Arcangelisia Flava Increases Rats' Leukocytes but has Biphasic Effect on Rats' Lymphocyte
.f.{s%s
K#pffiffi rRffis@
s"€s
Drug Deiivery Systems:
From Drug-Discovery, Pre-formulaticn, Formulation and Technological Approaches for
Poorly Soluble Drugs and Protein
..',...-.-.,
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The l"t lnternational conference on Pharmaceutics
& Pharmaceutical Sciences Proceedings
ISBN : 97 8-5A2-72333-0-0
Date 18 March 2015)
(Letter of National ISBN Agency No' 4L27lE'slp/03'2015
1st edition Proceeding
Published bY:
Faculty of Pharmacy Universitas Airlangga
Surabaya, lndonesia
Address:
Kampus B Jl. Dharmawangsa Dalam
Surabaya 60286
Phone +62315033710
Fax +62 31 5020514
Website : www.icpps2014.com or wwwff'u na i r'ac'id
Email: [email protected]
rsBN 1?A-t0e-?e333-0-tl
.IIil!Jil[UruUuuililil
PREFACE
From Chairman
It is our pleasure to
present you the proceedings of The Lst lnternational Conference on
Pharmaceutics and Pharmaceutical Sciences (ICPPS) organized by The Faculty of Pharmacy
Universitas Airlangga Surabaya lndonesia.
The proceeding was produced based on papers and posters presented at The
1st
lnternational
Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS), held in Surabaya, lndonesia, 14-
15November2014.
The proceeding clearly reflects broad interest, from the participants that coming from all around the
world.
The papeJ$presented were pharmaceutics and biopharmaceutics; requirements on howto evaluate
molecufp! in discovery and their appropriateness for selection as potential candidate; their
development in context of challenges and benefits, together with associated time and cost
implications and also requirements to progress through pre-clinical and clinical.
ln this an opportunity, lwould like to express my appreciation to the editorialteam of the proceeding
who have been working hard to review manuscripts, and making the first edition of this proceeding
be possible.
I would like also to thanks to all invited speakers and presenters who participated in The 1st
lnternational Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS) and your
contribution to this proceeding.
Finally, I hope this proceeding will give contribution
Sciences research.
Chairman,
Dra. Esti Hendradi, MSl., Ph.D., Apt
to the Pharmaceutics and Pharmaceutical
COMMITTEE
Advisor
Chairman
Editor
Universitas
: Dean of Faculty of Pharmacy
Ph'D' APt'
: Dra. Esti Hendradi, M'Si"
Airlangga
(Netherlands)
: Dr. Wouter U. Hinrichs
(JaPan)
Prof. Kozo TakaYama
Mutalik (lndia)
or. ttuttakorn Baisaeng {Thailand)
(lndonesia)
Prof. Dr. WidiiSoeratri' DEA
Dr. Srinivas
n
,rtn
Committee:
APt'
Prof. Dr. Widji Soeratri, DEA'
APt'
Prof. Dr. Siswandono, MS''
APt'
Prof .Dr. Tutuk Budiati' MS''
Yuwono' MS'' Apt'
Prof. Dr. rer'nat' Muhammad
APt'
Drs. Hadi Poerwono, MSc''Ph'D'
MSi'' Ph'D' Apt'
Drs. Marcellino Rudyanto'
APt'
Drs. Bambang Widjaja, M'Si'
Drs. SugiYartono, MS, APt'
APt'
Dra.Tutiek Purwanti, MSi'
APt'
Dra.Tristiana Erawati, MSi'
Dra.Retno Sari, MSc, APt'
Dra.Dewi lsadiartuti, MSi' APt'
Dra.Noorma Rosita, MSi' APt'
APt'
Dr. DwiSetyawan, SSi, MSi"
Primaharinastiti' SSi' MSi' Apt'
M'Phil'' Ph'D' Apt'
Dewi MelaniHariyadi' SSi'
APt'
HelmY Yusuf, SSi, MSc'' Ph'D'
AptDwi Lestari' SS|'MPharmSci''
Rrdhani
Dr. rer. nat. wtaria l-ucia
M'Sc' Apt'
tvt"lanny lka Sulistyowati' SFarm'
Apt' - '
Febri Annuryanti, SFarm'M'Sc'
M. Faris Adrianto, MFarm' APt'
Dini Retnowati, SFarm,APt'
Abhimata P, SFarm, APt'
Or.
ni"tt.
TABLE ofCONTENT
Preface from Chairman
Committee
Table of Contents
Author
...... ii
....--..--..--.
