Ornamentation Structure of Flower Pollen on Entomophyli Pollination.

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lnternational Conference on Biosciences and Biotechnology "Pave the Way to A Better Life"

Content

No
Preface

Forewords-Rector of Udayana UniversiW
Table of Content

Pase
11

ur

KEYNOTE PRESENTATION
IN

WTRO BREEDING STRATEGIES IN THE

DEVELOPMENT OF NATIVE PLANTS

USE OF

1

KP-1

Acram Taji and Richard Williams
2

STEM CELL AND ITS MICROENVIRONMENT
Ferry Sandra

Y\P-2

J

THE DEVELOPMENT OF REVERSE GENETIC TO DEVELOP
VACCINE TO CONTROL BIRD FLU IN POULTRY IN

INDONESIA
I Gusti Ngurah Mahardika, I Nyoman Suartha, and Melina Jonas

KP-3

4

Review
GENE ISOLATION BY USING TRANSPOSON AND T-DNA

KP-4

TAGGING METHODS
I G.K. Susrama, I G.N. Bagus, and I G.P.Wirawan
5

WHY ARE T}IE NETWORKS FOREST ECOSYSTEM?
FROM THE BIOLOGY OF ARMILLARII AND TERMITOMYCES
Jooyoung Cha and IGP Wirawan


KP.lO

6

ONLINE SIMULATION OF BIOPROCESSES
Klaus-Uwe Gollmer

KP-l1

7

ELICITORS INDUCING PLANT DEFENSE RESPONSES
Kazuhito Kawakita

KP-14

8

THE CONTROL OF GONADOTROPIN-RELEASING HORMONE
(GNRH) IN MAMMALS: A WAY TO IMPROVE THE FERTILITY

IN DOMESTIC ANIMALS
Kei-ichiro Maeda, Kinuyo Iwata, Yoshihisa lJenoyama, Satoshi
Ohkura and Hiroko Tsukamura

KP-23

9

CONSERVATION ACTIVITIES OF AN ENDANGERED ANIMAL
Mitsuaki Ogata

KP-29

l0

CELL WALL DEGRADATION AND MODIFICATION ENZYMES
OF GRAM- POSITIVE BACTERIA: HISTORY, IMPORTANCE
AND FUTURE ASPECTS
Sekiguchi J.


KP-33

11

STRUCTURAL ANALYSIS OF THE PHOTOREACTIONS OF
FLAVIN-BINDING PROTEINS BY FTIR SPECTROSCOPY
Tatsuya Iwata

KP-36

Bali, lndonesia 123-24 September 2010

i

ffir"o

10

lnternational Conference on Biosciences and Biotechnology "Pave the Way to A Better Life"


STUDYABOUT INDONESIAN BLUE BOTLE JELLYFISH
(PhysatiaPftrsalrs)VENoM FROM THE WATERS OF PAPUMA
JEMBER

oH-35

AlMunawir
11

oH-36

TNFECTTVTTY OF LYTIC PHAGE TO EPEC

(ENTEROPATHOGEMC ESCHERICHIA COLI ) FROM
DIARRTIEAL PATIENTS IN INDONESIA
Sri Budiarti

t2

13


ANTTEECTERIAL EFFECT OF LACTOFERRIN AND ,
LACTOFERRIN IIYDROLYZNE ON Enterobacter s akazakii
Fatma ZuhrotunNisa, Hafsyah Laili Nurwandari and Elza Ismail

oH-37

,q.NOVgL OF REPLACING CACO-2 CELL WITH ENTEROCYTE
MICE TO DETERMINE BACTERIAADHESION ACTIVITY IN
VITRO
Sukrama,ID. M.

oH-38

POSTER PRESENTATION : AGRICULTURE

M

STRUCTURE OF FLOWER POLLEN ON PA-1
ENTHOMOPI{YLI POLLINATION

Ni Putu Adriani Astiti

I

2

J

WErc+IT LOSS AND RESPIRATION RATE OF SALACA FRUIT
IN MODIFIED ATMOSPHERE USING POLYETHYLINE PLASTIC
PACKAGING AT VARIOUS PERFORATION
Ida Ayu Rina Pratiwi Pudja

