Alem Pramudita Wibowo 22010113120094 Lap.KTI Bab7

DAFTAR PUSTAKA

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13.


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53

Lampiran 1. Ethical Clearance


54

Lampiran 2. Izin penelitian

55

Lampiran 2. Izin penelitian (lanjutan)

56

Lampiran 2. Izin penelitian (lanjutan)

57

Lampiran 3. Informed consent

58

Lampiran 3. Informed consent (lanjutan)


59

Lampiran 4. Prosedur pembuatan preparat
: Korelasi Luas Area Wharton’s jelly dengan Luaran Berat Lahir

Judul

Bayi pada Kehamilan Cukup Bulan
Tempat
1.

: Laboratorium Sentral RSUP Dr. Kariadi

Potong tali pusat sepanjang 2 cm dari ujung akhir
pemotongan, lalu masukkan kedalam container
yang telah berisi Neutral Buffer Formalin dengan
perbandingan 1:10. Beri Identitas dan bawa ke
laboratorium Patologi Anatomi

2.

Jaringan mentah dipotong menjadi 3 bagian (pilih
bentuk potongan yang baik) lalu masukkan
kedalam kaset. Lalu, masukkan kaset kedalam
Neutral Buffer Formalin.

3.

Pemasakan jaringan selama 17,5 jam :
Kaset dimasukkan kedalam keranjang, kemudian
masukkan kedalam alat pemasakan jaringan
Microm STP 120, yang terdiri atas beberapa
tabung dengan lama masing-masing waktu
perendaman :
-

Formalin 10% 1 selama 2 jam

-

Formalin 10% 2 selama 2 jam

-

Alkohol 70% selama 1 jam

-

Alkohol 80% selama 1 jam

-

Alkohol 96% selama 2 jam

-

Etanol selama 2 jam

-

Xylol selama 1 jam

-

Parafin 1 selama 2 jam

-

Parafin 2 selama 2 jam

-

Parafin 3 selama 2 jam

60

4.

Jaringan yang telah melalui proses pemasakan
kemudian

dibuat

menjadi

blok

jaringan

menggunakan alat histocenter . Kemudian blok
parafin didinginkan kedalam freezer.

5.

Setelah didinginkan, blok jaringan kemudian
dpotong

menggunakan

mikrotom.

Hasil

pemotongan dimasukkan kedalam air hangat lalu
ditempelkan pada kaca objek. Beri identitas

6.

Preparat kemudian dipanaskan dalam oven 70%
selama 10 menit untuk melelehkan sisa parafin.
Kemudian dinginkan dan tata di rak.

7.

Proses pewarnaan dengan alat Veristein dengan
total waktu 2,5 jam :
-

Xylol 1, 2, dan 3 masing-masing 10 menit

-

Etanol selama 3 menit

-

Alkohol 96% selama 3 menit

-

Alkohol 80% selama 3 menit

-

Alkohol 70% selama 3 menit

-

Alkohol 50% selama 3 menit

-

Bilas dengan air mengalir

-

Hematoksilin selama 15 menit

-

HCl selama 1 menit

-

Bluing selama 10 menit

-

Air mengalir

61

-

Alkohol 50% selama 3 menit

-

Alkohol 70% selama 3 menit

-

Eosin selama 3 menit

-

Alkohol 70% selama 3 menit

-

Alkohol 96% selama 3 menit

-

Etanol selama 3 menit

-

Xylol 1, 2, dan 3 masing-masing selama 10
menit

8.

Preparat

yang

telah

diwarnai

kemudian

dikeringkan. Setelah itu di lem menggunakan EZ
Mount dan tutup menggunakan deck glass.

62

Lampiran 5. Lembar pengumpulan data
No sampel

:

A. IDENTITAS
Nama

:

Tempat/ tgl lahir :
Alamat

:

No. HP

:

No CM/ Regis

:

B. RIWAYAT KEHAMILAN
ANAMNESIS
1. Usia ibu

: …… tahun

2. Paritas

: G…. P…. A….

