Tampilan Imunohistokimia Epidermalgrowth Factor Reseptor (Egfr) Dan B-Cell Leukemia Lymphoma-2 (Bcl-2) Pada Subtipe Histopatologi Karsinoma Sel Basal
Universitas Sumatera Utara
Lampiran 2
No. sample
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
DX
2
4
3
2
2
3
3
3
3
2
2
3
4
3
3
3
2
6
2
1
4
3
1
3
3
3
3
2
4
5
3
2
2
5
3
3
2
2
5
5
EGFR
+3
0
+3
0
+1
0
0
0
0
+2
+3
+1
+3
+1
+1
+1
+3
0
0
0
+3
+2
+2
+3
+3
+2
+3
+3
+1
+3
+1
+3
+3
0
+1
+1
+3
+1
+3
+3
EGFR Status
2
1
2
1
1
1
1
1
1
2
2
1
2
1
1
1
2
1
1
1
2
2
2
2
2
2
2
2
1
2
1
2
2
1
1
1
2
1
2
2
BCL-2
+4
0
+3
0
+1
+2
+1
+1
+3
0
+2
+4
+3
+4
+2
+3
+4
+1
+2
+3
+1
+4
+1
+1
+4
+4
0
+4
+3
+4
+3
+3
+3
0
+3
+2
+3
+1
0
+2
BCL-2 Status
2
1
2
1
1
1
1
1
2
1
1
2
2
2
1
2
2
1
1
2
1
2
1
1
2
2
1
2
2
2
2
2
2
1
2
1
2
1
1
1
Keterangan
Diagnosa
1. Superfisial Tipe
2. Nodular Tipe
3. Mikro Nodular Tipe
4. Infiltrating Tipe
5. Pigmented Tipe
6. Basosquamous Tipe
EGFR
Status
1. Lemah
2. Kuat
(0-1)
(2-3)
BCL-2
1. Lemah
2. Kuat
(0-2)
(3-4)
Universitas Sumatera Utara
LAMPIRAN 3
. tab dx
Diagnosis
Freq.
Percent
Cum.
superficial
nodular
mikro-nodular
infiltrating
pigmented
Basosquamous
2
12
17
4
4
1
5.00
30.00
42.50
10.00
10.00
2.50
5.00
35.00
77.50
87.50
97.50
100.00
Total
40
100.00
. tab egfr
nilai egfr
Freq.
Percent
Cum.
negatif
+1
+2
+3
10
10
4
16
25.00
25.00
10.00
40.00
25.00
50.00
60.00
100.00
Total
40
100.00
nilai BCL 2
positif
Freq.
Percent
Cum.
negatif
+1
+2
+3
+4
6
8
6
11
9
15.00
20.00
15.00
27.50
22.50
15.00
35.00
50.00
77.50
100.00
Total
40
100.00
status egfr
kuat/lemah
Freq.
Percent
Cum.
lemah
kuat
20
20
50.00
50.00
50.00
100.00
Total
40
100.00
status bcl2
kuat/lemah
Freq.
Percent
Cum.
lemah
kuat
20
20
50.00
50.00
50.00
100.00
Total
40
100.00
. tab bcl2
. tab statusegfr
. tab statusbcl2
Universitas Sumatera Utara
. tab dx statusegfr, row
Key
frequency
row percentage
Diagnosis
status egfr
kuat/lemah
lemah
kuat
Total
superficial
1
50.00
1
50.00
2
100.00
nodular
4
33.33
8
66.67
12
100.00
mikro-nodular
11
64.71
6
35.29
17
100.00
infiltrating
2
50.00
2
50.00
4
100.00
pigmented
1
25.00
3
75.00
4
100.00
Basosquamous
1
100.00
0
0.00
1
100.00
Total
20
50.00
20
50.00
40
100.00
. tab dx statusbcl2, row
Key
frequency
row percentage
Diagnosis
status bcl2
kuat/lemah
lemah
kuat
Total
superficial
1
50.00
1
50.00
2
100.00
nodular
6
50.00
6
50.00
12
100.00
mikro-nodular
7
41.18
10
58.82
17
100.00
infiltrating
2
50.00
2
50.00
4
100.00
pigmented
3
75.00
1
25.00
4
100.00
Basosquamous
1
100.00
0
0.00
1
100.00
Total
20
50.00
20
50.00
40
100.00
Universitas Sumatera Utara
. mcc statusegfr statusbcl2
Controls
Exposed
Unexposed
Total
Exposed
Unexposed
12
8
8
12
20
20
Total
20
20
40
Cases
McNemar's chi2(1) =
0.00
Prob > chi2 = 1.0000
Exact McNemar significance probability
= 1.0000
Proportion with factor
Cases
Controls
.5
.5
[95% Conf. Interval]
difference
ratio
rel. diff.