................. iii
lndex
.................... iii
AUTHQR INDEX
COMPARISON OF SODIUM STARCH GLYCOLATE AND CROSSCARMELLOSE SODIUM AS
SUPERDISINTEGRANT IN MEFENAMIC ACID FAST DISINTEGRATING TABLET
Adeltrudis Adelsa D, Oktavia Eka Puspita, Amalia Ayuningtyas, Marulita lsadora ,..........,........ 1
STUDY EXPRESSION OF HUMAN ERYTHROPOIETIN EXPRESSSION IN MAMMALIAN CELL
Adi Santoso, Popi Hadiwisnuwardhani, Yana Rubiana, Yulaika Romadhani, Endah Puji
Septisetyani, Dyaningtyas D.P. Putri
ANTIOXIDANT STABILITY ASSAY OF ALPHA TOCOPHERYL ACETATE IN SOL]D LIPlD NANOPARTICLE
SYSTEM (LlPlD BASE BEESWAX AND MONOSTEARIC GLISERYL
50:50)
Anggie Widhi, Noorma Rosita, Widji Soeratri ......................
A BIOACTIVE BOVINE HYDROXYAPATITE4ELATIN IMPLANT FOR IN VITRO GENTAMIC]N RELEASE
..... 13
Aniek Setiya Budiatin, M. Zainuddin, Junaidi Khotib, Diah Himawati
EFFECT OF COMPARISON SURFACTANTAND COSURFACTANT IN WATER/OIL MICROEMULSION IN
OVALBUMIN Microemulsion Water/Oilwith Surfactant (Span 8O-Tween 80) :
C-osurfactant (Ethanol) =5:L,6:!, and 7:1)
................. 18
Anisa Rizki Amalia, Riesta Primaharinastiti, Esti Hendradi ........,......,
RELEASE OF
ANALYSIS OF MYCOLIC ACIDS CLEAVAGE PRODUCT OF
Mycobacterium tuberculosis
CHROMATOGRAPHY-FLAM E IONIZATION DETECTOR
Asri Darmawati, Deby Kusumaningrum, lsnaeni, Muhamad
Zainuddin
PERIPLASMIC EXPRESSION OF GENE ENCODING ANTI-EGFRvIIISINGLE-CHAIN
ANTIBODY USING PelB LEADER SEQUENCE lN ESCHER/CHIA COLI
BY GAS
.............. 21
VARIABLE FRAGMENT
lGrtika Sari Dewi, Debbie Sofie Retnoningrum, Catur Riani, Asrul Muhamad Fuad ................24
IN VIVO ANTIMALARIAL ACTIVIry OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION OF
Alectryon serrotus LEAVES ON Plasmodium bergheilNFECTED MICE
Aty Widyawaruyanti, Uswatun Khasanah, Lidya Tumewu, Hilkatul llmi, Achmad Fuad Hafid,
Indah S Tantular ...............
..................... 30
PROFILE OF COMMUNIry PHARMACISTS KNOWLEDGE IN PATIENT ASSESSMENT WITH INFLUENZA
SYMPTOMS AND ITS PRODUCTS
Azza Faturrohmah, Arie Sulistyarini, Ana Yuda
...........
..........,........ 33
SOLUBILIry AND D]S5OLUTION STUDY OF KETOPROFEN
INCLUSION COMPLEX (Prepared by Kneading Method)
BambangWidiaia, Achmad Radiaram, Arafah Zulhana
-
HIDROXYPROPYL'P-CYCLODEXTRIN
"""""""""
"""""':""""
37
FoRMUIATIoNANDSTABILITYTESTINGoFMELoXICAMSoLIDDISPERSIoNGEL
BudipratiwiWisudyaningsih,lnkaDewiNurAnggaraini,FersiyaWardani.......'..40
EFFECT OF
DICLOFENAC SODIUM MEMBRANE-TYPED
MENTHOLAS PENETRATION ENHANCER TO
TRANSDERMAL PATCH
CHARACTERIZATION
Khotib
^a
"""" rJ
Destria lndah Sari, Esti Hendradi, Junaidi
&
PHYSICALCHARACTERISTICSANDRELEASESTUDYoFoVALBUMINFRoMALGINATEMICRoSPHERES
UsING AERoSoLlzATloN
coNcENTRATIoN oF ALGINATE AND BaCl,
PREPARED BY DIFFERENT
TECHNIQUE
-!-:r:- .--^.r.-L.,..*inah^c
Ermawahyuningtyas
Dewi Melani Hariyadi, Tristiana Erawati, sisilia
------------..-......... 40
OF SAMBILOTO (Andrographis paniculata)
MUCOADHESIVE TABLET OF ETHANOLIC EXTRACT
AS ANTIDIABETIC USTNG CHITOSAN
DhadhangWahyuKurniawan,HeningPratiwi,andlingga|kaditya....................50
PHYSICALINTERAqtIoNsTUDYoFIBUPRoFEN-STEARICAcIDBINARYMIXTURE
Diaieng Putri Paramita, Dwi Setyawan, Dewi lsadiartuti
.
