SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE WHITE RATS KIDNEY NECROSIS Repository - UNAIR REPOSITORY
FOLIA MEDIC,A
A
INDONESIAN
March 201 6
Vol. 52 No. 1 lanualY
sIR CODIS (CSf1PO, THOI, TPo)! vWA) LOCI
iniriJirJulG,
GENETIC VARIATIoN AIIALYSIS
It{
MADURESE
agung Sosiawan, Nola Marsaret)
ml***tm*,qf
Lq.5f l'fipi,trfliptl!11'n{ALsuPPREssroN
REPERFUSION
ON GLUCOSE LEVELS DURII{G TSCHEMIA
THE ACUTE EFFECT OF ERYTHROPOIETIN
George zografos, APostolos Papalois)
consBntinos noutis, Konstantinos Toutouzas,
C VIRUS COINFECTTO IT{ TB DEVELOPMENT
ROLE OF HEPATMS B AND HEPATITS
Rahmatullah, Dwi susanti)
i".tuii
',HX[H[$npos,
r*"
iiioli
SHOCX
PROrr
TO EXPRESSTOT{
coRRElrTIOl{ BETWEET{ IIITRACELLULAR HEAT
iir rrsopxrnvlcEAt caRcll{oitA
AND cERvIcrL LYupx noots Ei-riitlilln
AH;;d R"riti;i, sri Herawati, Elida Mustikaninstvas)
.,ff .&",;,ff
m?eg{!i,K!#Tilt^,?;",t$lLti'.li?-Bf*.rEi.1f'|l"$wrHFAcroR-A
RArs wrrH anersr crxcen
iiEi#;ili wHIrEI Ketut
Sudiana)
io*
Cvn'ttti, Dewi,
ier,,
effi;il;'nglihl
USE IN CHILDR"EN WITH PEDIATRIC
ANALYSIS OF DEFERASIROX AND DEFERIPRO
B-THALASSEUIA MA'OR
s'n"tj*o, Bambans sudarmanto)
EFFECT OF BODY TIIASS TNDEX
6F HEARr FArLuRe
ii
(BMI) ON MORTALITY AI{O READMISSION
prnerr= iii din6i6irccur-m ourPArrElr
cARE
ultrr
iii.3bnouo llosPrrAl, SURABAYA
iirri
niztia r-estarl, Andlianto, J Nugroho)
AI{D. EARLY INTERVENTION
IMPIIT4 EIITATIOT{ ()1{ STI'itUI-ATION' DETECTION'
MoJo'
pnoener
IN-PusKEsnAs
;Ever-opxrm'ii'bI-oiij
ei'offi-'iiil
il;ftiil;iiiliiiiasrzr
Flbrenuna Sustini)
br
OF C}IILD
GRowrH ScREENTNG
(Dwi Susanu,
SURABAYA
REPORT IT{ DR' SOETOMO HOSPTTAL
SIX YEARS COCHLEAR IMPIAITT PROGRAT4
(Haris M Ekorini)
EXTRACT
SHORT.TERITI TOECTTY TEST OF SII{GALAWANG
wxrrr mrs noNEY
&;i.ffi;tff
O
MAIE
ECROSTS
r'riiu"r'o,
*itt
Mustika' Annv setiio Rahaju)
IN PERCUTAT{EOUS
INTRAPROCEDURAL STENT THROMBOSIS
CORONARY AI{GIOPTASTY
(Yudi Her Oktaviono)
TRAI{SAI{AL ENDORECTAL PULL-THROUGH'
SECONDARY IIPAROSCOPIC ASSTSSTED
A PRE.EUi4INARY STUDY
(Barmadisatrio, Hariono)
Intemauonal Online Dlstribution by ProQuestn
www.Proguest.com
FOLIA MEDICA INDONESIANA
rssN 2355-8393
Vol. 52 no. l January March 2015
Medlcal tournal, publlshed by Alrlangga Unhrerdty Sdrool of M€didne, Surabaya, publbtlng
orlglnat'bast m&lcal and dinlcal arddec presented as r€seatdt at'Udcs and revl* artides
Editor-h Chief
ProfDr Suhartono Taat Puta, dr' MS
Deputy Editor-in-Chief
I Dewa Gede Ugrasena clr, SPA(K)
Editoriel Boerd
ProfBambang Permono' dr, SPA(K)
Prof Purnomo SuryohudoYo, dl
Doddy M Soebadi, dr, SpB, SpU(K)
Dr
Prof
Prof Soebartono DS, &, SPOC(K)
ProfDr Hadanto' dr' AIF
Dr FM Juclajan4 dr, SPPK
Prof SoetiPto, dr, MS, PhD
Prof Dr Suharto, dr, MSc, DTM&H, SpPD-KTI