0
1
0
-.2209964
.675709
-.3919928
.2209964
1.479927
.3919928
odds ratio
1
.3271578
3.056629
(exact)
. tab statusegfr statusbcl2, col chi exp exact
Key
frequency
expected frequency
column percentage
status
egfr
kuat/lemah
status bcl2
kuat/lemah
lemah
kuat
Total
lemah
12
10.0
60.00
8
10.0
40.00
20
20.0
50.00
kuat
8
10.0
40.00
12
10.0
60.00
20
20.0
50.00
Total
20
20.0
100.00
20
20.0
100.00
40
40.0
100.00
Pearson chi2(1) =
Fisher's exact =
1-sided Fisher's exact =
1.6000
Pr = 0.206
0.343
0.172
Universitas Sumatera Utara
Lampiran 4
A
C
E
B
D
F
Gambara 1. KSB dengan pewarnaan Hematoksilin & Eosin 100x, (A) subtipe superfisial, (B)
subtipe nodular (C) subtipe infiltrating, (D subtipe mikro-nodular), (E) suntipe basoskuamous, (F)
piqmented
Universitas Sumatera Utara
A
B
Gambar 3. Dengan pewarnaan imunohistokimia BCL-2
dengan Pembesaran 400x (A) ekspresi kuat (B) ekspresi
lemah dan (C) ekspresi negatif
C
A
B
Gambar 3. dengan pewarnaan imunohistokimia EGFR
dengan Pembesaran 400x (A) ekspresi kuat (B) ekspresi
lemah dan (C) ekspresi negatif
C
Universitas Sumatera Utara
Lampiran 2
No. sample
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
DX
2
4
3
2
2
3
3
3
3
2
2
3
4
3
3
3
2
6
2
1
4
3
1
3
3
3
3
2
4
5
3
2
2
5
3
3
2
2
5
5
EGFR
+3
0
+3
0
+1
0
0
0
0
+2
+3
+1
+3
+1
+1
+1
+3
0
0
0
+3
+2
+2
+3
+3
+2
+3
+3
+1
+3
+1
+3
+3
0
+1
+1
+3
+1
+3
+3
EGFR Status
2
1
2
1
1
1
1
1
1
2
2
1
2
1
1
1
2
1
1
1
2
2
2
2
2
2
2
2
1
2
1
2
2
1
1
1
2
1
2
2
BCL-2
+4
0
+3
0
+1
+2
+1
+1
+3
0
+2
+4
+3
+4
+2
+3
+4
+1
+2
+3
+1
+4
+1
+1
+4
+4
0
+4
+3
+4
+3
+3
+3
0
+3
+2
+3
+1
0
+2
BCL-2 Status
2
1
2
1
1
1
1
1
2
1
1
2
2
2
1
2
2
1
1
2
1
2
1
1
2
2
1
2
2
2
2
2
2
1
2
1
2
1
1
1
Keterangan
Diagnosa
1. Superfisial Tipe
2. Nodular Tipe
3. Mikro Nodular Tipe
4. Infiltrating Tipe
5. Pigmented Tipe
6. Basosquamous Tipe
EGFR
Status
1. Lemah
2. Kuat
(0-1)
(2-3)
BCL-2
1. Lemah
2. Kuat
(0-2)
(3-4)
Universitas Sumatera Utara
LAMPIRAN 3
. tab dx
Diagnosis
Freq.
Percent
Cum.
superficial
nodular
mikro-nodular
infiltrating
pigmented
Basosquamous
2
12
17
4
4
1
5.00
30.00
42.50
10.00
10.00
2.50
5.00
35.00
77.50
87.50
97.50
100.00
Total
40
100.00
. tab egfr
nilai egfr
Freq.