OF L-(3,4-Dichlorobenzoyl)-l',3-dimethylurea
MOLECULAR MODELTNG AND SYNTHES]S
' ;;"';;;
r""-t-"riur*"
Siswandono, BambangTri
Purwanto
59
""""""""""'
"""""""'
""""""""""""
53
EXPRESSIoNoFRECoMBINANTHUMANGRANULocYTE-coLoNYSTIMULATINGFAcToRWITHIN
COIi USING PEIB LEADER PEPTIDE
PERIPLASMIC COMPARTMENT OF FSChETiChiO
Dian Fitria Agustiyanti, Asrul Muhamad Fuad
(EICUIhET|NC
FROM BULBS OF BAWANG TIWAT
EVALUATION OF ANTIHYPERURICEMIC ACTIVITY
patmifolia (1.) Merr.) BY lN VITRO AND lN VIVO STUDIES
Astika
Dian Ratih Laksmitawati, Rininta Firdaus, Yulinda, Mediana
""""'
"""""72
OF COMBINATION OF STRAWBERRY FRUIT
ANTIOXIDANT ACTIVITY OF Xil%ETHANOL EKTRACT
L') uslNG ABTS FREE RADICAL
(Fragoria x anan(rssa Duch.) AND STARFRU 11 (Averrhaa carambola
SCAVENGING
METHOD
Diana Serlahwaty, lndira Natalia
"""""""""' -?
'o
Timang
ATORVASTATIN BY MICROCRYSTALLIZATION
ENHANCEMENT OF SOLUBILITY AND DISSOLUTION
METHOD
Dolih Gozali, Yoga Windu Wardhana, Ronny
Tandela
""""""""""' rz
INVITR}ANTIMALARIALACTIVITYoFDIcHLoRoMETHANESUB.FRACTIoNoFEuco/yptusglobulus
L. Stem AGAINST Plasmodium folciporum
""""""""' 85
Elis Suwarni, Achmad Fuad Hafid, Aty Widyawaruyanti
ATcangelisiaflovaINCREASESRATS.LEUKoCYTESBUTHASBIPHASICEFFECToNRATS'LYMPHoCYTE
' ina"f puspiiasari,
Evi Umayah Ulfa,
vita Ariati, Mohammad sulthonHabibi
""""""""""""
89
Conierence
& Pharmaceutical Sciences
Arcangelisia flava INCREASES RATS, LEUKOCYTES
BUT HAS BIPHASIC EFFECT ON RATS' LYMPHOCYTE
tL e_
3rTri
us.
@
rcPtfuol4
lLil*
frsari.
Faculty of Pharmacy University of Jember; Jl. Kalimantan 37 Jember, lndonesia, e.puspitasari@
Frf l'.tmavah Ulfa, Faculty of Pharmacy University of Jember, il. Kalimantan 37 jember, lndonesia;_@
ulillrr c: Pharmacy University of Jember, Jl. Kalimantan 37 Jember, lndonesia;
Mohammad Sulthon Habibi.
4ar,nary University of Jember, Jl. Kalimantan 37 Jember, lndonesia.
gJ\ e
si
€?tli-S
altlr
Linerg5.
992
le
to4am
rd
untl
rich of natural resources, esperrdicinal plant, lndonesia has many
to
.rr oe fr
ll6r l
)€ h{illl
rtia C
Medamrc-
32'
Fdu-aqr
Reorn:
tK6
epCI1
develop medicinal sources
fiom plants. One of the potential
:'lant used for cancer chemopreagpnt is Arcangelisia flava. This plant
to have antioxidant and cytotoxic
aganst breast cancer cell line, MCFwere considered to be corre=nr[ity
:D the alkaloid content, especially
Keawpradub et al., 2005). As this
E
=ry to be found in Meru Betiri Na-
re
;itr.rated in Jember (Koran Jakarta,
are eager to explore further about
mreloped as medicine, we have to
;afety. The sub-chronic toxicity assay
to study the safety of ethanolic
nf 4- flava leaves (EEAfL) use. This re,mms Cone to determine the EEAfL
effect
,les dnd lymphocytes cell count of
sub chronic EEAfL.
;ll was done to determine the EE:'r rmmune response, especially on
;rd lymphocytes cell count of rats
,;l
chronic EEAfL.
