Direct counting and detection of living Salmonella in biological fluid

Suppl

I - 1998

Diagnostic

Direct counting and detection of living
Salmonella in biological fluid

49

s3-3

Takayuki Ezaki, Kenji Hirose, Li-Cheng Zhao, Khan Abdul Quayum, Masaki Miyake, Yoshiaki Kawamura

Abstrak
Untuk mengembangkan metoda cepat untuk menghilung Salmonella hidup dalam cairan biologis, 4 metoda tletel106 cfu/ml

<

t

hr

Visualization of live
and dead cells

>100

cfu/ml t hr - 2 hr

VIM Camera
Light Cyder

hi,

>5000 cfu/ml

Sænning Microsæpy
Photon Counting

teritidis, 15 strains of

Time
required

number
Single Cell

also analiyzed.

+

Comparison of 4 different counting methods

ND

ND

ND

ND: no data

ND
ND
ND
ND
-+
ND

+

Paratyphi

B

Tâble

+

A

394EG/395F

A

+
ND
ND
ND
ND

C primers

FCltFC2

Serovar

Gene

Group A

strain.s

Detection of

Salmonella

Salmonella

* Clinical

3.

Sensitive, High background

>10

cfu/ml

< 30

min.

Sensitive, Real

ïme

Monitor of Ampliæns

52

Typhoid Fever and other Salmonellosis

Med J Indones

Promotor

Suppressor

friB

Pro/Operator

ftic

rh1

HlRepressor protein

t
Phase 2
Flagellin

r+> 500
FC1 +>
fllc394E rf>
fliO384O

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