COMPARISON BETWEEN THE EFFECT OF RAW HONEY FROM MADU PERHUTANI AND GEMFIBROZIL ON BLOOD TRIACYLGLYCEROL LEVEL OF HYPERLIPIDEMIC MODEL WISTAR RATS.
ABSTRACT
Hyperlipidemic condition is a health hazard, associated with
cardiovascular diseases. Acquired hypertriglyceridemia can be largely due to
excessive intake of high fat diet as designed in this study, and accumulation of fat
can cause increasing free radical in the body due to oxidative and lipid
peroxidation in presence of metal in vivo. An Indonesian local raw honey from
Madu Perhutani has tested for its antioxidant content, and in this study the aim is
to prove the antioxidant effect towards lowering blood triacylglycerol (formerly
named triglyceride) with gemfibrozil as comparison for effectiveness.
This laboratory based experiment involved 24 subjects from Wistar breed
rats with four different groups. Group I as control negative, with normal subjects
as baseline standard during the whole experiment, while group II or control
positive were hyperlipidemic induced rats without any treatment given. Group III
was hyperlipidemic induced, and given with raw honey from Madu Perhutani,
and group IV was given with gemfibrozil drug concomitantly induced to be
hyperlipidemic. The blood triacylglycerol measurement was done automatically
by principle of enzymatic glycerol phosphate oxidase (GPO).
The result proved that this local honey, with its minimum antioxidant
dosage based on previous antioxidant test has started to give the effect on
preventing blood triacylglycerol level not to rise too high, yet not enough to act
effectively as gemfibrozil. Based on the fact, raw honey from Madu Perhutani
was concluded to give an effect starting from its minimum dosage of antioxidant
activity, 2.4g/200gBW orally.
Keyword : triacylglycerol, honey, gemfibrozil
IV
ACKNOWLEDGEMENT
Praise be upon the greatest Almighty Allah S.W.T for His blessing and
love, let this humble writer able to finish this minor thesis on “The Effect of Raw
Honey from Madu Perhutani on Blood Triacylglycerol Level of Hyperlipidemic
Model Rats in Comparison to Gemfibrozil”. This minor thesis was submitted in
requirement for bachelor degree at Faculty of Medicine Universitas Padjadjaran
Bandung.
Many people were involved in the process of completing this minor
thesis, by guiding and giving opinions, supported and useful recommendation;
therefore a very great appreciation and thanks to the following people :
1.
Dr. Med. Tri Hanggono Achmad, dr. as Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
2.
Eri Surahman, dr., SpAn-K as former Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
3.
Dr. Endang Sutedja, dr., SpKK-K as Vice Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
4.
Rudy Hartanto, drg., M. Si as chairperson in minor thesis seminar.
5.
Yanti Mulyana, Dra., Apt., MS., DMM as secretary in minor thesis
seminar.
6.
H. Dolvy Girawan, dr., SpPD-KEGH., M. Kes as member in minor thesis
seminar.
7.
Suryosutanto, dr.,as Head of Pharmacology Department.
V
8.
Prof Dr. Rully M. A. Roesli,dr.,SpPD-KGH as Head of Internal Medicine
Department.
9.
Emma Nurdiamah, Dra, Apt. M. Kes., as first supervisor for her
supervision and kindness, giving full effort in guiding, to fulfil this
minor thesis task.
10.
Nanny Natalia M.S., dr., SpPD-KEMD, M. Kes, as second supervisor for
her attention and time, shared thought for this minor thesis.
11.
All lecturers from Community Research Programme of Epidemiology
Department.
12.
All staff of Pharmacology Department of Hasan Sadikin Hospital.
13.
My beloved parent, and siblings for their patience, money and love.
14.
My fellow friends for their supports and encouragement.
15.
To every single soul which writer fails to mentioned one by one, for all
the great guidance.