AND METHODS
.=1s and extraction
frmr: ieaves were collected from Meru
Park, Jember, lndonesia. They
le::ed for their freshness, old age,
r on€s. The leaves were washed
M::nal
I
n
thoroughly with water, then, were air dried
followed by oven drying at 50 0C" The dried
leaves were grounded and sieved. The etha-
nolic extract were prepared using 500 g of
leaves powder according to the previous
study with a slight modification (Keawpradub
et al., 2005). The ground-dried leaves was
sequentially extracted with n-hexane, chloroform, and ethanol. The extraction was repeated three times for each solvent. The ethanol
extract was evaporated under reduced pressure (Heidolph, Laborota) resulting EEAfL. EEAfL was then suspensed in CMC Na 1% before
being administered to the animal.
Animals
Male Wistar rats (weighing 100-150 g) were
housed at a constant temperature and lightdark cycle. Rats were fed with standard feed
and water as libitum. The rats were acclima-
tized and quarantined for at least 10 days
prior to the experiment. The animal handling
protocols of this study were in accordance
with the guidelines of the animal care of University of Jember.
Experimental design
Fifteen rats were devided into three groups.
Group I as control, received CMC Na 1 %.
Group ll received EEAfL 500 mC/kC BW Group
lll received EEAfL 1,500 mglkg BW The treatment was done orally for 11 days. At the 12th
day, the blood sample was collected and analyzed further for leukocytes and lymphocytes
cell count.
Statistical analysis
All data were presented as mean + the stan-
koceeding
@rcpfuor4
nrelofrternational
on Pharmaceutics & pharmar
dard error of the mean (SEM). Kruskal
Wallis
assay followed by Mann Whitney
test were
used
Discussion
to increase the
of normal rats, as it was
cytes cell count. But it had
lymphocytes, as the higher the
EEAfL seems
to know the significance difference be_
tween groups, since the data could
not meet
the requirement for Anova analysis (p <
0,05).
the lymphocytes cell count. We
high dose of EEAfL decreased
system as it decreased the
AND DISCUSSION
Leukocytes cell count
The leukocytes cell count (Figure
1) of rats re_
ceiving EEAfL were increased significantly
than
that ofthe control group, either at the
dose of
500 or 1,500 mglkg BW
Lymphocytes cell count
The lymphocytes cell count of rats
receiving
RESULTS
count.
EEAfL contained berberine (pusp
Ulfa, 2013). The higher the dose,
the berberine content. Berberire
rmmunosuprressive agent. lt
tivation and proliferation of T
cytotoxic effect known (Xu et aL I
AfL might increase the non-spesft
system. Leukocytes consist of
500 mg/kg BW EEAfL was increasing
signifi_
cantly, but the rats treated with EEAiL
ai tn"
dose of 1,500 mg/kg BW had U".r""r"J
tyr_
phocytes (Figure 2).
lymphocytes, monocytes,
basophyls. Lymphocytes plays rob
rmmune response, while others d
specific immune response.
Leukocytes cells count that was
while the lymphocytes cell count
creasing after treated with high
-2(m
g 1ym
Er(}m
-
rgrorp
I
f6roup
I
qgrsup ilt
5m0
suggested that the other kinds
0
involving
2mm
CONCLUSION
1Sm
the results, we can
EEAfL increased the immune
rats, but it had biphasic effect
on
suggesring that high dose of EEAfl_
creased non-specific immune
Based on
1S0O
5 1SS
: lDS
g
1lxf,F
48ffi
€ oinc
E
i 400
lffu
agrolp
I
ilgralp
tr
stead
o
Figure 2. LymphocyJ*,, count
of rats. Data
represented as mean + SEM (n
= 5). Different
letter n otatio n exp ressed, ign in.a
ni O iff*r"
according to Mann Whitney test (p
< 0,05).
'*,'
i
u,* *
d
non-specific immurp
were higher. Water extract of A.
macrophage activity (Florentina,
EEAfL might also increase the m;
tivity. Still, we need to examined
these hypothesis.
Figure 1. Leukocytes cell count
of rats. Data
represented as mean + SEM (n
= 5). Different
letter n otatio n exp ressed s ign ifi
ca ni d iff" r" n."
according to Mann Whitney test (p
< 0,05).
:.
in
-
'-
]
*-".'
33"t'." o''."rou, :'; ,r-f .r,u**
ofthe specific one.
REFERENCES
*u
1..
Florentina, D. (2013). pengaruh
Larut Air Kayu Kuning (Arcangelisiel
(1.) Merr.) terhadap Fagositosis
tt*
rofag secara ln Vitro pada Tikus
l
s-"r,::i:;,
'tL$,:it1--;1i:#
$;r_li{i:'rli1i+tr*.1r!
l'.1*,:jil9+!,, i'
.i.,:..:iii:, :.,:1ii::r1..,
.
r
: F,E::+;:,,
hoceeding
tblo
herndionat
qr
I
Gonf eneme
Pharmaceutics & Pharmaceutical Sciences
6
L
Universitas 4.
Wistar. Yogyakarta: Skripsi
Gadjah Mada. Hal.
