Chemical Composition and Larvicidal Activities of essential oil of Piper betle from the Indonesian Plants against Aedes aegypti L.
PROCEEDING
TNTERNATToNAL
BtorEcHNoLoGy
AND5'nKBtcoNGREss
SEMTNAR
MAIANG,JULY27-29,2010
uNtvERslwoF MUHAMMADTYAH
Theme:
"Biotechnolory:Breakthroughfor the Futureof Industrial
in DevelopingCountries"
Challenges
ChiefEdior:
Suharsono
Editors:
KomangG. Wiryawan
BambangPrasetya
Tri Muii Ermawati
PuspitaLisdiyanti
SaptowoJ. Pardal
SiswaSetyahadi
AbuAmar
HariEkolrianto
WahyuPurbowasito
HayatiMinarsih
FerrySandra
Mlsri Gozan
SetiartlSukotio
Saidatultdlyah
ArisWinaya
Organized
by:
Centerfor Eiotechnology
Development,Universityof Muhammadiyah
Malangand
(KBl)
KonsorsiumBioteknologilndonesia
$
I
OF THE INTERNATIONAL
BIOTECHNOLOGY
SEMINAR
IPROCEEDING
AND5thKBrcoNGREss
JULY27-29,2010
fSBN: 978-979-769-158-9
Publishedby:
Development,
University
Centerfor Biotechnology
of Muhammadiyah
Malang
Jl RayaTlogomas
246,MalaoB,
€ mail:biotechsem@vahoo.com
(KBl)
Indonesia
andKonsorsium
Bioteknologi
Jl.RayaBogorKm46,Cibinong,
16911
Printedby:
AdityaMedia
Jl. Bimasaki No. 19 Yogyakarta-55281.
Tefp. (02741 52Oi,612,
520613
RESEARCHOF HEATTH
1. ASnesO.Uzandl
VascgrowEnhance
Progenitor
Endothelial
CellsProliferation
andDifferentiation
2. HenlngWU6watl
TheInfuenceof Cadmlumto the Amountof Vitamlnin AquatlcVegetables
3. Aneda Prasetyoputrl
TowardsNsu InfluenzaAntiviralfrom NaturalProducts:the Developmentof a Bioassay
Basedon
HeterologousExpression
of M2 lon{hannel Protein
4
ZesflnB.P
Statusof Reproductionof PostMortem KacangGoatin SlaughterHousein Padang
5. Zaltunl Udin
of Epididymis
Effectof StorageTemperature
on SemenQualityof SwampBuffalo
6. CamyF.SurJadl
Cryopreserved
UmbilicalCordBloodMononuclear
Cellasa PotentialSourcefor ClinicalApplication
7. DwiWahyuni
ChemicalCompositionand LarvlcidalActivitlesof EssentialOllof Piperbeflefrom the Indonesian
PlantsAgainst AedesaegyptiL
FajarSufyantoro
Potentialof GuanoBacterialsolatesto HelaCervixCancerCell
Cytotoxicand Apoptotic-causing
Nl Made Drrl turwadl
lmprovingthe Alkalistabilityof B Xylosidase
Perylz by SiteDirectedMutagenesis
BayylnatulMuchtaromah
TheEffectof Pegagan(C-entello
asiatica{1.)Urban}asMaterialAntifertilityto gpt(glutamicpyruvote
transominsse)and got lglutamic oxoloscetictronsominase)Concentrationand tts HistologicalProfile
of FemafeMice (Mus musculusl
laura Wijaya
CurcumodomestlcoUnn EthanolExtractEnhanceMesenchymal
StemCellExpansion
RlrnaTrianaDewl
Productionof Lovastatin
andSulochrinby Aspergillus
terreusUsingSolidStateFermentation
13. AgusMulyono
Detectionof EyeDefectsWith EyelmageTextureAnalysis
14. Widodo
Functional
Genomic-based
Screening,
MolecularPathwayAnalysis
andApplication
Systems
Biologyin
TraditionalMedicine
Muhaimin Rifai
Therapyof AutoimmuneRheumatoid
ArthritisWithT CellsCd4'Cd25'
Regulatory
&
tc
t
I
16. DlonlDjunaedl
TheRoleof Biotechnology
In DengueViralInfection:Effortfor the Development
of A DengueVaccine
L7. EstrlLarasArumlnttyat
IntergenicSpacerldentificationof Porangin EastJava
18. ArlsWinaya
Variationof lnterleukin-8(lt-8) ReceptorGeneBasedon SingleStrandConformationPolymorphism
(SSCP)
in lndonesianFrisienHolsteinDairyCattleat MalangRegency
19. PoncolarlWahyono
Effectof Extnct from Tomato(tycopersicumpyriforme)Fruiton the Activityof mmpl and Lipid
Peroxidationon AgingSkin
Ull Zallzar
Effectiveness
OintmentBetleLeavesand Meniranto Reductionof Total Bacteriatn Milk of Sub
ClinicalMastitisDairyCow
21. HawlnnurDiana
on BoneRemodlingin
Effectof SolpeanNutrition on ReceptorEstrogen(ER)c Expression
Rat(Rattusnoruegicusl
OsteoporosisRiskStudyon Ovariectomized
Decreasing
I N T E RN A T , o I { A
L
andLarvicidalActil'itiesof cssentialoil of Piper
Chemicalcomposition
:: I'.
