New simple method for detection IgM antibodies against Salmonella typhi infection on human
208
Typhoid Fever and other Salmonellosis
New simple method for detection IgM antibodies against
Salmonella typhi infection on human
Med J Intlones
D2-2
M. Hattal, L. Chairudin Ll, Henk L. Smits2
Abstrak
prinsip meto.la yang digunakan adalah penggunaan dipstick. Dipstick mengandung 2 pita horizontal; pita antiSen mengandung
antigen S. typhi (pita yang ii bawahl dan pita kontrol (di atas). Dasar tlari tes ini ialal't pengikatan antibotli IgM manusia spesifik
diLakukan dengan
SalmoneLla dengan antigei spesifik Salmonella. IgM yang terikat tlidereksi dengan konjugat anti hunnn IgM. Tes ini
suclah clibasahi diinkubasi pada larutan tersebut. Timbulnya wama patla
pada sampel serum. Peran pewarnaan ini penling pada interpre'
Salmonella
spe;ifik
antigen memperlihatknn atlanya antibotli IgM
pembancling
intensitas warna. Sebagai kesinrpulan' metocla bant
sebagai
cligunakan
untuk
warna
referensi
Tersedia
strip
tasi hasil tes.
tlan seclerhana lni (dipstick) mungkin bermanfaat unîuk ntendeteksi IgM terhadap infeksi S. lypht patla mbnusia tanpa metnbutuhknn
alat khttsus dan ketrampilan clan dapat diterapkan pada perawatan kesehatan masyarakat di tingkat pedesaan'
pengenceran serttm I :50 clal.am-reagen deteksi, dipstick yang
'piti
Abstract
consisting
of
The principle of this methotl was using the clipstick. The dipstick contains two horizontal bands; an antiSen band
of human salmoantigen prepared from S. typhi (lower band) ancl an internal control (upper band). The assay is based on the binding
an
anti-hwnan IgM
with
detecîed
specificalLy
are
antibodies
IgM
Bound
antigen.
specific
to
the
Salmonella
IgM
antibo4ie.s
neilà specffic
wettecl
disptick in rhis
a
incubating
dye co,njugate. The assay is performeil by nnking a I :50 dilution of sera in the detection reagent and
The
strenght of
sample
in
the
serwn
IgM
antibodies
presence
of
salmonella-specific
solution. Staining oy the aniigin band reveals the
the
staining
to
conlpare
is
usecl
reference
strip
provitlecl,
coloured
The
resl,lt.
test
of
the
the
interpretation
tlrc staining is important in
infectiott on humart
inrensity. In,on irsion, this new simpLe methotl (ttipstick) may benefitfor derect the IgM antibodies against S. typhi
without needetl special equipment and skill and can be applied in public heabh care in vilLage level.
1
2
Microbiology Dept, IJNHAS, Ujung Pandang, Indonesia
RoyalTropical Institute, The Netherlands
Typhoid Fever and other Salmonellosis
New simple method for detection IgM antibodies against
Salmonella typhi infection on human
Med J Intlones
D2-2
M. Hattal, L. Chairudin Ll, Henk L. Smits2
Abstrak
prinsip meto.la yang digunakan adalah penggunaan dipstick. Dipstick mengandung 2 pita horizontal; pita antiSen mengandung
antigen S. typhi (pita yang ii bawahl dan pita kontrol (di atas). Dasar tlari tes ini ialal't pengikatan antibotli IgM manusia spesifik
diLakukan dengan
SalmoneLla dengan antigei spesifik Salmonella. IgM yang terikat tlidereksi dengan konjugat anti hunnn IgM. Tes ini
suclah clibasahi diinkubasi pada larutan tersebut. Timbulnya wama patla
pada sampel serum. Peran pewarnaan ini penling pada interpre'
Salmonella
spe;ifik
antigen memperlihatknn atlanya antibotli IgM
pembancling
intensitas warna. Sebagai kesinrpulan' metocla bant
sebagai
cligunakan
untuk
warna
referensi
Tersedia
strip
tasi hasil tes.
tlan seclerhana lni (dipstick) mungkin bermanfaat unîuk ntendeteksi IgM terhadap infeksi S. lypht patla mbnusia tanpa metnbutuhknn
alat khttsus dan ketrampilan clan dapat diterapkan pada perawatan kesehatan masyarakat di tingkat pedesaan'
pengenceran serttm I :50 clal.am-reagen deteksi, dipstick yang
'piti
Abstract
consisting
of
The principle of this methotl was using the clipstick. The dipstick contains two horizontal bands; an antiSen band
of human salmoantigen prepared from S. typhi (lower band) ancl an internal control (upper band). The assay is based on the binding
an
anti-hwnan IgM
with
detecîed
specificalLy
are
antibodies
IgM
Bound
antigen.
specific
to
the
Salmonella
IgM
antibo4ie.s
neilà specffic
wettecl
disptick in rhis
a
incubating
dye co,njugate. The assay is performeil by nnking a I :50 dilution of sera in the detection reagent and
The
strenght of
sample
in
the
serwn
IgM
antibodies
presence
of
salmonella-specific
solution. Staining oy the aniigin band reveals the
the
staining
to
conlpare
is
usecl
reference
strip
provitlecl,
coloured
The
resl,lt.
test
of
the
the
interpretation
tlrc staining is important in
infectiott on humart
inrensity. In,on irsion, this new simpLe methotl (ttipstick) may benefitfor derect the IgM antibodies against S. typhi
without needetl special equipment and skill and can be applied in public heabh care in vilLage level.
1
2
Microbiology Dept, IJNHAS, Ujung Pandang, Indonesia
RoyalTropical Institute, The Netherlands