Effect of Broccoli Flower Extract (Brassica oleracea L. var.italica Plenck) on Inhibition of Photoaging Viewed from Matrix Metalloproteinase-1 Expression in Human Skin Fibroblast
Vol 4, No 26 (2014)
Table of Contents
Articles
Histological observation of Gelam (Melaleuca cajuputi Powell) in different ecosystems of
Terengganu
M. Masitah, A.R. Shamsul Bahri, Jamilah M.S., Salwani Ismail
PDF
Challenges of Utilizing Agricultural Information Sources by Small Scale Farmers in
Nasarawa State, Nigeria
Bello M, C. P. O. Obinne, S. A. Bako
PDF
Circumcision Risks, Benefits and the Prospective
Sokol Buba
1-7
8-16
PDF
17-19
Comparative Antibiotic Activities of Ethanol Extracts of Leaves and Inflorescences of
Mitracarpus villosus from two different Geographical Regions in Nigeria in Synergy and
Antagonism with Conventional Antibiotics
Odima, C. A., Ubani, C. S., Eze, E. A., Oje, O. E., Agu, E. C., Joshua, P. E.
Comparative Study of Training Needs of Forestry Workers in Oyo and Osun States,
Nigeria
Ayandiji, A., Adekunle O.A.
Contemporary Price Trends and their Economic Significance in the Ashanti Region of
Ghana
James Fearon, Juliet Asare, Elijah O. Okran
Detection of Chlamydia trachomatis Immunogloblins in Seminal Plasma by
Microimmunofluorescence Test and Their Effect on Semen Parameters of Infertile Males
in Iraq
Ali Ibrahim Ali Al-Ezzy
PDF
2029
PDF
3037
PDF
3847
PDF
4853
Effect of Broccoli Flower Extract (Brassica oleracea L. var.italica Plenck) on Inhibition of PDF
Photoaging Viewed from Matrix Metalloproteinase-1 Expression in Human Skin Fibroblast
54Nelva K. Jusuf, Adang Bachtiar, Sumadio Hadisahputra, Hardyanto Soebono
59
Floristic Composition, Life Form and Chorology of Plant Life at Al-Saoda, Asir Region,
South-Western Saudi Arabia
Saadiya S. Seraj, Rahma N. Jrais, Sakeena K. Ayyad
PDF
6065
Effect of Heat and Cold Therapy during the First Stage of Labor on Women Perception of PDF
Birth Experience: A Randomized Controlled Trial
6672
Rania Mahmoud Abdel Ghani
Modes of Parasitism between the Necrotrophic Fungus Botrytis cinerea and Trichoderma
spp
PDF
7380
Hema Moorthy Ram, Shuchishweta V Kendurkar
Prevalence of abomasal nematodes in sheep and goats slaughtered at Haramaya municipal PDF
abattoir, eastern Hararghe, Ethiopia
81Shimelis Argaw, Desta Beyene, Belay Abebe
87
Correlates of Missed Nursing Care in Selected Medical Intensive Care Units, Egypt
Manar Ahmed Elbadawy Abd Elrehem, Magda Abd El Hamid Abd El Fatah, Yousria
Abd El Salam Seloma
PDF
88105
Perceived Health Effects of Environmental Noise Pollution on the Inhabitants of AdoEkiti Metropolis. Ekiti State, Nigeria
PDF
106113
Awosusi A.O, Akindutire I.O
Improving the Utilization of Sorghum Spent Grain (Pito Mash) in Broiler Diets by
Treating with Plantain Peels Ash Extract
PDF
Asare-Bediako Ankrah, Esther Abandoh-Sam, Samuel Nyantakyi
114118
Stress Levels in the Stabilization Phase of Schizophrenia Patients
Superida Ginting, Vita Camellia, Bahagia Loebis
PDF
119-123
The Biostatistical Technique to Diagnose the Extract Effect of the Black Tea (Camellia
sinensis L.) on the Staphylococcus aureus Isolated from Conjunctivitis
Hasan Yasien Touama, Faten Hasan Yasien
The Effect of Cocoa Pods Waste as a Growing Media Supplement on Productivity and
Nutrient Content of Brown Oyster Mushroom (Pleurotus cystidiosus)
