B. SIMPULAN DAN SARAN AKTIVITAS ANTIFUNGI EKSTRAK TANAMAN OBAT NAN FEI SHU TERHADAP Candida albicans.
B. SIMPULAN DAN SARAN
A. Simpulan
1.
Ekstrak tanaman obat Nan Fei Shu tidak memiliki aktivitas
antifungi terhadap Candida albicans.
2. Penamaan ilmiah tanaman obat Nan Fei Shu yakni Vernonia
amygdalina atau Gymnanthemum amygdalinum.
B. Saran
de perlu dilakukan
dilaku
kuka
k n isolasi senyawa fitol dan
1. Ekstrak crud
crude
neophytadiene
neophy
yta
tadienee ddengan
engan kkromatografi
roma
ro
mato
t gr
g affi cair seperti HPLC untuk
men
nguj
ujii potensi
pote
po
tensi senyawa tersebut
terseb
but
u dalam
dal
alam
m menghambat
m
menguji
Candida
al
lbica
cans
ns.
albicans.
Kult
Ku
ltur
u yang digunakan untuk pengujian
peng
nguj
ujiaan aktivitas antifungi
2. Kultur
dire
reko
k mendasikan berasal
ber
eras
asal dari genuinee Am
direkomendasikan
American Type Culture
Coll
Collection
lec
ecti
tion
o dan
dan sselanjutnya
elan
el
anju
jutn
tnya
ya penggunaan
pen
engg
ggunaa
aan
n kul
kultur maksimal berasal
genuine
American
Culture Collection (ATCC)
dari subkultur genui
ine Am
merican Type Cultu
yang kelima.
3. Ekstraksi perlu dilakukan dari bahan simplisia segar menggunakan
metode perkolasi dengan pelarut non polar seperti petroleum eter
dan heksana terhadap Aspergillus niger, Aspergillus fumigatus, dan
Aspergillus flavus.
47
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Revi
LAMPIRAN 1
Gambar 9. Tanaman obat Nan Fei Shu Gambar 10
10.
0. Pena
Pe
Penampang
nam
m
daun tanaman
obat
Nan Fei Shu
ob
bat Na
Gambar 11. Beragam ukuran daun tanaman obat Nan Fei Shu dari termuda
(kanan) hingga sebelum layu (kiri) diatas kertas berukuran A4.
58
59
A
B
Gambar 11. Uji
ji zona hambat pad
pada
adaa ko
konsentrasi 200 mg/m
mg/ml. (A) pengulangan
I,II,III;
,III; (B) pengulangan
penggul
ulaangan IV, V
A
B
Gambar 12. Uji
ji zona hambat pada
pad
da ko
konsentrasi
onsentrasi 100 mg/m
mg/ml. (A) pengulangan
I,II,III;
pengulangan
IV,
,III; (B) pengulanga
an IV
V, V
A
B
Gambar 13. Uji zona hambat pada konsentrasi 50 mg/ml. (A) pengulangan
I,II,III; (B) pengulangan IV, V
60
A
B
Gambar 14. Uji
ji zona hambat ppada
adaa ko
ad
konsentrasi 25 mg/m
mg/ml. (A) pengulangan
I,II,III;
,III; (B) pengulangan
penggul
ulaangan IV, V
A
B
Gambar 15. Uji
ji zona hambat pada
pad
da ko
kontrol
ontrol negatif, DMS
DMSO (A) pengulangan
I,II,III;