hof Dr Subijanto Marto Soedarmo, dr, SpA(K)
Prof Dr Krmtaman, dr, MS, SPMK
Dr Anang Endaryanto, dr, SPA(K)
Edhi Rianio, &' MS
Manrging Editor
Ahmad Suryawan, dr, SPA(K)
Dr I Ketut Sudiana, drs, MSi
Dr Nvrlo Pumami' dr, SPTHT-KL(K)
Viskasari Pintoko Katanjati, dr, MKes, PhD
Purwo Sri Rejeki, dr, MKes
Dr Gondo Mastutilq &h' MKes
Mia Ratwita Andarsini' dr' SPA
Wita Saraswati, dr, SPOG
Dr Ahmad Yudianto' dr, SPF
Linda Dewanti, dr, MKes, HMsc' PhD
Secrctrriat
Mochamrnad ZuhdY' S'Sos
FitriYa Niken Ariningsih' S'Sos
AthfiYatul Fatati' S Ant
Published bY
(Grahr Masyarikat Ilmieh Kedokteren)
(Center for Medicel Science Communit!)
: GRAMIK
March' April-June' Jutv-September' october-December)
UniYersity School of Medicine
l;;;;iJ-r*
Address
'
iirt"ogg,
60 I 3 I
Jl. Prof dr Moestopo 47 Surabaya
ext l35
Phone: 62-31-5013749, 5020251-3
62-31-50224'12
49,
t-5013'l
Fax : 62-3
E-mait: foliamedica@ gmail'com
Accredited Bo. 2/E/KPT/201
5
FOLIA MEDICA INDONESIANA
ISSN 2355-8393
Vol. 52 no.
l
January - March 2016
CONTENTS
1-6
Ahmad Yudianto, Agung Sosiawan, Nola Margaret)
'TRCODIS(CSF,IPO,THOI,TPOX,VWA)LOCIGENETICVARIATIONANALYSISINMADURESE
\NALYSISOFINOUCTIONPHASEGLUCOCORTICOIDUSEONADRENALSUPPRESSION
N PEDIATRIC PATIENTS WITH ACUTE LYMPHOBLASTIC LEUKEMIA
Octaviana Simbolon, Yulistiani, IDG Ugrasena, Mariyatul Oibtiyah)
7
ISCHEMIA REPERFUSION
rHE ACUTE EFFECT OF ERYTHROPOIETIN ON GLUCOSE LEVELS DURING
NJURY IN RATS
Apostolos Papalois)
Constantinos Tsompos, Constantinos Panoulis, Konstantinos Toutouzas' George Zografos,
1OLE OF HEPATITIS B ANO HEPATITS C VIRUS COINFECTION IN TB DEVELOPMENT
Pudji Lestari, lvan Rahmatullah, Dwi Susanti)
'.13
t4-18
19-23
]oRRELATIoNBETWEENINTRACELLULARHEATSHocKPRoTEINT0ExPRESSIoN
rru6 cinvrcar- r-vMpH NoDEs ENLARGEMENT lN NASoPHARYNGEAL cARclNoMA
24-34
Achmad C Romdhoni, Sri Herawati, Elida Mustikaningtyas)
OF
:FFECT oF cayratia trifotia L (Domin) EXTRACT oN EEotlgE-P lllREssloN
FACToR
GROwTH
ENDoTHELIAL
vlscuLAR
vrarnixrrrerl-lOpnorrlr'rese-s (Mfue-s1 aHo
,VEGF-A)
CANCER
BREAST
WITH
RATS
IN WHITE
Devi Cynthia Dewi, lKetut Sudiana)
-
-A
35-41
qNALYSISoFDEFERASIRoxANDDEFERIPRoNUSEINcHILoRENWITHPEoIATRIc
].THALASSEMIA MAJOR
'Evy Sari Sutrisnaningsih. Suha4ono. Bambang Sudarmanto)
42-46
EFFECT OF BODY MASS INDEX (BMI) ON MORTALITY AND READMISSION
ci iiani
mruunE PATIENTs lN cAnotovescut-rR ourPATlENr cARE uNlr
AT DR. SOETOMO HOSPITAL, SURABAYA
:Sella Rizkita Lestari, Andrianto' J Nugroho)
47-50
EARLY INTERVENTION OF CHILD
IMPLEMENTATION ON STIMULATION' DETECTION, AND
dio-wrx eNo
oevELoPMENT (sDlDTK) PRocRAM lN PUSKESMAS MoJo'
iunaelva srtuL EMPHASIzE
oN GRowrH ScREENING
(Dwi Susanti, Florentina Sustini)
IN DR. SOETOMO HOSPITAL SURABAYA