Imam Mudakir, Utami Sri Hastuti, Fatchur Rohman, Abdul Gofur
PDF
124133
PDF
134140
Antifungal activity of medicinal plants extracts against Botrytis cinerea the causal agent PDF
of gray mold on tomato
141Btissam Senhaji, Bouchra Chebli, El Hassan Mayad, Zahra Ferji
147
Correlation of Paternal Homocysteine Level and Sperm DNA Fragmentation in Couple
with Idiopathic Recurrent Early Pregnancy Loss
Binarwan Halim, Delfi Lutan, M.Thamrin Tanjung, Ethiraj Balaji Prasath
PDF
148155
Assessment of Patient Satisfaction with Nursing Care at a Large Public Referral Hospital PDF
in Kenya
Titus Kipchumba Tarus, Judith Nekesa Mangeni, Jane C. Nyariki, Kennedy Nyongesa
156Simiyu, Milly C. Obel, Moses Mwangi Gitonga, Angela Albright
160
Fruit Consumption among Staff of Agricultural Based Institutions
Oyedele, O.O, I.B. Adeoye, I.O. Amao, K.M. Bamimore, A.A. Layade
Cytotoxic effect of the purified lectin from locally Isolate Acinetobacter baumannii on
Hep-2 tumor cell line
Alaa N. Mahammed Ali
Determination of the Existence and Distribution of HIV-I Chemokine Co-Receptor 5
Polymorphism in a Sampled Population from Kenya
Benadine Bukachi, Samuel Khamadi, Michael Gicheru, Purity Gacheri
Analysis of the Role of Women in Seed Security in Lamurde Local Government Area,
Adamawa State, Nigeria.
Bawa, D.B, Donye, A.O., Nuhu, H.S
Risperidone Effectiveness with Attentional in Schizophrenic Patients
Lenni C. Sihite, Mustafa Mahmud Amin, Bahagia Loebis
Comparison of Mild Cognitive Impairment Between the Older People with Diabetes
Mellitus and without Diabetes Melitus
Wijaya Taufik Tiji, Mustafa M Amin, Elmeida Effendy, Dharma Lindarto
Comparative effect haloperidol and quetiapine within positive symptoms for female
schizophrenic patient
Hanip Fahri, Vita Camellia, Bahagia Loebis
Editorial Board
Prof. Dr. Sanjay Kumar
Dept. of Biotechnology, Ministry of Science and Technology, India
Dr. Chiung Ting Chang
Maastricht University, Netherlands
Prof. Dr. Venus S. Solar
Manila Central University, Philippines
Dr. Eng. Rares Halbac-Cotoara-Zamfir
PDF
161-167
PDF
168173
PDF
174189
PDF
190195
PDF
196-198
PDF
199203
PDF
204209
"Politehnica" University of Timisoara Romania
Prof. Dr. P. Satheeshkumar
Central Marine Fisheries Research Institute, India
Prof. Dr.Ibrahim Hassan
Alexabdria University, Egypt
Prof. Dr.Jagruthi Joshi
Novartis Healthcare, India
Dr. Nabil Miled
Sfax University, Tunisia
Prof. Dr. Carlos K B Ferrari
Federal University of Mato Grosso (UFMT), Brazil
Prof. Dr. H. A. Ibrahim,
Suez Canal university, Egypt
Dr. Arda YILDIRIM
Gaziosmanpasa University, Turkey
Dr.Nexhbedin Beadini
Faculty of Medical Sciences, State University of Tetova, Macedonia
Dr.Sheqibe Beadini
Faculty of Medical Sciences, State University of Tetova, Macedonia
Dr.Nihad Abdulateef ali
Department of Animal production, College of Agriculture, AL-Qasim Green University-Iraq
Journal of Biology, Agriculture and Healthcare
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.26, 2014
www.iiste.org
Effect of Broccoli Flower Extract (Brassica oleracea L. var.italica
Plenck) on Inhibition of Photoaging Viewed from Matrix
Metalloproteinase-1 Expression in Human Skin Fibroblast
Nelva K. Jusuf1*, Adang Bachtiar2, Sumadio Hadisahputra3, Hardyanto Soebono4
1, Department of Dermatovenereology Faculty of Medicine University of Sumatera Utara, Indonesia.
