PENGARUH SUPLEMENTASI VITAMIN E BERSAMA DENGAN PEMBERIAN DEKSAMETASON TERHADAP GAMBARAN HISTOPATOLOGI JARINGAN PARU-PARU TIKUS PUTIH JANTAN (Rattus norvegicus).
PENGARUH SUPLEMENTASI VITAMIN E BERSAMA DENGAN
PEMBERIAN DEKSAMETASON TERHADAP GAMBARAN
HISTOPATOLOGI JARINGAN PARU-PARU
TIKUS PUTIH JANTAN (Rattus norvegicus)
SKRIPSI
Diajukan untuk Melengkapi Tugas-Tugas dan Memenuhi
Persyaratan untuk Mencapai Gelar Sarjana Kedokteran Hewan
Oleh
Bina Ichsantya
NIM.1109005036
FAKULTAS KEDOKTERAN HEWAN
UNIVERSITAS UDAYANA
DENPASAR
2015
PENGARUH SUPLEMENTASI VITAMIN E BERSAMA DENGAN
PEMBERIAN DEKSAMETASON TERHADAP GAMBARAN
HISTOPATOLOGI JARINGAN PARU-PARU
TIKUS PUTIH JANTAN (Rattus norvegicus)
SKRIPSI
Diajukan untuk Melengkapi Tugas-Tugas dan Memenuhi
Persyaratan untuk Mencapai Gelar Sarjana Kedokteran Hewan
Oleh
Bina Ichsantya
NIM.1109005036
Menyetujui/Mengesahkan:
Pembimbing I,
Prof. Dr. drh. I Ketut Berata, M.Si
NIP. 19610914 198702 1 001
Pembimbing II,
drh. Samsuri, M.Kes
NIP. 19710405 199903 1 003
DEKAN FAKULTAS KEDOKTERAN HEWAN
UNIVERSITAS UDAYANA
Dr. drh. Nyoman Adi Suratma, MP.
NIP. 19600305 198703 1 001
Tanggal Lulus :
Setelah mempelajari dan menguji dengan sungguh-sungguh kami
berpendapat bahwa tulisan ini baik ruang lingkup maupun kualitasnya dapat
diajukan sebagai skripsi untuk memperoleh gelar Sarjana Kedokteran Hewan.
Ditetapkan di Denpasar, Tanggal .......................
Panitia Penguji :
Prof. Dr. drh. I Ketut Berata, M.Si
Ketua
drh. Samsuri, M.Kes
Sekretaris
drh. Luh Made Sudimartini, M.Sc
Anggota
Dr. drh. Ida Bagus Oka Winaya, M.Kes
Anggota
drh. A.A.Gde Oka Dharmayudha,MP
Anggota
DAFTAR ISI
Halaman
RIWAYAT HIDUP .................................................................................................i
ABSTRAK ................................................................................................................ii
ABSTRACT .............................................................................................................iii
UCAPAN TERIMAKASIH ....................................................................................iv
DAFTAR ISI .............................................................................................................vii
DAFTAR TABEL ...................................................................................................ix
DAFTAR GAMBAR ...............................................................................................x
DAFTAR LAMPIRAN ...........................................................................................xi
BAB I. PENDAHULUAN ........................................................................................1
1.1 Latar Belakang........................................................................................1
1.2 Rumusan Masalah ..................................................................................3
1.3 Tujuan Penelitian ...................................................................................3
1.4 Manfaat Penelitian .................................................................................3
BAB II. TINJAUAN PUSTAKA ............................................................................4
2.1 Tikus Putih (Ratus norvevgicus) ............................................................4
2.2 Paru-Paru ...............................................................................................5
2.3 Deksametason .........................................................................................8
2.4 Vitamin E ................................................................................................10
2.5 Kerangka Konsep ..................................................................................11
2.6 Hipotesis .................................................................................................13
BAB III. MATERI DAN METODE ......................................................................14
3.1 Materi penelitian ....................................................................................14
3.1.1 Sampel penelitian ........................................................................14
3.1.2 Alat penelitian .............................................................................14
3.1.3 Bahan penelitian ..........................................................................14
3.1.4 Dosis vitamin E dan deksametason .............................................14
3.2 Variabel Penelitian .................................................................................15
3.3 Rancangan Penelitian .............................................................................15
3.4 Metode Penelitian ..................................................................................15
3.4.1 Perlakuan.......................................................................................15
3.4.2 Pembuatan sediaan histopatologi ..................................................15
3.5 Variabel yang Diperiksa ........................................................................16
3.6 Analisis Data ..........................................................................................17
3.7 Lokasi dan Waktu Penelitian .................................................................17
BAB IV. HASIL DAN PEMBAHASAN ...............................................................18
4.1 Hasil ......................................................................................................18
4.1.1 Pemeriksaan preparat ...................................................................18
