M01653
Refleksi 10 tahun Magister Biologi
Universitas Kristen Satya Wacana
Venny Santosa, Ph.D
Kamis, 15 Januari 2015
Fakultas Biologi UKSW
Class of 1998 (1998-2002)
Venny Santosa
Perwakilan alumni MB
~2006 worked in consumer goods companies
Double-degree program
Magister Biologi UKSW
Class of 2006 (September 2006-Augusts 2007)
Kwansei Gakuin University, Kobe, Japan
September 2007-March 2009
Kwansei Gakuin University, Kobe, Japan
April 2009-March 2010
Kwansei Gakuin University, Kobe, Japan
April 2010-March 2013
Kwansei Gakuin University, Kobe, Japan
April 2013-September 2013
Family life
On scholarship
= FREE education
Research and
publication
Academic community
Traveling!!!
Social life
Determination of research topics and goal
Browsing of published data
Idea !
Research designs; hypothesis
Action!
Progress evaluation and adjustment/alteration Time management
Data analysis and peer review
Paper draft writing and revision, grammar check
Publication : paper and seminar
Application
Overview of Tanaka Lab Laboratory
Science and Technology Fac., Bioscience Dept.
Kwansei Gakuin University
Members: 1 Prof, 1 secretary, 8-15 students (undergraduate-postgraduate), PD
Working hours: 9-17 (official), Mon-Fri (official)
Weekly seminar: progress report and journal club
Seminar with other lab: 1-2x /week
Discussion : 1-2x/week
Task per month (every member):
2x progress report (every 2 weeks), there has to be NEW DATA
1 journal club (3-4 papers)
1 seminar with other lab
Discussion + Additional seminar(s) and class(es)
Japanese working ethics
Tanaka Sensei’s working hours:
Mon-Fri : 8.30 – 19.00/19.30 + 23.00 – 2.00
Sat (Sun): 8.30 – 17.00
Time to come to work
Start to work
Working style
Time to go home
Go home early
Hardwork
Social pressure
Ideology
Work on weekend
Death from overwork
Attitude
early
on time
hectic
late, work long hours
embarrassed
appreciated, admired
high
live to work'
normal
working long hours = sign of diligence and hardworking attitude
idle person = useless and incompetence person
It is usual for students to come earlier and go home later than the professor
Sample of experimental design (I)
mating
Conjugation
Mei S phase
Crossing/mating (day 1) 5 min/plate
Mon
Fri
Incubation period (1-2 day, check daily)
Meiosis I
Meiosis II
Sporulation
Pick 25-40 mature sacks/plate (0.5->1 h/plate) Tue/Wed
Incubation period (overnight)
Separate spores (0.5->1 h/plate)
The meiotic cycle of
Schizosaccharomyces pombe
Wed/Thu
Sat
Sun
3-5 plates
Sample of experimental design (II)
The DNA damage by heat in temperature-sensitive mutants
Grow cells in 25C (2 strains)
25C
34C 6h
mcb1
Take YFP photos with fluorescence microscope
Move to 34C
mcb1L254P
Incubate for 6h
Take YFP photos
•Count cells with 1 focus and 2/more foci
•At least 200 cells/experiment
%cells with Rad22-YFP signal
•Repeat experiment 3x
70
60
1 focus
2/more foci
50
40
30
20
10
0
mcb1 mcb1L254P mcb1L363P
25C
mcb1 mcb1L254P mcb1L363P
34C 6h
2 strains x 2 temperatures x 3 repeats x 200 cells/experiment = 2400 cells
x 2 experiments = 4800 cells
Academic community
•Professor and Lab members (junior and senior, PD, secretary)
•Other professor and their lab members in the same research field
•Neighboring labs
•University staffs
•Other students
Social life
Love
•Seminar
•Invited presentation
•Lab trip
•Research at other lab
•Field research
Universitas Kristen Satya Wacana
Venny Santosa, Ph.D
Kamis, 15 Januari 2015
Fakultas Biologi UKSW
Class of 1998 (1998-2002)
Venny Santosa
Perwakilan alumni MB
~2006 worked in consumer goods companies
Double-degree program
Magister Biologi UKSW
Class of 2006 (September 2006-Augusts 2007)
Kwansei Gakuin University, Kobe, Japan
September 2007-March 2009
Kwansei Gakuin University, Kobe, Japan
April 2009-March 2010
Kwansei Gakuin University, Kobe, Japan
April 2010-March 2013
Kwansei Gakuin University, Kobe, Japan
April 2013-September 2013
Family life
On scholarship
= FREE education
Research and
publication
Academic community
Traveling!!!
Social life
Determination of research topics and goal
Browsing of published data
Idea !
Research designs; hypothesis
Action!
Progress evaluation and adjustment/alteration Time management
Data analysis and peer review
Paper draft writing and revision, grammar check
Publication : paper and seminar
Application
Overview of Tanaka Lab Laboratory
Science and Technology Fac., Bioscience Dept.
Kwansei Gakuin University
Members: 1 Prof, 1 secretary, 8-15 students (undergraduate-postgraduate), PD
Working hours: 9-17 (official), Mon-Fri (official)
Weekly seminar: progress report and journal club
Seminar with other lab: 1-2x /week
Discussion : 1-2x/week
Task per month (every member):
2x progress report (every 2 weeks), there has to be NEW DATA
1 journal club (3-4 papers)
1 seminar with other lab
Discussion + Additional seminar(s) and class(es)
Japanese working ethics
Tanaka Sensei’s working hours:
Mon-Fri : 8.30 – 19.00/19.30 + 23.00 – 2.00
Sat (Sun): 8.30 – 17.00
Time to come to work
Start to work
Working style
Time to go home
Go home early
Hardwork
Social pressure
Ideology
Work on weekend
Death from overwork
Attitude
early
on time
hectic
late, work long hours
embarrassed
appreciated, admired
high
live to work'
normal
working long hours = sign of diligence and hardworking attitude
idle person = useless and incompetence person
It is usual for students to come earlier and go home later than the professor
Sample of experimental design (I)
mating
Conjugation
Mei S phase
Crossing/mating (day 1) 5 min/plate
Mon
Fri
Incubation period (1-2 day, check daily)
Meiosis I
Meiosis II
Sporulation
Pick 25-40 mature sacks/plate (0.5->1 h/plate) Tue/Wed
Incubation period (overnight)
Separate spores (0.5->1 h/plate)
The meiotic cycle of
Schizosaccharomyces pombe
Wed/Thu
Sat
Sun
3-5 plates
Sample of experimental design (II)
The DNA damage by heat in temperature-sensitive mutants
Grow cells in 25C (2 strains)
25C
34C 6h
mcb1
Take YFP photos with fluorescence microscope
Move to 34C
mcb1L254P
Incubate for 6h
Take YFP photos
•Count cells with 1 focus and 2/more foci
•At least 200 cells/experiment
%cells with Rad22-YFP signal
•Repeat experiment 3x
70
60
1 focus
2/more foci
50
40
30
20
10
0
mcb1 mcb1L254P mcb1L363P
25C
mcb1 mcb1L254P mcb1L363P
34C 6h
2 strains x 2 temperatures x 3 repeats x 200 cells/experiment = 2400 cells
x 2 experiments = 4800 cells
Academic community
•Professor and Lab members (junior and senior, PD, secretary)
•Other professor and their lab members in the same research field
•Neighboring labs
•University staffs
•Other students
Social life
Love
•Seminar
•Invited presentation
•Lab trip
•Research at other lab
•Field research