Test of Homogeneity of Variances

  Lampiran 1 Analisis statistik menggunakan SPSS 16.0

a. Data TPC bakteri pada senyawa hidrokarbon alifatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU TPC N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 8.3603 Std. Deviation 2.016 2.89047 2.89246

  Most Extreme Differences Absolute .125 .231 .283 Positive .125 .213 .156 Negative -.125 -.231 -.283

  Kolmogorov-Smirnov Z .993 1.834 2.248 Asymp. Sig. (2-tailed) .277 .002 .000 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  TPC Levene Statistic df1 df2 Sig.

  22.393

  6 56 .000

  

ANOVA

  TPC Sum of Squares df Mean Square F Sig. Between Groups 367.064 6 61.177 22.592 .000 Within Groups 151.647 56 2.708 Total 518.711

  62 Robust Tests of Equality of Means TPC

  Statistic

  a df1 df2 Sig.

  Brown-Forsythe 22.592 6 14.732 .000 a. Asymptotically F distributed.

  Post Hoc Tests Multiple Comparisons

  TPC Games-Howell (I)

  ISOLAT (J)

  ISOLAT Mean Difference (I-J) Std. Error Sig.

  95% Confidence Interval Lower Bound Upper Bound

  OA2E1 SA1A .27778 .36772 .986 -.9888 1.5444 OA2G .32222 .58161 .997 -1.6717 2.3162 OA2E2 .46667 .34721 .819 -.7598 1.6931 MB1C -.10000 .36132 1.000 -1.3528 1.1528 SA2A -.02222 .50464 1.000 -1.7250 1.6805 OA1E 7.03333

• 1.27279 .004 2.3174 11.7493

  SA1A OA2E1 -.27778 .36772 .986 -1.5444 .9888 OA2G .04444 .52561 1.000 -1.8453 1.9341 OA2E2 .18889 .24197 .984 -.6288 1.0066 MB1C -.37778 .26182 .772 -1.2560 .5004

  SA2A -.30000 .43892 .991 -1.8464 1.2464 OA1E 6.75556

• 1.24820 .006 2.0472 11.4639

  OA2G OA2E1 -.32222 .58161 .997 -2.3162 1.6717 SA1A -.04444 .52561 1.000 -1.9341 1.8453 OA2E2 .14444 .51147 1.000 -1.7313 2.0202 MB1C -.42222 .52116 .978 -2.3068 1.4624 SA2A -.34444 .62905 .997 -2.4653 1.7764 OA1E 6.71111

• 1.32705 .005 1.9466 11.4756

  OA2E2 OA2E1 -.46667 .34721 .819 -1.6931 .7598 SA1A -.18889 .24197 .984 -1.0066 .6288 OA2G -.14444 .51147 1.000 -2.0202 1.7313 MB1C -.56667 .23214 .246 -1.3483 .2150 SA2A -.48889 .42189 .895 -2.0111 1.0333 OA1E 6.56667

• 1.24231 .008 1.8586 11.2748

  MB1C OA2E1 .10000 .36132 1.000 -1.1528 1.3528 SA1A .37778 .26182 .772 -.5004 1.2560 OA2G .42222 .52116 .978 -1.4624 2.3068 OA2E2 .56667 .23214 .246 -.2150 1.3483 SA2A .07778 .43358 1.000 -1.4600 1.6156 OA1E 7.13333

• 1.24633 .004 2.4251 11.8415

  SA2A OA2E1 .02222 .50464 1.000 -1.6805 1.7250 SA1A .30000 .43892 .991 -1.2464 1.8464 OA2G .34444 .62905 .997 -1.7764 2.4653 OA2E2 .48889 .42189 .895 -1.0333 2.0111 MB1C -.07778 .43358 1.000 -1.6156 1.4600 OA1E 7.05556

• 1.29516 .004 2.3244 11.7867
• 1.27279 .004 -11.7493 -2.3174

  OA1E OA2E1 -7.03333

• 1.24820 .006 -11.4639 -2.0472

  SA1A -6.75556

• 1.32705 .005 -11.4756 -1.9466

  OA2G -6.71111

• OA2E2 -6.56667 1.24231 .008 -11.2748 -1.8586
• MB1C -7.13333 1.24633 .004 -11.8415 -2.4251
• SA2A -7.05556 1.29516 .004 -11.7867 -2.3244 *. The mean difference is significant at the 0.05 level.

b. Data pH media kultur dengan senyawa hidrokarbon alifatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU pH N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 6.7905 Std. Deviation 2.016 2.89047 .32165

  Most Extreme Differences Absolute .125 .231 .298 Positive .125 .213 .173 Negative -.125 -.231 -.298

  Kolmogorov-Smirnov Z .993 1.834 2.367 Asymp. Sig. (2-tailed) .277 .002 .000 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  pH Levene Statistic df1 df2 Sig.