PA-4

SOYBEAN (Glycine max (L) MERRILL) in planta

PA-8

HOW TO USg AND TREAT THE LAND ACCORDING TO VEDIC


PA-l1

TRANSFORMATION OF SUNFLOWER ALBUMIN GENE USING
A gro b a ct erium tumefo c i e ns
I Wayan Suberataand I Putu Suparthana
4

KNOWLEDGE AND AUTHORITY
Wayan Suena
5

POST HARVEST MANAGEMENT OF GLADIOL (Gladiolus

PA-15

hybridus) AS CUT FLOWER
Made Ria Defiani
6


CTTNOUOSOMES OBSERVATION ON CULTIVARS OF

BTASSiCA

PA-18

napusL.
tvtade Pharmawati,
7

A.A. Gde Indraningrat, Ni Nyoman Wirasiti

RESPONSE OF OTT'BNTNC PANCREAS EXTRACT AND RATION

PA.22

SUPLEMENTED BY PROBIOTIC ON CARCASS, BLOOD SUGAR
CONCENTRATION, AND BLOOD LIPID PROFILE TO BROILER
lokorda Gede Belawa Yadnya and Anak Agung Ayu Sri Trisnadewi
Bali, lndonesia 123-24 September 2010


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Conference on Biosciences and Biotechnology

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ORNAMENTATION STRUCTT]RE OF FLOWER POLLEN ON
ENTHOMOPITYLI POLLINATION
Ni Putu Adriani Astiti
adrianiastiti@yahoo. co. id
Biology Department, Faculty of Mathematic and Natural Science, Udayana University

ABSTRACT
Research has been carried pollen from many plant species with the
afun to find out from the wall
,
of pollerr motphology in the interest pollination caried
by insects (entomophyli) .Materiai taken
from plants belonging-to Monoicotyledoneae and Dicotyledoneae. preparation
was done by
acetolysis method and observation using light microscopy.
Fro* the.esults showed that the pollen
in flowers polination carried by insects (Jrtomophyli)';*"ruily
h;r" a flat surface, a bit rough
with the noise - kind of ornamentation.ornamentation in
the iorm of spine or sculpture with a
different type for each species.

Key word: Pollen, Entomophyli, ornamentation

INTRODUCTION
Flowering is one aspect of plant life. Shape and color
of the beautifirl flowers, a
neat header regular interest, and the smell of fragrant
fl;";r
that invite insects to come
looking for honey .Fruit and seed crops depend "on pollination
results. Insects can cause
pollen move from one flower to another flower.
wh; th";;ii;" is already carried by the
fal,l on the stigma, it happens pollination. Once the
importance of the role of insects in
pollination, it is interesting to note in particular
flower pott"n mo.phological structure, as
in; pollen pollination plays an important role especiaffv
m tfrr*ent of conception.
The beauty of.colors, flower shapes, as well as a special
smell and produce honey
or nectar to attract different tlpes of insects, so the animals
- these animals often visit
flowers and pollinating accidentally (Arnet and Braunga
rt, tgtlo).
Important role as a pollinator insects and p--ollen types
are shown variations

associated with the visited flower. Some insects are
irelpful iir'pollination are bees, flies,
wasps, butterflies and beetles (Bhojwani and Bhatnag
ar,'r9r7g1-.'
Pollen produced by terrestrial plants is the prlvii"g"
oi d".r"lopment resistance of

exterior walls. The outer wall is eksin that contain
*u*
helps the wax layer scattering. Pollination interest
carriea by insects, the pollen surface
ornamentation spine and there are carved images to
assist in tire scattering (Kapp, 1969).

