Morphology and Biometry of Spermatozoa of the Anoa (Bubalus sp) Stained Using William's and Eosin-Nigrosin

Media Peternakan, Agustus 2010, hlm. 88-94
ISSN 0126-0472

Vol. 33 No. 2

Terakreditasi B SK Dikti No: 43/DIKTI/Kep/2008

Morfologi dan Biometri Spermatozoa Anoa (Bubalus Sp.) yang Diwarnai dengan
Pewarna William’s dan Eosin-Nigrosin
Morphology and Biometry of Spermatozoa of the Anoa (Bubalus sp) Stained Using
William’s and Eosin-Nigrosin
Yudia * #, T. L. Yusufa #, B. Purwantaraa #, M. Agila #, T. Wresdiyatib #, D. Sajuthic #, Adityaa #,
J. Manangsangd, R. Sudarwatid, & Y. T. Hastutid
a

Bagian Reproduksi dan Kebidanan, Departemen Klinik, Reproduksi dan Patologi,
Fakultas Kedokteran Hewan, Institut Pertanian Bogor
b
Bagian Histologi, Departemen Anatomi, Fisiologi dan Farmakologi,
Fakultas Kedokteran Hewan, Institut Pertanian Bogor
c

Bagian Penyakit Dalam, Departemen Klinik, Reproduksi dan Patologi,
Fakultas Kedokteran Hewan, Institut Pertanian Bogor
#
Jln. Agatis, Kampus IPB Darmaga Bogor 16680
d
PT Taman Safari Indonesia, Cisarua Bogor - Jawa Barat
(Diterima 31-07-2009; disetujui 23-07-2010)

ABSTRACT
Anoa is Indonesia endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeed, due to their agressivity, soliter, wildness and monogamous. The aim of this experiment was to observe morphology and biometry of anoa’s sperm stained using William’s (W) and
eosin-nigrosin (EN). Semen was collected from two male anoas by electroejaculator. Slides was made
for morphology and biometry observation of ejaculate. The results showed that abnormal sperm
morphology of the mature anoa (10 years) was 32.27%-35.00%, meanwhile the young one (3 years)
was 24.03%–31.89%. In mature anoa, measurement for head width, and midpiece and tail length of
sperm were not signficantly different between W and EN staining, but significantly different (P