  .612

  6 56 .720

  

ANOVA

  pH Sum of Squares df Mean Square F Sig. Between Groups .065 6 .011 .096 .996 Within Groups 6.349 56 .113 Total 6.414

  62 Post Hoc Tests

  Homogeneous Subsets pH

  Duncan Subset for alpha

  = 0.05

  ISOLAT N

  1 OA2E1 9 6.7444 OA1E 9 6.7556 SA1A 9 6.7889 OA2E2 9 6.7889 SA2A 9 6.7889 OA2G 9 6.8222 MB1C 9 6.8444 Sig. .594 Means for groups in homogeneous subsets are displayed.

c. Data TPC bakteri pada senyawa hidrokarbon monoaromatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU TPC N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 8.7587 Std. Deviation 2.016 2.89047 1.65155

  Most Extreme Differences Absolute .125 .231 .121 Positive .125 .213 .083 Negative -.125 -.231 -.121

  Kolmogorov-Smirnov Z .993 1.834 .958 Asymp. Sig. (2-tailed) .277 .002 .317 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  TPC Levene Statistic df1 df2 Sig.

  1.576

  6 56 .171

  

ANOVA

  TPC Sum of Squares df Mean Square F Sig. Between Groups 20.722 6 3.454 1.303 .271 Within Groups 148.391 56 2.650 Total 169.113

  62

  Post Hoc Tests Homogeneous Subsets TPC

  Duncan Subset for alpha = 0.05

  ISOLAT N

  1

  2 OA2E1 9 7.7333 MB1C 9 8.2778 8.2778 OA2E2 9 8.6444 8.6444 OA1E 9 8.8667 8.8667 SA1A 9 8.9889 8.9889 SA2A 9 9.1556 9.1556 OA2G 9 9.6444 Sig. .110 .125 Means for groups in homogeneous subsets are displayed.

d. Data pH pada media kultur dengan senyawa hidrokarbon monoaromatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU pH N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 6.8238 Std. Deviation 2.016 2.89047 .13879

  Most Extreme Differences Absolute .125 .231 .231 Positive .125 .213 .219 Negative -.125 -.231 -.231

  Kolmogorov-Smirnov Z .993 1.834 1.835

  Asymp. Sig. (2-tailed) .277 .002 .002 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  pH Levene Statistic df1 df2 Sig.

  .115

  6 56 .994

  

ANOVA

  pH Sum of Squares df Mean Square F Sig. Between Groups .012 6 .002 .095 .997 Within Groups 1.182 56 .021 Total 1.194

  62 Post Hoc Tests

  Homogeneous Subsets pH

  Duncan Subset for alpha

  = 0.05

  ISOLAT N

  1 OA2E2 9 6.8000 SA2A 9 6.8111 MB1C 9 6.8222 OA1E 9 6.8222 SA1A 9 6.8333 OA2G 9 6.8333

  OA2E1 9 6.8444 Sig. .583 Means for groups in homogeneous subsets are displayed.

e. Data TPC bakteri pada senyawa hidrokarbon poliaromatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU TPC N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 10.6111 Std. Deviation 2.016 2.89047 2.73697

  Most Extreme Differences Absolute .125 .231 .152 Positive .125 .213 .119 Negative -.125 -.231 -.152

  Kolmogorov-Smirnov Z .993 1.834 1.205 Asymp. Sig. (2-tailed) .277 .002 .110 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  TPC Levene Statistic df1 df2 Sig.