Address : Bunga Lau No. 17 Medan, Sumatera Utara, Indonesia.
2 Faculty of Public Health University of Indonesia, Indonesia.
3 Faculty of Pharmacy University of Sumatera Utara, Indonesia.
4 Department of Dermatovenereology Faculty of Medicine University of Gadjah Mada , Indonesia.
* E-mail of corresponding author : nelva_jusuf@yahoo.com
Abstract
Objective : Ultraviolet irradiation is the major external factors that cause skin photoaging. Ultraviolet B (UVB)
irradiation induces the production of matrix metalloproteinase (MMP) by activating cellular signaling
transduction pathways, which are responsible for the degradation of collagen. The imbalance between collagen
degradation and synthesis play a major role in the formation of wrinkle of photoaging. Broccoli (Brassica
oleracea L. var. italica Plenck) is a crucciferae group vegetables which has a great amount of antioxidant.
The aim of this study is to prove whether broccoli flower extract (BFE) as an effective antiphotoaging agent by a
MMP-1 inhibitor action in vitro. Methods : An experimental (in vitro) study had been conducted using UVB
irradiated primary human skin fibroblast culture. We studied the MMP-1 inhibitory effects of BFE on
photoaging. The quantification of MMP-1 mRNA expression was done by Realtime RT-PCR while the protein
expression assay was done by ELISA. Results : Pretreatment with BFE decrease MMP-1 expression both at
MMP-1 mRNA and MMP-1 protein expression. There were significant differences of mean value of MMP-1
mRNA and MMP-1 protein expression between every group based on irradiation dose (p
Table of Contents
Articles
Histological observation of Gelam (Melaleuca cajuputi Powell) in different ecosystems of
Terengganu
M. Masitah, A.R. Shamsul Bahri, Jamilah M.S., Salwani Ismail
Challenges of Utilizing Agricultural Information Sources by Small Scale Farmers in
Nasarawa State, Nigeria
Bello M, C. P. O. Obinne, S. A. Bako
Circumcision Risks, Benefits and the Prospective
Sokol Buba
1-7
8-16
17-19
Comparative Antibiotic Activities of Ethanol Extracts of Leaves and Inflorescences of
Mitracarpus villosus from two different Geographical Regions in Nigeria in Synergy and
Antagonism with Conventional Antibiotics
Odima, C. A., Ubani, C. S., Eze, E. A., Oje, O. E., Agu, E. C., Joshua, P. E.
Comparative Study of Training Needs of Forestry Workers in Oyo and Osun States,
Nigeria
Ayandiji, A., Adekunle O.A.
