Growth response of buffaloes treated with oestradiol 17 B
GROW
TH RESPONSE OF BUFFALOES TREATED WI TH.
OESTRADI OL 17 f 3
PRAPTI MAHYUDDI N
Resear ch I nst i t ut e f or Ani mal Pr oduct i on
P. O. Box 221, Bogor 16002, I ndonesi a
( Recei ved by t he edi t or 21 Jul y 1995)
ABSTRAK
MAHYUDDI N, P. 1995 . Respon per t umbuhan ker bau yang di per l akukan dengan est r adi ol 17 B. Jur nal I l mu Ter nak dan Vet er ner
1 ( 2) : 89- 93 .
Pengar uh est r adi ol 17 B di cobakan pada 10 ekoc ker bau dengan r at aan bobot badan 242 kg . Ter nak- t er nak t er sebut di ber i r umput gaj ah ad
l i bi t um dan pakan konsent r at sebanyak I % bobot badan, dan secar a acak di bagi dal am 2 keompok, masi ng- masi ng t er di r i dar i 5 ekoc, yai t u
Keompok
per l akuan dan Keompok kont r ol . Kel ompok per l akuan di i mpl ant asi t el i nganya dengan kar et si l i kon yang mengandung est r adi ol
ber akt i vi t as l ama, sel ama 200 har i . Ker bau yang di per l akukan dengan est r adi ol menunj ukkan kenai kan bobot badan ( 0, 89 vs 0, 68 ) ;g/ h) dai
ni sbah konver si pakan ( 9, 2 vs 11, 2) yang nyat a . Penggunaan pemacu per t umbuhan ( gr owt h pr omot ant ) i ni secar a ekonomi s mengunt ungkan .
Kat a kunci : Est r adi ol , ni sbah konver si pakan, ker bau
ABSTRACT
MAHYUDDI N,
P. 1995 . Gr owt h r esponse of buf f al oes t r eat ed wi t h oest r adi ol
17 B. Jur nal I l mu Ter nak dan Vet er i ner 1 ( 2) : 89- 93 .
The ef f ect of oest r adi ol 17 B was t est ed i n 10 buf f al oes, wei ghi ng appr oxi mat el y 242 kg . They wer e f ed el ephant gr ass ad l i bi t um pl us
concent r at e f eed at a r at e of 1 %body wei ght , and wer e r andoml y di vi ded i nt o 2 gr oups of 5, t he t r eat ed and cont r ol gr oups . The t r eat ed gr oup
ani mal s wer e i mpl ant ed wi t h a l ong act i ng oest r adi ol - si l i cone r ubber on t he l ef t ear f or 200 days . Oest r adi ol t r eat ed- buf f al oes showed a
si gni f i cant i ncr ease i n l i vewei ght gai n ( 0. 89 vs 0 . 68 kg/ d) and f eed conver si on r at i o ( 9 . 2 vs 11 . 2) . Cer t ai nl y i t has a si gni f i cant economi c
advant age i n usi ng t hi s gr owt h pr omot ant .
Key wor ds : Oest r adi ol , f eed conver si on r at i o, buf f al o
I NTRODUCTI ON
Resear ch f or t he l ast 20 year s r eveal ed t hat suppl ement at i on of r umi nant s on l ow qual i t y f or age has an
ef f ect pr oduct i vi t y t hr ough i ncr ease ef f i ci ency of f eed
ut i l i sat i on . The ef f i ci ency coul d be f ur t her i ncr eased i f
t he hor mone mi l l i eu i s f avour abl e t hat i s by r epar t i t i oni ng t he nut r i ent absor bed t o t he t ar get t i ssue.
Gr owt h pr omot ant s whi ch have been commer ci al l y
pr oduced by r ecombi nant DNA t echnol ogy has cl ear l y
i l l ust r at ed t he possi bl e mani pul at i on' of hor monal
mi l l i eu t o i ncr ease t he r at e and ef f i ci ency of gr owt h .
One of t hose i s oest r adi ol 17 B whi ch was f ound t o
af f ect t he ci r cul at i ng gr owt h hor mone
ENRI GHT
et al . ,
concent r at i on
and
hor mone r ecept or
st i mul at es
1990) and i nsul i n
t he
( BREI ER
muscl e
exper i ment by
number
pr ot ei n
DAW
SON
of
( TRENKLE
( TRENKLE,
hepat i c
1983,
1983)
gr owt h
et al . , 1988) . Thi s i n t ur n
deposi t i on as
shown i n
et al . , ( 1991) . However , t he
ef f ect of oest r adi ol i s onl y achi eved i f di et ar y pr ot ei n
l evel s ar e adequat e ( GI LL et al . , 1987, DAWSON et al . ,
1991) .
t r eat ed st eer s wi t h
oest r adi ol 17 B i mpr oved wei ght gai ns by 0 . 04 t o 0 . 1
kg/ d ( 7 t o 20 %) and WELLI NGTON ( 1985) r epor t ed
CHULDLEI GH
et
al .
