Seoul lnternational Conference on Life Sciences and Biological Engineering

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SICEAS
Seoul International Conference on
Engineering and Applied Sciences

SICLSBE
Seoul International Conference on
Life Sciences and Biological En!ineering

SICSSAM
Seoul International Conferenil on
Social Sciences and Management

APCESP
Asia-Pacific Conference on
Education, Society and psychology

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HIGHER EDUCATION FORUM

www.prohef.org
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Certihc ate of Fre sentatron

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Seoul lnternational Conference on Life Sciences and Biological Engineering

Janua|Y 5-7, zot6, Seoul
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Nurdiana Nurdiane
Medical Faculty, Brawijaya Universitv
Has attended the conference and presented a paper entitled

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PreChief Executive Committee

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Conference program

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fanuary 5-7,2016

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Seoul, Korea

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seoul International conference on social
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APCESP
Asia-Pacific conference on Education,
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seoul International conference on Life
sciences and

Biological Engineering
SICEAS

seoul International conference on Engineering
and
Applied Sciences

SICSSAM

seoul International conference on social sciences and Management
ISBN 978-986-5654-10-8

APCESP

Asia-Paciftc Conference on Education, Societ5r and Prycholory
ISBN 978-986-5654-08-5

SICLSBE

seoul International conference on Life sciences and BiologF€l Engineering

ISBN 978-986-5654-19-1

SICEAS

seoul International conference on Engineering and Applied sciences
ISBN 978-986-5654-09 -2

Content
General Information for participants ......,........
Organizing Committees............ r......,..

..........5

7

Interrrational Committee of SICLSBE
International Committee of SICEAS........
International Committee of SICSSAM

......................... 7

.....9

Special Thanks to Session Chairs......
Conference Venue Information..............

10

tt

Conference Schedule

.............12

Natural Sciences Keynote Speech
Soclal Sciences Keynote Speech

t4
18

OraI

..2A

Educadon (1)

Society(l) / Communication.....
Hfe Sclences (1) / Chemical Engineering (1)
Computer & Informadon Engineering and
Technologf
Biological Englneering / Chemical

.1....r..r......rr. r.r.

(1)

20

......2s

Mechanical Engineering and
Psycholory (1)
Management (1)/ Economics

/Finance
32
society (2)/ Potitics /
34
Prychology (2) / Culture.....................
................... 36
Applied Sciences (1) / Civit Engineering
Materials
I
Science and Engineering...3g
Management (2)
40
Applied Sciences (2) / Computer & Information
Engineering and Technolo
W e)
/ Electrical and Electronic Engineering
Education (2)
44
Life Sciences (2)
.............45
Management (3)
47
Psycholqy (3)/ Educarion (3)
49
Poster Session (1)
51
Applted Sciences/ Clvil Engineering
/ Electrical and Electronic Engineering/
Chemical Engtneering/ Materiats
Science and Engtneering/ Mechanical
Engineering and Technologr/ power
& EnerryEngineering.............. .....
51

3

I

Poster Session t2) ..............,r.r...r.r..r,.r, .......,...........
Educaffon I Soeletyl Computer Engineeringand Technology/ Life

Sciences/Management/

Psycholory.,,.......,r..,......r,r,,.
}

4

-.......'....'..54
.......rr.,..r.......,,

54

r

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I

eneral Information

for Participants

Registration

The registration desk

witl be situated on the 4F at the Courtyard by ll[arriott Seoul Times Square

during the following time:

08:30-16:30 Wednesday, January 6, 2016
08:30-16:30

Thurdan Jauuary 7, 2016

I Organizer
IrvE:.-

ir

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Higher Education Forum (HEF)

Tel: + 886 2 2740 1498 [ www.prohef.ors

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A Polite Request to All participants
Participants are requested to arrive in a timely fashion for all addresses,
whether to their own, or to
those of other presenters. Presenters are reminded that the time slots
should be divided fairly
and

equally between the number of presentations, and that they should not ovemrn.
The session chair is
asked to assume this timekeeping role and to summarize key issues
in each topic.