SIX YEARS COCHLEAR IMPLANT PROGRAM REPORT
(Haris
N,l
Ekorini)
ON MALE
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT
NECROSIS
WHITE RATS KIDNEY
n"rnonrn9"n Naibaho' Arifa Mustika, Anny Setijo Rahaiu)
5l -56
57-61
52-65
lnrJy
INTRAPROCEDURAL STENT THROMBOSIS IN PERCUTANEOUS
CORONARY ANGIOPLASTY
(Yudi Her Oktaviono)
ENDORECTAL PULL'THROUGH'
SECONDARY LAPAROSCOPIC ASSISSTED TRANSANAL
A PRE.ELIMINARY STUOY
(Barmadisatrio, Hariono)
66-73
74-79
:
Folia Medica Indonesiana Vol. 52 No. I January - March 2016 : 62-65
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE
WHITE RATS KIDNEY NECROSIS
Ardy Hamonangan Naibahor, Ari[a Mustika!, Anny Setijo Rahaju]
rMedical Doctor Program, rDepanment of Medical Pharmacology.
rDepartment ofAnatomic Pathology, Faculty of Medicine,
Airlangga University, Surabaya
ABSTRAK
Daun Singawalang digunakan masyarakat sebagai obat tradisiohal penderita pneumonia akibat penyakit TBC. Upaya penelitian
bidang bitani naipui farmakologi terhadap daun singawalang (Petiveria alliaceae) sudah banyak dilakukan. Beberapa penelitian
bertuiuan mengetaiui khasiat daun tersebut sudah dilakukan, namun penelilian unluk mengelaltui keamanan penggunaannya masih
ini merupakan
iaraig. Peneliian ini be ujuan untuk mengetahui toksisitas jangka pendek ekstrok daun Singalawang. Penelitian
-jenis-ldboratorium
eksperimental dengan rancangan penelitian lhe posl - lest only conlrol group design. Metode pengambilan
'sampel
iolah simple rindom sampling, mengguhakon sanpel 32 ekor mencil (Mus musculus) jailan sebagai hewan coba dengan
berir badan rata-rata 20 gram dan berumur kurang lebih 2 bulan. Hewan percobaan dibagi ke dalam 4 (empa) kelompok, masingmasing kelompok terdiri dari 8 (delapan) ekor mencit yaitu, kelompok kontrol (P0) mendapat aquadest sebagai Pelarut bahan.
Keloipok peitakuan I (Pl) mendapatkan ekstak etanol daun singawalang (Petiveria alliaceae) dosis rendah yaitu 91mg/kgbb.
Kelompok'perlakuan 2 (P2) nendapatkan ekstrak etanol daun singawalang (Petiveria alliaceae) dosis sedang yaitu |80ng/kgbb.
Kelompok-perlokuan 3 (P3) nendapatkan ekstrak etanol daun singawalang (Petiveria allidceae) dosis tinggi yaitu 360mg/kgbb.
Ekst;k et;nol diberikah secara peroral mengguhakan sonde sehari sekali seloma 30 hari. Hasil penelitian ini menunjukkan bahwa
ektrak etanol daun singau,alang (Petiveria alliaceae) ini ti
of
short-term (sub-acute) toxicity tests on
singowalang (Petiveria alliaceae) leaf extract on
necrosis of the kidneys of male white mice (Mas
musculus\ for 30 days showed that the leaf extract of
singawalong (Petiveria alliaceoe) from Batu, East Java,
showed that it did not induce renal necrosis in doses of
90 mg/kgbw, 180 mg/kgbw and 360 mg/kgbw.