pengulangan
IV,
,III; (B) pengulanga
an IV
V, V
I
III
II
IV
V
A
B
Gambar 16. Uji zona hambat pada kontrol positif, ketoconazole (A) pengulangan
I,II,III; (B) pengulangan IV, V
LAMPIRAN 2
Tabel 3. Hasil identifikasi KG-SM ekstrak tanaman obat Nan Fei Shu
Puncak Waktu
Komponen
Persentase
keRetensi
(%)
(menit)
23
16,694 phytol
15,397
36
24,244 heneicosane
11,009
18
13,997 neophytadiene
9,885
22
15,33 hexadecanoic acid
8,959
24
17,026 9,12-octadecadienoic acid
8,571
32
22,786 nonacosane
4,591
14
13,328 cis-2-ethyl-2-hexen-1-ol
3,508
42
26,841 chondril
chondrillasterol
lla
last
ster
erol
ol
3,015
20
14,438 neop
neophytadiene
oph
hytadiene
2,962
15
13,552 ppentacosane
entacosan
anee
2,882
16
13,633 (7
(7e)-1,8-dimethyl-7-dodecenyl
7e))-1,
1,8
8-di
d meth
thyl
yl--7-dode
d ceny
nyl ace
acetate
2,328
17
13,782
13,7
782 pl
pluchidiol
pluc
uchi
hid
diol
1,951
41
26,427
26
6,427
27 22-methyl-7-phenylindole
-methyl-7-phenylindol
olee
1,872
40
226,185
6,1185 2-ethylacridine
1,728
19
114,249
4,2
,249
49 neophytadiene
1,486
37
24,7655 alpha -tocopherol
24
1,26
3-buten-2-one,4-(1,3,3-trimethyl-73-buten-2-one,4-(1,3,3-trime
ethy
hyl-7
7
oxabicyclo[4.1.0]hep-2-yl)oxab
ox
abic
icyc
yclo
lo[4
[4.11.0
.0]h
]hep
ep-2
2-yl
y )3
9,859
9,85
9,
859 ,[1.alpha.,2.b
,[1.alpha.,2.beta.(z),6.alpha.]
beta.(z),6.alpha.]
1,198
29
22,36
22,3
22
,366 1,
1,5,9-undecatriene,2,6,10-trimethyl1,5,
5,99-un
unde
deca
catr
trie
iene
ne,2
,2,6
,6,1
,10
0-tri
rime
meth
thy
1,137
39
25,623
25,6
623 me
methyl
meth
thyl
yl (4-tert-butylphenoxy)
(4-tert
rt-bu
butylp
lphe
heno
noxy
xy)) acetate
aceta
1,098
46
28,058 1,2-bis(trim
1,2-bis(trimethylsilyl)benzene
methy
hylsilyl)benzene
1,094
48
29,772 1,2-bis(trim
1,2-bis(trimethylsilyl)benzene
methy
hylsilyl)benzene
1,092
13
12,842 3,6-octadien
3,6-octadien-1-ol,3,7-dimethylen-1
1-ol,3,7-dimethyl0,876
35
24,088 beta -tocoph
-tocopherol
her
e ol
0,86
49
33,566 2-ethylacridine
0,799
10
12,19 chloromethyl 7-chlorononanoate
0,745
47
29,01 1,2-bis(trimethylsilyl)benzene
0,74
1-(2-methylcyclopent-1-enyl)-1-hydroxy9
11,655 2-propene
0,69
11
12,365 (trans)-2-azidocyclopentan-1-ol
0,689
21
14,85 triacontane
0,627
34
23,468 eicosane
0,608
n-cyano-n',n',n'',n''-tetramethyl-1,3,550
33,884 triazinetriamine
0,577
25
17,607 5-nitrouracil
0,452
12
12,615 nonadecane
0,427
2
9,204 1,4-diethylhexyl methoxyacetate