For that, may our almighty bless each and upon everyone for their
kindness in helping hands, minds and times for this minor thesis. However, as
done by undergraduate person, writer fully aware that this minor thesis still needs
a bunch of improvement in many aspects, and truly writer hope that somehow it
can be useful for practical uses, and as a starting point to proceed further better
research
Jatinangor, 9 Jun 2011
Writer
VI
Hyperlipidemic condition is a health hazard, associated with
cardiovascular diseases. Acquired hypertriglyceridemia can be largely due to
excessive intake of high fat diet as designed in this study, and accumulation of fat
can cause increasing free radical in the body due to oxidative and lipid
peroxidation in presence of metal in vivo. An Indonesian local raw honey from
Madu Perhutani has tested for its antioxidant content, and in this study the aim is
to prove the antioxidant effect towards lowering blood triacylglycerol (formerly
named triglyceride) with gemfibrozil as comparison for effectiveness.
This laboratory based experiment involved 24 subjects from Wistar breed
rats with four different groups. Group I as control negative, with normal subjects
as baseline standard during the whole experiment, while group II or control
positive were hyperlipidemic induced rats without any treatment given. Group III
was hyperlipidemic induced, and given with raw honey from Madu Perhutani,
and group IV was given with gemfibrozil drug concomitantly induced to be
hyperlipidemic. The blood triacylglycerol measurement was done automatically
by principle of enzymatic glycerol phosphate oxidase (GPO).
The result proved that this local honey, with its minimum antioxidant
dosage based on previous antioxidant test has started to give the effect on
preventing blood triacylglycerol level not to rise too high, yet not enough to act
effectively as gemfibrozil. Based on the fact, raw honey from Madu Perhutani
was concluded to give an effect starting from its minimum dosage of antioxidant
activity, 2.4g/200gBW orally.
Keyword : triacylglycerol, honey, gemfibrozil
IV
ACKNOWLEDGEMENT
Praise be upon the greatest Almighty Allah S.W.T for His blessing and
love, let this humble writer able to finish this minor thesis on “The Effect of Raw
Honey from Madu Perhutani on Blood Triacylglycerol Level of Hyperlipidemic
Model Rats in Comparison to Gemfibrozil”. This minor thesis was submitted in
requirement for bachelor degree at Faculty of Medicine Universitas Padjadjaran
Bandung.
Many people were involved in the process of completing this minor
thesis, by guiding and giving opinions, supported and useful recommendation;
therefore a very great appreciation and thanks to the following people :
1.
Dr. Med. Tri Hanggono Achmad, dr. as Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
2.
Eri Surahman, dr., SpAn-K as former Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
3.
Dr. Endang Sutedja, dr., SpKK-K as Vice Dean of Faculty of Medicine
Universitas Padjadjaran Bandung.
4.
Rudy Hartanto, drg., M. Si as chairperson in minor thesis seminar.
5.
Yanti Mulyana, Dra., Apt., MS., DMM as secretary in minor thesis
seminar.
6.
H. Dolvy Girawan, dr., SpPD-KEGH., M. Kes as member in minor thesis
seminar.
7.
Suryosutanto, dr.,as Head of Pharmacology Department.
V
8.
Prof Dr. Rully M. A. Roesli,dr.,SpPD-KGH as Head of Internal Medicine
Department.
9.
Emma Nurdiamah, Dra, Apt. M. Kes., as first supervisor for her
supervision and kindness, giving full effort in guiding, to fulfil this
minor thesis task.
10.
Nanny Natalia M.S., dr., SpPD-KEMD, M. Kes, as second supervisor for
her attention and time, shared thought for this minor thesis.
11.
All lecturers from Community Research Programme of Epidemiology
Department.
12.
All staff of Pharmacology Department of Hasan Sadikin Hospital.
13.
My beloved parent, and siblings for their patience, money and love.
14.
My fellow friends for their supports and encouragement.
15.
To every single soul which writer fails to mentioned one by one, for all
the great guidance.
For that, may our almighty bless each and upon everyone for their
kindness in helping hands, minds and times for this minor thesis. However, as
done by undergraduate person, writer fully aware that this minor thesis still needs
a bunch of improvement in many aspects, and truly writer hope that somehow it
can be useful for practical uses, and as a starting point to proceed further better
research
Jatinangor, 9 Jun 2011
Writer
VI