Keawpradub, N., Dej-adisai, A., and
nyongsawad, S. (2005).
Cytotoxic Activities of Thai
Puspitasari, E. and Ulfa, E.U' (20L3).
Pengembangan Ekstrak Etanol Ar
cangelisia flava Terstandar sebagai Agen
Pendamping Kemoterapi Doxorubicin
untuk Pengobatan Kanke; Laporan Ta
NartsNamedKhaminkhruea:Arcangeli
hunanPenelitian,LembagaPenelitian
39.
Yue
Antioxidant
Medicinal
rd
fr,arra,
Coscinium blumeanum
illUzrea tinctoria.The
and
Songklanakarin 5.
t6
Jhurqd of Science and Technology.2T(2\.
saftr
f,uran Jakarta (2}1I2l. Peneliti
@27-
e
&,ntikanker
€r
s
rhc
ffi[
E
fie'
tln
,laY
b
hat
rse
cf
f
le
nEl
20131.-
d fu!+5ir
onclttde
respcne
Aft
Temukan
di Meru Betiri, Koran Ja
fngital Edition, http://kora n-jakar
tse
m-gfn
resporse
aruhr
Ekfd
lngelliia
tosis
h|l"ar
Tikus Gatur
@
rcP#p2o*4
.phpldetail/viewO1/
diakes 14 Maret 2013
Universitas Jember'
Xu, L', Liu, Y., and He, X. (2005)' lnhibi
tory Effects of Berberine on The Action
and Cell Cycle Progression of Human Pe
ripheral Lymphocytes' Celluler and Mo
lecular lmmunology- 2(41' 295-3OO'
K#pffiffi rRffis@
s"€s
Drug Deiivery Systems:
From Drug-Discovery, Pre-formulaticn, Formulation and Technological Approaches for
Poorly Soluble Drugs and Protein
..',...-.-.,
'.t
I
.4. :lt-
D
::
-.....
.;
.:
..'l}.:'1;:r'x.]q{':
:l-
!
.'{:9'
i::r'r,:tr*
',t:
The l"t lnternational conference on Pharmaceutics
& Pharmaceutical Sciences Proceedings
ISBN : 97 8-5A2-72333-0-0
Date 18 March 2015)
(Letter of National ISBN Agency No' 4L27lE'slp/03'2015
1st edition Proceeding
Published bY:
Faculty of Pharmacy Universitas Airlangga
Surabaya, lndonesia
Address:
Kampus B Jl. Dharmawangsa Dalam
Surabaya 60286
Phone +62315033710
Fax +62 31 5020514
Website : www.icpps2014.com or wwwff'u na i r'ac'id
Email: [email protected]
rsBN 1?A-t0e-?e333-0-tl
.IIil!Jil[UruUuuililil
PREFACE
From Chairman
It is our pleasure to
present you the proceedings of The Lst lnternational Conference on
Pharmaceutics and Pharmaceutical Sciences (ICPPS) organized by The Faculty of Pharmacy
Universitas Airlangga Surabaya lndonesia.
The proceeding was produced based on papers and posters presented at The
1st
lnternational
Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS), held in Surabaya, lndonesia, 14-
15November2014.
The proceeding clearly reflects broad interest, from the participants that coming from all around the
world.
The papeJ$presented were pharmaceutics and biopharmaceutics; requirements on howto evaluate
molecufp! in discovery and their appropriateness for selection as potential candidate; their
development in context of challenges and benefits, together with associated time and cost
implications and also requirements to progress through pre-clinical and clinical.
ln this an opportunity, lwould like to express my appreciation to the editorialteam of the proceeding
who have been working hard to review manuscripts, and making the first edition of this proceeding
be possible.
I would like also to thanks to all invited speakers and presenters who participated in The 1st
lnternational Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS) and your
contribution to this proceeding.
Finally, I hope this proceeding will give contribution
Sciences research.