0,379
45
27,658 benzo[h]quinoline, 2,4-dimethyl0,379
61
62
6
8
43
27
7
10,861
11,531
27,082
21,386
10,961
26
31
33
1
5
44
20,353
22,614
23,304
9,033
10,127
27,243
4
28
10,071
22,098
30
38
22,4
22,471
,471
7
25,109
25
5,109
09
2(4h)-benzofuranone, 5,6,7,7a-tetrahydro4,4,7a-trimethyl2-piperidinone
2-ethylacridine
eicosane
propane
1-naphthalenamine-5,8-13c2,5,6,7tetrahydro2-methyl-7-phenylindole
2-ethylacridine
2,3,5,-trimethylhexane
nonadecane
cyclotrisiloxane,
y
hexamethyly
1,5-dimethylcyclohexene-51,5-di
ime
metthylcyclloh
ohex
e ene-5carbocaldehyde
ca
arb
rbocalde
d hyde
22-nonadecanone
-noona
nadeca
cano
none
ne
benzoic
benz
be
zoi
oicc ac
acid
acid,2,4-dimethoxy-6-propyl-,4id,22,4-di
4 dime
m th
hox
o y-6
6-prop
carboxy-3-hydroxy-5-propylphenyl
carb
ca
rboxy-3-hydroxy-5
5-pr
p op
opyl
ylph
phen
e y ester
44'methyl-2phenylindole
'methyl-2phenylindole
0,358
0,338
0,328
0,319
0,318
0,316
0,306
0,304
0,286
0,263
0,248
0,243
0,22
0,142
0,14
LAMPIRAN 3
Tabel 4. Hasil analisis variasi (ANOVA) luas zona hambat aktivitas antifungal
variasi konsentrasi ekstrak tanaman obat Nan Fei Shu, ketoconazole 1%
(b/v), dan DMSO terhadap Candida albicans.
Jumlah
df
Rerata
F
Sig.
(cm2)
(cm2)
Antar
546,165
5
109,233
274,600
,000
Kelompok
Dalam
9,547
24
0,398
Kelompok
Total
555,712
29
Tabel 5. Hasil Duncan
uncan letak beda
da nnyata
yata luas zo
zona hambat aktiv
aktivitas antifungal variasi
konsentrasi
1% (b/v), dan
trasi ekstrak
k ttanaman
anamaan obat Nan Fei
Fe Shu, ketoconazole
keto
DMSO terhadap
terhadaap Candida
Cand
ndid
ida albicans.
albi
al
bica
cans
ns.
,a
Duncan
Perlakuan
N
Subset
S
ub untuk alfa = 0,05
1
2
DMSO
5
,000000
200 mg/ml
5
,000000
100 mg/ml
5
,000000
50 mg/ml
5
,000000
,000
00000
0 0
25 mg/ml
5
,000000
Ketoconazole 1%
1% 5
11,449000
b )
( /v
Sig.
1,000
0
1,000
Rerata dari grup pada subset yang hhomogen
omog
gen ditampilkan.
a. Penggunaan
5,000
aan rerata ukuran sampel
sam
ampeel yang selaras = 5,00
63
LAMPIRAN 4
64
Gambar 17. Kromatogram Hasil Kromatogram Cair Ekstrak
LAMPIRAN 5
65
Gambar 18. Hasil alignment sekuens hasil sekuensing dengan sekuens Gymananthemum
amygdalinum yang diperoleh dari NCBI Genbank
LAMPIRAN 6
Gambar 19. Kromatogram Sekuens DNA menurut Primer ITS 1
66
LAMPIRAN 7
Gambar
Sekuens
ar 20. Kromatogram S
ek
kuens DNA menurut Primer ITS 4
67
LAMPIRAN 8
68
69
70
Gamb
mbar
ar 221.
