key words: Embryo Transfer, Beef Cattle Embryo, Dairy Cattle Recipient
INTRODUCTION
Increasing meat demand especially from
beef cattle will lead to an open broadly
development of beef cattle business in
Indonesia. Transfer embryo technology will
then become an important role to fasten beef
cattle production to fulfill the demands. Embryo
transfer is a program to implant the embryo
derived from an exotic breed or excellent
production animal to the uterus of recipients.
The success of embryo transfer is depending on
several conditions i.e.: recipients' cow, if they
are not in good condition, the embryo transfer
result will poor; rearing management; culture of
farmer, quite frequent after being transferred,
the cow has to work as draft animal or working
in paddy field.
In Indonesia, mostly beef cattle including
beef cow are proposed to work in the field,
therefore selection of beef cow recipient is
quite difficult [5]. Pregnant beef cow is
frequently use as draft animal as well. The
objective of this research is to fmd out whether
'corresponding author: ismudiono@yahoo.co.id
and titadlestari@gmail.com
The manuscript was received: 12.02.2016
Acceptedfor publication: 01.03.2016
the embryo of beef cattle is transferable to
dairy cow instead of transferring to beef cattle
as the mother.
Existence of fetus within the uterus is
tnight be a problem. Fetus heritages genetically
character from its' father, and it means as a
foreign thing or strange for the mother. If the
mother carmot accept the embryo, she can
reject it. The success of fertilization is followed
by the embryo ability in giving signal to the
maternal system or endometrial mother in term
of blocking corpus luteum (CL) regression. in
order to maintain production of progesterone
by its lutein cells [8].
Existence of CL is important to maintain
pregnancy in all species. Zygote synthesis or
secretes steroid and protein as a sign or signal
of its existence to the maternal system.
These molecules directs' synthesis and
releases luteolytic Prostaglandin F 2 alpha
(PGF2a) in uterus to avoid CL regression [2].
During critical period in uterus, zygote
has to covered endometrial maternal system
in producing PGF 2 alpha. In sow, the
process of producing PGF 2 alpha is done by
multiple zygotes, whereas in mare, this
process is conducted by zygotes migration.
Zygote critical period is to block luteolysis
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University of Agricultural Sciences and Veterinary Medicine lasi
and allow a pregnancy to be proceed. This is
called maternal recognition of pregnancy.
Maternal recognition of pregnancy in cow
is between days 16 -19 of pregnancy [10].
Bovine zygote produces an amount of acid
protein with low molecule weight such as:
bovine trophoblast protein-! (bTP-1). This
protein is classified within interferon-tau and
named bovine interferon-tau (blFN-'t).
Endomerium
pregnant
cow
produces
endometrium prostaglandin synthesis-inhibitor
(EPSI) wich is specifically depress PGF 2 alpha
synthesis [2]. This inhibitor arises as linoleat
acid. Ratio linoleat acid and arachidonate acid
(PGF2 alpha precursor) is higher in pregnant
cow compare to non pregnant cow. This is lead
to the importance to chance lipid composition in
endometrium tissue in maternal recognition of
pregnancy of cow [9].
Maternal recognition of pregnancy is
meant as an interaction between maternal
unit and the zygote products of protein. This
interaction gives a signal to the mother to
maintain the pregnancy
[10].
This
communication happens in several critical
stages during pregnancy, but one important
stage of this process in ruminant involved
paracrine regulation. Zygote in endometium
regulates paracrine to weakened secretion of
PGF 2 alpha which has luteolytic character.
According to Thatcher (1986) luteolytic
effect which is belong to the zygote leads to
keep the existence ofCL in sheep and cow.
METHOD
This research used twenty head of FH
dairy cow. All those cows were implanted by
embryos through embryo transfer technique.
Frozen beef embryos derived from Simental
breed were purchased from BET Cipelang
West Java.
All cows were measured the progesterone
concentration to defme their reproduction
activity. Individual observation was also
done by rectal palpation to learn its corpus
luteum. Prior to transfer of embryo, all cows
were estrous synchronized using PGF2a intra
vulva. PGF2a was given twice i.e.: 11 days
before estrous and three days post
administration. And it is interpreted by day 0
of the estrous cycle. All cows were then
transferred by embryos on day 6-7 (depends
of the embryos age).
Blood samples from the cow were done to
measure their progesterone concentration and
were collected three times i.e.: at onset of
estrous, at transferring time and 21 days after
estrous to observe their progesterone
concentration. On day 6 - 7, after onset of
estrous, meant day 0, the beef cattle embryos
were transferred to dairy cow recipient. At the
same time of transferring, cows were injected
by hCG (human chorionic gonadothrophin).
Estrous has detected on 21 - 24 days after
being transferred due to non return rate.
Detection of pregnancy was done by
rectal exploration on day 90 to define
pregnancy. Parameter of observation were
progesterone concentration on day 0, day 7
(transferring time) and day 21 after transfer.
Data collected was analyzed descriptively.
Pregnancy rate and calves born sex were
analyzed by Chi-square.
RESULTS AND DISCUSSIONS
Research was conducted in three dairy
farms with the same character. Selected was
done to fmd dairy cows as recipients.
Selection was based on the conditions of
recipient's procurements, such as: physically
health, history of diseases especially
reproduction disorder. Selection was directed
to examine their reproductive organs and
learn their normal estrous cycle. Result of
selection is shown in Table 1.
Table 1 shows selection results of dairy cows
recipients
No
Name of Farm
1.
2.
3.
Teachinq Farm- Gresik
Bendul Merisi Dairv Farm
Wonocolo Dairy Farm
Total
Head of
Recipients
5
9
6
20
Prior to transferred, all the recipients
were synchronized the estrus using PGF2a.
Embryos transferred to the recipient were
conducted on day 7 after estrus (in
accordance with the age of the embryo).
Pregnancy examinations conducted by rectal
exploration, at day 90 post-transfer. The
results obtained are 11 individuals (55%)
recipients become pregnant with details three
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Scientific Papers-Animal Science Series: Lucrari §>tiintifice - Seria Zootehnie, vol. 65
head from Teaching Farm- Gresik or 60%
(3/5); four head or 44.44% (4/9) from Bendul
Merisi Dairy Farm and four head or 66.66%
(4/6) from Wonocolo Dairy Farm acceptor
become pregnant. These results are presented
in Table 2 and the percentage graph is can be
seen in Figure I.
Table 2 Pregnancy rate recognized by rectal palpation on day 90 post transferred in dairy cow recipients
No.
1.
2.
3.
Farm
Taman Ternak Pendidikan- Gresik
Peternakan Sapi Perah Bendul Merisi
Peternakan Sapi Perah Wonocolo
Total
Total pregnant recipients (head)
3(60%)
4 (44.44°/