3. Pendidikan terakhir

: a. Tidak sekolah
b. SD/sederajat

d. SMA/sederajat
e. Perguruan Tinggi

c. SMP/sederajat
REKAM MEDIS
1. Interval kehamilan

: …… bulan

2. Usia Kehamilan

: …… minggu

3. Tinggi badan ibu

: …… cm

4. GDS

: …… mg/dl

5. Hb

: …… mg%

6. Ureum

: …… mg/dl

7. Kreatinin

: …… mg/dl

8. HbsAg

: a. Positif

b. Negatif

9. Riwayat gangguan fungsi jantung selama kehamilan?
a. Ya

b. Tidak

10. Riwayat terdiagnosis komplikasi medis atas kehamilan?
a. ya, sebutkan ………………………

b. Tidak

63

C. DATA BAYI
Jenis kelamin

:

Berat lahir

:

D. HASIL PEMERIKSAAN HISTOFOTOMETRI
L.TP

L.A1

L.A2

L.AT

L.V

mm2

Keterangan
L.TP

: Luas area tali pusat

L.A1

: Luas area arteri umbilikal 1

L.A2

: Luas area arteri umbilikal 2

L.AT

: Luas arteri umbilikal total (L.A1 + L.A2)

L.V

: Luas area vena umbilikal

L. PD

: Luas area pembuluh darah (L.AT + L.V)

L.WJ

: Luas area Wharton’s jelly (L.TP – L.PD)

Semarang,
Yang mengisi,

(………………………………)