1. B
LAST
LA
ST Sekuens
Sek
kuenss D
NA Nan
Nan Fei
Fe Shu
Gambar
BLAST
DNA
LAMPIRAN 9
Sekuens Gymnanthemum amygdalinum yang diperoleh dari NCBI GenBank
(fasta format)
>gi|40748098|gb|AY504695.1| Gymnanthemum amygdalinum internal transcribed
spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete
sequence
TCGATTAAAGCATTTCAGAATAACCTGTGAACATGTATTATTAT
TTGGGTGTTGGGAGGACGGGCTAATGCTCTCACCCTCTTTGCAT
CGTGTTGACATACACTTGTATAGCCTCTTWTTGGGTCGTACATG
TGTCTTGTTAGCATTTAAACAAACCCCCGGCACAGAACGTGCCA
C
C
GGG GCG C G
AGGATGAACAAAACATTAAAAGGGTGCGACTTGTGATGCCCCG
TATGCATTC
CAG
AGGTCGTG
GGC
G TTTTTTGT
TTCGCGGTATGCATTCAGGTCGTGGCTTTTTTGTAATTACAAAC
GGCAACG
CGG
GATA
ATCTCGGCT
TCA
CACGCAT
GACTCTCGGCAACGGATATCTCGGCTCACGCATCGATGAAGAA
AAAATG
TGCGAT
ATAC
ACTT
TTGG
GGTG
TGTG
T AA
ATT
T GCA
CGTAGCAAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGA
AGT
TTT
TTTT
TTGA
GAAC
ACG
GCAA
AGT
GTTG
T CG
CGCC
CCCG
C A
ACCATCGAGTTTTTGAACGCAAGTTGCGCCCGAAGCCATTTGGT
CACG
CGTC
TCTG
TGC
CCTGGGCGTCAC
CGC
GCAT
ATTG
TGC
CGAGGGCACGTCTGCCTGGGCGTCACGCATTGCATCGCCTCCTT
TCC
CCTT
TTC
CCTTAGTAGGCTTGTTGT
GTTC
TCGG
GG
CAATGCCTCCTTCCTTAGTAGGCTTGTTGTTCGGGGCGGAGATT
CAT
ATGC
GCTGATGGTGTGGTTGGCCT
CTAA
AAA
A
GGTCTCCCATGCTGATGGTGTGGTTGGCCTAAACGTAACTCCCT
GATA
ACATGACTAGTGGTGGTTGA
ACA
AA
TTCGGTGGATACATGACTAGTGGTGGTTGACAAGACCTTCGTTT
TGT
GTG
GTCGTTAACCGTAAGGGAAA
AAGG
GGT
T
GGAGTTGTGTGTCGTTAACCGTAAGGGAAAGGTTGTAAAAATC
GAGTC
CGT
TCT
CTTA
TATG
TGAT
ATGA
GACG
CGCT
CTTC
CGA
A
CCTTAATGAGTCGTCTTATGATGACGCTTCGA
71
A. Simpulan
1.
Ekstrak tanaman obat Nan Fei Shu tidak memiliki aktivitas
antifungi terhadap Candida albicans.
2. Penamaan ilmiah tanaman obat Nan Fei Shu yakni Vernonia
amygdalina atau Gymnanthemum amygdalinum.
B. Saran
de perlu dilakukan
dilaku
kuka
k n isolasi senyawa fitol dan
1. Ekstrak crud
crude
neophytadiene
neophy
yta
tadienee ddengan
engan kkromatografi
roma
ro
mato
t gr
g affi cair seperti HPLC untuk
men
nguj
ujii potensi
pote
po
tensi senyawa tersebut
terseb
but
u dalam
dal
alam
m menghambat
m
menguji
Candida
al
lbica
cans
ns.
albicans.
Kult
Ku
ltur
u yang digunakan untuk pengujian
peng
nguj
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dire
reko
k mendasikan berasal
ber
eras
asal dari genuinee Am
direkomendasikan
American Type Culture
Coll
Collection
lec
ecti
tion
o dan
dan sselanjutnya
elan
el
anju
jutn
tnya
ya penggunaan
pen
engg
ggunaa
aan
n kul
kultur maksimal berasal
genuine
American
Culture Collection (ATCC)
dari subkultur genui
ine Am
merican Type Cultu
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LAMPIRAN 1
Gambar 9. Tanaman obat Nan Fei Shu Gambar 10
10.
0. Pena
Pe
Penampang
nam
m
daun tanaman
obat
Nan Fei Shu
ob
bat Na
Gambar 11. Beragam ukuran daun tanaman obat Nan Fei Shu dari termuda
(kanan) hingga sebelum layu (kiri) diatas kertas berukuran A4.