4.1.2 Uji statistik ...................................................................................23
4.2 Pembahasan .......................................................................................... 26
4.3 Pengujian Hipotesis ............................................................................. 29
BAB V. SIMPULAN DAN SARAN .......................................................................30
5.1 Simpulan ...............................................................................................30
5.2 Saran ......................................................................................................30
vii
DAFTAR PUSTAKA ...............................................................................................31
LAMPIRAN .............................................................................................................35
viii
DAFTAR TABEL
Halaman
2.1. Tabel Data Taksonomi Tikus Putih .............................................................. 4
2.2. Tabel Data Fisiologis Tikus Putih ................................................................. 5
4.1. Tabel Hasil Pemeriksaan Lesi Degenerasi Parenkim........................................ 18
4.2. Tabel Hasil Pemeriksaan Lesi Perdarahan ........................................................ 18
4.3. Tabel Hasil Pemeriksaan Lesi Nekrosis ............................................................ 19
4.4. Tabel Rerata Nilai Kerusakan Paru-Paru Tkus Putih........................................ 23
4.5. Tabel Hasil Uji Kruskal-Wallis......................................................................... 24
ix
DAFTAR GAMBAR
Gambar
Halaman
2.1. Gambaran Mikroskopik Paru-Paru Normal ...................................................... 8
2.2. Struktur Deksametason. .................................................................................... 10
2.3. Struktur Vitamin E. ........................................................................................... 11
2.4. Kerangka Konsep. ............................................................................................. 12
4.1 .Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok
Kontrol Negatif (HE, 400x) .............................................................................. 19
4.2. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok
Kontrol Positif (HE, 400x) ................................................................................ 20
4.3. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P1
(HE, 400x)......................................................................................................... 21
4.4. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P2
(HE, 400x)......................................................................................................... 22
4.5. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P3
(HE, 400x)......................................................................................................... 23
4.6. Grafik Rerata Skor Histopatologi Paru-Paru Tikus Putih ................................. 26
x
DAFTAR LAMPIRAN
Lampiran
Halaman
1. Hasil Skoring Lesi Degenerasi Parenkim. ........................................................... 35
2. Hasil Skoring Lesi Perdarahan. ............................................................................ 35
3. Hasil Skoring Lesi Nekrosis. ............................................................................... 36
4. Hasil Analisis Data .............................................................................................. 37
5. Hasil Rerata Skoring pada Setiap Uangan .......................................................... 37
6. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Negatif
dengan Kontrol Positif ......................................................................................... 39
7. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P1 ............................................................................................................ 42
6. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P2 ............................................................................................................. 42
7. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P3 ............................................................................................................. 43
xi
PEMBERIAN DEKSAMETASON TERHADAP GAMBARAN
HISTOPATOLOGI JARINGAN PARU-PARU
TIKUS PUTIH JANTAN (Rattus norvegicus)
SKRIPSI
Diajukan untuk Melengkapi Tugas-Tugas dan Memenuhi
Persyaratan untuk Mencapai Gelar Sarjana Kedokteran Hewan
Oleh
Bina Ichsantya
NIM.1109005036
FAKULTAS KEDOKTERAN HEWAN
UNIVERSITAS UDAYANA
DENPASAR
2015
PENGARUH SUPLEMENTASI VITAMIN E BERSAMA DENGAN
PEMBERIAN DEKSAMETASON TERHADAP GAMBARAN
HISTOPATOLOGI JARINGAN PARU-PARU
TIKUS PUTIH JANTAN (Rattus norvegicus)
SKRIPSI
Diajukan untuk Melengkapi Tugas-Tugas dan Memenuhi
Persyaratan untuk Mencapai Gelar Sarjana Kedokteran Hewan
Oleh
Bina Ichsantya
NIM.1109005036
Menyetujui/Mengesahkan:
Pembimbing I,
Prof. Dr. drh. I Ketut Berata, M.Si
NIP. 19610914 198702 1 001
Pembimbing II,
drh. Samsuri, M.Kes
NIP. 19710405 199903 1 003
DEKAN FAKULTAS KEDOKTERAN HEWAN
UNIVERSITAS UDAYANA
Dr. drh. Nyoman Adi Suratma, MP.
NIP. 19600305 198703 1 001
Tanggal Lulus :
Setelah mempelajari dan menguji dengan sungguh-sungguh kami
berpendapat bahwa tulisan ini baik ruang lingkup maupun kualitasnya dapat
diajukan sebagai skripsi untuk memperoleh gelar Sarjana Kedokteran Hewan.
Ditetapkan di Denpasar, Tanggal .......................