DISCUSSION
caused by exposure lo sinSowalang leaf extract. Murine
renal tubular necrosis was found in 8th experimental
animal
Results of sub-acute toxicity test of the leaves
singawalang (Petiveria olliaceae) in the kidney
of male white mice (Mus musculus)
Results
The observations in the control group (P0) receiving
aquadest I ml/day for 30 days did not cause kidney
necrosis. Kidney necrosis on 8th rePlication was not
'7
l.
Short-term toxicity tests on extmct of singa-walang
(Petiveria alliaceae) white to the kidney of male white
6Yo. The mouse was the one that was
mice (Mus musculus) revealed not to caus€ necrosis
found dead on the morning ofthe I I th day, so it was not
the subiect of the experimental animals that was taken
into account. Observations in group I (Pl) singawalang
leaf extract (Petiveria alliaceae) 90 mglkg in mice
(0-269 gok) I ml/day for 30 days did not cause kidney
necrosis on the experimental animals.
of
the kidneys. The results of this study were similar to
those found by Zakaria in 2010, which proYed that the
ethanol extract ofthe leaves do not have acut€ toxicity.
Toxic effect can be reversible and ineversible. Toxic
effect can be reversed when the body is exposed to low
levels or for a short time, while ineversible effects can
be generated from exposure to higher levels or for a
long time (Lu, 1995). Therefore, in elevated level and
extended test time in chronic toxicity test irreversible
toxic efI€cts might be generated, which in this regard
in group 2 (P2) receiving singowalang
leaf extmct (Petiveria alliaceae) 180 mg/kg in mice
(0.528 go/.) I ml/day for 30 days did not cause kidney
necrosis on experimental animals. The observation of
group 3 (P3) Award singawolang leaf extact (Petiveria alliaceae) 360 mg^g in mice (1.056 {/.) | mllday
for 30 days also did not cause kidney necrosis on the
Observations
was necrosis.
experimental animals.
64
Short-Term Toxiciry Test ofSingalawang Extract on Kidney Necrosis (Ardy Hamonangan Naibaho, Arifa Mustika, Anny Selijo
resutt
Functional elfects usually reversible, whereas the
morphological e(fects usually ineversible. Several
studies have been conducted to find functional changes
that can be identified in advance, or can be detected in
animals in lower doses than the dose that causes
of
R haiu)
shon-term toxicity test (sub-acute) of
singawalang (Petiveria alliaceae) leafextract on kidney
necrosis of male white mice (Mus muscalus) for 30 days
showed that singawalang (Petiverid alliaceae) the leaf
extract is not nephrotoxic.
morphological changes. Evidence gathered from liYer
and kidney tests seemed not supporting this notion.
REFERENCES
However, recent discoveries showed that functional test
may be more sensitive. In addition, functional testing is
valuable for the continued monitoring oftoxic effects on
target organs in the long-term studies in animals and
humans (Lu, 1995). The present study does not address
Lien, Y-HH, et aI.2006. Kidney [nternational: SlPlSelective Agonist, SEW287 t, Ameliorates Ischemic
Acute Renal Failure. NPG. USA
needed further research.
Lu, Frank C. 1995. Basic Toxicology: Fundamentals,
Target Organs, and Risk Assesment. Hemisphere
CONCLUSION
Publishing Corporation. New York. Page: 46-65
Sarman. 2008. Singawalang, Si Tangguh Melawan
TBC. Accessed: 25 llly 2012. Cited from: http://
the toxicity
of ethanol extract on organ function
that
omnilogos. blogspot.com,/2008ll2lsingawalang-sitangguh-melawan-tbc.html
7akaia.2010. Uji Toksisitas Akut Daun Singawalang
pada Mencit Putih Jantan Laporan Penelitian.