58
59
A
B
Gambar 11. Uji
ji zona hambat pad
pada
adaa ko
konsentrasi 200 mg/m
mg/ml. (A) pengulangan
I,II,III;
,III; (B) pengulangan
penggul
ulaangan IV, V
A
B
Gambar 12. Uji
ji zona hambat pada
pad
da ko
konsentrasi
onsentrasi 100 mg/m
mg/ml. (A) pengulangan
I,II,III;
pengulangan
IV,
,III; (B) pengulanga
an IV
V, V
A
B
Gambar 13. Uji zona hambat pada konsentrasi 50 mg/ml. (A) pengulangan
I,II,III; (B) pengulangan IV, V
60
A
B
Gambar 14. Uji
ji zona hambat ppada
adaa ko
ad
konsentrasi 25 mg/m
mg/ml. (A) pengulangan
I,II,III;
,III; (B) pengulangan
penggul
ulaangan IV, V
A
B
Gambar 15. Uji
ji zona hambat pada
pad
da ko
kontrol
ontrol negatif, DMS
DMSO (A) pengulangan
I,II,III;
pengulangan
IV,
,III; (B) pengulanga
an IV
V, V
I
III
II
IV
V
A
B
Gambar 16. Uji zona hambat pada kontrol positif, ketoconazole (A) pengulangan
I,II,III; (B) pengulangan IV, V
LAMPIRAN 2
Tabel 3. Hasil identifikasi KG-SM ekstrak tanaman obat Nan Fei Shu
Puncak Waktu
Komponen
Persentase
keRetensi
(%)
(menit)
23
16,694 phytol
15,397
36
24,244 heneicosane
11,009
18
13,997 neophytadiene
9,885
22
15,33 hexadecanoic acid
8,959
24
17,026 9,12-octadecadienoic acid
8,571
32
22,786 nonacosane
4,591
14
13,328 cis-2-ethyl-2-hexen-1-ol
3,508
42
26,841 chondril
chondrillasterol
lla
last
ster
erol
ol
3,015
20
14,438 neop
neophytadiene
oph
hytadiene
2,962
15
13,552 ppentacosane
entacosan
anee
2,882
16
13,633 (7
(7e)-1,8-dimethyl-7-dodecenyl
7e))-1,
1,8
8-di
d meth
thyl
yl--7-dode
d ceny
nyl ace
acetate
2,328
17
13,782
13,7
782 pl
pluchidiol
pluc
uchi
hid
diol
1,951
41
26,427
26
6,427
27 22-methyl-7-phenylindole
-methyl-7-phenylindol
olee
1,872
40
226,185
6,1185 2-ethylacridine
1,728
19
114,249
4,2
,249
49 neophytadiene
1,486
37
24,7655 alpha -tocopherol
24
1,26
3-buten-2-one,4-(1,3,3-trimethyl-73-buten-2-one,4-(1,3,3-trime
ethy
hyl-7
7
oxabicyclo[4.1.0]hep-2-yl)oxab
ox
abic
icyc
yclo
lo[4
[4.11.0
.0]h
]hep
ep-2
2-yl
y )3
9,859
9,85
9,
859 ,[1.alpha.,2.b
,[1.alpha.,2.beta.(z),6.alpha.]
beta.(z),6.alpha.]
