Directory UMM :Data Elmu:jurnal:A:Animal Feed Science and Technology:Vol84.Issue3-4.May2000:
Animal Feed Science and Technology
84 (2000) 167±181
Effects of energy and protein supplementation on
microbial-N synthesis and allantoin excretion
in sheep fed guinea grass
T. Jetanaa, N. Abdullahb,*, R.A. Halimc,
S. Jalaludind, Y.W. Hoa
a
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Department of Biochemistry and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
c
Department of Agronomy and Horticulture, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
d
Department of Animal Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
b
Received 16 June 1998; received in revised form 5 March 1999; accepted 21 February 2000
Abstract
An experiment was conducted to determine the effects of two types of protein, soybean meal
(SBM) and ®sh meal (FM); and two types of energy supplements, corn ¯our (CF) and paper pulp
(PP) on in vivo digestibility of organic matter (OM), rumen fermentation pattern and dilution rate,
rumen microbial-N synthesis and ¯ow of organic matter and nitrogenous compounds through the
duodenum in four Merino rams with an average weight of 54.44.5 kg. The relationships between
duodenal purine ¯ow and urinary allantoin and duodenal protein/energy (MJ rumen VFA per day)
ratio were also investigated. The experiment was conducted in a 44 Latin square design with a
22 factorial arrangement of dietary treatments. The animals, ®tted with both rumen and duodenal
cannulae were housed in individual crates and fed chopped fresh guinea grass ad libitum twice
daily, 100 g molasses and one of the four dietary supplements: (i) 170 g FM268 g PP (FMPP);
(ii) 170 g FM268 g CF (FMCF); (iii) 200 g SBM200 g PP (SBMPP); and (iv) 200 g
SBM200 g CF (SBMCF). Each supplement, at varying rates of rumen degradability, was
formulated to provide similar amount of N and gross energy. The results showed that rumen pHs
were similar, ranging from pH 5.8 to 6.0, for all animals fed the different dietary supplements.
Rumen ammonia concentration was signi®cantly (p
84 (2000) 167±181
Effects of energy and protein supplementation on
microbial-N synthesis and allantoin excretion
in sheep fed guinea grass
T. Jetanaa, N. Abdullahb,*, R.A. Halimc,
S. Jalaludind, Y.W. Hoa
a
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Department of Biochemistry and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
c
Department of Agronomy and Horticulture, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
d
Department of Animal Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
b
Received 16 June 1998; received in revised form 5 March 1999; accepted 21 February 2000
Abstract
An experiment was conducted to determine the effects of two types of protein, soybean meal
(SBM) and ®sh meal (FM); and two types of energy supplements, corn ¯our (CF) and paper pulp
(PP) on in vivo digestibility of organic matter (OM), rumen fermentation pattern and dilution rate,
rumen microbial-N synthesis and ¯ow of organic matter and nitrogenous compounds through the
duodenum in four Merino rams with an average weight of 54.44.5 kg. The relationships between
duodenal purine ¯ow and urinary allantoin and duodenal protein/energy (MJ rumen VFA per day)
ratio were also investigated. The experiment was conducted in a 44 Latin square design with a
22 factorial arrangement of dietary treatments. The animals, ®tted with both rumen and duodenal
cannulae were housed in individual crates and fed chopped fresh guinea grass ad libitum twice
daily, 100 g molasses and one of the four dietary supplements: (i) 170 g FM268 g PP (FMPP);
(ii) 170 g FM268 g CF (FMCF); (iii) 200 g SBM200 g PP (SBMPP); and (iv) 200 g
SBM200 g CF (SBMCF). Each supplement, at varying rates of rumen degradability, was
formulated to provide similar amount of N and gross energy. The results showed that rumen pHs
were similar, ranging from pH 5.8 to 6.0, for all animals fed the different dietary supplements.
Rumen ammonia concentration was signi®cantly (p