of
leaves .tlTgalralar?g does not
cause death if exposed once and the reaction is awaited
for 24 hours in doses of 90 mg/kg bw, 180 mg/kg bw,
The ethanol extmct
360 mg/kg bw. This proves that the extract of
Surabaya
singowalang leaves does not have acute toxicity. The
65
A
INDONESIAN
March 201 6
Vol. 52 No. 1 lanualY
sIR CODIS (CSf1PO, THOI, TPo)! vWA) LOCI
iniriJirJulG,
GENETIC VARIATIoN AIIALYSIS
It{
MADURESE
agung Sosiawan, Nola Marsaret)
ml***tm*,qf
Lq.5f l'fipi,trfliptl!11'n{ALsuPPREssroN
REPERFUSION
ON GLUCOSE LEVELS DURII{G TSCHEMIA
THE ACUTE EFFECT OF ERYTHROPOIETIN
George zografos, APostolos Papalois)
consBntinos noutis, Konstantinos Toutouzas,
C VIRUS COINFECTTO IT{ TB DEVELOPMENT
ROLE OF HEPATMS B AND HEPATITS
Rahmatullah, Dwi susanti)
i".tuii
',HX[H[$npos,
r*"
iiioli
SHOCX
PROrr
TO EXPRESSTOT{
coRRElrTIOl{ BETWEET{ IIITRACELLULAR HEAT
iir rrsopxrnvlcEAt caRcll{oitA
AND cERvIcrL LYupx noots Ei-riitlilln
AH;;d R"riti;i, sri Herawati, Elida Mustikaninstvas)
.,ff .&",;,ff
m?eg{!i,K!#Tilt^,?;",t$lLti'.li?-Bf*.rEi.1f'|l"$wrHFAcroR-A
RArs wrrH anersr crxcen
iiEi#;ili wHIrEI Ketut
Sudiana)
io*
Cvn'ttti, Dewi,
ier,,
effi;il;'nglihl
USE IN CHILDR"EN WITH PEDIATRIC
ANALYSIS OF DEFERASIROX AND DEFERIPRO
B-THALASSEUIA MA'OR
s'n"tj*o, Bambans sudarmanto)
EFFECT OF BODY TIIASS TNDEX
6F HEARr FArLuRe
ii
(BMI) ON MORTALITY AI{O READMISSION
prnerr= iii din6i6irccur-m ourPArrElr
cARE
ultrr
iii.3bnouo llosPrrAl, SURABAYA
iirri
niztia r-estarl, Andlianto, J Nugroho)
AI{D. EARLY INTERVENTION
IMPIIT4 EIITATIOT{ ()1{ STI'itUI-ATION' DETECTION'
MoJo'
pnoener
IN-PusKEsnAs
;Ever-opxrm'ii'bI-oiij
ei'offi-'iiil
il;ftiil;iiiliiiiasrzr
Flbrenuna Sustini)
br
OF C}IILD
GRowrH ScREENTNG
(Dwi Susanu,
SURABAYA
REPORT IT{ DR' SOETOMO HOSPTTAL
SIX YEARS COCHLEAR IMPIAITT PROGRAT4
(Haris M Ekorini)
EXTRACT
SHORT.TERITI TOECTTY TEST OF SII{GALAWANG
wxrrr mrs noNEY
&;i.ffi;tff
O
MAIE
ECROSTS
r'riiu"r'o,
*itt
Mustika' Annv setiio Rahaju)
IN PERCUTAT{EOUS
INTRAPROCEDURAL STENT THROMBOSIS
CORONARY AI{GIOPTASTY
(Yudi Her Oktaviono)
TRAI{SAI{AL ENDORECTAL PULL-THROUGH'
SECONDARY IIPAROSCOPIC ASSTSSTED
A PRE.EUi4INARY STUDY
(Barmadisatrio, Hariono)
Intemauonal Online Dlstribution by ProQuestn
www.Proguest.com
FOLIA MEDICA INDONESIANA
rssN 2355-8393
Vol. 52 no. l January March 2015
Medlcal tournal, publlshed by Alrlangga Unhrerdty Sdrool of M€didne, Surabaya, publbtlng
orlglnat'bast m&lcal and dinlcal arddec presented as r€seatdt at'Udcs and revl* artides
Editor-h Chief
ProfDr Suhartono Taat Puta, dr' MS
Deputy Editor-in-Chief
I Dewa Gede Ugrasena clr, SPA(K)
Editoriel Boerd
ProfBambang Permono' dr, SPA(K)
Prof Purnomo SuryohudoYo, dl
Doddy M Soebadi, dr, SpB, SpU(K)
Dr
Prof
Prof Soebartono DS, &, SPOC(K)
ProfDr Hadanto' dr' AIF