Contemporary Price Trends and their Economic Significance in the Ashanti Region of
Ghana
James Fearon, Juliet Asare, Elijah O. Okran
Detection of Chlamydia trachomatis Immunogloblins in Seminal Plasma by
Microimmunofluorescence Test and Their Effect on Semen Parameters of Infertile Males
in Iraq
Ali Ibrahim Ali Al-Ezzy
2029
3037
3847
4853
Effect of Broccoli Flower Extract (Brassica oleracea L. var.italica Plenck) on Inhibition of PDF
Photoaging Viewed from Matrix Metalloproteinase-1 Expression in Human Skin Fibroblast
54Nelva K. Jusuf, Adang Bachtiar, Sumadio Hadisahputra, Hardyanto Soebono
59
Floristic Composition, Life Form and Chorology of Plant Life at Al-Saoda, Asir Region,
South-Western Saudi Arabia
Saadiya S. Seraj, Rahma N. Jrais, Sakeena K. Ayyad
6065
Effect of Heat and Cold Therapy during the First Stage of Labor on Women Perception of PDF
Birth Experience: A Randomized Controlled Trial
6672
Rania Mahmoud Abdel Ghani
Modes of Parasitism between the Necrotrophic Fungus Botrytis cinerea and Trichoderma
spp
7380
Hema Moorthy Ram, Shuchishweta V Kendurkar
Prevalence of abomasal nematodes in sheep and goats slaughtered at Haramaya municipal PDF
abattoir, eastern Hararghe, Ethiopia
81Shimelis Argaw, Desta Beyene, Belay Abebe
87
Correlates of Missed Nursing Care in Selected Medical Intensive Care Units, Egypt
Manar Ahmed Elbadawy Abd Elrehem, Magda Abd El Hamid Abd El Fatah, Yousria
Abd El Salam Seloma
88105
Perceived Health Effects of Environmental Noise Pollution on the Inhabitants of AdoEkiti Metropolis. Ekiti State, Nigeria
106113
Awosusi A.O, Akindutire I.O
Improving the Utilization of Sorghum Spent Grain (Pito Mash) in Broiler Diets by
Treating with Plantain Peels Ash Extract
Asare-Bediako Ankrah, Esther Abandoh-Sam, Samuel Nyantakyi
114118
Stress Levels in the Stabilization Phase of Schizophrenia Patients
Superida Ginting, Vita Camellia, Bahagia Loebis
119-123
The Biostatistical Technique to Diagnose the Extract Effect of the Black Tea (Camellia
sinensis L.) on the Staphylococcus aureus Isolated from Conjunctivitis
Hasan Yasien Touama, Faten Hasan Yasien
The Effect of Cocoa Pods Waste as a Growing Media Supplement on Productivity and
Nutrient Content of Brown Oyster Mushroom (Pleurotus cystidiosus)
Imam Mudakir, Utami Sri Hastuti, Fatchur Rohman, Abdul Gofur
124133
134140
Antifungal activity of medicinal plants extracts against Botrytis cinerea the causal agent PDF
of gray mold on tomato
141Btissam Senhaji, Bouchra Chebli, El Hassan Mayad, Zahra Ferji
147
Correlation of Paternal Homocysteine Level and Sperm DNA Fragmentation in Couple
with Idiopathic Recurrent Early Pregnancy Loss
Binarwan Halim, Delfi Lutan, M.Thamrin Tanjung, Ethiraj Balaji Prasath
148155
Assessment of Patient Satisfaction with Nursing Care at a Large Public Referral Hospital PDF
in Kenya
Titus Kipchumba Tarus, Judith Nekesa Mangeni, Jane C. Nyariki, Kennedy Nyongesa
156Simiyu, Milly C. Obel, Moses Mwangi Gitonga, Angela Albright
160
Fruit Consumption among Staff of Agricultural Based Institutions
Oyedele, O.O, I.B. Adeoye, I.O. Amao, K.M. Bamimore, A.A. Layade
Cytotoxic effect of the purified lectin from locally Isolate Acinetobacter baumannii on
Hep-2 tumor cell line
Alaa N. Mahammed Ali
Determination of the Existence and Distribution of HIV-I Chemokine Co-Receptor 5
Polymorphism in a Sampled Population from Kenya
Benadine Bukachi, Samuel Khamadi, Michael Gicheru, Purity Gacheri
Analysis of the Role of Women in Seed Security in Lamurde Local Government Area,
Adamawa State, Nigeria.
