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Comparison between Two-Layer Density Gradient and Three-Layer Density Gradient Technique for Sperm Preparation at Aster Fertility Clinic, Dr. Hasan Sadikin General Hospital | Astarto | International Journal of Integrated Health Sciences 286 956 1 PB

Original Aricle
Comparison between Two-Layer Density Gradient and Three-Layer Density
Gradient Technique for Sperm Preparaion at Aster Ferility Clinic, Dr. Hasan
Sadikin General Hospital
Nanang Winarto Astarto,1 Dian Tjahyadi,1 Sintya Jatnikasari2
Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin
General Hospital
2
Aster Ferility Clinic, Dr. Hasan Sadikin General Hospital
1

Abstract

Objecive: To compare the eicacy of sperm preparaion techniques modiied by
two layer density gradient technique (80%, 40%).
Methods: Sperm preparaion was performed by two- and three-layer density
gradient technique by centrifugaion at 500 rpm for 20 minutes using SpermGrad
(Vitrolife, Sweden).
Results: The average concentraion of total sperm preparaion by two-layer
density gradient was 23.19 million with a standard deviaion of 11.9 million while
the result of three-layer density gradient preparaion obtained a mean total

sperm concentraion of 19.64 million with a standard deviaion of 10.033 million.
The mean total concentraion of sperm in three-layer was lower than two-layer
density gradient with p

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