INTAKE OF SARDINELLA LONGICEPS OIL AS ANTI DISLIPIDEMIA THROUGH DECREASE OF MDA ON RAT WISTAR.
INTAKE OF SARDINELLA LONGICEPS OIL AS ANTI DISLIPIDEMIA
THROUGH DECREASE OF MDA ON RAT WISTAR
Sri Wahjuni
Biochemistry Laboratory Chemistry Departement Faculty of Math and Science University
INTRODUCTION
Nowdays, fish consumption behaviour
needs to be improve since the changes of
consumption patern to instan food. Fish,
especially from deep sea is good for health,
due to the fish rich of polyunsaturated fatty
acid (omega-3). The author want to
investigate the effect of anti dislipidemia of
Sardinella longiceps Oil (SLO) through
decrease of MDA levels.
RESEARCH METHODS
This is a true experimental study with
randomized pre and postest control group
design. This research employing 50 Wistar
rats divided into 5 groups, i.e. control group
(0% SLO), treatment group 1 (10% SLO),
treatment group 2 (15% SLO), treatment
group 3 (20% SLO), and treatment group 4
(25% SLO). To obtain dislipidemia, rats were
feeded with food rich of fat for 8 weeks,
followed by determination of MDA, LDL-C,
and HDL-C levels (pretest data). After that,
all rats were feeded with diet mix with
sardinella longiceps oil for 6 weeks for
dislipidemia and followed by determination of
MDA.
RESULTS AND DISCUSSION
Dislipidemia
Wistar rats were prepaired as
mentioned on research methods, Initial mean
of Wistar rat body weight is 49.79 if 0.77 g.
Dislipidemia. Wistar rat mean body weight
after 8 weeks intake rich of fat diet is 200.21
+ 0.51g. Mean rat body weight after SLO
intake is about 201.13 + 1.04 g.
Decrease of MDA levels of dislipidemia
‘Wistar rat
Mean MDA levels of dislipidemia
Wistar rat for pre and posttest were listed on
Table 1.
Data of mean different profile of
MDA levels for various intake of SLO after
treatment was presented on Figure 1.
Figure l
Profile of Mean Posttest MDA levels.
Anova test indicates that there is a
different of MDA levels as a results of
various SLO intake with p < 0. 05. Resume of
the results were presented on Table 4.8.
Table 2
Resume of Post Hoc of MDA Levels
Intake of various SLO results in
decrease of MDA blood levels of dislipidemia
Wistar rat. “Mean MDA blood levels can be
seen on Table 1. Mean MDA blood levels of
dislipidemiu Wistar rat cause by intake of 0%
SLO pre and posttest is about 1.25 + 0.01 and
1.22 + 0.03 um/mL. From the same table, it
can also be seen that the mean MDA bloods
levels for intake of 10%, 15%, 20%, and 25%
SLO For pre and posttest were (1.26 + 0.01)
and (0.84 + 0.13); (1.25 + 0.01) and (0.74 +
0.16); (1.26 + 0.01) and (0.61 + 0.13); (1.26 +
0.01) and (0.71 + 0.13) um/mL, respectively.
Intake of 0% SLO did not decrease MDA
blood levels compare to intake of 10% SLO.
Decrease of MDA blood levels of
dislipidemia Wistar rat were significantly
observed for intake of 10%, 15%, 20%, and
25% SLO with p < 0.05 as can be seen on
data presented on Table 2, From that table, it
can be seen that the significant highest
decrease of MDA blood levels was observed
for intake of 20% SLO, the decrease is about
0.62 um/mL.
CON CLUSSIONS AND FUTURE WORK
Conclussions
Intake of 20% SLO results in decrease
of MDA blood levels of dislipidemia Wistar
rat about 51.58%, from 1.26+0.01 to
O.61+0.l3 µm/L;
Future Work
Further research regarding of whether intake
of SLO to human results in a similar effect for
anti dislipidemia and anti inflammation need
to be carried out.
ACKNOWLEDGEMENT
The author would like to thanks Pro£
Dr. dr. A. A. G. Sudewa Djelantik, Sp.PK;
Pro£ Dr. dr. I Wayan Wita, SpJP; and Prorf
Drh. N. Mantik Astawa, Ph.D; for their
invaluable supervision during doctoral study.
Great thanks to Indonesian Government
through Higher degree of Education and
National Education Ministry for funding this
research.
REFFRENCES
Ahmed, E. 2001. Immune Mechanism in
Atherosclerosis. Dissertation. ISSBN:
91-628-4612-4.