Plantsagaif,rtfiwdpgleppt!\
betlefrom the Indonesian
\ _ e n g_r e-n':
ss
DWIWA[II'UNI
Dcpartment of Biology,Faculty of TeacherTraining and Education-University of
Jember,Indonesian
Mav.20I0
Abstract
Aedes aegypti are the most important of insects n,ell-knou'ufbr their public health
importauce,since they act as vector for many tropical and subtrnpicaldiseasessuch as
denguefever, yellow fever. Aedes aegyptiL. is thc ntu.jot'r'cc:lot'o.f tlengte .fl:ver,un
endemicdi,seasein Indonesian.In vierv of the recentlyincreasedinterestin developing
plant or;gin insecticitlesas an altemativeto chemical insecticide,this srudy rvas
undertaxcnto assessthe larvicidalpotentialof the essentialoil colnpositionch, mical o1'
plantsagainstmedicallyimportantspeciesof rnosquito
Piper betle lrom the lnclonesian
oil u,asextractedby stearrtdistillation
The essential
L'.
Methods:
vectors,Aedesaegypti
couple to mass
and iherr chernicalcompositiondeterminedby GL -Chrornatography
oil contpositionchcrnicalwa-sevaluateduntler
Bioef'licacyof the essentiul
spectroscopy.
liibclrrtoryconditionst'sing IIt - IV instarsAedes .regyplilarvae.rvereanalyzedby
of their LC50. Results:Among the cheruicalc(rnl[rsitionof the essentirl
medsu:'€'ment
oil ale eugenol,kavikol and estragol,eugenolrvasthe most sensitive(LC -5t): "10.6
ppm), followed by kavikol (54, 9 ppm) and estragol(57. 6 ppm). Interpretation&
Conclu,rion:From the results it can be conclucledthat lbe Aedes aegl'pti litrvae was
airniirg
to the eugenol.Suchlindingswould be uselulin pronrotingresearcrt
susceptible
laryae chernicalcotnpounclsfrotn
at tlre developrnentol'nerv agent for Aedesaeg.t'trtti
'ndigeiro'-rs
on bioactive
plant sourcesasan altemativeto chenricallarvisides.based
oils - eugenol,dengue
Key rvolds: larvicidalactivity- Aetlesaeggoti- essential
#
f
$
/
fi
if
la
ri.
i
(
TNTERNATToNAL
BtorEcHNoLoGy
AND5'nKBtcoNGREss
SEMTNAR
MAIANG,JULY27-29,2010
uNtvERslwoF MUHAMMADTYAH
Theme:
"Biotechnolory:Breakthroughfor the Futureof Industrial
in DevelopingCountries"
Challenges
ChiefEdior:
Suharsono
Editors:
KomangG. Wiryawan
BambangPrasetya
Tri Muii Ermawati
PuspitaLisdiyanti
SaptowoJ. Pardal
SiswaSetyahadi
AbuAmar
HariEkolrianto
WahyuPurbowasito
HayatiMinarsih
FerrySandra
Mlsri Gozan
SetiartlSukotio
Saidatultdlyah
ArisWinaya
Organized
by:
Centerfor Eiotechnology
Development,Universityof Muhammadiyah
Malangand
(KBl)
KonsorsiumBioteknologilndonesia
$
I
OF THE INTERNATIONAL
BIOTECHNOLOGY
SEMINAR
IPROCEEDING
AND5thKBrcoNGREss
JULY27-29,2010
fSBN: 978-979-769-158-9
Publishedby:
Development,
University
Centerfor Biotechnology
of Muhammadiyah
Malang
Jl RayaTlogomas
246,MalaoB,
€ mail:biotechsem@vahoo.com
(KBl)
Indonesia
andKonsorsium
Bioteknologi
Jl.RayaBogorKm46,Cibinong,
16911
Printedby:
AdityaMedia
Jl. Bimasaki No. 19 Yogyakarta-55281.
