The effect of nickel as a nickel chromium restoration corrosion product on gingival fibroblast through analysis of BCl-2
202
Volume 45 Number 4 December 2012
Research Report
The effect of nickel as a nickel chromium restoration corrosion
product on gingival fibroblast through analysis of BCl-2
fX ady Soesetijo1 and Mandojo rukmo2
Department of Prosthodontics, Faculty of Dentistry, Universitas Jember, Jember – Indonesia
2
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Airlangga, Surabaya – Indonesia
1
abStract
background: Restoration of NiCr may undergo corrosion process in artificial saliva. Corrosion product is soluble Ni substances
in salivary electrolytes. Ni2+ may freely enter the cells through passive transport DMT-1. Ni2+ in the cell causes initiation of the ROS
formation,which subsequently can conduct the redoxs reactions leading to DNA damage. The damage DNA affects the genetic expression,
especially bcl-2, and even triggers apoptosis. Purpose: The aim of this study was to reveal the mechanism of Ni toxicity as a corrosion
product of NiCr restoration on gingival fibroblasts through expression analysis of Bcl-2. Methods: Cells with a density of 105 planted
on each coverslip in 72 wells to the treatment group and 24 wells to the control group (24 hours incubation). In the treatment groups,
each well exposed with 20 μL artificial saliva containing Ni concentration results immerse each restoration, whereas the control group
was exposed to 20 μL artificial saliva (incubation 1, 3, and 7 days). The data collected were subsequently analyzed using two-ways
ANOVA, followed by one-way ANOVA. Comparing between experimental groups after one-way ANOVA was conducted using Fisher’s
LSD. Whereas, the calculation and documentation of Bcl-2 expression was performed camera of Olympus Microscope BX-50 Japan.
results: Statistical analysis of two-ways ANOVA showed the presence of interaction between the increasing Ni concentration and
exposure duration on the expression of Bcl-2 gingival fibroblasts (p=0.021
Volume 45 Number 4 December 2012
Research Report
The effect of nickel as a nickel chromium restoration corrosion
product on gingival fibroblast through analysis of BCl-2
fX ady Soesetijo1 and Mandojo rukmo2
Department of Prosthodontics, Faculty of Dentistry, Universitas Jember, Jember – Indonesia
2
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Airlangga, Surabaya – Indonesia
1
abStract
background: Restoration of NiCr may undergo corrosion process in artificial saliva. Corrosion product is soluble Ni substances
in salivary electrolytes. Ni2+ may freely enter the cells through passive transport DMT-1. Ni2+ in the cell causes initiation of the ROS
formation,which subsequently can conduct the redoxs reactions leading to DNA damage. The damage DNA affects the genetic expression,
especially bcl-2, and even triggers apoptosis. Purpose: The aim of this study was to reveal the mechanism of Ni toxicity as a corrosion
product of NiCr restoration on gingival fibroblasts through expression analysis of Bcl-2. Methods: Cells with a density of 105 planted
on each coverslip in 72 wells to the treatment group and 24 wells to the control group (24 hours incubation). In the treatment groups,
each well exposed with 20 μL artificial saliva containing Ni concentration results immerse each restoration, whereas the control group
was exposed to 20 μL artificial saliva (incubation 1, 3, and 7 days). The data collected were subsequently analyzed using two-ways
ANOVA, followed by one-way ANOVA. Comparing between experimental groups after one-way ANOVA was conducted using Fisher’s
LSD. Whereas, the calculation and documentation of Bcl-2 expression was performed camera of Olympus Microscope BX-50 Japan.
results: Statistical analysis of two-ways ANOVA showed the presence of interaction between the increasing Ni concentration and
exposure duration on the expression of Bcl-2 gingival fibroblasts (p=0.021