Detection of IgM antibody against phase 1 flagelin for diagnosis of Salmonella paratyphi A infection

Suppl

Poster

I - 1998

Session

Detection of IgM antibody against phase 1 flagelin
for diagnosis of sslmonella paratyphi A infection
S. Korbsrisate, S. Sarasombath, P' Ekpo, N' Praaporn,

273

P.L4

M' Hossain' S' McKay

Abstrak
Salmonella ParatYPhi A
kurang memuaskan. Untuk m

nukleotida 460 - 890, P S K- 8E'

sejak timbulnya clemam. Serum yang tlidapat lebih dini atau
ènelitian ini menunjukkan uji yang dikembangkatt untuk deteksi

l2

serum pasien diambiL pada saat yang tepat'
antibodi IgM ini dapat digunaka,x untuk diagnosis infeksi S. paratyphi A apabila

Abstract
ic teclniques availcurrent
Salmonella paratyphi A is a pathogenic bacteria that causes
positiorts 460-890'
I
Jtage
able are unsatisfactory. To improve tliagnosis, plasmid encodirtg
enzyme-linked
irect
use

een
prote
pSK-88, has been corzstructetl' Tlrc recombinatxt
S' ty
A,
p
hemoculture
with
sera
detection.
antibody
immunosorbent assayfor IgM
ed' The
the healthy control
ltositive antl granr-negativi bacteria,,dengue hemorrhagic fever
92'l7a'
56.8Vo,
ctre
predi
t'alues

nostic sensitivity, speciJicity, fficacy, poritive anel negative
patients antL the IgM Levels are analysetl'
the
samples
serum
the
taking
time
th.e
from
about
data
therefore,
sensitivity is not satisfactory,
obtained 9-12 days after onset offct'er
It hns been shown that seniiririry oy rh" test can be increased up to 83.37o if the serwn have been
This result suggests that the tleveloped
respectiveLy.
sensitivity,
66.6va

and
The serum obtained earlier or later ( t4days) gave ot.rly 34.8va
samples have been taken at the apserum
the
when
A
infection
paratyphi
o/
S.
ctiagnoiis
powerfiil
IgM antibody detection assay is
for
propriate time.

Department of Imtnunology, Faculty of Medicine,
Siriraj Hospital, Bangkok 10700, Thailand.