Bawa, D.B, Donye, A.O., Nuhu, H.S
Risperidone Effectiveness with Attentional in Schizophrenic Patients
Lenni C. Sihite, Mustafa Mahmud Amin, Bahagia Loebis
Comparison of Mild Cognitive Impairment Between the Older People with Diabetes
Mellitus and without Diabetes Melitus
Wijaya Taufik Tiji, Mustafa M Amin, Elmeida Effendy, Dharma Lindarto
Comparative effect haloperidol and quetiapine within positive symptoms for female
schizophrenic patient
Hanip Fahri, Vita Camellia, Bahagia Loebis
Editorial Board
Prof. Dr. Sanjay Kumar
Dept. of Biotechnology, Ministry of Science and Technology, India
Dr. Chiung Ting Chang
Maastricht University, Netherlands
Prof. Dr. Venus S. Solar
Manila Central University, Philippines
Dr. Eng. Rares Halbac-Cotoara-Zamfir
161-167
168173
174189
190195
196-198
199203
204209
"Politehnica" University of Timisoara Romania
Prof. Dr. P. Satheeshkumar
Central Marine Fisheries Research Institute, India
Prof. Dr.Ibrahim Hassan
Alexabdria University, Egypt
Prof. Dr.Jagruthi Joshi
Novartis Healthcare, India
Dr. Nabil Miled
Sfax University, Tunisia
Prof. Dr. Carlos K B Ferrari
Federal University of Mato Grosso (UFMT), Brazil
Prof. Dr. H. A. Ibrahim,
Suez Canal university, Egypt
Dr. Arda YILDIRIM
Gaziosmanpasa University, Turkey
Dr.Nexhbedin Beadini
Faculty of Medical Sciences, State University of Tetova, Macedonia
Dr.Sheqibe Beadini
Faculty of Medical Sciences, State University of Tetova, Macedonia
Dr.Nihad Abdulateef ali
Department of Animal production, College of Agriculture, AL-Qasim Green University-Iraq
Journal of Biology, Agriculture and Healthcare
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.26, 2014
www.iiste.org
Effect of Broccoli Flower Extract (Brassica oleracea L. var.italica
Plenck) on Inhibition of Photoaging Viewed from Matrix
Metalloproteinase-1 Expression in Human Skin Fibroblast
Nelva K. Jusuf1*, Adang Bachtiar2, Sumadio Hadisahputra3, Hardyanto Soebono4
1, Department of Dermatovenereology Faculty of Medicine University of Sumatera Utara, Indonesia.
Address : Bunga Lau No. 17 Medan, Sumatera Utara, Indonesia.
2 Faculty of Public Health University of Indonesia, Indonesia.
3 Faculty of Pharmacy University of Sumatera Utara, Indonesia.
4 Department of Dermatovenereology Faculty of Medicine University of Gadjah Mada , Indonesia.
* E-mail of corresponding author : nelva_jusuf@yahoo.com
Abstract
Objective : Ultraviolet irradiation is the major external factors that cause skin photoaging. Ultraviolet B (UVB)
irradiation induces the production of matrix metalloproteinase (MMP) by activating cellular signaling
transduction pathways, which are responsible for the degradation of collagen. The imbalance between collagen
degradation and synthesis play a major role in the formation of wrinkle of photoaging. Broccoli (Brassica
oleracea L. var. italica Plenck) is a crucciferae group vegetables which has a great amount of antioxidant.
The aim of this study is to prove whether broccoli flower extract (BFE) as an effective antiphotoaging agent by a
MMP-1 inhibitor action in vitro. Methods : An experimental (in vitro) study had been conducted using UVB
irradiated primary human skin fibroblast culture. We studied the MMP-1 inhibitory effects of BFE on
photoaging. The quantification of MMP-1 mRNA expression was done by Realtime RT-PCR while the protein
expression assay was done by ELISA. Results : Pretreatment with BFE decrease MMP-1 expression both at
MMP-1 mRNA and MMP-1 protein expression. There were significant differences of mean value of MMP-1
mRNA and MMP-1 protein expression between every group based on irradiation dose (p