  .428

  6 56 .857

  

ANOVA

  TPC Sum of Squares df Mean Square F Sig. Between Groups 21.251 6 3.542 .448 .844 Within Groups 443.191 56 7.914 Total 464.442

  62 Homogeneous Subsets

  TPC

  Duncan Subset for alpha

  = 0.05

  ISOLAT N

  1 OA2E2 9 9.6444 OA2G 9 10.2222 SA1A 9 10.4000 OA1E 9 10.4444 OA2E1 9 10.8222 SA2A 9 11.3667 MB1C 9 11.3778 Sig. .268 Means for groups in homogeneous subsets are displayed.

f. Data pH pada media kultur dengan senyawa hidrokarbon poliaromatik NPar Tests

  

One-Sample Kolmogorov-Smirnov Test

  ISOLAT WAKTU pH N

  63

  63

  63

  a

  Normal Parameters Mean 4.00 3.3333 6.8254 Std. Deviation 2.016 2.89047 .16458

  Most Extreme Differences Absolute .125 .231 .237 Positive .125 .213 .158 Negative -.125 -.231 -.237

  Kolmogorov-Smirnov Z .993 1.834 1.878 Asymp. Sig. (2-tailed) .277 .002 .002 a. Test distribution is Normal.

  Oneway Test of Homogeneity of Variances

  pH Levene Statistic df1 df2 Sig.

  .085

  6 56 .997

  

ANOVA

  pH Sum of Squares df Mean Square F Sig. Between Groups .039 6 .007 .224 .967 Within Groups 1.640 56 .029 Total 1.679

  62 Post Hoc Tests

  Homogeneous Subsets pH

  Duncan Subset for alpha

  = 0.05

  ISOLAT N

  1 OA2E1 9 6.7889 SA1A 9 6.8000 OA1E 9 6.8111 OA2G 9 6.8333 OA2E2 9 6.8333 MB1C 9 6.8444 SA2A 9 6.8667 Sig. .414 Means for groups in homogeneous subsets are displayed.

  Lampiran 2 Hasil analisis GC

a. Hasil analisis standar baku 1000 ppm (n-heksadekana, fenol, dan fenantren)

b. Hasil analisis standar baku 750 ppm

c. Hasil analisis standar baku 500 ppm

  d. Kalibrasi n-heksadekana

  e. Kalibrasi fenol

f. Kalibrasi fenantren g.

  

Hasil analisis GC senyawa n-heksadekana pada sampel hari ke-0

h. Hasil analisis GC senyawa n-heksadekana pada sampel hari ke-7

i. Hasil analisis GC senyawa fenol pada sampel hari ke-0

  j. Hasil analisis GC senyawa fenol pada sampel hari ke-7

  k. Hasil analisis GC senyawa fenantren pada sampel hari ke-0

  l. Hasil analisis GC senyawa fenantren pada sampel hari ke-7

  Lampiran 3 Perhitungan persentase degradasi

  a. N-heksadekana  y= 0,043x + 520,2

  Faktor kalibrasi  x= 4924, y= 0,043 . 4924 + 520,2

  Luas area hari ke-0 = 731,932 ppm  x= 4659, y= 0,043 . 4659 + 520,2

  Luas area hari ke-7 = 720,537 ppm

  b. Fenol  y= 0,843x + 210,3

  Faktor kalibrasi  x= 630, y= 0,843 . 630 + 210,3

  Luas area hari ke-0 = 741,39 ppm Luas area hari ke-7  x= 560, y= 0,843 . 560 + 210,3 = 682,38 ppm c. Fenantren Faktor kalibrasi  y= 0,038x + 532  x= 12224, y= 0,038 . 12224 + 532

  Luas area hari ke-0 = 996,512 ppm  x= 8152, y= 0,038 . 8152 + 532

  Luas area hari ke-7 = 841,776 ppm

  Lampiran 4 Hasil uji kit dan uji fisiologis

a. SA2A

  1 Lysine kuning (-)

  17 Rhamnose hijau (-)

  ada gelembung udara

  2 O 2 tidak berwarna (+)

  27 H

  26 Nitrat (+Zn)

kuning (+)