Chairman,
Dra. Esti Hendradi, MSl., Ph.D., Apt
to the Pharmaceutics and Pharmaceutical
COMMITTEE
Advisor
Chairman
Editor
Universitas
: Dean of Faculty of Pharmacy
Ph'D' APt'
: Dra. Esti Hendradi, M'Si"
Airlangga
(Netherlands)
: Dr. Wouter U. Hinrichs
(JaPan)
Prof. Kozo TakaYama
Mutalik (lndia)
or. ttuttakorn Baisaeng {Thailand)
(lndonesia)
Prof. Dr. WidiiSoeratri' DEA
Dr. Srinivas
n
,rtn
Committee:
APt'
Prof. Dr. Widji Soeratri, DEA'
APt'
Prof. Dr. Siswandono, MS''
APt'
Prof .Dr. Tutuk Budiati' MS''
Yuwono' MS'' Apt'
Prof. Dr. rer'nat' Muhammad
APt'
Drs. Hadi Poerwono, MSc''Ph'D'
MSi'' Ph'D' Apt'
Drs. Marcellino Rudyanto'
APt'
Drs. Bambang Widjaja, M'Si'
Drs. SugiYartono, MS, APt'
APt'
Dra.Tutiek Purwanti, MSi'
APt'
Dra.Tristiana Erawati, MSi'
Dra.Retno Sari, MSc, APt'
Dra.Dewi lsadiartuti, MSi' APt'
Dra.Noorma Rosita, MSi' APt'
APt'
Dr. DwiSetyawan, SSi, MSi"
Primaharinastiti' SSi' MSi' Apt'
M'Phil'' Ph'D' Apt'
Dewi MelaniHariyadi' SSi'
APt'
HelmY Yusuf, SSi, MSc'' Ph'D'
AptDwi Lestari' SS|'MPharmSci''
Rrdhani
Dr. rer. nat. wtaria l-ucia
M'Sc' Apt'
tvt"lanny lka Sulistyowati' SFarm'
Apt' - '
Febri Annuryanti, SFarm'M'Sc'
M. Faris Adrianto, MFarm' APt'
Dini Retnowati, SFarm,APt'
Abhimata P, SFarm, APt'
Or.
ni"tt.
TABLE ofCONTENT
Preface from Chairman
Committee
Table of Contents
Author
...... ii
....--..--..--.
................. iii
lndex
.................... iii
AUTHQR INDEX
COMPARISON OF SODIUM STARCH GLYCOLATE AND CROSSCARMELLOSE SODIUM AS
SUPERDISINTEGRANT IN MEFENAMIC ACID FAST DISINTEGRATING TABLET
Adeltrudis Adelsa D, Oktavia Eka Puspita, Amalia Ayuningtyas, Marulita lsadora ,..........,........ 1
STUDY EXPRESSION OF HUMAN ERYTHROPOIETIN EXPRESSSION IN MAMMALIAN CELL
Adi Santoso, Popi Hadiwisnuwardhani, Yana Rubiana, Yulaika Romadhani, Endah Puji
Septisetyani, Dyaningtyas D.P. Putri
ANTIOXIDANT STABILITY ASSAY OF ALPHA TOCOPHERYL ACETATE IN SOL]D LIPlD NANOPARTICLE
SYSTEM (LlPlD BASE BEESWAX AND MONOSTEARIC GLISERYL
50:50)
Anggie Widhi, Noorma Rosita, Widji Soeratri ......................
A BIOACTIVE BOVINE HYDROXYAPATITE4ELATIN IMPLANT FOR IN VITRO GENTAMIC]N RELEASE
..... 13
Aniek Setiya Budiatin, M. Zainuddin, Junaidi Khotib, Diah Himawati
EFFECT OF COMPARISON SURFACTANTAND COSURFACTANT IN WATER/OIL MICROEMULSION IN
OVALBUMIN Microemulsion Water/Oilwith Surfactant (Span 8O-Tween 80) :
C-osurfactant (Ethanol) =5:L,6:!, and 7:1)
................. 18
Anisa Rizki Amalia, Riesta Primaharinastiti, Esti Hendradi ........,......,
RELEASE OF
ANALYSIS OF MYCOLIC ACIDS CLEAVAGE PRODUCT OF
Mycobacterium tuberculosis
CHROMATOGRAPHY-FLAM E IONIZATION DETECTOR
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Zainuddin
PERIPLASMIC EXPRESSION OF GENE ENCODING ANTI-EGFRvIIISINGLE-CHAIN
ANTIBODY USING PelB LEADER SEQUENCE lN ESCHER/CHIA COLI
BY GAS
.............. 21
VARIABLE FRAGMENT
lGrtika Sari Dewi, Debbie Sofie Retnoningrum, Catur Riani, Asrul Muhamad Fuad ................24
IN VIVO ANTIMALARIAL ACTIVIry OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION OF
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Aty Widyawaruyanti, Uswatun Khasanah, Lidya Tumewu, Hilkatul llmi, Achmad Fuad Hafid,
Indah S Tantular ...............
..................... 30
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Azza Faturrohmah, Arie Sulistyarini, Ana Yuda
...........
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BambangWidiaia, Achmad Radiaram, Arafah Zulhana
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HIDROXYPROPYL'P-CYCLODEXTRIN
"""""""""
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37
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Khotib
^a
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&
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-!-:r:- .--^.r.-L.,..*inah^c
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MUCOADHESIVE TABLET OF ETHANOLIC EXTRACT
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.