2016

L.PD

L.WJ

64

Lampiran 6. Hasil Pemeriksaan

1

Usia
Ibu
29

2

33

2

Perguruan Tinggi

21

40

156

11.1

Perempuan

3600

32.683

1.668

1.418

3.086

3.090

3

25

2

SD/sederajat

29

37

156

11.6

Perempuan

2900

53.829

1.845

1.690

3.535

2.977

6.512

47.317

4

29

0

SMA/sederajat

0

39

158

10.5

Laki-laki

3950

84.144

2.218

5.278

7.496

4.474

11.970

72.174

5

29

1

Perguruan Tinggi

24

38

165

11.3

Perempuan

2700

59.081

2.936

2.222

5.158

3.323

8.481

50.600

6

27

1

SMA/sederajat

62

39

158

10.6

Laki-laki

3750

49.688

2.497

2.141

4.638

3.559

8.197

41.491

7

35

1

Perguruan Tinggi

76

39

153

9.8

Laki-laki

3600

79.064

2.759

4.160

6.919

5.117

12.036

67.028

8

26

0

Perguruan Tinggi

0

40

170

12.9

Perempuan

2950

80.114

3.857

2.734

6.591

4.809

11.400

68.714

9

27

1

Perguruan Tinggi

24

39

155

11.5

Perempuan

3100

62.376

2.444

3.283

5.727

3.481

9.208

53.168

10

23

0

Perguruan Tinggi

0

40

155

10.8

Perempuan

2950

58.791

3.242

1.718

4.960

2.964

7.924

50.867

12

25

0

SMA/sederajat

0

40

156

12.2

Perempuan

2600

60.362

1.977

2.841

4.818

5.744

10.562

49.800

13

23

0

Perguruan Tinggi

0

40

159

10.6

Perempuan

3000

44.148

1.544

1.527

3.071

3.108

6.179

37.969

14

27

1

Perguruan Tinggi

20

40

164

11.1

Perempuan

3600

53.961

3.236

3.190

6.426

4.312

10.738

43.223

16

32

0

Perguruan Tinggi

0

38

148

11.3

Laki-laki

2750

82.841

2.008

3.299

5.307

6.361

11.668

71.173

17

29

0

Perguruan Tinggi

0

40

164

11.2

Laki-laki

3550

95.206

3.800

4.515

8.315

6.596

14.911

80.295

19

34

1

Perguruan Tinggi

84

37

155

11.2

Laki-laki

3000

89.996

2.148

2.551

4.699

4.150

8.849

81.147

20

29

0

Perguruan Tinggi

0

37

157

11.3

Perempuan

2800

56.036

2.167

2.545

4.712

4.731

9.443

46.593

21

36

1

Perguruan Tinggi

24

37

165

10.5

Laki-laki

4000

122.881

3.453

10.133

13.586

3.391

16.977

105.904

22

27

1

SMA/sederajat

24

37

150

10.7

Perempuan

2500

43.546

2.340

2.271

4.611

3.314

7.925

35.621

23

23

0

SMP/sederajat

0

38

159

11.7

Laki-laki

2500

32.317

1.458

2.297

3.755

2.216

5.971

26.346

25

29

1

Perguruan Tinggi

45

39

148

Perempuan

2900

50.018

2.401

1.983

4.384

2.866

7.250

42.768

26

26

0

Perguruan Tinggi

0

39

153

Laki-laki

3000

46.191

1.995

2.128

4.123

2.427

6.550

39.641

27

24

0

Perguruan Tinggi

0

38

155

Laki-laki

3000

57.387

2.655

3.055

5.710

4.043

9.753

47.634

No

Perguruan Tinggi

Interval
Kehamilan
0

Usia
Kehamilan
39

Tinggi
Badan
155

Paritas

Pendidikan

0

Hb

Jenis Kelamin
Bayi

11

Laki-laki

Berat
Lahir Bayi
2900

L.TP

L.A1

L.A2

L.AT

L.V

L.PD

L.WJ

71.063

2.528

2.367

4.895

3.014

7.909

63.154

6.176

26.507

65

Lampiran 6. Hasil Pemeriksaan (lanjutan)

28

Usia
Ibu
31

29

34

1

Perguruan Tinggi

30

36

2

SMA/sederajat

84

38

160

10.1

Laki-laki

3400

84.253

2.673

1.914

4.587

6.086

10.673

73.580

31

38

2

Perguruan Tinggi

108

39

160

12.2

Laki-laki

2900

59.083

3.954

3.058

7.012

5.165

12.177

46.906

34

32

2

SMA/sederajat

48

39

156

9.9

Laki-laki

2900

68.245

1.677

1.879

3.556

2.022

5.578

62.667

35

23

0

SMA/sederajat

0

39

165

13.1

Laki-laki

3500

112.410

4.413

2.172

6.585

6.619

13.204

99.206

No

Perguruan Tinggi

Interval
Kehamilan
24

Usia
Kehamilan
39

Tinggi
Badan
155

24

38

158

Paritas

Pendidikan

1

L.TP

L.A1

L.A2

L.AT

L.V

L.PD

L.WJ

Laki-laki

Berat
Lahir Bayi
3500

63.064

2.709

2.255

4.964

5.520

10.484

52.580

10.4

Laki-laki

3900

49.217

1.438

2.497

3.935

3.120

7.055

42.162

Hb

Jenis Kelamin
Bayi

66

Lampiran 7. Hasil Analisis
Tests of Normality
Kolmogorov-Smirnova
Statistic
L.WJ

df

.180

Shapiro-Wilk

Sig.
29

Statistic

.017

df

.935

Sig.
29

.073

Tests of Normality
Kolmogorov-Smirnova
Statistic
Berat Lahir Bayi

df

.229

Shapiro-Wilk

Sig.
29

Statistic

.000

df

Sig.

.919

29

.029

Tests of Normality
Kolmogorov-Smirnova
Statistic
Tran_BeratLahirBayi

df

.210

Shapiro-Wilk

Sig.
29

Statistic

.002

df

.931

29

Correlations
Tran_BeratLahir
Bayi
Tran_BeratLahirBayi

Pearson Correlation

L.WJ
1

Sig. (2-tailed)
N
L.WJ

.383*
.041

29

29

Pearson Correlation

.383*

1

Sig. (2-tailed)

.041

N
*. Correlation is significant at the 0.05 level (2-tailed).

29

Sig.