1,198
29
22,36
22,3
22
,366 1,
1,5,9-undecatriene,2,6,10-trimethyl1,5,
5,99-un
unde
deca
catr
trie
iene
ne,2
,2,6
,6,1
,10
0-tri
rime
meth
thy
1,137
39
25,623
25,6
623 me
methyl
meth
thyl
yl (4-tert-butylphenoxy)
(4-tert
rt-bu
butylp
lphe
heno
noxy
xy)) acetate
aceta
1,098
46
28,058 1,2-bis(trim
1,2-bis(trimethylsilyl)benzene
methy
hylsilyl)benzene
1,094
48
29,772 1,2-bis(trim
1,2-bis(trimethylsilyl)benzene
methy
hylsilyl)benzene
1,092
13
12,842 3,6-octadien
3,6-octadien-1-ol,3,7-dimethylen-1
1-ol,3,7-dimethyl0,876
35
24,088 beta -tocoph
-tocopherol
her
e ol
0,86
49
33,566 2-ethylacridine
0,799
10
12,19 chloromethyl 7-chlorononanoate
0,745
47
29,01 1,2-bis(trimethylsilyl)benzene
0,74
1-(2-methylcyclopent-1-enyl)-1-hydroxy9
11,655 2-propene
0,69
11
12,365 (trans)-2-azidocyclopentan-1-ol
0,689
21
14,85 triacontane
0,627
34
23,468 eicosane
0,608
n-cyano-n',n',n'',n''-tetramethyl-1,3,550
33,884 triazinetriamine
0,577
25
17,607 5-nitrouracil
0,452
12
12,615 nonadecane
0,427
2
9,204 1,4-diethylhexyl methoxyacetate
0,379
45
27,658 benzo[h]quinoline, 2,4-dimethyl0,379
61
62
6
8
43
27
7
10,861
11,531
27,082
21,386
10,961
26
31
33
1
5
44
20,353
22,614
23,304
9,033
10,127
27,243
4
28
10,071
22,098
30
38
22,4
22,471
,471
7
25,109
25
5,109
09
2(4h)-benzofuranone, 5,6,7,7a-tetrahydro4,4,7a-trimethyl2-piperidinone
2-ethylacridine
eicosane
propane
1-naphthalenamine-5,8-13c2,5,6,7tetrahydro2-methyl-7-phenylindole
2-ethylacridine
2,3,5,-trimethylhexane
nonadecane
cyclotrisiloxane,
y
hexamethyly
1,5-dimethylcyclohexene-51,5-di
ime
metthylcyclloh
ohex
e ene-5carbocaldehyde
ca
arb
rbocalde
d hyde
22-nonadecanone
-noona
nadeca
cano
none
ne
benzoic
benz
be
zoi
oicc ac
acid
acid,2,4-dimethoxy-6-propyl-,4id,22,4-di
4 dime
m th
hox
o y-6
6-prop
carboxy-3-hydroxy-5-propylphenyl
carb
ca
rboxy-3-hydroxy-5
5-pr
p op
opyl
ylph
phen
e y ester
44'methyl-2phenylindole
'methyl-2phenylindole
0,358
0,338
0,328
0,319
0,318
0,316
0,306
0,304
0,286
0,263
0,248
0,243
0,22
0,142
0,14
LAMPIRAN 3
Tabel 4. Hasil analisis variasi (ANOVA) luas zona hambat aktivitas antifungal
variasi konsentrasi ekstrak tanaman obat Nan Fei Shu, ketoconazole 1%
(b/v), dan DMSO terhadap Candida albicans.
Jumlah
df
Rerata
F
Sig.
(cm2)
(cm2)
Antar
546,165
5
109,233
274,600
,000
Kelompok
Dalam
9,547
24
0,398
Kelompok
Total
555,712
29
Tabel 5. Hasil Duncan
uncan letak beda
da nnyata
yata luas zo
zona hambat aktiv
aktivitas antifungal variasi
konsentrasi
1% (b/v), dan
trasi ekstrak
k ttanaman
anamaan obat Nan Fei
Fe Shu, ketoconazole
keto
DMSO terhadap
terhadaap Candida
Cand
ndid
ida albicans.
albi
al
bica
cans
ns.
,a
Duncan
Perlakuan
N
Subset
S
ub untuk alfa = 0,05
1
2
DMSO
5
,000000
200 mg/ml
5
,000000
100 mg/ml
5
,000000
50 mg/ml
5
,000000
,000
00000
0 0
25 mg/ml
5
,000000
Ketoconazole 1%
1% 5
11,449000
b )
( /v
Sig.