  25 Nitrat kuning (-)

  24 Arginine kuning (-)

  23 Salicin biru - hijau (-)

  22 Raffinose biru - hijau (-)

  21 Adonitol biru - hijau (-)

  20 Arabinose kuning (+)

  19 Lactose biru - hijau (-)

  18 Sucrose biru - hijau (-)

  16 Sorbitol biru - hijau (-)

  2 Ornithine kuning (-)

  No. Substrat Warna Hasil Keterangan

  14 Malonate kuning (-)

  13 Gelatin tidak berwarna (-)

  12 TDA kuning (-)

  11 Citrate kuning (-)

  10 VP merah ceri (+/-)

  9 Urease merah muda (+)

  8 Indole tidak berwarna (-)

  7 ONPG tidak berwarna (-)

  6 Xylose kuning (+)

  5 Mannitol biru - hijau (-)

  4 Glucose hijau (-)

  3 H2S warna jerami (-)

  15 Inositol biru - hijau (-)

b. OA2G

  17 Rhamnose biru - hijau (-)

  tidak berwarna (+) ada gelembung udara

  2

  2 O

  27 H

  26 Nitrat (+Zn) tidak dilakukan

  25 Nitrat merah ceri (+)

  24 Arginine kuning- hijau (+)

  23 Salicin biru - hijau (-)

  22 Raffinose biru - hijau (-)

  21 Adonitol biru - hijau (-)

  20 Arabinose kuning (+)

  19 Lactose biru - hijau (-)

  18 Sucrose biru - hijau (-)

  No. Substrat Warna Hasil Keterangan

  1 Lysine kuning - hijau (-)

  15 Inositol biru - hijau (-)

  14 Malonate kuning (-)

  13 Gelatin tidak berwarna (-)

  12 TDA kuning (-)

  11 Citrate Hijau (-)

  10 VP Merah ceri (+/-)

  9 Urease warna jerami (-)

  8 Indole tidak berwarna (-)

  7 ONPG tidak berwarna (-)

  6 Xylose kuning tua (+)

  5 Mannitol biru - hijau (-)

  4 Glucose biru-hijau (-)

  3 H2S warna jerami (-)

  2 Ornithine kuning - hijau (-)

  16 Sorbitol biru - hijau (-)

a. Isolat bakteri SA2A pada uji amilase positif

  6

  19

  18

  16

  14

  12

  11

  10

  9

  7

  5

  24

  4

  3

  2

  1

  2A

  No Jenis Uji SA

  1 Pewarnaan gram + + + + + d + + + + + d d d - - d d

  2 Bentuk sel bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt

  3 Sel yang membentuk rantai + + + + + d d + d d d d d d - - d d

  Tabel persamaan karakteristik

  22

• d d d - -
• d - - -

  megaterium ;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16) B.circulans; (18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

  39

  18 Urease + - d d - - + d - d - - d - - - - -

  19 Indole - - - - - - - - - - - - + - - - - -

  20 VP (Voges Proskauer) d + + + + - - - + d + d + d d + d d

  21 Reduksi nitrate - + + + + + - d + + + d - d + + + d

  22 Oksidase + d d d d - + - - - - - + - + - - -

  23 Hidrolisis kasein (Protein) + + + + + + - + + + + + + - - + - d

  24 Hidrolisis tepung (Amilum) + + + + + +

  72

  71

  54

  60

  40

  16 ONPG - - - d - d + + + + d d d + + + d -

  50

  57

  68

  48

  56

  56

  65

  62

  48

  55

  60

  17 Citrate - - d d + - - + + + d d - - - d - -

  15 Xylose + - - - - - - + d + d d - + + + d -

  (1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B.

  X V

  Keterangan: (T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

  4 Motilitas + - + - + + + + + + +

  5 Panjang sel > 3 µ m +

  6 Bentuk dan ukuran spora

  VX VX

  VX VX

  VX VX

  VX VX

  VX VX

  V X

  V T

  X V T

  14 Salicin - - + d d - d + + + + d d + + + d d

  X V

  X V

  X VT

  X

  7 Pembengkakan sel spora -

  d d - - - - d + + + + d + Uji produksi asam dari fermentasi karbohidrat

  10 Glucose - +

  11 Galactose (Gelatin) - - - d - - d + d + d d d + + + - -

  12 Mannose (Manitol) - - - - d d + d + + d + d + + + + +

  13 Raffinose - - - - - - - d + d + + + + + + d +

  X V

• Koefisien sebanding (%)

b. Isolat bakteri OA2G pada uji amilase positif

  6

  19

  18

  16

  14

  12

  11

  10

  9

  7

  5

  24

  4

  3

  2

  1

  2G

  No Jenis Uji OA

  1 Pewarnaan gram + + + + + d + + + + + d d d - - d d

  2 Bentuk sel bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt

  3 Sel yang membentuk rantai - + + + + d d + d d d d d d - - d d

  22

• d d d - -
• d - - -

  megaterium ;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16) B.circulans; (18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. stearothermophilus

