Lampiran 1. Data Asli Pengukuran Suhu Rektal Tikus Putih pada Setiap Kelompok Perlakuan yang Berbeda Uji Efek Antipiretik Infusa Batang Brotowali (Tinospora Crispa (L.) Miers) Pada Tikus Putih Jantan Galur Wistar Yang Diinduksi Vaksin Dpt.

58

Lampiran 1. Data Asli Pengukuran Suhu Rektal Tikus Putih pada Setiap
Kelompok Perlakuan yang Berbeda
NO

BB

DOSIS

t (a)

t (0)

t (15)

t (30)

t (45)

t (60)

t (75)

t (90)

t (105)

t (120)

1

133

2.00

37.50

37.90

37.80

38.70

38.80

38.60

38.10

38.70

38.40

38.60

1

112

1.68

37.00

37.60

37.90

38.20

37.90

38.70

38.70

38.50

38.70

38.70

1

108

1.62

36.70

36.50

37.90

37.90

38.00

38.90

39.40

38.90

38.70

38.80

1

124

1.86

35.40

36.40

37.40

38.00

38.10

37.90

37.90

38.60

38.20

37.90

1

113

1.70

36.50

36.50

37.20

37.90

38.00

38.40

37.90

38.70

38.50

38.20

2

137

2.06

33.90

35.40

35.60

36.60

36.30

36.30

35.80

35.60

35.40

35.00

2

159

2.39

34.90

36.10

36.30

36.70

36.70

36.90

37.90

37.70

37.20

36.60

2

127

1.91

35.90

37.00

36.80

36.50

36.30

36.20

36.10

36.00

36.50

36.20

2

124

1.86

37.40

37.70

38.10

37.90

37.80

37.70

37.30

36.90

36.50

36.30

2

168

2.52

36.40

37.10

37.20

37.40

37.00

36.10

35.80

35.60

36.40

36.10

3

134

2.01

37.70

38.20

38.40

38.60

38.80

38.10

38.00

37.50

36.90

35.80

3

156

2.34

35.40

36.40

37.90

38.60

38.40

38.00

38.30

38.30

37.00

36.50

3

126

1.89

35.70

36.40

37.90

37.90

38.60

38.80

39.00

37.60

37.40

36.80

3

132

1.98

35.70

36.90

37.20

37.50

37.60

37.90

38.80

37.30

36.30

36.40

3

127

1.91

35.60

38.10

38.50

37.80

38.00

38.60

38.50

37.70

36.70

36.40

4

147

2.21

35.50

37.40

37.00

36.90

36.60

35.90

35.20

34.90

34.10

34.00

4

143

2.15

36.00

37.00

37.20

37.40

37.60

36.80

36.30

35.80

34.60

34.40

4

159

2.39

37.50

37.90

37.10

37.40

37.20

36.90

36.30

34.60

34.20

34.80

4

171

2.57

36.50

37.30

37.40

36.90

36.80

36.70

36.20

35.70

34.50

34.90

4

130

1.95

37.20

37.60

37.50

37.40

37.70

37.20

36.60

35.60

35.10

34.50

5

137

2.06

35.80

37.00

37.60

38.60

38.30

38.00

37.90

37.00

36.50

36.30

5

159

2.39

36.30

37.30

37.60

38.00

38.30

37.70

37.60

36.60

35.50

35.20

5

127

1.91

36.10

37.20

37.80

38.20

38.30

37.90

37.60

36.40

36.00

35.40

5

124

1.86

35.40

37.50

37.90

38.20

38.60

37.80

37.50

36.90

35.20

35.00

5

168

2.52

35.50

37.90

37.70

38.40

38.70

38.40

38.20

37.50

35.40

34.70

59

Lampiran 2. Data Perhitungan Rata-rata Suhu Rektal Tikus pada Setiap
Kelompok dengan Perhitungan Standar Deviasi

t (a)
1.00
SD
2.00
SD
3.00
SD
4.00
SD
5.00
SD

t (0)

t (15)

t (30)

t (45)

t (60)

t (75)

t (90)

t
(105)

t
(120)

36.62

36.98

37.64

38.14

38.16

38.50

38.40

38.68

38.50

38.44

0.78

0.71

0.32

0.34

0.36

0.38

0.65

0.15

0.21

0.38

35.70

36.66

36.80

37.02

36.82

36.64

36.58

36.36

36.40

36.04

1.35

0.91

0.94

0.61

0.62

0.67

0.96

0.92

0.64

0.61

36.02

37.20

37.98

38.08

38.28

38.28

38.52

37.68

36.86

36.38

0.95

0.89

0.52

0.50

0.48

0.40

0.40

0.38

0.40

0.36

36.54

37.44

37.24

37.20

37.18

36.70

36.12

35.32

34.50

34.52

0.83

0.34

0.21

0.27

0.48

0.48

0.54

0.54

0.39

0.36

35.82

37.38

37.72

38.28

38.44

37.96

37.76

36.88

35.72

35.32

0.38

0.34

0.13

0.23

0.19

0.27

0.29

0.42

0.53

0.61

60

Lampiran 3. Data Persentase Efek Antipiretik t(90) dan t(120)
NO

t (0)

t (90)

