jsfk1,2,rika yulia Abstract 2015

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Jurnal Sains Farmasi & Klinis, 1(2), 122-131

ARTIKEL PENELITIAN

An Assay of Antioxidant Power of Methanolic Extract Various
Type of Soybean
Rika Yulia, Azminah, Michella & Andre Tanzil

Keywords:
Soybean (Glycine

ABSTRACT: This study aimed to examine the antioxidant activity of methanolic
extract of various type of soybean (Glycine max L.Merill) i.e

Argomulyo,

max L.Merill),

Burangrang, Ijen, and Kaba by using DPPH (1,1-Diphenyl-2-picryhydrazyl)


Argomulyo variety,

method. The soybean was crushed, defatted using n-hexan, and extracted using

Burangrang

methanol 90%. The processes of defatting and extracting were conducted by

variety, Ijen

kinesthetic maceration. Identiication of lavonoid content using KLT and an

variety,

assay of the antioxidant power of soybean were carried out qualitatively and

Kaba variety,

quantitavely. Qualitative analysis, the color of DPPH solution was fading from


antioxidant

violet into yellowish. Quantitative analysis showed that the maximum wave-

power, DPPH

length of DPPH in methanol was 516,00 nm within 15-minute reaction time.

(1,1-Diphenyl-2-

The effective concentration 50% (EC50) of each extract was alsa determined.

picryhydrazyl)

Results of this study revealed that the methanolic extract of soybean taken from
varieties of Argomulyo, Burangrang, Ijen, and Kaba contained lavonoid, with
EC50 value of each variety subsequently ranging from 3620.22 bpj; 5290.71 bpj;
4145.99 bpj; and 4253.50 bpj. Argomulyo variety showed the highest antioxidant
power.


Faculty of Pharmacy, University of Surabaya
Korespondensi:
Rika Yulia
(rika.y73@gmail.com)

122

Jurnal Sains Farmasi & Klinis (ISSN: 2407-7062) | Vol. 01 No. 02 | Mei 2015