  36

  18 Urease - - d d - - + d - d - - d - - - - -

  19 Indole - - - - - - - - - - - - + - - - - -

  20 VP (Voges Proskauer) d + + + + - - - + d + d + d d + d d

  21 Reduksi nitrate + + + + + + - d + + + d - d + + + d

  22 Oksidase + d d d d - + - - - - - + - + - - -

  23 Hidrolisis kasein (Protein) + + + + + + - + + + + + + - - + - d

  25 Hidrolisis tepung (Amilum) + + + + + +

  70

  58

  43

  58

  25

  16 ONPG - - - d - d + + + + d d d + + + d -

  38

  55

  57

  47

  57

  38

  60

  61

  46

  55

  65

  17 Citrate - - d d + - - + + + d d - - - d - -

  15 Xylose + - - - - - - + d + d d - + + + d -

  (1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B.

  X V

  Keterangan: (T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

  4 Motilitas + - + - + + + + + + +

  5 Panjang sel > 3 µ m -

  6 Bentuk dan ukuran spora

  VX VX

  VX VX

  VX VX

  VX VX

  VX VX

  V X

  V T

  X V T

  14 Salicin - - + d d - d + + + + d d + + + d d

  X V

  X V

  X VT

  X

  7 Pembengkakan sel spora -

  d d - - - - d + + + + d + Uji produksi asam dari fermentasi karbohidrat

  10 Glucose - +

  11 Galactose (Gelatin) - - - d - - d + d + d d d + + + - -

  12 Mannose (Manitol) - - - - d d + d + + d + d + + + + +

  13 Raffinose - - - - - - - d + d + + + + + + d +

  X V

• Koefisien sebanding (%)

  Lampiran 5 Dokumentasi

a. Alat dan bahan Keterangan :

  a= colony counter d= spektrofotometer g= shaker b= autoclave e= inkubator h= kompor listrik c= timbangan analitik f= lemari es

  Keterangan :

  1= kapas 11= tabung reaksi 2= korek api 12= pipetor 3= sendok media 13= hand counter 4= cawan petri 14= botol kultur 5= tip 1000µL 15= gelas ukur 10 mL 6= tip 100 µL 16= bunsen 7= spatula besi 17= botol aquades 8= mikropipet 18= beaker glass 9= pipet ukur 19= masker 10= ose 20= alumunium foil 21= gelas ukur 500 mL 22= labu pisah Senyawa murni hidrokarbon: 1= fenantren 2= n-heksadekana

3= fenol

b. Isolat bakteri

  c. Media kultur n-heksadekana (hari ke-3 dan hari ke-7) Hari ke-3 Hari ke-7

  Lanjut Gambar Media Kultur Fenol (hari ke-3 dan hari ke-7) Hari ke-3 Hari ke-7

  Fenantren (hari ke-3 dan hari ke-7) Hari ke-7 Hari ke-3

d. Hasil TPC

MB1C OA1E

  Lanjutan Gambar Hasil TPC e. Hasil uji fisiologi bakteri menggunakan microbact identification kit OA2E1 OA2E2 OA2G SA1A SA2A

  f. Hasil uji oksidase Keterangan: Warna kertas berubah = positif g. Hasil uji amilolitik h. Hasil uji proteolitik i.

  2 O

  2 Hasil uji H Gelembung udara

• d d d - -
• d - - -
• Koefisien sebanding (%)