OF L-(3,4-Dichlorobenzoyl)-l',3-dimethylurea
MOLECULAR MODELTNG AND SYNTHES]S
' ;;"';;;
r""-t-"riur*"
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Purwanto
59
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53
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(EICUIhET|NC
FROM BULBS OF BAWANG TIWAT
EVALUATION OF ANTIHYPERURICEMIC ACTIVITY
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""""'
"""""72
OF COMBINATION OF STRAWBERRY FRUIT
ANTIOXIDANT ACTIVITY OF Xil%ETHANOL EKTRACT
L') uslNG ABTS FREE RADICAL
(Fragoria x anan(rssa Duch.) AND STARFRU 11 (Averrhaa carambola
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"""""""""' -?
'o
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ATORVASTATIN BY MICROCRYSTALLIZATION
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INVITR}ANTIMALARIALACTIVITYoFDIcHLoRoMETHANESUB.FRACTIoNoFEuco/yptusglobulus
L. Stem AGAINST Plasmodium folciporum
""""""""' 85
Elis Suwarni, Achmad Fuad Hafid, Aty Widyawaruyanti
ATcangelisiaflovaINCREASESRATS.LEUKoCYTESBUTHASBIPHASICEFFECToNRATS'LYMPHoCYTE
' ina"f puspiiasari,
Evi Umayah Ulfa,
vita Ariati, Mohammad sulthonHabibi
""""""""""""
89
Conierence
& Pharmaceutical Sciences
Arcangelisia flava INCREASES RATS, LEUKOCYTES
BUT HAS BIPHASIC EFFECT ON RATS' LYMPHOCYTE
tL e_
3rTri
us.
@
rcPtfuol4
lLil*
frsari.
Faculty of Pharmacy University of Jember; Jl. Kalimantan 37 Jember, lndonesia, e.puspitasari@
Frf l'.tmavah Ulfa, Faculty of Pharmacy University of Jember, il. Kalimantan 37 jember, lndonesia;_@
ulillrr c: Pharmacy University of Jember, Jl. Kalimantan 37 Jember, lndonesia;
Mohammad Sulthon Habibi.
4ar,nary University of Jember, Jl. Kalimantan 37 Jember, lndonesia.
gJ\ e
si
€?tli-S
altlr
Linerg5.
992
le
to4am
rd
untl
rich of natural resources, esperrdicinal plant, lndonesia has many
to
.rr oe fr
ll6r l
)€ h{illl
rtia C
Medamrc-
32'
Fdu-aqr
Reorn:
tK6
epCI1
develop medicinal sources
fiom plants. One of the potential
:'lant used for cancer chemopreagpnt is Arcangelisia flava. This plant
to have antioxidant and cytotoxic
aganst breast cancer cell line, MCFwere considered to be corre=nr[ity
:D the alkaloid content, especially
Keawpradub et al., 2005). As this
E
=ry to be found in Meru Betiri Na-
re
;itr.rated in Jember (Koran Jakarta,
are eager to explore further about
mreloped as medicine, we have to
;afety. The sub-chronic toxicity assay
to study the safety of ethanolic
nf 4- flava leaves (EEAfL) use. This re,mms Cone to determine the EEAfL
effect
,les dnd lymphocytes cell count of
sub chronic EEAfL.
;ll was done to determine the EE:'r rmmune response, especially on
;rd lymphocytes cell count of rats
,;l
chronic EEAfL.
AND METHODS
.=1s and extraction
frmr: ieaves were collected from Meru
Park, Jember, lndonesia. They
le::ed for their freshness, old age,
r on€s. The leaves were washed
M::nal
I
n
thoroughly with water, then, were air dried
followed by oven drying at 50 0C" The dried
leaves were grounded and sieved. The etha-
nolic extract were prepared using 500 g of
leaves powder according to the previous
study with a slight modification (Keawpradub
et al., 2005). The ground-dried leaves was
sequentially extracted with n-hexane, chloroform, and ethanol. The extraction was repeated three times for each solvent. The ethanol
extract was evaporated under reduced pressure (Heidolph, Laborota) resulting EEAfL. EEAfL was then suspensed in CMC Na 1% before
being administered to the animal.
Animals
Male Wistar rats (weighing 100-150 g) were
housed at a constant temperature and lightdark cycle. Rats were fed with standard feed
and water as libitum. The rats were acclima-
tized and quarantined for at least 10 days
prior to the experiment. The animal handling
protocols of this study were in accordance
with the guidelines of the animal care of University of Jember.
Experimental design
Fifteen rats were devided into three groups.
Group I as control, received CMC Na 1 %.
Group ll received EEAfL 500 mC/kC BW Group
lll received EEAfL 1,500 mglkg BW The treatment was done orally for 11 days. At the 12th
day, the blood sample was collected and analyzed further for leukocytes and lymphocytes
cell count.