Dr FM Juclajan4 dr, SPPK
Prof SoetiPto, dr, MS, PhD
Prof Dr Suharto, dr, MSc, DTM&H, SpPD-KTI
hof Dr Subijanto Marto Soedarmo, dr, SpA(K)
Prof Dr Krmtaman, dr, MS, SPMK
Dr Anang Endaryanto, dr, SPA(K)
Edhi Rianio, &' MS
Manrging Editor
Ahmad Suryawan, dr, SPA(K)
Dr I Ketut Sudiana, drs, MSi
Dr Nvrlo Pumami' dr, SPTHT-KL(K)
Viskasari Pintoko Katanjati, dr, MKes, PhD
Purwo Sri Rejeki, dr, MKes
Dr Gondo Mastutilq &h' MKes
Mia Ratwita Andarsini' dr' SPA
Wita Saraswati, dr, SPOG
Dr Ahmad Yudianto' dr, SPF
Linda Dewanti, dr, MKes, HMsc' PhD
Secrctrriat
Mochamrnad ZuhdY' S'Sos
FitriYa Niken Ariningsih' S'Sos
AthfiYatul Fatati' S Ant
Published bY
(Grahr Masyarikat Ilmieh Kedokteren)
(Center for Medicel Science Communit!)
: GRAMIK
March' April-June' Jutv-September' october-December)
UniYersity School of Medicine
l;;;;iJ-r*
Address
'
iirt"ogg,
60 I 3 I
Jl. Prof dr Moestopo 47 Surabaya
ext l35
Phone: 62-31-5013749, 5020251-3
62-31-50224'12
49,
t-5013'l
Fax : 62-3
E-mait: foliamedica@ gmail'com
Accredited Bo. 2/E/KPT/201
5
FOLIA MEDICA INDONESIANA
ISSN 2355-8393
Vol. 52 no.
l
January - March 2016
CONTENTS
1-6
Ahmad Yudianto, Agung Sosiawan, Nola Margaret)
'TRCODIS(CSF,IPO,THOI,TPOX,VWA)LOCIGENETICVARIATIONANALYSISINMADURESE
\NALYSISOFINOUCTIONPHASEGLUCOCORTICOIDUSEONADRENALSUPPRESSION
N PEDIATRIC PATIENTS WITH ACUTE LYMPHOBLASTIC LEUKEMIA
Octaviana Simbolon, Yulistiani, IDG Ugrasena, Mariyatul Oibtiyah)
7
ISCHEMIA REPERFUSION
rHE ACUTE EFFECT OF ERYTHROPOIETIN ON GLUCOSE LEVELS DURING
NJURY IN RATS
Apostolos Papalois)
Constantinos Tsompos, Constantinos Panoulis, Konstantinos Toutouzas' George Zografos,
1OLE OF HEPATITIS B ANO HEPATITS C VIRUS COINFECTION IN TB DEVELOPMENT
Pudji Lestari, lvan Rahmatullah, Dwi Susanti)
'.13
t4-18
19-23
]oRRELATIoNBETWEENINTRACELLULARHEATSHocKPRoTEINT0ExPRESSIoN
rru6 cinvrcar- r-vMpH NoDEs ENLARGEMENT lN NASoPHARYNGEAL cARclNoMA
24-34
Achmad C Romdhoni, Sri Herawati, Elida Mustikaningtyas)
OF
:FFECT oF cayratia trifotia L (Domin) EXTRACT oN EEotlgE-P lllREssloN
FACToR
GROwTH
ENDoTHELIAL
vlscuLAR
vrarnixrrrerl-lOpnorrlr'rese-s (Mfue-s1 aHo
,VEGF-A)
CANCER
BREAST
WITH
RATS
IN WHITE
Devi Cynthia Dewi, lKetut Sudiana)
-
-A
35-41
qNALYSISoFDEFERASIRoxANDDEFERIPRoNUSEINcHILoRENWITHPEoIATRIc
].THALASSEMIA MAJOR
'Evy Sari Sutrisnaningsih. Suha4ono. Bambang Sudarmanto)
42-46
EFFECT OF BODY MASS INDEX (BMI) ON MORTALITY AND READMISSION
ci iiani
mruunE PATIENTs lN cAnotovescut-rR ourPATlENr cARE uNlr
AT DR. SOETOMO HOSPITAL, SURABAYA
:Sella Rizkita Lestari, Andrianto' J Nugroho)
47-50
EARLY INTERVENTION OF CHILD
IMPLEMENTATION ON STIMULATION' DETECTION, AND
dio-wrx eNo
oevELoPMENT (sDlDTK) PRocRAM lN PUSKESMAS MoJo'
iunaelva srtuL EMPHASIzE
oN GRowrH ScREENING
(Dwi Susanti, Florentina Sustini)
