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Signifikansi Khamir dalam Pangan
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Telp 027 4-889398; O27 4 -882262; F ax: O27 4-889057 ;
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Tajuk Entri Utama: Kustyawati, Marla Erna
Signifikansi Khamir dalam Pangan/Maria Erna Kustyawati
- Edisi Pertama. Cet. Ke-1. - Yogyakarta: Pfantaxia,
xii + 88 h1m.,' 25 cm

2016

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Bibliografi.: B3 - 88
rsBN 2 918-602-6912-41-L
E-ISBN : 91 B- 602-6912-42-g
1. Mikroorganisme - Eungsi

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IIATIAB ISI

KATA PENGANTAR
DAFTAR ISI
DAFTAR GAMBAR
DAFTAR TABET

1.
BAB 2

BAB


BAB 4

vii
ix
x1

PENDAHULUAN

L

TAKSONOMI DAN IDENTIFIKASI

J

2.1,
2.2
2.3
2.4
BAB 3


v

Pendahuluan
Taksonomi
Isolasi dan Enumerasi Khamir
IclentifikasiKhamir

REPRODUKSI DAN PERTUMBUHAN
3.1 Pendahuluan
3.2 Reproduksi Khamir
3.4 Faktor-faktorPertumbuhan

KHAMIR DALAM PANGAN

4.1
4.2
,1.3
4.1

Pendahuluan

Sejarah Penggunaan Kharnir dalam Pangan
Khamir dalam Produk Pangan
Aktivitas Khamir dalam Bahan Pangan

J
4
13

17
21
21.
21.

31

39
39
40
42
52


Signifikansi Khamir dalam Pongan

viii
BAB 5

57
PEMBUSUKAN PANGAN OLEH KHAMIR
57
5.1 Pendahuluan
58
5.2 Pembusukan pada Produk Makanan dan Minuman
5.3 Kontrol Terhadap Pembusukan Makanan oleh Khamir 76
83

DAF'TAR PUSTAKA

-oo0oo-

IIATIAR GIMBAR


Garnbar 2.1
Carnbar 2.2
Gambar
Gambar
Gambar
Cambar

2.3
2.4
3.1

3.2

Gambar 3.3
Gambar 3.4

Diagram Sebuah Sel Khamir (*Mengandung
Membran Lemak)
Perbedaan Struktur Sel Khamir dan Sel Bakteri

Berbagai Bentuk Askuspora
Morfologi Sel Beberapa Spesies Khamir
Beberapa Sistem Reprocluksi Aseksual Khamir
Proses Pertunasan atau Btdding
Reprocluksi Aseksual dengan Perfunasan
Pembelahan Sel Secara Fisi pacla Siklus Hiclup
S

Gambar 3.5

cl

izo

snccl tfir

o

5
5

6
6
8
22
23
24
25

tilyce s p on rbe.

Cambar 3.6

Pembentukan Tunas dan Pembentukan Spora (Sporulasi) 26
p ada S nccl tfir ofi ty ce s cerett i sise
Siklus Reproduksi Sexual pada Khamir
28

\.ralll0af 5./

lc

Pertumbuhan Sel Khamir
Snccl m ro

Cambar 4.1

t

n

yce s ce rea i si

S. cereaisiae Selama

Waktu Tertentu
Metabolism Lemak, Karbohidrat dan Protein
Melalui Siklus Krebs Sebagai Penghasil Energi

28

53

Signifikonsi Khomir dalom Pangan

Gambar

5.1

Reaksi Dekarboksilasi Pembentukan Senyawa

Kadaverin dan Putresin oleh Mikroflora Pembusuk
dalam Daging

IIATIAR IABTI.

Tabe11.1

Tabel2.2
Tabel3.1
Tal.el3.2
Table 3.3

Tabel

.-1

Tabel3.5
Tal--el3.-1

tr^r

a

I

Kunci Karakteristik unluk Iclentifikasi dan Klasifikasi
Khamir
Medium Wiskerham clan Medium Sporulasi
Temperatur Pertumbuhan Minimum, Optimum,
dan Maksimum Khamir dalam Makanan dan Minuman
pH Minimum, Optimum, Maksimum Pertumbuhan
Khamir clalam Makanan clan Minuman
Nilai a* Minimum Pertumbuhan Khamir dalam
Makanan clan Minuman
Konsentrasi NaCl dan Sukrosa Maksimum unLuk
Pertumbuhan Khamir clalam Makanan clan Minuman
Produksi Berbagai Alkohol oleh Khamir
Konsentrasi Minimum Asam Bensoat dan Asam Sorbat
vang Diperlukan untuk Menghambat Pertumbuhan
Kharnir dalam Makanan dan rninuman pada pH 5,0-5,5.
Khamir vang Terdapat dalam Produk Susu
i.hamrr vang Terdapat dalam Produk Daging
Enz in-L Ekstraseluler Hidrolitik Kharnir
Khamir Pembusuk Produk Daging

15
15

77
32
34
35

36

36

3/

45
51
53
60

Signifikansi Khamir dalam Pangan

xn

Tabe15.2 Khamir Pembusuk Produk
Tabel

5.3

Susu

63

Konsentrasi Minimum Bahan Pengawet yang Diperlukan 79
untuk Menghambat Pertumbuhan Khamir Pembusuk

-oo0oo-

DATTIR PU$ilIM

Barners, E.M., Impey,C.S., Geesory J.D., and Buhagiar, R.W.H., 1978. The
effect of storage temperature on the shelf life of eviscerated air-chilled
turkeys. Br.,Poult. Sci., (191):77 .