% efek antipiretik
t(90)

t (0)

t
(120)

% efek antipiretik
t(120)

1

37.90

38.70

102.11

37.90

38.60

101.85

1

37.60

38.50

102.39

37.60

38.70

102.93

1

36.50

38.90

106.58

36.50

38.80

106.30

1

36.40

38.60

106.04

36.40

37.90

104.12

1

36.50

38.70

106.03

36.50

38.20

104.66

2

35.40

35.60

100.56

35.40

35.00

98.87

2

36.10

37.70

104.43

36.10

36.60

101.39

2

37.00

36.00

97.30

37.00

36.20

97.84

2

37.70

36.90

97.88

37.70

36.30

96.29

2

37.10

35.60

95.96

37.10

36.10

97.30

3

38.20

37.50

98.17

38.20

35.80

93.72

3

36.40

38.30

105.22

36.40

36.50

100.27

3

36.40

37.60

103.30

36.40

36.80

101.10

3

36.90

37.30

101.08

36.90

36.40

98.64

3

38.10

37.70

98.95

38.10

36.40

95.54

4

37.40

34.90

93.32

37.40

34.00

90.91

4

37.00

35.80

96.76

37.00

34.40

92.97

4

37.90

34.60

91.29

37.90

34.80

91.82

4

37.30

35.70

95.71

37.30

34.90

93.57

4

37.60

35.60

94.68

37.60

34.50

91.76

5

37.00

37.00

100.00

37.00

36.30

98.11

5

37.30

36.60

98.12

37.30

35.20

94.37

5

37.20

36.40

97.85

37.20

35.40

95.16

5

37.50

36.90

98.40

37.50

35.00

93.33

5

37.90

37.50

98.94

37.90

34.70

91.56

Mean

104.63

99.23

101.34

94.35

98.66

Mean

103.97

98.34

97.85

92.20

94.51

61

Lampiran 4. Hasil Uji Normalitas Data Suhu Rektal Tikus pada Setiap Waktu

Tests of Normality
Kolmogorov-Smirnova
Statistic

df

Shapiro-Wilk

Sig.

Statistic

df

Sig.

t_a

.130

25

.200*

.958

25

.380

t_0

.104

25

.200*

.961

25

.443

t_15

.131

25

.200*

.922

25

.057

t_30

.154

25

.129

.939

25

.143

t_45

.133

25

.200*

.923

25

.059

t_60

.177

25

.043

.930

25

.088

t_75

.166

25

.074

.947

25

.214

t_90

.105

25

.200*

.951

25

.266

t_105

.100

25

.200*

.952

25

.281

t_120

.132

25

.200*

.927

25

.074

persentase_T90

.127

25

.200*

.969

25

.618

persentase_T120

.110

25

.200*

.952

25

.275

a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.

62

Lampiran 5. Hasil Uji Homogenitas Data Suhu Rektal Tikus pada Setiap Waktu
Test of Homogeneity of Variances
Levene Statistic

df1

df2

Sig.

t_a

1.432

4

20

.260

t_0

4.700

4

20

.008

t_15

3.055

4

20

.041

t_30

3.552

4

20

.024

t_45

1.299

4

20

.304

t_60

1.284

4

20

.310

t_75

3.811

4

20

.018

t_90

5.144

4

20

.005

t_105

.709

4

20

.595

t_120

.449

4

20

.772

persentase_T90

2.318

4

20

.092

persentase_T120

1.708

4

20

.188

63

Lampiran 6. Hasil Uji T Berpasangan untuk Menilai Efek Demam dari Vaksin
DPT
Paired Samples Statistics
Mean
Pair 1

N

Std. Deviation

Std. Error Mean

t_a

36.1400

25

.91606

.18321

t_0

37.1320

25

.69084

.13817

Paired Samples Correlations
N
Pair 1

t_a & t_0

Correlation
25

Sig.