IN DR. SOETOMO HOSPITAL SURABAYA
SIX YEARS COCHLEAR IMPLANT PROGRAM REPORT
(Haris
N,l
Ekorini)
ON MALE
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT
NECROSIS
WHITE RATS KIDNEY
n"rnonrn9"n Naibaho' Arifa Mustika, Anny Setijo Rahaiu)
5l -56
57-61
52-65
lnrJy
INTRAPROCEDURAL STENT THROMBOSIS IN PERCUTANEOUS
CORONARY ANGIOPLASTY
(Yudi Her Oktaviono)
ENDORECTAL PULL'THROUGH'
SECONDARY LAPAROSCOPIC ASSISSTED TRANSANAL
A PRE.ELIMINARY STUOY
(Barmadisatrio, Hariono)
66-73
74-79
:
Folia Medica Indonesiana Vol. 52 No. I January - March 2016 : 62-65
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE
WHITE RATS KIDNEY NECROSIS
Ardy Hamonangan Naibahor, Ari[a Mustika!, Anny Setijo Rahaju]
rMedical Doctor Program, rDepanment of Medical Pharmacology.
rDepartment ofAnatomic Pathology, Faculty of Medicine,
Airlangga University, Surabaya
ABSTRAK
Daun Singawalang digunakan masyarakat sebagai obat tradisiohal penderita pneumonia akibat penyakit TBC. Upaya penelitian
bidang bitani naipui farmakologi terhadap daun singawalang (Petiveria alliaceae) sudah banyak dilakukan. Beberapa penelitian
bertuiuan mengetaiui khasiat daun tersebut sudah dilakukan, namun penelilian unluk mengelaltui keamanan penggunaannya masih
ini merupakan
iaraig. Peneliian ini be ujuan untuk mengetahui toksisitas jangka pendek ekstrok daun Singalawang. Penelitian
-jenis-ldboratorium
eksperimental dengan rancangan penelitian lhe posl - lest only conlrol group design. Metode pengambilan
'sampel
iolah simple rindom sampling, mengguhakon sanpel 32 ekor mencil (Mus musculus) jailan sebagai hewan coba dengan
berir badan rata-rata 20 gram dan berumur kurang lebih 2 bulan. Hewan percobaan dibagi ke dalam 4 (empa) kelompok, masingmasing kelompok terdiri dari 8 (delapan) ekor mencit yaitu, kelompok kontrol (P0) mendapat aquadest sebagai Pelarut bahan.
Keloipok peitakuan I (Pl) mendapatkan ekstak etanol daun singawalang (Petiveria alliaceae) dosis rendah yaitu 91mg/kgbb.
Kelompok'perlakuan 2 (P2) nendapatkan ekstrak etanol daun singawalang (Petiveria alliaceae) dosis sedang yaitu |80ng/kgbb.
Kelompok-perlokuan 3 (P3) nendapatkan ekstrak etanol daun singawalang (Petiveria allidceae) dosis tinggi yaitu 360mg/kgbb.
Ekst;k et;nol diberikah secara peroral mengguhakan sonde sehari sekali seloma 30 hari. Hasil penelitian ini menunjukkan bahwa
ektrak etanol daun singau,alang (Petiveria alliaceae) ini ti
of
short-term (sub-acute) toxicity tests on
singowalang (Petiveria alliaceae) leaf extract on
necrosis of the kidneys of male white mice (Mas
musculus\ for 30 days showed that the leaf extract of
singawalong (Petiveria alliaceoe) from Batu, East Java,
showed that it did not induce renal necrosis in doses of
90 mg/kgbw, 180 mg/kgbw and 360 mg/kgbw.
