S KIM 1005280 Table of content

DAFTAR ISI
HALAMAN JUDUL
LEMBAR PENGESAHAN
LEMBAR PERNYATAAN ..................................................................................... i
ABSTRAK ................................................................................................................ ii
KATA PENGANTAR .............................................................................................. iv
UCAPAN TERIMA KASIH ................................................................................... v
DAFTAR ISI .............................................................................................................vii
DAFTAR GAMBAR ................................................................................................ x
DAFTAR TABEL .................................................................................................... xi
DAFTAR LAMPIRAN ............................................................................................xii
BAB I

PENDAHULUAN ............................................................................... 1

1.1

Latar Belakang ...................................................................................... 1

1.2

Rumusan Masalah ................................................................................. 3

1.3

Tujuan Penelitian .................................................................................. 4

1.4

Batasan Masalah Penelitian .................................................................. 4

1.5

Manfaat Penelitian ................................................................................ 4

1.6

Struktur Organisasi Skripsi ................................................................... 5

BAB II

TINJAUAN PUSTAKA ...................................................................... 6

2.1

Parkinson .............................................................................................. 6

2.2

Landep .................................................................................................. 8

2.3

Ekstraksi................................................................................................12

2.4

Skrining Fitokimia ................................................................................13

2.4.1

Alkaloid ................................................................................................13

2.4.2

Tanin .....................................................................................................14

2.4.3

Saponin .................................................................................................14

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

2.4.4

Terpenoid ..............................................................................................14

2.4.5

Flavonoid ..............................................................................................16

2.5

Spektoskopi Fourier Transform Infra Red (FTIR) ...............................16

2.6

Uji Farmakologi ....................................................................................17

2.6.1

Hewan Uji .............................................................................................17

2.6.2

Pengujian Toksisitas .............................................................................18

2.6.3

Katalepsi ...............................................................................................19

BAB III

METODE PENELITIAN ..................................................................22

3.1

Sampel dan Lokasi Penelitian ...............................................................22

3.2

Alat dan Bahan Penelitian.....................................................................22

3.2.1

Alat........................................................................................................22

3.2.2

Bahan ....................................................................................................22

3.3

Metode Penelitian .................................................................................23

3.3.1

Proses Ekstraksi ....................................................................................24

3.3.2

Skrining Fitokimia ................................................................................24

3.3.3

Karakterisasi Gugus Fungsi ..................................................................25

3.3.4

Uji Farmakologi Antiparkinson ............................................................25

3.3.4.1 Preparasi Hewan Uji .............................................................................25
3.3.4.2 Preparasi Pemberian Dosis ...................................................................26
3.3.4.3 Pengujian Toksisitas .............................................................................27
3.3.4.4 Pengujian Katalepsi ..............................................................................27
3.3.4.5 Analisis Data .........................................................................................28
BAB IV

HASIL DAN PEMBAHASAN ...........................................................29

4.1

Hasil Ekstraksi Akar Landep ................................................................29

4.2

Hasil Skrining Fitokimia .......................................................................30

4.3

Hasil Karakterisasi FTIR ......................................................................31

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

4.2

Hasil Uji Toksisitas Akut ......................................................................32

4.1

Hasil Uji Katalepsi ................................................................................33

BAB V

KESIMPULAN DAN SARAN ...........................................................39

5.1

Kesimpulan ...........................................................................................39

5.2

Saran .....................................................................................................36

DAFTAR PUSTAKA ...............................................................................................40
LAMPIRAN ..............................................................................................................43

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

DAFTAR GAMBAR

Gambar 2.1. Struktur Dopamin

6

Gambar 2.2. a. Struktur Levodopa

7

b. Struktur Amantadin

7

c. Struktur Karbidopa

7

d. Struktur Enthocapone

7

Gambar 2.3. Landep

8

Gambar 2.4. a. Daun Landep

9

b. Akar Landep

9

c. Batang Landep

9

Gambar 2.5. Struktur senyawa yang terkandung dalam landep

10

Gambar 2.6. Reaksi uji fitokimia alkaloid

14

Gambar 2.7. Reaksi uji fitokimia triterpenoid

15

Gambar 2.8. Reaksi uji fitokimia flavonoid

16

Gambar 2.9. Struktur Haloperidol

20

Gambar 3.1. Bagan Alir Penelitian

23

Gambar 3.2. Skema Pengujian Katalepsi

28

Gambar 4.1. Akar landep

29

Gambar 4.2. Ekstrak Kering Akar Landep

30

Gambar 4.3. Spektrum IR Akar Landep

31

Gambar 4.4. Pengujian toksisitas akut

33

Gambar 4.5. a. Pengelompokan Mencit

34

b. Induksi Haloperidol

34

c. Pengujian Katalepsi

34

Gambar 4.6. Grafik Uji Katalepsi Ekstrak Landep Dosis

36

200 mg/kg dan 400 mg/kg

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

DAFTAR TABEL

Tabel 2.1. Klasifikasi ilmiah landep (Barleria prionitis)

8

Tabel 2.2. Klasifikasi Mencit

18

Tabel 2.3. Tingkat Toksisitas dosis suatu bahan

19

Tabel 4.1. Hasil uji Skrining Fitokimia ekstrak akar landep

30

Tabel 4.2. Hasil pengujian tingkat toksisitas akut

33

Tabel 4.3. Data Hasil uji katalepsi

35

Tabel 4.4. Hasil Uji One way ANOVA Uji Dunnet Ekstrak Akar
Landep Dosis 200 mg/kg Berat Badan dan Dosis 400 mg/kg
Berat Badan Terhadap Pengujian Katalepsi.

37

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu

DAFTAR LAMPIRAN

Lampiran 1. Perhitungan Persentase Ekstrak dalam Akar landep.

43

Lampiran 2. Perhitungan pembuatan sediaan haloperidol.

44

Lampiran 3. Konversi Perhitungan dosis untuk berbagai jenis hewan
dan manusia.

45

Lampiran 4. Data berat badan mencit pada pengujian tingkat
toksisitas akut

46

Lampiran 5. Data berat badan mencit pada pengujian katalepsi

47

Lampiran 6. Prosedur pengujian katalepsi

48

Lampiran 7. Tes Kenormalan

49

Lampiran 8. Data hasil uji statistik

50

Lampiran 9. Foto-foto kegiatan

54

Ari Ardianti, 2014
Uji Farmakologi Ekstrak Akar Landep (Barleria Prionitis Linn) Asal Indonesia Sebagai
Antiparkinson
Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu