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PENGARUH PENAMBAHAN SERUM SAPI PADA MEDIA KULTUR TERHADAP KOMPETENSI MEIOSIS OOSITANJING DARI BERBAGAI STADIUM ESTRUS SECARA IN VITRO | Heru Fibrianto | Jurnal Sain Veteriner 286 143 1 PB

PENGARUH PENAMBAHAN SERUM SAPI PADA MEDIA KULTUR TERHADAP KOMPETENSI
MEIOSIS OOSITANJING DARI BERBAGAI STADIUM ESTRUS SECARA IN VITRO

INFLUENCE OF COWS SERUM ADDITION ON MEIOTIC COMPETENCE OF DOG OOCYT
FROM VARIOUS ESTRUS STADIAE IN I/ITRO

Yuda Heru tr'ibrianto', Claude Mona Airin', Asyhari', Amrullah Anindito', Nuraini Rachmawati',
Koko Kurniawan'o Pradityo Yoga Wibowo'
'Bagian

Fisiologi, Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
Email: fibrianto1802@gmail.com

ABSTRACT

Reproductiontechnologyachievementon caninewas fast developed,with born of cloning and transgenic
dog, unforfunately that'stechnology still usedoocytematuredin vivo becausecanine oocyte matwation in vitro
in this animalnot developedyet andmafurationratestill low. The presentstudywas to investigatedthe effectsof
cow serumsupplementationon meiotic resumptionof canineoocytesfrom various estms stage.Ovary obtained
at clinic veterinarypractice.Ovary were
from bitchesatan-,pro-and di-estrusstageafterovariohysterectomized

placed in 0.9o/ophysiologycal saline added 1% pennicilline-streptomycinwith temperature37'C and then
broughtto laboratory.Ovary washedseveraltimesusedPBS andoocyteswere obtainedby slicing ovariantissue
25mMHepes,1%pennicillin-streptomycin.Selected
intissueculturemedium(TCM)-l99supplementedwith
cummulusoocytecomplexes(COCs) were maturedin TCM-199 as control and TCM-1 99 + 10% cow serumas
treatment,on incubator with 38C, 5VoCO, and 5yo Or. After 72 h incubation and Hoechst 33342 staining, the
result shows that cow serum supplement have higher meiotic resumption significantly (P

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