Tefp. (02741 52Oi,612,
520613
RESEARCHOF HEATTH
1. ASnesO.Uzandl
VascgrowEnhance
Progenitor
Endothelial
CellsProliferation
andDifferentiation
2. HenlngWU6watl
TheInfuenceof Cadmlumto the Amountof Vitamlnin AquatlcVegetables
3. Aneda Prasetyoputrl
TowardsNsu InfluenzaAntiviralfrom NaturalProducts:the Developmentof a Bioassay
Basedon
HeterologousExpression
of M2 lon{hannel Protein
4
ZesflnB.P
Statusof Reproductionof PostMortem KacangGoatin SlaughterHousein Padang
5. Zaltunl Udin
of Epididymis
Effectof StorageTemperature
on SemenQualityof SwampBuffalo
6. CamyF.SurJadl
Cryopreserved
UmbilicalCordBloodMononuclear
Cellasa PotentialSourcefor ClinicalApplication
7. DwiWahyuni
ChemicalCompositionand LarvlcidalActivitlesof EssentialOllof Piperbeflefrom the Indonesian
PlantsAgainst AedesaegyptiL
FajarSufyantoro
Potentialof GuanoBacterialsolatesto HelaCervixCancerCell
Cytotoxicand Apoptotic-causing
Nl Made Drrl turwadl
lmprovingthe Alkalistabilityof B Xylosidase
Perylz by SiteDirectedMutagenesis
BayylnatulMuchtaromah
TheEffectof Pegagan(C-entello
asiatica{1.)Urban}asMaterialAntifertilityto gpt(glutamicpyruvote
transominsse)and got lglutamic oxoloscetictronsominase)Concentrationand tts HistologicalProfile
of FemafeMice (Mus musculusl
laura Wijaya
CurcumodomestlcoUnn EthanolExtractEnhanceMesenchymal
StemCellExpansion
RlrnaTrianaDewl
Productionof Lovastatin
andSulochrinby Aspergillus
terreusUsingSolidStateFermentation
13. AgusMulyono
Detectionof EyeDefectsWith EyelmageTextureAnalysis
14. Widodo
Functional
Genomic-based
Screening,
MolecularPathwayAnalysis
andApplication
Systems
Biologyin
TraditionalMedicine
Muhaimin Rifai
Therapyof AutoimmuneRheumatoid
ArthritisWithT CellsCd4'Cd25'
Regulatory
&
tc
t
I
16. DlonlDjunaedl
TheRoleof Biotechnology
In DengueViralInfection:Effortfor the Development
of A DengueVaccine
L7. EstrlLarasArumlnttyat
IntergenicSpacerldentificationof Porangin EastJava
18. ArlsWinaya
Variationof lnterleukin-8(lt-8) ReceptorGeneBasedon SingleStrandConformationPolymorphism
(SSCP)
in lndonesianFrisienHolsteinDairyCattleat MalangRegency
19. PoncolarlWahyono
Effectof Extnct from Tomato(tycopersicumpyriforme)Fruiton the Activityof mmpl and Lipid
Peroxidationon AgingSkin
Ull Zallzar
Effectiveness
OintmentBetleLeavesand Meniranto Reductionof Total Bacteriatn Milk of Sub
ClinicalMastitisDairyCow
21. HawlnnurDiana
on BoneRemodlingin
Effectof SolpeanNutrition on ReceptorEstrogen(ER)c Expression
Rat(Rattusnoruegicusl
OsteoporosisRiskStudyon Ovariectomized
Decreasing
I N T E RN A T , o I { A
L
andLarvicidalActil'itiesof cssentialoil of Piper
Chemicalcomposition
:: I'.
Plantsagaif,rtfiwdpgleppt!\
betlefrom the Indonesian
\ _ e n g_r e-n':
ss
DWIWA[II'UNI
Dcpartment of Biology,Faculty of TeacherTraining and Education-University of
Jember,Indonesian
Mav.20I0
Abstract
Aedes aegypti are the most important of insects n,ell-knou'ufbr their public health
importauce,since they act as vector for many tropical and subtrnpicaldiseasessuch as
denguefever, yellow fever. Aedes aegyptiL. is thc ntu.jot'r'cc:lot'o.f tlengte .fl:ver,un
endemicdi,seasein Indonesian.In vierv of the recentlyincreasedinterestin developing
plant or;gin insecticitlesas an altemativeto chemical insecticide,this srudy rvas
undertaxcnto assessthe larvicidalpotentialof the essentialoil colnpositionch, mical o1'
plantsagainstmedicallyimportantspeciesof rnosquito
Piper betle lrom the lnclonesian
oil u,asextractedby stearrtdistillation
The essential
L'.
Methods:
vectors,Aedesaegypti
couple to mass
and iherr chernicalcompositiondeterminedby GL -Chrornatography
oil contpositionchcrnicalwa-sevaluateduntler
Bioef'licacyof the essentiul
spectroscopy.
liibclrrtoryconditionst'sing IIt - IV instarsAedes .regyplilarvae.rvereanalyzedby
of their LC50. Results:Among the cheruicalc(rnl[rsitionof the essentirl
medsu:'€'ment
oil ale eugenol,kavikol and estragol,eugenolrvasthe most sensitive(LC -5t): "10.6
ppm), followed by kavikol (54, 9 ppm) and estragol(57. 6 ppm). Interpretation&
Conclu,rion:From the results it can be conclucledthat lbe Aedes aegl'pti litrvae was
airniirg
to the eugenol.Suchlindingswould be uselulin pronrotingresearcrt
susceptible
laryae chernicalcotnpounclsfrotn
at tlre developrnentol'nerv agent for Aedesaeg.t'trtti
'ndigeiro'-rs
on bioactive
plant sourcesasan altemativeto chenricallarvisides.based
oils - eugenol,dengue
Key rvolds: larvicidalactivity- Aetlesaeggoti- essential
#
f
$
/
fi
if
la
ri.
i
(