Exogenous methyl jasmonate prevents necr Abstract 2015
Plant Cell Tiss Organ Cult
DOI 10.1007/s11240-015-0838-8
ORIGINAL ARTICLE
Exogenous methyl jasmonate prevents necrosis caused
by mechanical wounding and increases terpenoid biosynthesis
in Panax ginseng
Sri Renuka Devi Balusamy1,2 • Shadi Rahimi2 • Johan Sukweenadhi2
Yu-Jin Kim2 • Deok-Chun Yang2,3
•
Received: 25 September 2014 / Accepted: 31 July 2015
Ó Springer Science+Business Media Dordrecht 2015
Abstract Upon mechanical wounding, plants locally
induce necrosis, accumulate methyl jasmonate (MeJA) and
acquire systemic resistance in nearby tissues. One-monthold in vitro grown Panax ginseng seedlings were treated
with either 50 lM MeJA or mechanical wounding alone or
a combination of both, to evaluate jasmonic acid (JA)
signaling and terpene biosynthetic pathway genes along
with terpenoid accumulation. After MeJA treatment, JA
pathway genes, such as lipoxygenase (PgLOX), hydrogen
peroxidase lyase (PgHPL), allene oxide synthase (PgAOS),
and allene oxide cyclase (PgAOC1), and terpene pathway
genes, such as isopentenyl diphosphate isomerase (PgIPP)
and farnesyl diphosphate synthase (PgFPS), were highly
expressed and resulted in the accumulation of mono- and
sesquiterpenes. During mechanical wounding, PgLOX
expression was induced relatively late after 72 h of
& Deok-Chun Yang
[email protected]
Shadi Rahimi
[email protected]
1
Brain Korea 21 Plus Advanced Training Program,
Department of Biological Sciences, College of Natural
Sciences, Seoul National University, Seoul,
Republic of Korea
2
Graduate School of Biotechnology and Ginseng Bank,
College of Life Sciences, Kyung Hee University,
Yongin 446-701, Republic of Korea
3
Department of Oriental Medicinal Biotechnology, College of
Life Sciences, Kyung Hee University, Yongin 446-701,
Republic of Korea
treatment, however PgAOC1 was not induced. This resulted in decreased production of MeJA that in turn may have
lowered terpenoid production. In contrast, wounding ?
MeJA treatment increased PgAOC1 and PgLOX gene
expression earlier after 6 h and slowly promoted the production of mono- and sesquiterpenes. Furthermore, we
monitored the effect of MeJA upon wounding in in vitro
grown 1-month-old seedlings treated with MeJA ?
wounding. These result demonstrated that exogenous
MeJA is able to promote recovery from the wounding
effect by functioning as a long distance signal. Additionally, these results suggest that exogenous MeJA supplied at
the time of mechanical wounding prevents necrosis in the
ginseng leaves by increasing the production of terpenoids.
Keywords Allene oxide cyclase Lipoxygenase Long
signaling molecules Monoterpenes Sesquiterpenes
Abbreviations
JA
Jasmonic acid
MeJA
Methyl jasmonate
OGA
Oligogalacturonides
WR
Wound responsive
IPPI
Isopentenyl diphosphate isomerase
DMAPP
Dimethylallyl diphosphate
GPP
Geranyl diphosphate
FPS
Farnesyl diphosphate synthase
LOX
Lipoxygenase
HPL
Hydrogen peroxidase lyase
AOC
Allene oxide cyclase
AOS
Allene oxide synthase
GC–MS
Gas chromatography mass spectrometry
RT-PCR
Reverse transcription PCR
qRT-PCR
Quantitative real time-reverse transcriptase
polymerase chain reaction
123
DOI 10.1007/s11240-015-0838-8
ORIGINAL ARTICLE
Exogenous methyl jasmonate prevents necrosis caused
by mechanical wounding and increases terpenoid biosynthesis
in Panax ginseng
Sri Renuka Devi Balusamy1,2 • Shadi Rahimi2 • Johan Sukweenadhi2
Yu-Jin Kim2 • Deok-Chun Yang2,3
•
Received: 25 September 2014 / Accepted: 31 July 2015
Ó Springer Science+Business Media Dordrecht 2015
Abstract Upon mechanical wounding, plants locally
induce necrosis, accumulate methyl jasmonate (MeJA) and
acquire systemic resistance in nearby tissues. One-monthold in vitro grown Panax ginseng seedlings were treated
with either 50 lM MeJA or mechanical wounding alone or
a combination of both, to evaluate jasmonic acid (JA)
signaling and terpene biosynthetic pathway genes along
with terpenoid accumulation. After MeJA treatment, JA
pathway genes, such as lipoxygenase (PgLOX), hydrogen
peroxidase lyase (PgHPL), allene oxide synthase (PgAOS),
and allene oxide cyclase (PgAOC1), and terpene pathway
genes, such as isopentenyl diphosphate isomerase (PgIPP)
and farnesyl diphosphate synthase (PgFPS), were highly
expressed and resulted in the accumulation of mono- and
sesquiterpenes. During mechanical wounding, PgLOX
expression was induced relatively late after 72 h of
& Deok-Chun Yang
[email protected]
Shadi Rahimi
[email protected]
1
Brain Korea 21 Plus Advanced Training Program,
Department of Biological Sciences, College of Natural
Sciences, Seoul National University, Seoul,
Republic of Korea
2
Graduate School of Biotechnology and Ginseng Bank,
College of Life Sciences, Kyung Hee University,
Yongin 446-701, Republic of Korea
3
Department of Oriental Medicinal Biotechnology, College of
Life Sciences, Kyung Hee University, Yongin 446-701,
Republic of Korea
treatment, however PgAOC1 was not induced. This resulted in decreased production of MeJA that in turn may have
lowered terpenoid production. In contrast, wounding ?
MeJA treatment increased PgAOC1 and PgLOX gene
expression earlier after 6 h and slowly promoted the production of mono- and sesquiterpenes. Furthermore, we
monitored the effect of MeJA upon wounding in in vitro
grown 1-month-old seedlings treated with MeJA ?
wounding. These result demonstrated that exogenous
MeJA is able to promote recovery from the wounding
effect by functioning as a long distance signal. Additionally, these results suggest that exogenous MeJA supplied at
the time of mechanical wounding prevents necrosis in the
ginseng leaves by increasing the production of terpenoids.
Keywords Allene oxide cyclase Lipoxygenase Long
signaling molecules Monoterpenes Sesquiterpenes
Abbreviations
JA
Jasmonic acid
MeJA
Methyl jasmonate
OGA
Oligogalacturonides
WR
Wound responsive
IPPI
Isopentenyl diphosphate isomerase
DMAPP
Dimethylallyl diphosphate
GPP
Geranyl diphosphate
FPS
Farnesyl diphosphate synthase
LOX
Lipoxygenase
HPL
Hydrogen peroxidase lyase
AOC
Allene oxide cyclase
AOS
Allene oxide synthase
GC–MS
Gas chromatography mass spectrometry
RT-PCR
Reverse transcription PCR
qRT-PCR
Quantitative real time-reverse transcriptase
polymerase chain reaction
123