* ,"; H;i-;fa"*-J.irrr,

MATERIALS AND METIIODS
The samples taken were from the groug Monocotyledoneae
and Dicotyledoneae,
respectively - each five species' The group of monocotyLaor*"
is Lilium longiflorurn,
lladiolus sp, Amaryllis sp, sp Arachnis an-d vanda rp, *irrr"
of dicotyledoneae

*"0
is
caesalpinia pulcherrima, crotararia sp, sesiania groidiltoro
pers, Hibiscas
rosasinensis and Nymphaea sp. preparation was
uv

dine
acetolysis rrr"rrroa
983).
Pollen taken from anthers collected in a test tube (vial),
which is already filled with
glacial acetic acid, then left for 24 hours. Then transfrerred'into
a centrifuge tube, and
centrifugation performed. The liquid was removed and
replaced with glacial acetic acid
(Soerodikoesoemo,

1

with concentrated sulfuric acid with the ratio 9: l.Heating in
a water bath from room
temperature to boiling. After boiling heating was stoppeiand
settling for 15 minutes.
Subsequently conducted centrifuge, then the liquid *u*
and replaced with
distilled water. observation wittr tigtrtmicroscope, was still
dark when 6r"r"rri"g i,

,*rred

Bali, lndonesia 123-24 September 2010

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lnternational Conference on Biosciences and Biotechnology "Pave the

drops 2-3' drops of HCI'
carried out using glacial acetic acid 2 cc of sodium Chlorates 2-3
the sediments were washed
and then centrifugation. Furthermore, the liquid removed and
Last
with distilled water 2-3 times, with each iaundering conducted centrifuge again. with
and mixed
distilled water was ,eptac"O with glycerin jelly that has been heated
microscope'
safranin dyes.Further observation with light

For the identification carried out:
- Mesurement of polar axis, and Equatorial'
-Determining of index P/E
-Determine lhe type of pollen (by Erdtman,1952)'
-View pollen ornamentation on the walls'
photo shoot done using olympus brand camera c 35 A, 100 ASA Kodak film.

RESULTS AND DISCUSSION
From 10 species observed were obtained the foltowing results:

'

ra

be'

l

r

'"0*
il1:1";H,:l;,r'fi ffil:ITfl i:f:i;fi

Alaaislatq I

r,

,.ii

2
!.:

ULt4alusifr@n

4

V

'

: .#.lrt
i-

c+!st0.165 l
tsro,,
:r

i

E7.531r.413 ?4.E9t0.s65

Amuryllis

:

,:il

Vanddsp

j::ilii,,lrs
I

rrq19!,

fi:

33.rt11.5t

l=1ffiI

30.336i0.707

?q:P-.i-.q199.j....!s*0:0

rrir-,U=:::riir:.rrilf.rer*.0:.0..0-,,,,,,
:lr
,ii:..;;1??:l::?iiil;:,,:,:,rli?::,=,11inT,:,:*ri7li,:
i:=iilii:li
':
\; ':
., ',-

6

cmiii;oq..
--r
,1+ , "';'
E

E
l0

'

r,:::rrl

Lri:1ii

:
6t't910.t65 l'Mt 0'013
23Jt!1J22 re'iC 1r'r2: 1j t'2t10'016

ii*itpi"iopuru,*l.d 7r.731 l.E0l

r;
'
i.iiiiii""aU-o Pd 30.s1!t'113 27'4elt'4t'
",' -' rs';r1i.lr3 3r.92it'713-"
;,1 Nlmphaaq
',,
;
' :' .';'' tf"'''''riiiiiJ*iii'n*a"
,ii
t2'40i1'400
E2.40!t.sr7

r'1f,!0'007

l'12!0'017

jr-

l'00t0'000

sp'O:yl!::,t::::
Figure t.
' Gladiolus
grandiflora
Sesbania
sp,
Crotalqria
yanda
pulcherrima,
Ciesalpinia
sp,
Arachnis and
Pers, Hibiscus rosasinensis and Nymphaea sp'

iio**

O*urn"

Tabel 2. Pollen description of species

Bali, lndone sia t 23-24 September 2010

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lnternational Conference on Biosciences and Biotechnology "Pave the Way to A Better Life,,

Amarillis sp.

