The Effect of Decis Insecticide on the Mortality and Gill Histological Structure of the Red Indigo "Cangkringan local strain" | Wulandari | Jurnal Sain Veteriner 3814 7436 1 PB

JURNAL

JSV 31 (2), Desember 2013

SAIN VETERINER
ISSN : 0126 - 0421

Efek Insektisida Decis terhadap Mortalitas dan Struktur Histologis Insang Ikan
Nila Merah “Lokal Cangkringan”
The Effect of Decis Insecticide on the Mortality and Gill Histological Structure of the Red
Indigo “Cangkringan local strain”
Wahyuni Wulandari1, Sukiya1 dan Suhandoyo1
1

Universitas Negeri Yogyakarta, Yogyakarta
Email: niwul.bio@gmail.com
Abstract

This study was aimed to determine the effects of Decis insecticide on the mortality, its safe level
concentration against mortality, and the cytotoxic effects in the gills of the red tilapia strains of "local
Cangkringan" collected from Fish Seed Center, Cangkringan, Sleman, Yogyakarta. Eight hundreds fish

weighing 0.5-1.0 g and 2-12 weeks old each were collected randomly and divided into 5 groups with Decis
insecticide concentration of 0.27 ppm, 0.81 ppm, 1.00 ppm, 1.35 ppm, 2.4 ppm, respectively and a control group
based on preliminary testing. Each treatment comprised three replicates, 10 replicates of each test fish. The
observations of fish mortality to the test were done every hour - 24, for 96 hours of treatment, whereas mortality
at safe levels test done every 4 weeks for 2 months of treatment. Data were analyzed using probit analysis to
determine LC50 and LC50-96 hours-48 hours. The assay of 10% safe levels of LC50-48 hours is 0.13 ppm. Twofactorial analysis of variance was to determine the effect of concentration and duration of exposure to the Decis
insecticide test fish mortality. Independent samples of T test analysis was applied to determine the effect of safe
levels. The descriptive and qualitative analysis was done to determine the pathologic lesions on fish gills due to
the cytotoxicity of Decis insecticide. Results of the present study showed that the variation of concentrations of
Decis insecticide has significant effect on fish mortality (p

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