Cytotoxic selectivity of MJC0.3 and MJC0.5, acidic ribosome-inactivating proteins isolated from Mirabilis jalapa L. leaves against various cancer cell-lines | Retno S. Sudibyo | Journal of the Medical Sciences (Berkala ilmu Kedokteran) 4160 6849 1 SM

J Med Sci
Sismindari et al, Cytotoxic selectivity of MJC 0.3 and MJC0.5, acidic ribosome-inactivating proteins isolated
Vol. 42, No. 1, March 2010: 39-43
from Mirabilis jalapa L. leaves against various cancer cell-lines

Cytotoxic selectivity of MJC0.3 and MJC0.5,
acidic ribosome-inactivating proteins isolated
from Mirabilis jalapa L. leaves against various
cancer cell-lines
Sismindari1*, Mae Sri Hartati 2, Adhyatmika 1 , Retno S. Sudibyo
1
Faculty of Pharmacy, 2 Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

ABSTRACT
Mirabilis jalapa L. contains basic (MJ30) and acidic (MJC) Ribosome-inactivating proteins (RIPs). Further purification
of MJC has been found two RIPs, MJC0.3 and MJC 0.5. This study is aimed to prove the cytotoxic selectivity of
MJC0.3 and MJC 0.5 against many cancer cell-lines and normal cell line. The two RIPs, MJC0.3 and MJC 0.5 were tested
their cytotoxic effect on 8 human cancer cell lines and normal cell using MTT assay compared with MJC protein.
The highest cytotoxic activities of MJC0.3 and MJC0.5 were against EVSA-T followed by T47D, HeLa, WiDR, SiHa,
Raji, NS1, and MCF7, with the IC50 of 59.3, 102.4, 162.9, 190.5, 249.5, and 304.5 µg/mL, respectively for MJC0.3
and 32.8, 75.5, 86.0, 108.3, 346.7, and 220.06 µg/mL, respectively for MJC0.5 . Based on these IC50 values,

MJC0.3 and MJC 0.5 were specific to EVSA-T and T47D, whereas they were not selective against Raji and SiHa
(SI