1,000
0
1,000
Rerata dari grup pada subset yang hhomogen
omog
gen ditampilkan.
a. Penggunaan
5,000
aan rerata ukuran sampel
sam
ampeel yang selaras = 5,00
63
LAMPIRAN 4
64
Gambar 17. Kromatogram Hasil Kromatogram Cair Ekstrak
LAMPIRAN 5
65
Gambar 18. Hasil alignment sekuens hasil sekuensing dengan sekuens Gymananthemum
amygdalinum yang diperoleh dari NCBI Genbank
LAMPIRAN 6
Gambar 19. Kromatogram Sekuens DNA menurut Primer ITS 1
66
LAMPIRAN 7
Gambar
Sekuens
ar 20. Kromatogram S
ek
kuens DNA menurut Primer ITS 4
67
LAMPIRAN 8
68
69
70
Gamb
mbar
ar 221.
1. B
LAST
LA
ST Sekuens
Sek
kuenss D
NA Nan
Nan Fei
Fe Shu
Gambar
BLAST
DNA
LAMPIRAN 9
Sekuens Gymnanthemum amygdalinum yang diperoleh dari NCBI GenBank
(fasta format)
>gi|40748098|gb|AY504695.1| Gymnanthemum amygdalinum internal transcribed
spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete
sequence
TCGATTAAAGCATTTCAGAATAACCTGTGAACATGTATTATTAT
TTGGGTGTTGGGAGGACGGGCTAATGCTCTCACCCTCTTTGCAT
CGTGTTGACATACACTTGTATAGCCTCTTWTTGGGTCGTACATG
TGTCTTGTTAGCATTTAAACAAACCCCCGGCACAGAACGTGCCA
C
C
GGG GCG C G
AGGATGAACAAAACATTAAAAGGGTGCGACTTGTGATGCCCCG
TATGCATTC
CAG
AGGTCGTG
GGC
G TTTTTTGT
TTCGCGGTATGCATTCAGGTCGTGGCTTTTTTGTAATTACAAAC
GGCAACG
CGG
GATA
ATCTCGGCT
TCA
CACGCAT
GACTCTCGGCAACGGATATCTCGGCTCACGCATCGATGAAGAA
AAAATG
TGCGAT
ATAC
ACTT
TTGG
GGTG
TGTG
T AA
ATT
T GCA
CGTAGCAAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGA
AGT
TTT
TTTT
TTGA
GAAC
ACG
GCAA
AGT
GTTG
T CG
CGCC
CCCG
C A
ACCATCGAGTTTTTGAACGCAAGTTGCGCCCGAAGCCATTTGGT
CACG
CGTC
TCTG
TGC
CCTGGGCGTCAC
CGC
GCAT
ATTG
TGC
CGAGGGCACGTCTGCCTGGGCGTCACGCATTGCATCGCCTCCTT
TCC
CCTT
TTC
CCTTAGTAGGCTTGTTGT
GTTC
TCGG
GG
CAATGCCTCCTTCCTTAGTAGGCTTGTTGTTCGGGGCGGAGATT
CAT
ATGC
GCTGATGGTGTGGTTGGCCT
CTAA
AAA
A
GGTCTCCCATGCTGATGGTGTGGTTGGCCTAAACGTAACTCCCT
GATA
ACATGACTAGTGGTGGTTGA
ACA
AA
TTCGGTGGATACATGACTAGTGGTGGTTGACAAGACCTTCGTTT
TGT
GTG
GTCGTTAACCGTAAGGGAAA
AAGG
GGT
T
GGAGTTGTGTGTCGTTAACCGTAAGGGAAAGGTTGTAAAAATC
GAGTC
CGT
TCT
CTTA
TATG
TGAT
ATGA
GACG
CGCT
CTTC
CGA
A
CCTTAATGAGTCGTCTTATGATGACGCTTCGA
71