Statistical analysis
All data were presented as mean + the stan-
koceeding
@rcpfuor4
nrelofrternational
on Pharmaceutics & pharmar
dard error of the mean (SEM). Kruskal
Wallis
assay followed by Mann Whitney
test were
used
Discussion
to increase the
of normal rats, as it was
cytes cell count. But it had
lymphocytes, as the higher the
EEAfL seems
to know the significance difference be_
tween groups, since the data could
not meet
the requirement for Anova analysis (p <
0,05).
the lymphocytes cell count. We
high dose of EEAfL decreased
system as it decreased the
AND DISCUSSION
Leukocytes cell count
The leukocytes cell count (Figure
1) of rats re_
ceiving EEAfL were increased significantly
than
that ofthe control group, either at the
dose of
500 or 1,500 mglkg BW
Lymphocytes cell count
The lymphocytes cell count of rats
receiving
RESULTS
count.
EEAfL contained berberine (pusp
Ulfa, 2013). The higher the dose,
the berberine content. Berberire
rmmunosuprressive agent. lt
tivation and proliferation of T
cytotoxic effect known (Xu et aL I
AfL might increase the non-spesft
system. Leukocytes consist of
500 mg/kg BW EEAfL was increasing
signifi_
cantly, but the rats treated with EEAiL
ai tn"
dose of 1,500 mg/kg BW had U".r""r"J
tyr_
phocytes (Figure 2).
lymphocytes, monocytes,
basophyls. Lymphocytes plays rob
rmmune response, while others d
specific immune response.
Leukocytes cells count that was
while the lymphocytes cell count
creasing after treated with high
-2(m
g 1ym
Er(}m
-
rgrorp
I
f6roup
I
qgrsup ilt
5m0
suggested that the other kinds
0
involving
2mm
CONCLUSION
1Sm
the results, we can
EEAfL increased the immune
rats, but it had biphasic effect
on
suggesring that high dose of EEAfl_
creased non-specific immune
Based on
1S0O
5 1SS
: lDS
g
1lxf,F
48ffi
€ oinc
E
i 400
lffu
agrolp
I
ilgralp
tr
stead
o
Figure 2. LymphocyJ*,, count
of rats. Data
represented as mean + SEM (n
= 5). Different
letter n otatio n exp ressed, ign in.a
ni O iff*r"
according to Mann Whitney test (p
< 0,05).
'*,'
i
u,* *
d
non-specific immurp
were higher. Water extract of A.
macrophage activity (Florentina,
EEAfL might also increase the m;
tivity. Still, we need to examined
these hypothesis.
Figure 1. Leukocytes cell count
of rats. Data
represented as mean + SEM (n
= 5). Different
letter n otatio n exp ressed s ign ifi
ca ni d iff" r" n."
according to Mann Whitney test (p
< 0,05).
:.
in
-
'-
]
*-".'
33"t'." o''."rou, :'; ,r-f .r,u**
ofthe specific one.
REFERENCES
*u
1..
Florentina, D. (2013). pengaruh
Larut Air Kayu Kuning (Arcangelisiel
(1.) Merr.) terhadap Fagositosis
tt*
rofag secara ln Vitro pada Tikus
l
s-"r,::i:;,
'tL$,:it1--;1i:#
$;r_li{i:'rli1i+tr*.1r!
l'.1*,:jil9+!,, i'
.i.,:..:iii:, :.,:1ii::r1..,
.
r
: F,E::+;:,,
hoceeding
tblo
herndionat
qr
I
Gonf eneme
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Keawpradub, N., Dej-adisai, A., and
nyongsawad, S. (2005).
Cytotoxic Activities of Thai
Puspitasari, E. and Ulfa, E.U' (20L3).
Pengembangan Ekstrak Etanol Ar
cangelisia flava Terstandar sebagai Agen
Pendamping Kemoterapi Doxorubicin
untuk Pengobatan Kanke; Laporan Ta
NartsNamedKhaminkhruea:Arcangeli
hunanPenelitian,LembagaPenelitian
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saftr
f,uran Jakarta (2}1I2l. Peneliti
@27-
e
&,ntikanker
€r
s
rhc
ffi[
E
fie'
tln
,laY
b
hat
rse
cf
f
le
nEl
20131.-
d fu!+5ir
onclttde
respcne
Aft
Temukan
di Meru Betiri, Koran Ja
fngital Edition, http://kora n-jakar
tse
m-gfn
resporse
aruhr
Ekfd
lngelliia
tosis
h|l"ar
Tikus Gatur
@
rcP#p2o*4
.phpldetail/viewO1/
diakes 14 Maret 2013
Universitas Jember'
Xu, L', Liu, Y., and He, X. (2005)' lnhibi
tory Effects of Berberine on The Action
and Cell Cycle Progression of Human Pe
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