Re-Vegetation By Using Symbiotic Mocroorganism and Transfer Technology Of The Takino Multi Function Sheet (Marutaku Sheet).

Revegentation by Using Pant-Symbiotic Microorganisms
and Transfer Technology of the Takino Multi-Function
Sheet (Marutaku Sheet)
By
I Gede Putu Wirawan, I G.K. Susrama,
Wayan Adiartayasa, Tasuku Tanaka, and Takuya Marumoto

Present Achievements
•

Identification and development of the mycorrhizal fungi

•

Production of three model Coco Fiber Multi Function Sheet ; First Year Model (2013),
Second Year Model (2014), and Third Year Model (2015)

•

Re-vegetation test sites in Mt. Batur : Site A (2013), Site B (2015), and Site C (2014)

•

Invited Speaker in The 6th Congress and International Biotechnology Conference 1-4
September 2014 in Palembang (IBC 2014)

•

Keynote Speaker on The 5th International Conference on Bioscience and Biotechnology in
Denpasar, 18-19 September 2014 and Invited Speaker The 6th International Conference on
Bioscience and Biotechnology in Denpasar, 19-20 September 2015

•

Lecture on 29-30 September 2014 in Yamaguchi University, Japan and Workshop 24-25
September 2015 at YU Ube City, Japan

•

Development of Bio-fertilizer containing Mycorrhizal Fungi

•

Patent Drafted at Sentra HKI Udayana University

•

Publication in the International Journal on Bioscience and Biotechnology,
Vol. 2 No. 1, 2014 (Published by Asia-Oceania Bioscience and Biotechnology Consortium
(AOBBC)) and Vol. 3, No. 1, 2015 (Accepted)

Identification and development of the
mycorrhizal fungi
• Acaulospora (Glomales : Acaulosporaceae): A. capsicula , A. koskei,
A. foveata, A. laevis, A. denticulata, A. colombiana
• Glomus (Glomales : Glomeaceae): G. ambisporum,
• Gigaspora (Glomales : Gigasporineae),
•
• Scutellospora (Gigasporaceae): S. calospora,

The Isolation and Identification of Mycorrhizal fungi

(From some Plants in Mt. Batur)

Development of Mycorrhizal fungi Spores by Pot Culture

Produce of Bio-Fertilizer containing Mycorrhizal Fungi

Development by using Zeolite, Perlite, Vermiculite,
and sand of Mt. Batur and Corn, Pine, and Ampupu

Priciple of MF-Sheets

Principle : 1.
2.
3.
4.

Prevent surface soil erosion
Prevent surface water flow
Form the media for better mikroclimate
Enrich the life of microbes

Production of two model Coco Fiber Multi Function Sheet;
First Year Model (2013) and Second Year Model (2014)
•

2013 Model :
• Coco Net used: 1 cm coco rope distance between rope 2-3 cm
• Coco mesh used 1 mm thin
• Mesh put under the net
• Seed bag used : 50 x 50 cm

•

2014 Model :
• Coco net used: 0.5 cm rope and distance between rope 1 cm
• Using three type of MF Sheets:
• Net – mesh – net
• Net – mesh
• Net – coco fiber + coco dash
• Seed bag used : 30 x 30 cm

•

2015 Model : - Combination of the two previous model
- Material use: dush and then mesh of coco fiber put on the lava sand
and stone, and then the net with slime (diameter about 0.5 mm) ropes and
1 cm distance between the ropes

Target Aplication

Implementation of Coco Fiber MF Sheet

Implementation 2015

Prevention of Erosion

After two years

Plants Growth (Photographed on 16 August 2015,
(Dry Season – after 7 months)

Plants colonized with mycorrhiza:
Pinus, Eucalyptus/ Ampupu, Jack
Fruit/Nagka, Gamelina/Jati Belanda,
Lamptoro. All were growing well

Culture and Colonization of Ecto-Mycorrhizal Fungi in some seedling
of Pine, Leucaena, Corn, and Eucalyptus
Evaluation of some
microbes grew under
the coco fiber MF sheet
and bare area without
the sheet

Thank you