( 1982)
si mi l ar i ncr eases f ol l owi ng t r eat ment wi t h zer anol or
oest r adi ol 17 B. SAWYER et al . ( 1987) compar ed t he
gr owt h r esponse of unt r eat ed st eer s cal ves wi t h t hose
gi ven a l ong act i ng oest r adi ol 17 B i n si l i cone r ubber
i mpl ant or i mpl ant s of zer anol or oest r adi ol - pr ogest er one f ound t hat oest r adi ol 17 B was t he onl y pr oduct
t hat si gni f i cant l y i ncr eased wei ght gai n compar ed t o
cont r ol ( 0 . 68 vs 0 . 52 kg/ d, 18 . 2% advant age) . Fur t her mor e, oest r adi ol 17 B showed a si mi l ar i ncr eases i n
wei ght gai n bot h at l ow and hi gh pl anes of nut r i t i on
suggest i ng t hat t he i mpl ant mai nt ai ni ng a cont i nuousl y
hi gh l evel of ci r cul at i ng anabol i c agent dur i ng t he
exper i ment . Al t hough t her e have been r epor t s of t he
ef f ect of gr owt h pr omot ant s on cat t l e gr owt h, r epor t s
on buf f al o have not been avai l abl e .
The f ol l owi ng exper i ment was desi gned t o t est t he
ef f ect of oest r adi ol 17 B on t he l i vewei ght gai n and f eed
conver si on r at i o i n buf f al o .
MATERI ALS AND METHODS
Ani mal s and exper i ment al desi gn
Ten buf f al oes ( 4 st eer s and 6 hei f er s) wer e assi gned
i nt o t wo t r eat ment gr oups i n st r at i f i ed r andomi sat i on
89
PRAPTI MAHYUDDI N : Gr owt h Res pons e of Buf f al oes Tr eat ed wi t h Oest r adi o117, 6
accor di ng t o body wei ght . The t wo t r eat ment gr oups
wer e : I , Cont r ol and I I , Oest r adi ol ( Compudose)
measur ed accor di ng t o
GOERI NG and VAN SOEST
( 1970)
met hod.
i mpl ant . The i mpl ant consi st s of a 3 c m l ong si l i cone
r ubber cover ed wi t h a speci al l ayer of si l i cone r ubber
i nt o whi ch i s i mpr egnat ed a 24 mg cr yst al s of oest r adi ol
17 8 . I t i ncor por at es a uni que del i ver y syst em t o ensur e
an even r el ease of t he nat ur al hor mone over a 200 day
per i od . The i mpl ant was i nser t ed subcut aneousl y i n t he
mi ddl e of t he back of buf f al o' s l ef t ear .
St at i st i cal anal ysi s
Di f f er ences
( 50%) , coconut meal ( 8%) , cor n meal ( 11 . 596) , pal m
ker nel cake ( 6%) , cocoa shel l ( 740) , soy bean meal
( 4%) , kapok seed meal
( 5 . 5%) , f i sh meal ( 3%) and
mi ner al vi t ami n mi x ( 5%) . Dat a on t he chemi cal composi t i on of el ephant gr ass and concent r at e f eed i s
pr esent ed i n Tabl e 1 . Wat er was avai l abl e at al l t i mes .
t he
i mpl ant at i on
of
RESULTS AND DI SCUSSI ON
gr ass ( Penni set um pur pu-
r eum) ad l i bi t um pl us concent r at e f eed at a r at e of 196
body wei ght . The concent r at e f eed was made by t he
l ocal dai r y cooper at i ve consi st i ng of wheat pol l ar d
to
St udent ' s t t est . Al l dat a wer e comput ed usi ng t he
St at i st i x 3 . 0 pr ogr am ( NH Anal yt i cal Sof t war e, USA) .
The ani mal s wer e kept i n i ndi vi dual cages and wer e
of f er ed chopped el ephant
at t r i but ed
oest r adi ol 17 13 wer e eval uat ed agai nst t he cont r ol by
Ther e wer e t endency
t hat
l i vewei ght
gai n,
dr y
mat t er i nt ake per uni t l i ve wei ght ( DMI / LW) and f eed
conver si on r at i o i n oest r adi ol - i mpl ant ed ani mal s wer e
i mpr oved compar ed t o t hat i n non- i mpl ant ed ani mal s .