Barnett, ]., R.W. Payne dan D. Yarrow. 2000. Yeast: Characteristics and
Identification, 3rd edition, Cambridge University Press, Cambridge.
Bemrion, Marion., 1980. The Science of Food, Harper and Row, Publisher
San Francisco, pp311-312.

Beuchat, L.R. 1993. Selective media for deiecting and enumeration
foodborne yeast. Int. J.Food Microbiol. 797-4.
Biotechnol

Brown,

o

gy . 1.2:1-44.

A. D.,

1990. Compatible solutes and extreme water stress in
eukaryotic microorganisms, Adv. Microbiol. Physiol., 17 :1,81-190
"

Buck, ].D., Bubucis, P.M/. and Combs, T.J., 1977. Occ,arrence of humanassociated yeast in bivalve shellfish from Long Island Sound. Appl.
Environ.Microbiol. (33):37 0.

Carlin, F., Nguyen-The,C., Chambury,Y., and Reich, M. 1990. Effects of
control atmospheres on microbial spoilage, electrolyte leakage and

Signifikonsi Khamir dolom Pongon

B4

sugar content of fresh ready to use grated carrots. Int. J. Food
technol (25):110.

Cole, M.B., and Keenan, M.H.J., 1986. Synergistic effects of weak-acid
preservatives and pH on the growth of Zygosncchnrontyces bnilii. Yeast
(2):e3.

Dalton, H.K., Board,R.G., and Davenport,R.R., 1984. The yeasts of British
fresh sausage and minced beef, ]. Microbiol. Serol', Anthonie van
Leeuwenhook (50):227.
De Boek, E., and Kuik, D.,1987. A survey of the rnicrobiological quality of
the blue-veined cheese. Neth. Milk Dairy technol $1):227.
Deak, T.1997. Foodborne yeast. Ads Appl Microbiol36:179-27I
Deak,T. 1995. Methods for the Rapid Detection and identification of yeasts
in foods. Trends in Food Science and Technology 6:287-292.
Fell, J.W., Boekhout, T., Fonseca, A., Scorzetti,G., Statzell-Talman, A. 2000.

Biodeversity

and systimatics of

basidiomycetous yeasts

as

cletermined by large subunit D1,/D2 domain sequence analysis. Inter.
Journal of Sistymatic and Evolutionary Microbiology. 50:1351-1371'

Flaningary 8., 1987. The rnicroflora of barley and malt in Brewing
Microbiology, priest, F.G., and Campbelt., I., Eds., Elsevier., London.,
83.

Fleet G.H.,L999. Microorganisms in Food Ecosystems

inInt.l.

Food Mictobiol.

50,101.-1.07.

Fleet, G.H. 1990. Yeasts

in dairy products-areview. I.Appl Bacteriol. 68:199-

211.
Fleet, G.H.1992. Spoilage yeasts. Crit Rev Biotechnol 12:7-44.

Fleet, G.H. 2006. The Yeast Handbook. Amparo Querol, Graham H. Fleet
(Eds): Yeasts in Food and Beverages. Springer-Verlag Berlin

Heidelberg. Chapter

1.

yeasts as primary
a*
contaminats" ^^.
in yogurt
"r""":"Y;?:^,1ll
l,r?ur_
"6.
produced commercialry
in
-/

Logos. Nigeria. J. Food protech. g50):1g3.

J' ancl Bruner I.R. 1982. Koumiss procluced from
skim milk sweet
[cuan whey blend.
Cultured Dairy Iournal, 22(7):23.
Hsieh, D.Y., and Juy,
J.M. 1984. Characterization and identification
of yeasts
from fresh and spo,ed ground
beef. Int.J. Foocl Micro biol.,93):147.
lu:" I'' Loessner' M'J', Gorden, D.A. 2005. Modern
Food Microbiorogy,
seventh ed., Springer, IJSA, pp
101_118.

la'v' J'' Loessner' M'J', Golclen, D.A. 2005.
Modern Food Microbiology,
seventh ed., Springer, USA, pp
415_433.

86

Signifikansi Khamir dalom pangan

Jermini' M'F'G'' Geigers,o', and
schimdt-Lorenz,w -M.,
7987. Detection
isolation and identification
of osmotoierant yeasts from
high_sugar

products. J.Food protect.
(S0):a68.

Johnson, D.A., Regner, K.M., and
Lunclen,I.D. 1989. yeast
soft rot of onion
in the WaIIa valley of Washington. plant
Dis. (73):6g6.

Kallmeyer, Moritz., 2005. yeast
Autolysis. Chief Brewer Drayman's
Microbrewery,
pretoria,
Silverton

March 2005.