DISCUSSION
caused by exposure lo sinSowalang leaf extract. Murine
renal tubular necrosis was found in 8th experimental
animal
Results of sub-acute toxicity test of the leaves
singawalang (Petiveria olliaceae) in the kidney
of male white mice (Mus musculus)
Results
The observations in the control group (P0) receiving
aquadest I ml/day for 30 days did not cause kidney
necrosis. Kidney necrosis on 8th rePlication was not
'7
l.
Short-term toxicity tests on extmct of singa-walang
(Petiveria alliaceae) white to the kidney of male white
6Yo. The mouse was the one that was
mice (Mus musculus) revealed not to caus€ necrosis
found dead on the morning ofthe I I th day, so it was not
the subiect of the experimental animals that was taken
into account. Observations in group I (Pl) singawalang
leaf extract (Petiveria alliaceae) 90 mglkg in mice
(0-269 gok) I ml/day for 30 days did not cause kidney
necrosis on the experimental animals.
of
the kidneys. The results of this study were similar to
those found by Zakaria in 2010, which proYed that the
ethanol extract ofthe leaves do not have acut€ toxicity.
Toxic effect can be reversible and ineversible. Toxic
effect can be reversed when the body is exposed to low
levels or for a short time, while ineversible effects can
be generated from exposure to higher levels or for a
long time (Lu, 1995). Therefore, in elevated level and
extended test time in chronic toxicity test irreversible
toxic efI€cts might be generated, which in this regard
in group 2 (P2) receiving singowalang
leaf extmct (Petiveria alliaceae) 180 mg/kg in mice
(0.528 go/.) I ml/day for 30 days did not cause kidney
necrosis on experimental animals. The observation of
group 3 (P3) Award singawolang leaf extact (Petiveria alliaceae) 360 mg^g in mice (1.056 {/.) | mllday
for 30 days also did not cause kidney necrosis on the
Observations
was necrosis.
experimental animals.
64
Short-Term Toxiciry Test ofSingalawang Extract on Kidney Necrosis (Ardy Hamonangan Naibaho, Arifa Mustika, Anny Selijo
resutt
Functional elfects usually reversible, whereas the
morphological e(fects usually ineversible. Several
studies have been conducted to find functional changes
that can be identified in advance, or can be detected in
animals in lower doses than the dose that causes
of
R haiu)
shon-term toxicity test (sub-acute) of
singawalang (Petiveria alliaceae) leafextract on kidney
necrosis of male white mice (Mus muscalus) for 30 days
showed that singawalang (Petiverid alliaceae) the leaf
extract is not nephrotoxic.
morphological changes. Evidence gathered from liYer
and kidney tests seemed not supporting this notion.
REFERENCES
However, recent discoveries showed that functional test
may be more sensitive. In addition, functional testing is
valuable for the continued monitoring oftoxic effects on
target organs in the long-term studies in animals and
humans (Lu, 1995). The present study does not address
Lien, Y-HH, et aI.2006. Kidney [nternational: SlPlSelective Agonist, SEW287 t, Ameliorates Ischemic
Acute Renal Failure. NPG. USA
needed further research.
Lu, Frank C. 1995. Basic Toxicology: Fundamentals,
Target Organs, and Risk Assesment. Hemisphere
CONCLUSION
Publishing Corporation. New York. Page: 46-65
Sarman. 2008. Singawalang, Si Tangguh Melawan
TBC. Accessed: 25 llly 2012. Cited from: http://
the toxicity
of ethanol extract on organ function
that
omnilogos. blogspot.com,/2008ll2lsingawalang-sitangguh-melawan-tbc.html
7akaia.2010. Uji Toksisitas Akut Daun Singawalang
pada Mencit Putih Jantan Laporan Penelitian.
of
leaves .tlTgalralar?g does not
cause death if exposed once and the reaction is awaited
for 24 hours in doses of 90 mg/kg bw, 180 mg/kg bw,
The ethanol extmct
360 mg/kg bw. This proves that the extract of
Surabaya
singowalang leaves does not have acute toxicity. The
65