Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Keynote Invited Papers Page 25
To obtain an optimum condition on formaldehyde sensor, the pH of buffer solution is varied at pH 1.0
– 8.0. The calibration curve for formaldehyde standard solution at different pH is presented in Figure 7. It is seen that the buffer condition influenced the sensor sensitivity,
where the optimum condition is obtained at pH 3.0. The sensor give a relative high sensitivity at low pH pH 1.0-5.0 and consistently decrease the sensitivity at high pH condition pH 6.0
– 8.0. However, the detection linearity is not influenced by the pH condition. Therefore, all measurement is carried out at pH 3.0.
13. Selectivity of Formaldehyde Sensor
The selectivity of formaldehyde sensor has been examine for formaldehyde in the absence and in the presence of suspected interfering agents. Interferents 2.0 ppm, which are commonly
found in food samples are added in to 2.0 ppm formaldehyde standard solution. The single and mixture analytes are determined by using formaldehyde sensor, and the response
sensitivity is summarised in Figure 8. The results showed that every single interfering agents give small response in UV-vis spectrophotometer. At high concentration of interfereing
agents are also reduce 4-5 the responses formaldehyde standard solution. However, the sensor is free from interferent when the concentration of interfering agents are low 2 ppm.
Ascorbic acid is the most potential interfering agent.
Figur 8. Response selectivity of a sensor into formaldehyde and interference. Single interfering agents 5.0 ppm and their mixture with 2.0 ppm formaldehyde standard solution is analysed in UV-vis
spectrophotometer at λ 560 nm. Experimental parameter is the same as in Figure 6.
14. Conclusion
The electrodeposited polymer has been demonstrated to be compatible in the production of reprooducible sensor. The application of electrodeposited polymer film as a very good matrix
polymer for immobilization of active materials which are suited for the construction of biosensor and chemical sensors. An uric acid biosensor in electrochemical detection system is
the example of reproducible biosensor which is developed by using deposited polytyramine. The uric acid biosensor showed a sensitive response to uric acid with a linear calibration
curve lies in the concentration range of 0.1
– 2.5 mM, slope 0.066 µAmM, and the limit detection was 0.05 mM uric acid SN=3. Another example of sensor is formaldehyde sensor
that is developed by immobilization of chromatophic acid onto a conducting transparent plastic and interfaced in UV-Vis spectrophotometer detection system. The linearity of
formaldehyde sensor lies in the range concentration of 0.1-4.0 ppm formaldehyde, where the detection limit is 0.05 ppm formaldehyde.
0,2 0,4
0,6 0,8
1
A b
so rb
a n
ce a
u
Formaldehyde 2 ppm and Interfering agents 5 ppm
Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Keynote Invited Papers Page 26
Acknowledgement
The research grand from Project KOMPETENSI DP2M Kemenristek Dikti Indonesia for funding aspects of this work is gratefully acknowledged.
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Hall, E.A.H., 1991,
Biosensor,
Prentice Hall. Englewood Cliffs, New Jersey. [3].
Trougakos, I.P., 2013, The Molecular Chaperone Apolipoprotein JClusterin as a Sensor of Oxidative Stress: Implications in Therapeutic Approaches
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