Kurtzman, C'p. ancl
J.w. Felr. 1ggg. The yeast.
editiory Elsevier, Amsterdam.

A

Taxonomic Study,

4tn

Kurtzmary C'p'' and Robnett,
cJ.,lgg'.Iclentification ancl
phyrogeny of
ascomycetous yeasts from
anarysis of ,u.g" ,rbu nit
(26s) ribosomal
DNA partial sequences.
Antonie Van Leeuirenhoek
7B:ZZ7_277.

Lenoir, 1.1984. The surface
flora and its role in the ripening
of cheese. Int.
Dairy Fed. BuIl. (17t):3.
Lodder

I'

J.

7970. The yenst:

Lowry'p'D',

ancl

A Tnxononti sttdy,North Holrand pubrishing
Co.
Gin,c.o., 1984. Development

lamb stored at _5oC. Food protect.
J.

of yeast microfrora on frozen
$7):309.

Mambetaliev B.D', 1990. production
of Koumiss. ussR patent.

s,

1: 544,

Mian, M'A., Fleet, G.H., Hocking,
A.D., 7gg7. Effect of diluent
type on
viability of yeasts enumerated
from foocrs or pure curfure.
Inten.
Journal of Foocl Microbilogy . 88:703_107.
onishi' H' 1ggo' yeasts in
fermented

foods. In, spencer,
J.F.T., and spencer,
D.M., (Eds.), yenst Technology.springer_V
erlag,Berlin. p 767.
Parish' M'E ancl Higgins,
D.p.,lggo.Investigation of
the microbial ecorogy
of commercial grapefruit
sections. J.Food protech.,
(53):6g5.
Pitt,I.L, and Hockir
sp oila ge. s econd e di
riory
Br ackie A.u d ux:

J;lijl;*xll;:

#.",

Daftor Pustoka

t'itt, ].I., ancl Hotchking, A.D. 198g. problem with
preservative resistant
yeasts. Microbiol. Aliment. Nutr. (6):19.

w. 1996. Growth, metabolic ancl ultrastructurar properties of
foocl spoilage yeasts culfuretl under clifferent environmental

Praphailong,

conditions. PhD Thesis, Department of Food science
and rechnology,
The University of New South wales, Syclney NSW,
Australia.
Praphailong,

w. and G.H. Fleet.

1997. The effect of pH, sodium chloride,
sucrose, sorbate and benzoate on the growth of
spoilage yeast. Foocl

Microbiol.,

74:459 -468.

Publishing Co.
Reed,G., and Nagodawithana, T.w., 1991.
Technology
winemaking., Am.J.Enol.Vitic., 39:83.

of yeast usage in

Roostita R. 1993. occurrence, Growth and Biochemical properties
of yeasts
in Cheeses and Milk. A Thesis, The university of New
South wa1es,
Australia.
Roostita R. and Fieet G.H. 1996. Growth of yeasts
in Milk ancl Associated

Changes to

Mi cr ob i ol o gy

Rose,

31

milk Composition. lntenmtiortnl lorrnnl ,f

Food

: 205 -279 .

A'H', and Harrison, r.s., 7ggz. The yeasts, second edition,
Yeast Technology, Academic press, Loncion.

vor.

5,

Schmid, A., Dordick , J.5., Hauer, B., Kiener,
A..,Wubbo1ts, M. & Witholt, B.
2001. Indushial biocatarysis today and tomorrow.

Nature 409:

268.

258_

Siward, R.F., Lee, B.H., Laley, C.L. and
Ho1ley, F.I. 1983. Effecs of
temperafure, light and storage time on the
microflora of vacuum

or
nitrogen-packed frankfurters. Food protect.
J.
$6):199.
Spencer, I.F.T., and Spencer, D.M., 1990. yeast
Technology., Springer
Verlag, Berlin Heidelberg.

surajudin, Fauzi R. Kusuma, Dwi purnomo.,
2005. yogh rrt: sttstt Fermentnsi
y an g

Meny ehatkan, Jakarta : A groMe dia pustaka.

88

Signifikansi Khamir dalam Pongan

Tudor, E.A., Board,R.G., 1993. Foods Spoilage Yeasts, In: Rose, A.H..
Horrisory J.S., (Eds), The Yeast second edition volume 5. Yeast
Technology. Academic Press, London. Pp 435-516.

Walker, G.M.,1998. Yeasts physiology and biotechnology. John Wiley ancl
Sons, Chichester.

Walker, H.W. and Ayres, J.C.1,970. Yeasts as spoilage organisms. In Rose,
A.H., and Harrison,J.S., (Eds.). T'|rc Yenst Vol 3, Yenst Technology,
Acaclemic Press., London. P 463.

Wart, A.D. 1989. Transport of benzoic ancl propanoic acicls brZy go s acchnroilty

ce

s

b

nilii.

J

. Gen.

Microbiol

(135) :1383.

Wirahadikusumah, 1985. Biokirnia Pangan, institute Pertanian Bogor.

Woolford, M.K., 1985. The silage fermentation, in Microbiology of
fermented foods. Yol2, Wood, B.J.B., eds., Elsevier., Lonclon p 85.

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