Buffer Elektroforesis • 14,4 gram glycine •

LAMPIRAN 7

  Lampiran 1. Kurva Standar Protein

• Pembuatan Reagen Bradford

  Sebanyak 100 mg Coomassie Briliant Blue G-250 dilarutkan dalam 50 ml ethanol 95%.

  Larutan tersebut kemudian ditambah dengan 100 ml asam fosfor 85%. Setelah

  tercampur rata, larutan diencerkan dengan menggunakan ddH 2 O hingga 1000 ml.

  

Reagen harus disaring terlebih dahulu dengan menggunakan kertas saring sebelum

digunakan (Bollag & Stuart, 1991).

• Pembuatan Kurva Standar Protein

  BSA (Bovine Serum Albumin) ditimbang sebanyak 100 mg dan dilarutkan dalam 100 ml

  ddH O hingga menjadi larutan stok BSA 1 mg/ml. Untuk membuat kurva standar

  2

protein, dibutuhkan larutan protein dari konsentrasi 0 mg/ml; 0,1 mg/ml; 0,2 mg/ml; 0,3

mg/ml; 0,4 mg/ml; 0,5 mg/ml; 0,6 mg/ml; 0,7 mg/ml; 0,8 mg/ml; 0,9 mg/ml; dan 1

mg/ml. Dari masing-masing konsentrasi diambil sebanyak 0,1 ml dan direaksikan

dengan 5 ml reagen Bradford selama 5 menit. Larutan kemudian dispektrofotometri

pada panjang gelombang 595 nm (Bradford, 1976).

  Lampiran 2. Komposisi Reagen Analisis Molekuler NAMA LARUTAN KOMPOSISI Larutan A (Acrylamide Stock Solution), 100 ml 30% acrylamide, 0,8%

• bis- acrylamide

  29,2 gram

• acrylamide

  0,8 gram bis-acrylamide Larutan B

• (4x Separating Gel Buffer), 100 ml • 75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M • 4 ml 10% SDS --- 0,4 % 21 ml ddH

  2 O Larutan C

• (4x Stacking Gel Buffer), 100 ml • 50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M • 4 ml 10% SDS --- 0,4 % 46 ml ddH

  2 O 10% Ammonium Persulphate

• (APS) • 0,5 gram APS 5 ml DDH

  2 O

• Buffer Elektroforesis • 3 gr Tris --- 25Mm 14,4 gram glycine
• 192Mm
• 1 gram SDS --- 0,1%

  ddH

  2 O sampai 1 liter

• Sampel Buffer • 3,028 gram Tris • 0,25 ml larutan C • 0,4 ml 10% SDS
• 25 ml gliserol

  0,2 mg

• bromophenol blue

  0,031 gram Dithiothreitol (DTT)

  Lampiran 3. Kurva Standar BSA (Bovine Serume Albumin) Low Molecular Weight

  Lampiran 4. Analisis Data Hasil Penelitian Adonan tepung terigu yang mengandung Kayu Manis hardness a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 adonan kontrol 2 30.1305 adonan kayu manis 1,5%

2 48.2095

adonan kayu manis 3% 2 108.8200 Sig. 1.000 1.000 1.000

  Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 2.000. Lampiran 5. Analisis Data Hasil Penelitian Roti Kayu Manis

• Analisis Tekstur

  hardness roti kontrol a,b

  Duncan Subset perlakuan N

  1

  2 proofing 6 24.1257 mixing 6 24.4098 baking 6 236.0733

  Sig. .979 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 341.551.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b. Alpha = .05.

  hardness roti 1,5% kayu manis a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 27.7132 proving 6 28.4843 baking 6 216.5800 Sig.

  .910 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 134.965.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b.

  Alpha = .05.

  hardness roti kayu manis 3% a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 31.7583 proving 6 42.7450 baking 6 163.0567 Sig.

  .079 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 102.098.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b.

  hardness mixing a,b

  Duncan Subset konsentrasi N

  1

  2 roti kontrol 6 24.4098 roti kayu manis 1,5% 6 27.7132 roti kayu manis 3% 6 31.7583 Sig.

  .101 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 10.734.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b.