29

.059

67

Descriptives
Statistic
Interval Kehamilan

Mean
95% Confidence Interval for

Std. Error

26.08
Lower Bound

12.69

Upper Bound

39.47

6.488

Mean

5% Trimmed Mean

23.24

Median

21.00

Variance

1052.410

Std. Deviation

32.441

Minimum

0

Maximum

108

Range

108

Interquartile Range

39

Skewness
Kurtosis
Tinggi Badan

Mean
95% Confidence Interval for

1.226

.464

.487

.902

158.28

1.011

Lower Bound

156.19

Upper Bound

160.37

Mean

5% Trimmed Mean

158.23

Median

158.00

Variance

25.543

Std. Deviation

5.054

Minimum

148

Maximum

170

Range

22

68

Interquartile Range

Hb

7

Skewness

.320

.464

Kurtosis

.224

.902

11.144

.1664

Mean
95% Confidence Interval for

Lower Bound

10.800

Upper Bound

11.488

Mean

5% Trimmed Mean

11.111

Median

11.100

Variance

.693

Std. Deviation

.8322

Minimum

9.8

Maximum

13.1

Range

3.3

Interquartile Range

1.0

Skewness

.683

.464

Kurtosis

.436

.902

Descriptives
Statistic
Usia Ibu

Mean
95% Confidence Interval for

29.00
Lower Bound

27.33

Upper Bound

30.67

Mean

5% Trimmed Mean

28.87

Median

29.00

Variance

19.214

Std. Error
.814

69

Std. Deviation

4.383

Minimum

23

Maximum

38

Range

15

Interquartile Range

7

Skewness
Kurtosis

.366

.434

-.846

.845

Jenis Kelamin Bayi
Cumulative
Frequency
Valid

Percent

Valid Percent

Percent

Laki-laki

17

58.6

58.6

58.6

Perempuan

12

41.4

41.4

100.0

Total

29

100.0

100.0

Usia Kehamilan
Cumulative
Frequency
Valid

Percent

Valid Percent

Percent

37

5

17.2

17.2

17.2

38

6

20.7

20.7

37.9

39

11

37.9

37.9

75.9

40

7

24.1

24.1

100.0

29

100.0

100.0

Total

70

Pendidikan
Cumulative
Frequency
Valid

Perguruan Tinggi

Percent

Valid Percent

Percent

20

69.0

69.0

69.0

SD/sederajat

1

3.4

3.4

72.4

SMA/sederajat

7

24.1

24.1

96.6

SMP/sederajat

1

3.4

3.4

100.0

29

100.0

100.0

Total

Paritas
Cumulative
Frequency
Valid

Percent

Valid Percent

Percent

0

13

44.8

44.8

44.8

1

11

37.9

37.9

82.8

2

5

17.2

17.2

100.0

29

100.0

100.0

Total

71

Lampiran 8. Dokumentasi

72

Lampiran 9. Biodata mahasiswa

Identitas
Nama

: Alem Pramudita Wibowo

NIM

: 22010113120094

TTL

: Wonosobo, 28 Agustus 1995

Jenis Kelamin

: Laki-laki

Alamat

: Tawangsari Indah K-12 Wonosobo, Jawa Tengah

Nomor HP

: 082313131919

e-mail

: alempramudita@gmail.com

Riwayat Pendidikan Formal
1. TK

: TK Pertiwi

lulus tahun : 2001

2. SD

: SDN 5 Wonosobo

lulus tahun : 2007

3. SMP

: SMPN 2 Wonosobo

lulus tahun : 2010

4. SMA

: SMAN 1 Wonosobo

lulus tahun : 2013

5. FK UNDIP

masuk tahun : 2013

Keanggotaan Organisasi
1. Staff Informasi dan Komunikasi HIMA KU Undip 2014/2015
2. Staff Membership and Development AMSA Undip 2014/2015
3. Kepala Bidang Informasi dan Komunikasi HIMA KU Undip 2015/2016
4. Executive Board of Asian Medical Students’ Exchange Program AMSA
Undip 2015/2016

Pengalaman Penelitian
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