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Pengujian Keutuhan Membran Plasma Spermatozoa Semen Cair Domba Menggunakan Larutan Hipoosmotik

Prosiding Seminar Hasil-Hasil Penelitian Bidang llmu Hayat

PENGUJIAN KEUTUWAN MEMBRAN PLASMA SPERMATOZOA SEMEN
CAIR DOMBA MENGGUMAKAN LARUTAN NIPOOSMOTIK
Arifiantini, R.I. I), B. ~urwantara"dan W.W. putra2)
" Bagian

Reproduksi dan Kebidanan Fakultas Kedokteran Hewan IPB
2' Mahasiswa Fakultas Kedokteran Wewan IPB

ABSTRACT
An experiment was conducted to study the integrity of ram sperm plasma membrane using
hypoosmotic solution as part of semen quality evaluation. Semen from six rams were
collected using artificial vagina and evalusted macro- and nricroscopically. The collected
semen were diluted in egg yolk-citrate extender and stored in refrigerator for 7 consecutive
days. The preserved semen were examined to evaluate the motility and its plasma membrane
integrity after exposed to incubation temperature for 15, 30, and 45 minutes. The result of
the experiment showed a significant correlation between motility and sperm plasma
membran integrity. Correlation coefficient between motility and sperm plasma membrane
integrity for 15, 30, and 45 minutes incubation time were 0.96, 0.97 and 0.98 respectivelly,
(P

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