Panitia Penguji :
Prof. Dr. drh. I Ketut Berata, M.Si
Ketua
drh. Samsuri, M.Kes
Sekretaris
drh. Luh Made Sudimartini, M.Sc
Anggota
Dr. drh. Ida Bagus Oka Winaya, M.Kes
Anggota
drh. A.A.Gde Oka Dharmayudha,MP
Anggota
DAFTAR ISI
Halaman
RIWAYAT HIDUP .................................................................................................i
ABSTRAK ................................................................................................................ii
ABSTRACT .............................................................................................................iii
UCAPAN TERIMAKASIH ....................................................................................iv
DAFTAR ISI .............................................................................................................vii
DAFTAR TABEL ...................................................................................................ix
DAFTAR GAMBAR ...............................................................................................x
DAFTAR LAMPIRAN ...........................................................................................xi
BAB I. PENDAHULUAN ........................................................................................1
1.1 Latar Belakang........................................................................................1
1.2 Rumusan Masalah ..................................................................................3
1.3 Tujuan Penelitian ...................................................................................3
1.4 Manfaat Penelitian .................................................................................3
BAB II. TINJAUAN PUSTAKA ............................................................................4
2.1 Tikus Putih (Ratus norvevgicus) ............................................................4
2.2 Paru-Paru ...............................................................................................5
2.3 Deksametason .........................................................................................8
2.4 Vitamin E ................................................................................................10
2.5 Kerangka Konsep ..................................................................................11
2.6 Hipotesis .................................................................................................13
BAB III. MATERI DAN METODE ......................................................................14
3.1 Materi penelitian ....................................................................................14
3.1.1 Sampel penelitian ........................................................................14
3.1.2 Alat penelitian .............................................................................14
3.1.3 Bahan penelitian ..........................................................................14
3.1.4 Dosis vitamin E dan deksametason .............................................14
3.2 Variabel Penelitian .................................................................................15
3.3 Rancangan Penelitian .............................................................................15
3.4 Metode Penelitian ..................................................................................15
3.4.1 Perlakuan.......................................................................................15
3.4.2 Pembuatan sediaan histopatologi ..................................................15
3.5 Variabel yang Diperiksa ........................................................................16
3.6 Analisis Data ..........................................................................................17
3.7 Lokasi dan Waktu Penelitian .................................................................17
BAB IV. HASIL DAN PEMBAHASAN ...............................................................18
4.1 Hasil ......................................................................................................18
4.1.1 Pemeriksaan preparat ...................................................................18
4.1.2 Uji statistik ...................................................................................23
4.2 Pembahasan .......................................................................................... 26
4.3 Pengujian Hipotesis ............................................................................. 29
BAB V. SIMPULAN DAN SARAN .......................................................................30
5.1 Simpulan ...............................................................................................30
5.2 Saran ......................................................................................................30
vii
DAFTAR PUSTAKA ...............................................................................................31
LAMPIRAN .............................................................................................................35
viii
DAFTAR TABEL
Halaman
2.1. Tabel Data Taksonomi Tikus Putih .............................................................. 4
2.2. Tabel Data Fisiologis Tikus Putih ................................................................. 5
4.1. Tabel Hasil Pemeriksaan Lesi Degenerasi Parenkim........................................ 18
4.2. Tabel Hasil Pemeriksaan Lesi Perdarahan ........................................................ 18
4.3. Tabel Hasil Pemeriksaan Lesi Nekrosis ............................................................ 19
4.4. Tabel Rerata Nilai Kerusakan Paru-Paru Tkus Putih........................................ 23
4.5. Tabel Hasil Uji Kruskal-Wallis......................................................................... 24
ix
DAFTAR GAMBAR
Gambar
Halaman
2.1. Gambaran Mikroskopik Paru-Paru Normal ...................................................... 8
2.2. Struktur Deksametason. .................................................................................... 10
2.3. Struktur Vitamin E. ........................................................................................... 11
2.4. Kerangka Konsep. ............................................................................................. 12
4.1 .Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok
Kontrol Negatif (HE, 400x) .............................................................................. 19
4.2. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok
Kontrol Positif (HE, 400x) ................................................................................ 20
4.3. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P1
(HE, 400x)......................................................................................................... 21
4.4. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P2
(HE, 400x)......................................................................................................... 22
4.5. Gambaran Histopatologi Paru-Paru Tikus Putih pada Kelompok P3
(HE, 400x)......................................................................................................... 23
4.6. Grafik Rerata Skor Histopatologi Paru-Paru Tikus Putih ................................. 26
x
DAFTAR LAMPIRAN
Lampiran
Halaman
1. Hasil Skoring Lesi Degenerasi Parenkim. ........................................................... 35
2. Hasil Skoring Lesi Perdarahan. ............................................................................ 35
3. Hasil Skoring Lesi Nekrosis. ............................................................................... 36
4. Hasil Analisis Data .............................................................................................. 37
5. Hasil Rerata Skoring pada Setiap Uangan .......................................................... 37
6. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Negatif
dengan Kontrol Positif ......................................................................................... 39
7. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P1 ............................................................................................................ 42
6. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P2 ............................................................................................................. 42
7. Analisis Statistik Perbandingan Skoring Kelompok Kontrol Positif
dengan P3 ............................................................................................................. 43
xi