  VX VT

  VX VX

  VX VX

  VX VX

  VX VX

  VX VX

  VX VTX

  VX VTX

  VX VX

  X

  5 Panjang sel > 3 µm +

  7 Pembengka kan sel spora -

  d d - - - - d + + + + d + Uji produksi asam dari fermentasi karbohidrat

  10 Glucose - +

  11 Galactose (Gelatin) - - - d - - d + d + d d d + + + - -

  12 Mannose (Manitol) - - - - d d + d + + d + d + + + + +

  13 Raffinose - - - - - - - d + d + + + + + + d +

  14 Salicin - - + d d - d + + + + d d + + + d d

  15 Xylose + - - - - - - + d + d d - + + + d -

  Skripsi Deteksi Kemampuan Degradasi Hidrokarbon

Alifatik dan Aromatik oleh Isolat Bakteri Hidrokarbonoklastik dari Lumpur

Pantai Kenjeran

  6 Bentuk dan ukuran spora

  4 Motilitas + - + - + + + + + + +

  Tabel persamaan karakteristik a. Isolat bakteri SA2A pada uji amilase positif

  10

  No Jenis Uji SA2A

  1

  2

  3

  4

  5

  6

  7

  9

  11

  3 Sel yang membentuk rantai + + + + + d d + d d d d d d - - d d

  12

  14

  16

  18

  19

  22

  24

  1 Pewarnaan gram + + + + + d + + + + + d d d - - d d

  2 Bentuk sel bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt

  Walliyana Kusumaningati

  Keterangan: (T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

  39

  60 Skripsi Deteksi Kemampuan Degradasi Hidrokarbon

Alifatik dan Aromatik oleh Isolat Bakteri Hidrokarbonoklastik dari Lumpur

Pantai Kenjeran

  55

  48

  62

  65

  56

  56

  48

  68

  57

  50

  40

  60

  

(1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B.

megaterium ;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16)

B.circulans; (18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

  54

  71

  72

  24 Hidrolisis tepung (Amilum) + + + + + +

  23 Hidrolisis kasein (Protein) + + + + + + - + + + + + + - - + - d

  22 Oksidase + d d d d - + - - - - - + - + - - -

  21 Reduksi nitrate - + + + + + - d + + + d - d + + + d

  

Proskauer ) d + + + + - - - + d + d + d d + d d

  20 VP (Voges

  19 Indole - - - - - - - - - - - - + - - - - -

  18 Urease + - d d - - + d - d - - d - - - - -

  17 Citrate - - d d + - - + + + d d - - - d - -

  16 ONPG - - - d - d + + + + d d d + + + d -

  Walliyana Kusumaningati

b. Isolat bakteri OA2G pada uji amilase positif

  10

  24

  22

  19

  18

  16

  14

  12

  11

  9

  2 Bentuk sel bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt bt

  7

  6

  5

  4

  3

  2

  1

  No Jenis Uji OA2G

  3 Sel yang membentuk rantai - + + + + d d + d d d d d d - - d d

  1 Pewarnaan gram + + + + + d + + + + + d d d - - d d

• d d d - -
• d - - -

  6 Bentuk dan ukuran spora

  7 Pembengkakan sel spora -

  Walliyana Kusumaningati

  Skripsi Deteksi Kemampuan Degradasi Hidrokarbon

Alifatik dan Aromatik oleh Isolat Bakteri Hidrokarbonoklastik dari Lumpur

Pantai Kenjeran

  17 Citrate - - d d + - - + + + d d - - - d - -

  16 ONPG - - - d - d + + + + d d d + + + d -

  15 Xylose + - - - - - - + d + d d - + + + d -

  14 Salicin - - + d d - d + + + + d d + + + d d

  13 Raffinose - - - - - - - d + d + + + + + + d +

  12 Mannose (Manitol) - - - - d d + d + + d + d + + + + +

  11 Galactose (Gelatin) - - - d - - d + d + d d d + + + - -

  10 Glucose - +

  d d - - - - d + + + + d + Uji produksi asam dari fermentasi karbohidrat

  VT

  X

  4 Motilitas + - + - + + + + + + +

  VX VX

  X VX

  VT

  X VX

  VX VT

  VX VX

  VX VX

  VX VX

  VX VX

  VX VX

  5 Panjang sel > 3 µ m -

• Koefisien sebanding (%)

  Keterangan: (T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

  70

  55 57 47 57 38 60 61 46 55 65 Skripsi Deteksi Kemampuan Degradasi Hidrokarbon

  38

  25

  36

  58

  43

  58

  25 Hidrolisis tepung (Amilum) + + + + + +

  

(1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B.

megaterium ;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16)

B.circulans; (18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

  23 Hidrolisis kasein (Protein) + + + + + + - + + + + + + - - + - d

  22 Oksidase + d d d d - + - - - - - + - + - - -

  21 Reduksi nitrate + + + + + + - d + + + d - d + + + d

  Proskauer ) d + + + + - - - + d + d + d d + d d

  20 VP (Voges

  19 Indole - - - - - - - - - - - - + - - - - -

  18 Urease - - d d - - + d - d - - d - - - - -

  

Alifatik dan Aromatik oleh Isolat Bakteri Hidrokarbonoklastik dari Lumpur

Pantai Kenjeran Walliyana Kusumaningati