  Alpha = .05.

  hardness proving a,b

  Duncan Subset konsentrasi N

  1

  2

  3 roti kontrol 6 24.1257 roti kayu manis 1,5% 6 28.4843 roti kayu manis 3% 6 42.7450

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 5.622.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  hardness baking a,b

  Duncan Subset konsentrasi N

  1

  2 roti kayu manis 3% 6 163.0567 roti kayu manis 1,5% 6 216.5800 roti kontrol 6 236.0733

  Sig. 1.000 .175 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 562.258.

  a. Uses Harmonic Mean Sample Size = 6.000.

  b. Alpha = .05.

• Analisis Warna

L

  a Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 roti kayu manis 3% 6 64.6950 roti kayu manis 1,5% 6 67.8733 roti kontrol

6 76.5400

Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000. a a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 roti kontrol 6 -3.4533 roti kayu manis 1,5% 6 3.2183 roti kayu manis 3%

6 6.6417

Sig. 1.000 1.000 1.000

  Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000. b a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2 roti kayu manis 1,5% 6 31.8833 roti kayu manis 3% 6 32.2250 roti kontrol 6 37.8267 Sig.

  

.697 1.000

Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

• Analisis Volume Pengembangan

  vol_pengembangan roti kontrol a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 3 16.5720 proving 3 21.4963 baking 3 34.8487

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 1.284.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  b.

  Alpha = .05.

  vol_pengembangan roti kayu manis 1,5% a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 3 15.0570 proving 3 19.7917 baking 3 29.8293

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = .426.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  b.

  Alpha = .05.

  vol_pengembangan roti kayu manis 3% a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 3 13.8257 proving 3 17.7560 baking 3 25.9943

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = .393.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  b.

  Alpha = .05.

  vol_pengembangan mixing a,b

  Duncan Subset konsentrasi N

  1

  2 roti kayu manis 3% 3 13.8257 roti kayu manis 1,5% 3 15.0570 15.0570 roti kontrol 3 16.5720 Sig.

  .094 .050 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = .576.

  a. Uses Harmonic Mean Sample Size = 3.000.

  b.

  Alpha = .05.

  vol_pengembangan proving a,b

  Duncan Subset konsentrasi N

  1

  2

  3 roti kayu manis 3% 3 17.7560 roti kayu manis 1,5% 3 19.7917 roti kontrol 3 21.4963

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = .173.

  a. Uses Harmonic Mean Sample Size = 3.000.

  b.

  Alpha = .05.

  vol_pengembangan baking a,b

  Duncan Subset konsentrasi N

  1

  2

  3 roti kayu manis 3% 3 25.9943 roti kayu manis 1,5% 3 29.8293 roti kontrol 3 34.8487

  Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 1.354.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

• Analisis Aktivitas Antioksidan

  antioksidan roti kontrol a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 4.0183 proving 6 5.2250 5.2250 baking 6 6.4917

  Sig. .257 .235 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 3.150.

  a. Uses Harmonic Mean Sample Size = 6.000.

  b. Alpha = .05.

  antioksidan roti kayu manis 1,5% a,b

  Duncan Subset perlakuan N

  1 mixing 6 8.2717 proving 6 10.4100 baking 6 12.7683 Sig.

  .096 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 17.393.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b.

  Alpha = .05.

  antioksidan roti kayu manis 3% a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 12.7033 proving 6 18.3750 18.3750 baking 6 23.4183 Sig.

  .143 .189 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 40.374.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b.

  Alpha = .05.

  antioksidan mixing a,b

  Duncan Subset konsentrasi N

  1

  2 roti kontrol 6 4.0183 roti kayu manis 1,5% 6 8.2717 8.2717 roti kayu manis 3% 6 12.7033

  Sig. .088 .077 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 16.348.

  a. Uses Harmonic Mean Sample Size = 6.000.

  b.

  Alpha = .05.

  antioksidan proving a,b

  Duncan Subset konsentrasi N

  1

  2

  3 roti kontrol 6 5.2250 roti kayu manis 1,5% 6 10.4100 roti kayu manis 3% 6 18.3750 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 15.883.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  b. Alpha = .05.

  antioksidan baking a,b

  Duncan Subset konsentrasi N

  1

  2 roti kontrol 6 6.4917 roti kayu manis 1,5% 6 12.7683 roti kayu manis 3% 6 23.4183 Sig.

  .061 1.000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 28.686.

  a. Uses Harmonic Mean Sample Size = 6.000.

  b. Alpha = .05.