Kolpus a rather long and coiled, type subprolata,
rough surface is full of warts (verucate)
Kolpus lengthwise on a polar axis, ellipse, type
subprolata, ornamentation in the form of a small

Lilium longiflorum

Kolpus lengthwise on

spine and short on the surface

Vanda sp.

Arachnis sp.
C ae s alp

inia pu I ch errima

a polar axis, ellipse, type
subprolata, ornamentation in the form of a small
spine - small and short on the surface
Kolpus polar axis elongates, tpe prolata sferoidal,
rough surface is full of warts (verucate)
rotnded shape, ob)ate type sfen)dd - prdata
sferoidal, ornamentation is not clear
Having a third aperture and three porous, rough
surface with warts (verucate), tlpe prolata

sferoidal.

Crotalaria
Ses b

sp.

ania grandifl o ra

Nymphaea sp.

l0

Hibiscus rosasinensis

P

ers

Surface uneven, notched with three kolpus, type
subprolata
rough surface is full of warts (verucate), has three
kolpus, type prolata sferoidal
uneven surfaces, kolpus rather long, sferoidal
prolata type, ornamentation is not clear
ornamentation in the form of a long spine on the
surface (echinate), oblate type sferoidal - prolata
sferoidal.

ln general, pollen of flowers pollination carried by insects (entomophyli) has an
uneven surface, a bit rough, with exquisite omamentation such as spines, warts and the
presence of sculptures - sculpture such as nets. Type of individual - each a different

species, although included in the same family, such as Crotalaria sp, Sesbania
grandiflora Pers and Caesalpinia pulcherima, although all three are included in each type
of the Leguminosae family is different because each type of index P / E is different.
Likewise with Vanda sp and sp Arachnis which both belong to the family Orchidaceae,
also different types. But Gladiolus sp, sp and Lilium longiflofum Amaryllis sp, all three
including the order Liliales (Pudjoarianto and Budi Rahayu, 1977)have the same type of
pollen that is subprolata. Accordingly Index P / E is to determine the type of pollen from
each - each species. In Summary, Pollen on flower pollination done by insects
(entomophyli) has the structure: Uneven surfaces. Slightly rough surface .r /ith
ornamentation in the form of spine, the curve,warts and sculpture in the form of a net.
Type of each type differs depending on the index P / E.
REF'ERENCES
Amet RH and Braungart DC. 1970. An Introduction to Plant Biology. Third Edition. C.V. sby Company.
SaintLouis. Toppan Company, LTD. Tokyo, Japan. p. 223-224.

Bhojwani SS and Bhahragar SP. 1974. The Embryology of Angiosperms. Third Revised Edition. Vikas
Publishing Hous, P.V.T, LTD. p. 44 - 47 ,95 - 96.
Erdtman G. 1952. Pollen Morphology and Plant Taxonomy. Angiosperm (An Introduction to Palynology I).
The Chronica Botanica CO. Walrtham, Mass, U.S.A.
Kapp RO. 1969. How to Know. Pollen and Spores. WM.C. Brown Company Publishers.Dubuque. Iowa.
Pudjoarianto A dan Rahayu SB. 1977. Diktat Sistematik Tumbuhan IIL Jilid I. Seksi Sistematik Tumbuh
Tumbuhan. Fakultas Biologi, Universitas Gadjah Mada. Yogyakarta.
Soerodikoesoerno W. 1983. Petunjuk Praktikum Mikroteknik Tumbuhan. Lab. Embriologi Dan Mikroteknik
Tumbuhan. Fakultas biologi, Universitas Gadjah Mada Yogyakarta.

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Bali, lndonesia 123-24 September 2010

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