M01652

in Shizosaccharomyces pombe

D1 Venny Santosa
10.08

Facts about S. pombe
Haploid cell:
12-14 μm at division
3.5μm width-constant
Diploid cell:
24μm length at division
5μm width-constant

Morgan, D.O. 2007. The Cell Cycle

Cell Cycle Phases

Replication fork
S phase
DNA helicase

Initiation of DNA replication
G1 phase

7

2 5
4 6
3

ORC: origin replication complex
MCM: mini chromosome maintenance
CDK: cyclin-dependent kinase
Pol: polymerase

MCM2-7

Pre-replicative
Complex (preRC)

Pol //

Cdt1 +Cdc6

Pre-initiation
Complex (preIC)

S-Cdk

6

Conservation of MCM Binding Protein protein in eukaryotes

•Oryza sativa
•Homo sapiens
•Mus musculus
•Xenopus
•Drosophila
•S.pombe

7

Cohesion function in sister chromatid maintenance
DNA replication

Acetyltransferase
Eco1/Ctf7
Irradiation
UV-B

DNA damage/ replication errors
Homologous recombination
between sister chromatids

cohesin

PCNA
DNA clamp

RFC
Clamp loader
•Ctf18

•Rfc2
•Rfc3
•Rfc4
•Rfc5

PCNA: proliferating cell nuclear antigen
RFC: replication factor C
Ctf18: chromosome transmission fidelity factor8 18

Overview of DNA replication

transcription

a,b

Cell cycle
progression/ DNA
replication genes

Regulation of S phase entry

Prereplicative complex

ORC: origin recognition complex
MCM: mini chromosome maintenance
CDK: cyclin dependent kinase
CDC: cell division control
CDT: chromatin binding and DNA replication factor
9

The DNA damage response

recruitment

ATM: ataxia telangiectasia-mutated
ATR: Rad3-related

activation

10

Recruitment and action of ATM

G1

Destruction of Cdc25a
Accumulation of
inhibitory phosphorylation

11