Konferensrummet,
Centrum for Molekular Medicin,
Karolinska Sjukhuset.
Baraas-Faisa. 2006. Kardiologi Molekuler
Radikal Bebas, Disfungsi Endotel,
Aterosklerosis, Antioksidan Latihan
Fisik dan Rehabilitasi Jantung.
Bagian Kardiologi FKUI/ RS Jantung
Harapan Kita. Jakarta.
Bonetti, P. O., Lerman, L. O., and Lennan, A.
2003. Endothelial Dysfunction: A
Marker of Atherosclerosis Risk.
Aterisoscler Thromb Vase Biol. 23: p.
168-175.
Chen, G., and Goeddel, D. V. 2002. IYVFR-I
Signaling: A Beautyirl Pathway.
Science. 296: p. 1634-1635.
Coico, R, Li, S., J., and Benyamin, E. 2003.
Element of innate and acquiered
immunity
Immunology.
In:
immunology. A Short Course, 5th ed.
New Jersey, John Wiley & Sons p: 11
- 26.
Han, S. N., Leka, L. S., Lichtenstein, A.
Ausman, L. M., Schaefer, E. J., and
Meydani, S. N. 2002. Effect of
Hydrogenated and Saturated, Relative
to Polyunsaturated, Fat on Immune
and Inflammatory Responses of Adults
with Moderate Hypercholesrerolemia.
Journal of Lipid Research. 43(3): p.
445-52.
Kanjwal, M. 2004. Peripheral Aterial
Disease-The
Silent
Killer.JKPractitioner,
october-December:1
(no.4):235-232.
Simopulus, A. P. 2002. Omega-3 Fatty Acids
in Inflamation and Autoimmune
Diseasress. Journal of the American
College and Nutrition. 21 (6):p.495505.
Stefan, J., Mikko, P. S. A, Bengt, K., and JanNilsson. 1996. Human Monocytes/
Macrophages Release TNF-on in
Response
to
Ox-LDL.
Arteriosclerosis, Thrombosis, and
Vascular Biology.16:1573 -1579.
Szmitko, P. E., Wang, C. H., Weisel, R. D.,
De-Almmeida, J. R., Anderson, T. J.,
and Verma, S. 2003. New Markers of
Inflammation and Endothelial Cell
Activation. Circulation 108:19171923.
Vinay, K., Abbas, A. K., and Nelson, F. 2004.
Pathologic basic of disease 7th ed.
Elsivier ‘saunders. New York.
THROUGH DECREASE OF MDA ON RAT WISTAR
Sri Wahjuni
Biochemistry Laboratory Chemistry Departement Faculty of Math and Science University
INTRODUCTION
Nowdays, fish consumption behaviour
needs to be improve since the changes of
consumption patern to instan food. Fish,
especially from deep sea is good for health,
due to the fish rich of polyunsaturated fatty
acid (omega-3). The author want to
investigate the effect of anti dislipidemia of
Sardinella longiceps Oil (SLO) through
decrease of MDA levels.
RESEARCH METHODS
This is a true experimental study with
randomized pre and postest control group
design. This research employing 50 Wistar
rats divided into 5 groups, i.e. control group
(0% SLO), treatment group 1 (10% SLO),
treatment group 2 (15% SLO), treatment
group 3 (20% SLO), and treatment group 4
(25% SLO). To obtain dislipidemia, rats were
feeded with food rich of fat for 8 weeks,
followed by determination of MDA, LDL-C,
and HDL-C levels (pretest data). After that,
all rats were feeded with diet mix with
sardinella longiceps oil for 6 weeks for
dislipidemia and followed by determination of
MDA.
RESULTS AND DISCUSSION
Dislipidemia
Wistar rats were prepaired as
mentioned on research methods, Initial mean
of Wistar rat body weight is 49.79 if 0.77 g.
Dislipidemia. Wistar rat mean body weight
after 8 weeks intake rich of fat diet is 200.21
+ 0.51g. Mean rat body weight after SLO
intake is about 201.13 + 1.04 g.
Decrease of MDA levels of dislipidemia
‘Wistar rat
Mean MDA levels of dislipidemia
Wistar rat for pre and posttest were listed on
Table 1.
Data of mean different profile of
MDA levels for various intake of SLO after
treatment was presented on Figure 1.
Figure l
Profile of Mean Posttest MDA levels.
Anova test indicates that there is a
different of MDA levels as a results of
various SLO intake with p < 0. 05. Resume of
the results were presented on Table 4.8.