However ,
t hose par amet er s wer e onl y si gni f i cant at
P
TH RESPONSE OF BUFFALOES TREATED WI TH.
OESTRADI OL 17 f 3
PRAPTI MAHYUDDI N
Resear ch I nst i t ut e f or Ani mal Pr oduct i on
P. O. Box 221, Bogor 16002, I ndonesi a
( Recei ved by t he edi t or 21 Jul y 1995)
ABSTRAK
MAHYUDDI N, P. 1995 . Respon per t umbuhan ker bau yang di per l akukan dengan est r adi ol 17 B. Jur nal I l mu Ter nak dan Vet er ner
1 ( 2) : 89- 93 .
Pengar uh est r adi ol 17 B di cobakan pada 10 ekoc ker bau dengan r at aan bobot badan 242 kg . Ter nak- t er nak t er sebut di ber i r umput gaj ah ad
l i bi t um dan pakan konsent r at sebanyak I % bobot badan, dan secar a acak di bagi dal am 2 keompok, masi ng- masi ng t er di r i dar i 5 ekoc, yai t u
Keompok
per l akuan dan Keompok kont r ol . Kel ompok per l akuan di i mpl ant asi t el i nganya dengan kar et si l i kon yang mengandung est r adi ol
ber akt i vi t as l ama, sel ama 200 har i . Ker bau yang di per l akukan dengan est r adi ol menunj ukkan kenai kan bobot badan ( 0, 89 vs 0, 68 ) ;g/ h) dai
ni sbah konver si pakan ( 9, 2 vs 11, 2) yang nyat a . Penggunaan pemacu per t umbuhan ( gr owt h pr omot ant ) i ni secar a ekonomi s mengunt ungkan .
Kat a kunci : Est r adi ol , ni sbah konver si pakan, ker bau
ABSTRACT
MAHYUDDI N,
P. 1995 . Gr owt h r esponse of buf f al oes t r eat ed wi t h oest r adi ol
17 B. Jur nal I l mu Ter nak dan Vet er i ner 1 ( 2) : 89- 93 .
The ef f ect of oest r adi ol 17 B was t est ed i n 10 buf f al oes, wei ghi ng appr oxi mat el y 242 kg . They wer e f ed el ephant gr ass ad l i bi t um pl us
concent r at e f eed at a r at e of 1 %body wei ght , and wer e r andoml y di vi ded i nt o 2 gr oups of 5, t he t r eat ed and cont r ol gr oups . The t r eat ed gr oup
ani mal s wer e i mpl ant ed wi t h a l ong act i ng oest r adi ol - si l i cone r ubber on t he l ef t ear f or 200 days . Oest r adi ol t r eat ed- buf f al oes showed a
si gni f i cant i ncr ease i n l i vewei ght gai n ( 0. 89 vs 0 . 68 kg/ d) and f eed conver si on r at i o ( 9 . 2 vs 11 . 2) . Cer t ai nl y i t has a si gni f i cant economi c
advant age i n usi ng t hi s gr owt h pr omot ant .
Key wor ds : Oest r adi ol , f eed conver si on r at i o, buf f al o
I NTRODUCTI ON
Resear ch f or t he l ast 20 year s r eveal ed t hat suppl ement at i on of r umi nant s on l ow qual i t y f or age has an
ef f ect pr oduct i vi t y t hr ough i ncr ease ef f i ci ency of f eed
ut i l i sat i on . The ef f i ci ency coul d be f ur t her i ncr eased i f
t he hor mone mi l l i eu i s f avour abl e t hat i s by r epar t i t i oni ng t he nut r i ent absor bed t o t he t ar get t i ssue.
Gr owt h pr omot ant s whi ch have been commer ci al l y
pr oduced by r ecombi nant DNA t echnol ogy has cl ear l y
i l l ust r at ed t he possi bl e mani pul at i on' of hor monal
mi l l i eu t o i ncr ease t he r at e and ef f i ci ency of gr owt h .
One of t hose i s oest r adi ol 17 B whi ch was f ound t o
af f ect t he ci r cul at i ng gr owt h hor mone
ENRI GHT
et al . ,
concent r at i on
and
hor mone r ecept or
st i mul at es
1990) and i nsul i n
t he
( BREI ER
muscl e
exper i ment by
number
pr ot ei n
DAW
SON
of
( TRENKLE
( TRENKLE,
hepat i c
1983,
1983)
gr owt h
et al . , 1988) . Thi s i n t ur n
deposi t i on as
shown i n
et al . , ( 1991) . However , t he
ef f ect of oest r adi ol i s onl y achi eved i f di et ar y pr ot ei n
l evel s ar e adequat e ( GI LL et al . , 1987, DAWSON et al . ,
1991) .
t r eat ed st eer s wi t h
oest r adi ol 17 B i mpr oved wei ght gai ns by 0 . 04 t o 0 . 1
kg/ d ( 7 t o 20 %) and WELLI NGTON ( 1985) r epor t ed
CHULDLEI GH
et
al .