.669

.000

Paired Samples Test
Paired Differences
95% Confidence Interval of
the Difference

Mean

Pair 1 t_a - t_0

Std. Deviation

-.99200

.68491

Std. Error
Mean
.13698

Paired Samples Test

t
Pair 1

t_a - t_0

-7.242

df

Sig. (2-tailed)
24

.000

Lower
-1.27472

Upper
-.70928

64

Lampiran 7. Hasil Uji One Way Anova untuk Persentase Efek Antipiretik t(90)
Oneway
Test of Homogeneity of Variances
persentase_T90
Levene Statistic
2.318

df1

df2
4

Sig.
20

.092

ANOVA
persentase_T90
Sum of Squares

df

Mean Square

Between Groups

284.473

4

71.118

Within Groups

119.973

20

5.999

Total

404.445

24

F
11.856

Sig.
.000

65

Lampiran 8. Hasil Uji Post Hoc LSD untuk Persentase Efek Antipiretik t(90)
Post Hoc Tests
Multiple Comparisons
persentase_T90
LSD
95% Confidence Interval
(I)

(J)

kelomp kelomp Mean Difference
ok

ok

1

2

5.40400*

1.54902

.002

2.1728

8.6352

3

3.28600*

1.54902

.047

.0548

6.5172

4

10.27800*

1.54902

.000

7.0468

13.5092

5

5.96800*

1.54902

.001

2.7368

9.1992

1

-5.40400*

1.54902

.002

-8.6352

-2.1728

3

-2.11800

1.54902

.187

-5.3492

1.1132

4

4.87400*

1.54902

.005

1.6428

8.1052

5

.56400

1.54902

.720

-2.6672

3.7952

1

-3.28600*

1.54902

.047

-6.5172

-.0548

2

2.11800

1.54902

.187

-1.1132

5.3492

4

6.99200*

1.54902

.000

3.7608

10.2232

5

2.68200

1.54902

.099

-.5492

5.9132

1

-10.27800*

1.54902

.000

-13.5092

-7.0468

2

-4.87400*

1.54902

.005

-8.1052

-1.6428

2

3

4

(I-J)

Std. Error

Sig.

Lower Bound

Upper Bound

66

5

3

-6.99200*

1.54902

.000

-10.2232

-3.7608

5

-4.31000*

1.54902

.011

-7.5412

-1.0788

1

-5.96800*

1.54902

.001

-9.1992

-2.7368

2

-.56400

1.54902

.720

-3.7952

2.6672

3

-2.68200

1.54902

.099

-5.9132

.5492

4

4.31000*

1.54902

.011

1.0788

7.5412

*. The mean difference is significant at the 0.05 level.

67

Lampiran 9. Hasil Uji One Way Anova untuk Persentase Efek Antipiretik t(120)
Oneway
Test of Homogeneity of Variances
persentase_T120
Levene Statistic
1.708

df1

df2
4

Sig.
20

.188

ANOVA
persentase_T120
Sum of Squares
Between Groups
Within Groups
Total

df

Mean Square

398.135

4

99.534

94.026

20

4.701

492.160

24

F
21.172

Sig.
.000

68

Lampiran 10. Hasil Uji Post Hoc LSD untuk Persentase Efek Antipiretik t(90)
Post Hoc Tests
Multiple Comparisons
persentase_T120
LSD
95% Confidence Interval
(I)

(J)

kelomp kelomp Mean Difference
ok

ok

1

2

5.63400*

1.37132

.001

2.7735

8.4945

3

6.11800*

1.37132

.000

3.2575

8.9785

4

11.76600*

1.37132

.000

8.9055

14.6265

5

9.46600*

1.37132

.000

6.6055

12.3265

1

-5.63400*

1.37132

.001

-8.4945

-2.7735

3

.48400

1.37132

.728

-2.3765

3.3445

4

6.13200*

1.37132

.000

3.2715

8.9925

5

3.83200*

1.37132

.011

.9715

6.6925

1

-6.11800*

1.37132

.000

-8.9785

-3.2575

2

-.48400

1.37132

.728

-3.3445

2.3765

4

5.64800*

1.37132

.001

2.7875

8.5085

5

3.34800*

1.37132

.024

.4875

6.2085

1

-11.76600*

1.37132

.000

-14.6265

-8.9055

2

-6.13200*

1.37132

.000

-8.9925

-3.2715

2

3

4

(I-J)

Std. Error

Sig.

Lower Bound

Upper Bound

69

5

3

-5.64800*

1.37132

.001

-8.5085

-2.7875

5

-2.30000

1.37132

.109

-5.1605

.5605

1

-9.46600*

1.37132

.000

-12.3265

-6.6055

2

-3.83200*

1.37132

.011

-6.6925

-.9715

3

-3.34800*

1.37132

.024

-6.2085

-.4875

4

2.30000

1.37132

.109

-.5605

5.1605

*. The mean difference is significant at the 0.05 level.

70

Lampiran 11. Foto Dokumentasi Penelitian

Persiapan alat

Penimbangan tikus

Thermometer digital

Pembuatan infusa brotowali

71

Perhitungan dosis

Suhu lemari pendingin vaksin

Proses pengambilan vaksin

Vaksin DPT Pentabio

72

Proses induksi vaksin

Proses penimbangan ibuprofen

Proses penggerusan ibuprofen

Pembuatan sediaan ibuprofen

73

Bahan sediaan perlakuan

Pengukuran suhu rektal

74

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