Sandals or 5lippers

TankTop

5

Shorts

I

Preparetion for Oral Presentetions
All presentation rooms are equipped with a scrsen, an LCD projector, and a laptop computer installed
with Microsoft PowerPoint. You will be able to insert your USB flash drive into the computer and
double check your file in PowerPoint. We recommend you to bring two copies of the file in case that
one fails. You may also connect your own laptop to the provided projectoq, hqwevgcplease ensure you
have the requisite connector.

I

Preparation for Poster Presentation
Materiats Provided by the Conference Organizer:

1.

X-frame display & base fabric canvases (60cmxl60cm)

2.

Adhesive tapes or binder clips

Materials Prepared by the Presenters:

l.

Home-made Poster(s)

2. Material: not limited, can be posted on the canvases
3. Recommended poster size: 60cm*160cm
t

A 60srn*160cm poster illustrates

l. Wider than 60cm (left)

the research findings.

2. Copy of PowerPoint slides inA4 papers (right)

6

t

Organizing Committees
International Committee of SICLSBE
Ahmad ZuhairiAMullah, School of Chemical Engineering, Universiti Sains Malaysia

FadzilahAdibahAbdul Maji4 Departrnent of Bioprocess Engineering, Universiti Teknologi
Malaysia
Mohd Farid binAtan, Deparffnent of Chemical Engineering and Energy Sustainability,
University of Sarawak Malaysia
Sue-Joan Chang, Department of

Life Sciences, National Cheng Kung University

Yen-Chgng Chang, Institute of Molecular Medicine, National Tsing Hua University
Yun-Peng Chao, Deparhent of Chemical Engineering, Feng ChiaUniversity
Pei-Jen Chen, Departnent of Agriculhral ChemistryNational Taiwan University

H.M. El-Shora, Departrnent of Botany, Mansoura University

I-Ming Hsing, Departnent of

C-tremical and Biomolecular Engineering, The Hong Kong

University of Science and Technology
Shang-Da Huang, Deparfnent of Chemical, National Tsing Hua University

Bing Joe Hwang, Department of Chemical Engineering,ational Taiwan University of Science
and Technolory

Deparhent of Chemical Engineering, Sungkyunloran University
Li-Fen Lei, Departrrent ofAgricultural Economics, National Thiwan University
yunsheng Lou, College ofApplied Meteorology, Nanjing University of Information Science
Sungiee Kim,

and Technology

Mihir Kumar ptgkait, Departnent of Chemical Engineering, Indian Institute of Technology
Guwahati
Chgng-Suog Thn, Deparfinent of Chemical Engineering, National Tsing HuaUniversity

Tewin Tencomnao, Deparfinent of Ctinical Chemistry, Chutalongkorn University
S.K. Tripathi, Department of Water Resotrce,tndian Institute of Technology Poorkee
Henry N.C. Wong, Department of Chemical, The Chinese University of Hong Kong
Yusri b. Yusup, School of Industrial Technology, Universiti Sains Malaysia

HasanAkhtar Zudr,University School of Chemical Technology, Gtru Gobind Singh
Indraprastha UniversitY

7

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International Committee of SICEAS
Samit Bhattacharya, Indian Institute of Technology Kanpur

A. P. Shashikal4 Birla Institute of Tirchnology
;

RM. Senthamarai, Annamalai University
Arnab Bhaftachary4Indian Institute of Technology Kanpur
Zbigniew Michalewicz, University of Adelaide
B. Bhattacharya, Indian Institute of Technology Kharagpur

Amin Heidarpour, Monash University
Faizal Mustapha, Universiti Puta Malaysia

I

fnternational Committee of SICSSAM
Pedro Gardete, School of Business, Stanford University
Pascal Pailld,

Deparhent of Managemen! universitd Laval

Deniss Tsi ChungYer:ng, Wintec University

Hui Du, Beijing Jiaotong University
wan Khairuzzamar v/an Ismail, universiti Teknologi Malaysia
Xiang Yu, University of Science and Technology
Ding Qing, Singapore University of Management
Yuntao Chen" Berjing Jiaotong University

David Ding, Massey University
Lee Kong Chian, Siagapore University of Management
Jianwen 7.hang, Beijing University of Chemical Technology
Sanjay K. Jain, University of Delhi
Sanhong Deng, Nanjing University