Table 2
Resume of Post Hoc of MDA Levels
Intake of various SLO results in
decrease of MDA blood levels of dislipidemia
Wistar rat. “Mean MDA blood levels can be
seen on Table 1. Mean MDA blood levels of
dislipidemiu Wistar rat cause by intake of 0%
SLO pre and posttest is about 1.25 + 0.01 and
1.22 + 0.03 um/mL. From the same table, it
can also be seen that the mean MDA bloods
levels for intake of 10%, 15%, 20%, and 25%
SLO For pre and posttest were (1.26 + 0.01)
and (0.84 + 0.13); (1.25 + 0.01) and (0.74 +
0.16); (1.26 + 0.01) and (0.61 + 0.13); (1.26 +
0.01) and (0.71 + 0.13) um/mL, respectively.
Intake of 0% SLO did not decrease MDA
blood levels compare to intake of 10% SLO.
Decrease of MDA blood levels of
dislipidemia Wistar rat were significantly
observed for intake of 10%, 15%, 20%, and
25% SLO with p < 0.05 as can be seen on
data presented on Table 2, From that table, it
can be seen that the significant highest
decrease of MDA blood levels was observed
for intake of 20% SLO, the decrease is about
0.62 um/mL.
CON CLUSSIONS AND FUTURE WORK
Conclussions
Intake of 20% SLO results in decrease
of MDA blood levels of dislipidemia Wistar
rat about 51.58%, from 1.26+0.01 to
O.61+0.l3 µm/L;
Future Work
Further research regarding of whether intake
of SLO to human results in a similar effect for
anti dislipidemia and anti inflammation need
to be carried out.
ACKNOWLEDGEMENT
The author would like to thanks Pro£
Dr. dr. A. A. G. Sudewa Djelantik, Sp.PK;
Pro£ Dr. dr. I Wayan Wita, SpJP; and Prorf
Drh. N. Mantik Astawa, Ph.D; for their
invaluable supervision during doctoral study.
Great thanks to Indonesian Government
through Higher degree of Education and
National Education Ministry for funding this
research.
REFFRENCES
Ahmed, E. 2001. Immune Mechanism in
Atherosclerosis. Dissertation. ISSBN:
91-628-4612-4.
Konferensrummet,
Centrum for Molekular Medicin,
Karolinska Sjukhuset.
Baraas-Faisa. 2006. Kardiologi Molekuler
Radikal Bebas, Disfungsi Endotel,
Aterosklerosis, Antioksidan Latihan
Fisik dan Rehabilitasi Jantung.
Bagian Kardiologi FKUI/ RS Jantung
Harapan Kita. Jakarta.
Bonetti, P. O., Lerman, L. O., and Lennan, A.
2003. Endothelial Dysfunction: A
Marker of Atherosclerosis Risk.
Aterisoscler Thromb Vase Biol. 23: p.
168-175.
Chen, G., and Goeddel, D. V. 2002. IYVFR-I
Signaling: A Beautyirl Pathway.
Science. 296: p. 1634-1635.
Coico, R, Li, S., J., and Benyamin, E. 2003.
Element of innate and acquiered
immunity
Immunology.
In:
immunology. A Short Course, 5th ed.
New Jersey, John Wiley & Sons p: 11
- 26.
Han, S. N., Leka, L. S., Lichtenstein, A.
Ausman, L. M., Schaefer, E. J., and
Meydani, S. N. 2002. Effect of
Hydrogenated and Saturated, Relative
to Polyunsaturated, Fat on Immune
and Inflammatory Responses of Adults
with Moderate Hypercholesrerolemia.
Journal of Lipid Research. 43(3): p.
445-52.
Kanjwal, M. 2004. Peripheral Aterial
Disease-The
Silent
Killer.JKPractitioner,
october-December:1
(no.4):235-232.
Simopulus, A. P. 2002. Omega-3 Fatty Acids
in Inflamation and Autoimmune
Diseasress. Journal of the American
College and Nutrition. 21 (6):p.495505.
Stefan, J., Mikko, P. S. A, Bengt, K., and JanNilsson. 1996. Human Monocytes/
Macrophages Release TNF-on in
Response
to
Ox-LDL.
Arteriosclerosis, Thrombosis, and
Vascular Biology.16:1573 -1579.
Szmitko, P. E., Wang, C. H., Weisel, R. D.,
De-Almmeida, J. R., Anderson, T. J.,
and Verma, S. 2003. New Markers of
Inflammation and Endothelial Cell
Activation. Circulation 108:19171923.
Vinay, K., Abbas, A. K., and Nelson, F. 2004.
Pathologic basic of disease 7th ed.
Elsivier ‘saunders. New York.