Percent
Cum.
negatif
+1
+2
+3
10
10
4
16
25.00
25.00
10.00
40.00
25.00
50.00
60.00
100.00
Total
40
100.00
nilai BCL 2
positif
Freq.
Percent
Cum.
negatif
+1
+2
+3
+4
6
8
6
11
9
15.00
20.00
15.00
27.50
22.50
15.00
35.00
50.00
77.50
100.00
Total
40
100.00
status egfr
kuat/lemah
Freq.
Percent
Cum.
lemah
kuat
20
20
50.00
50.00
50.00
100.00
Total
40
100.00
status bcl2
kuat/lemah
Freq.
Percent
Cum.
lemah
kuat
20
20
50.00
50.00
50.00
100.00
Total
40
100.00
. tab bcl2
. tab statusegfr
. tab statusbcl2
Universitas Sumatera Utara
. tab dx statusegfr, row
Key
frequency
row percentage
Diagnosis
status egfr
kuat/lemah
lemah
kuat
Total
superficial
1
50.00
1
50.00
2
100.00
nodular
4
33.33
8
66.67
12
100.00
mikro-nodular
11
64.71
6
35.29
17
100.00
infiltrating
2
50.00
2
50.00
4
100.00
pigmented
1
25.00
3
75.00
4
100.00
Basosquamous
1
100.00
0
0.00
1
100.00
Total
20
50.00
20
50.00
40
100.00
. tab dx statusbcl2, row
Key
frequency
row percentage
Diagnosis
status bcl2
kuat/lemah
lemah
kuat
Total
superficial
1
50.00
1
50.00
2
100.00
nodular
6
50.00
6
50.00
12
100.00
mikro-nodular
7
41.18
10
58.82
17
100.00
infiltrating
2
50.00
2
50.00
4
100.00
pigmented
3
75.00
1
25.00
4
100.00
Basosquamous
1
100.00
0
0.00
1
100.00
Total
20
50.00
20
50.00
40
100.00
Universitas Sumatera Utara
. mcc statusegfr statusbcl2
Controls
Exposed
Unexposed
Total
Exposed
Unexposed
12
8
8
12
20
20
Total
20
20
40
Cases
McNemar's chi2(1) =
0.00
Prob > chi2 = 1.0000
Exact McNemar significance probability
= 1.0000
Proportion with factor
Cases
Controls
.5
.5
[95% Conf. Interval]
difference
ratio
rel. diff.
0
1
0
-.2209964
.675709
-.3919928
.2209964
1.479927
.3919928
odds ratio
1
.3271578
3.056629
(exact)
. tab statusegfr statusbcl2, col chi exp exact
Key
frequency
expected frequency
column percentage
status
egfr
kuat/lemah
status bcl2
kuat/lemah
lemah
kuat
Total
lemah
12
10.0
60.00
8
10.0
40.00
20
20.0
50.00
kuat
8
10.0
40.00
12
10.0
60.00
20
20.0
50.00
Total
20
20.0
100.00
20
20.0
100.00
40
40.0
100.00
Pearson chi2(1) =
Fisher's exact =
1-sided Fisher's exact =
1.6000
Pr = 0.206
0.343
0.172
Universitas Sumatera Utara
Lampiran 4
A
C
E
B
D
F
Gambara 1. KSB dengan pewarnaan Hematoksilin & Eosin 100x, (A) subtipe superfisial, (B)
subtipe nodular (C) subtipe infiltrating, (D subtipe mikro-nodular), (E) suntipe basoskuamous, (F)
piqmented
Universitas Sumatera Utara
A
B
Gambar 3. Dengan pewarnaan imunohistokimia BCL-2
dengan Pembesaran 400x (A) ekspresi kuat (B) ekspresi
lemah dan (C) ekspresi negatif
C
A
B
Gambar 3. dengan pewarnaan imunohistokimia EGFR
dengan Pembesaran 400x (A) ekspresi kuat (B) ekspresi
lemah dan (C) ekspresi negatif
C
Universitas Sumatera Utara