( 1982)
si mi l ar i ncr eases f ol l owi ng t r eat ment wi t h zer anol or
oest r adi ol 17 B. SAWYER et al . ( 1987) compar ed t he
gr owt h r esponse of unt r eat ed st eer s cal ves wi t h t hose
gi ven a l ong act i ng oest r adi ol 17 B i n si l i cone r ubber
i mpl ant or i mpl ant s of zer anol or oest r adi ol - pr ogest er one f ound t hat oest r adi ol 17 B was t he onl y pr oduct
t hat si gni f i cant l y i ncr eased wei ght gai n compar ed t o
cont r ol ( 0 . 68 vs 0 . 52 kg/ d, 18 . 2% advant age) . Fur t her mor e, oest r adi ol 17 B showed a si mi l ar i ncr eases i n
wei ght gai n bot h at l ow and hi gh pl anes of nut r i t i on
suggest i ng t hat t he i mpl ant mai nt ai ni ng a cont i nuousl y
hi gh l evel of ci r cul at i ng anabol i c agent dur i ng t he
exper i ment . Al t hough t her e have been r epor t s of t he
ef f ect of gr owt h pr omot ant s on cat t l e gr owt h, r epor t s
on buf f al o have not been avai l abl e .
The f ol l owi ng exper i ment was desi gned t o t est t he
ef f ect of oest r adi ol 17 B on t he l i vewei ght gai n and f eed
conver si on r at i o i n buf f al o .
MATERI ALS AND METHODS
Ani mal s and exper i ment al desi gn
Ten buf f al oes ( 4 st eer s and 6 hei f er s) wer e assi gned
i nt o t wo t r eat ment gr oups i n st r at i f i ed r andomi sat i on
89
PRAPTI MAHYUDDI N : Gr owt h Res pons e of Buf f al oes Tr eat ed wi t h Oest r adi o117, 6
accor di ng t o body wei ght . The t wo t r eat ment gr oups
wer e : I , Cont r ol and I I , Oest r adi ol ( Compudose)
measur ed accor di ng t o
GOERI NG and VAN SOEST
( 1970)
met hod.
i mpl ant . The i mpl ant consi st s of a 3 c m l ong si l i cone
r ubber cover ed wi t h a speci al l ayer of si l i cone r ubber
i nt o whi ch i s i mpr egnat ed a 24 mg cr yst al s of oest r adi ol
17 8 . I t i ncor por at es a uni que del i ver y syst em t o ensur e
an even r el ease of t he nat ur al hor mone over a 200 day
per i od . The i mpl ant was i nser t ed subcut aneousl y i n t he
mi ddl e of t he back of buf f al o' s l ef t ear .
St at i st i cal anal ysi s
Di f f er ences
( 50%) , coconut meal ( 8%) , cor n meal ( 11 . 596) , pal m
ker nel cake ( 6%) , cocoa shel l ( 740) , soy bean meal
( 4%) , kapok seed meal
( 5 . 5%) , f i sh meal ( 3%) and
mi ner al vi t ami n mi x ( 5%) . Dat a on t he chemi cal composi t i on of el ephant gr ass and concent r at e f eed i s
pr esent ed i n Tabl e 1 . Wat er was avai l abl e at al l t i mes .
t he
i mpl ant at i on
of
RESULTS AND DI SCUSSI ON
gr ass ( Penni set um pur pu-
r eum) ad l i bi t um pl us concent r at e f eed at a r at e of 196
body wei ght . The concent r at e f eed was made by t he
l ocal dai r y cooper at i ve consi st i ng of wheat pol l ar d
to
St udent ' s t t est . Al l dat a wer e comput ed usi ng t he
St at i st i x 3 . 0 pr ogr am ( NH Anal yt i cal Sof t war e, USA) .
The ani mal s wer e kept i n i ndi vi dual cages and wer e
of f er ed chopped el ephant
at t r i but ed
oest r adi ol 17 13 wer e eval uat ed agai nst t he cont r ol by
Ther e wer e t endency
t hat
l i vewei ght
gai n,
dr y
mat t er i nt ake per uni t l i ve wei ght ( DMI / LW) and f eed
conver si on r at i o i n oest r adi ol - i mpl ant ed ani mal s wer e
i mpr oved compar ed t o t hat i n non- i mpl ant ed ani mal s .
However ,
t hose par amet er s wer e onl y si gni f i cant at
P