Upaya memacu pertumbuhan lean domba pedaging dengan modulasi testosteron endogen menggunakan ekstrak pasak bumi (Eurycoma longifolia Jack)

 
 

UPAYA MEMACU PERTUMBUHAN LEAN DOMBA PEDAGING
DENGAN MODULASI TESTOSTERON ENDOGEN
MENGGUNAKAN EKSTRAK PASAK BUMI
(Eurycoma longifolia JACK)

ZUBIR

SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2012

 
 

 
 

PERNYATAAAN MENGENAI DISERTASI DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa disertasi Upaya Memacu Pertumbuhan
Lean Domba Pedaging dengan Modulasi Testosteron Endogen Menggunakan
Ekstrak Pasak Bumi (Eurycoma longifolia Jack) adalah karya saya sendiri dengan
arahan dari komisi pembimbing dan belum diajukan dalam bentuk apa pun kepada
perguruan tinggi mana pun. Sumber informasi yang berasal atau kutipan dari
karya yang telah diterbitkan maupun tidak diterbitkan dari penulis lain telah
disebutkan dalam teks dan dicantumkan dalam Daftar Pustaka di bagian akhir
disertasi ini.

 
 

 
 

ABSTRACT
ZUBIR. Lean Growth Promotion of Sheep by Endogenous Testosterone
Modulation with Eurycoma Longifolia Jack Extract. Under direction of RUDY

PRIYANTO, EDDIE GURNADI, and WASMEN MANALU.
Pasak bumi (Eurycoma longifolia Jack/ELJ) is frequently claimed to
increase the expression of masculinity, including induced the lean growth.
Bioactive components that play a role in this case was identified as a peptide
compounds. The ELJ peptides potentially become a natural ingredients to improve
carcass production and quality. The experiment was aim to obtain and
characterization peptide extract from root of ELJ, to assess the ability of some
protection methods of the peptide in the rumen and to observe the influence of
ELJ peptide supplementation on growth, carcass and meat characteristic of rams.
The experiment was conducted in three phases. The first phase was extraction and
characterization of peptides from ELJ root. Extraction was based on the solubility
properties of peptides in the acid-base solution. Characterization of the extract
included proximate analysis, electrophoresis and GC-MS. The second phase
studied ELJ peptide protection in the in vitro rumen with three methods. These
methods were polymeric encapsulation with a primary coat are 2-vinylpyridine+
styrene, hydrophobic encapsulation with stearic acid+beeswax and binding
peptide by tannic acid. The third phase was applied to sheep meat. Sixteen rams
were divided into 4 treatment groups in the form of ELJ extract suplemented 0,
1.5, 3 mg/kg BW for P0, P1, P2 respectively and LJ100™ 1 mg/kg BW for P3.
Observations were made on growth, feed efficiency, body composition, carcass

and meat traits. The yield of the extraction from the ELJ root was 0.53% in dry
matter with a peptide content of 34.57% and peptide recovery was as much as
8.30%. The resulting peptides have a wide range of bands, but all have a
molecular weight below 45 kD. Hydrophobic encapsulation gave the most
protective peptide based on organic matter digestibility and NH3 production.
There were no significantly differences between treatments on ram growth
performance, carcass yields and plasma testosterone level. However, the treatment
affected significantly (P