Gengmiao Zhang,Xi' an Jiaotong University

9

Special Thanks to.Sessf on Chairs
Junaid Sarfraz Khan

University of Health Sciences Lahore Pakistan

Salithamby Abdul Rauff

Dhofar University

TitinAndri Wihastuti

Brawijaya University

Akio Tsuneda

Kumamoto University

Fatchiyah Fatchiyah

Brawijaya University

Geunho Lee

University of Miyazaki

Mariko Kikutani

Doshisha University

Hueiting Tsai

National Cheng Kung University

Aie-Rie Lee

Texas Tech University

Glenn Medallon Calaguas

Pampanga State Agricultural Univsrsity

Asmida Ismail

Universiti Teknologi MARA

Xubing Zhatg

Hong Kong Polytechnic University

Wichian Boonyaprapa

Mahidol University

Minnie Maliwat Liangco

Pampanga State Agricultural University

Chantana Khensri

Kasetsart University

Ikhlas A. Abdalla

Kuwait University

Riselligia Caninsti

YARSI University

l0

Life Sciences (U

/ Chemical Engineering (1)

Wednesday, January

6,2016

10:45-12:15

Room 2

Session Chair: Prof. Titin Andrt Wihastutt
SICLSBE-127
The Effect of Darapladibon LpPLA2 and Adhe ion Molecule Expres*ion in
Dyslipidemic-Model Rattus Novergicus Sprague Dawley Strain
Titin Andri Wihastuti I Browijaya University
Teuku Heriansyah I Brawijrya University

Aulani'Am I Brawijaya University
Nurul Cholifb.h Luthfiana I Brawijaya Untversity
SICLSBE-129

In YitroAna$sesAlpha- and Gamma-Guaiene Isolate Pogortemon llerbs for
Cyclooxygenase fsoenzyme Inhibitor on Pre-Osteoblast Cells
Sentst Joko Raharjo I Brawtjaya University

Nwdianalrlurdiana I Brawtjqta University
FatchiyahFatchiyah I Bra'$, tj rya Univer sity

SICLSBE-I31
The Local Goat Immature Oocytes Resources and Potential Preanthral Follicle Culture
System as an,{,Iternative Source of Ooeyteo Motured in

Vitro

Gatot Ciptadi I Brawijaya University
Mudawamah G.Ciptadi I llslamtc University of Malang
Muhamad Ntn Ihsan I Brawijrya Untversity

Yayuk Sri Rahayu I Brawijrya University

Budi Siswanto I Brawijrya University

SICLSBE.l35
DNA Barcoding of the Good Pcrformance-seleeted Hybrids Derived from Interspecific
Hybridization of Plants in the Genus Jatrophr
Nutchanun Salcthanutp at I Kns e t s art Univer s tty
Vipa Hongtrakul I Kasetsart Unfuersity

z5

SICEAS.35E

Influence of Extracting $olvento or Its Antioxidant Properties of Bawang Dayak
@leutherine Palmifolia L' Merr)
Angg RegianaAgustin I Nattonal Tatwan (Iniversity of Science and Technologt
Sitti Faika I National Tatwan {Jniversity of Science and Technologt i i *
Yi-Hsu Ju I Nattonal Taiwan {lntversity af science and Technologt
SICLSBE.lSO
Standardization of Macropropagation Technique of Anogeissus Pendula A Recalcitrant
Tree Species through Stem Branch Cuttirgs
S.K.Tiwari I Forest Genetics Plant Propagatton & Biotechnologt Division
State Forest Research Institute

G. KrishnamurthyAmit Pandey I Forest Genetics Plant Propagation & Biotechnologt

Divtston
State Forest Research Institute

Maneesh Pnri Goswanri I Forest Genetics Plant Propagation & Biatechnologt Division
State Forest Research Institute

::

..:

24

Seoul Internalional Co4ference on Engineering arul Applieil Science
South Korca" 5-7 lanuary 2016

In Vitro analyses alpha- and gamma-guaiene isolate Pogostemon Herbs for
Cyclooxygenase Isoenzyme Inhibitor on pre-osteoblast cells
Sentot Joko Raharjo(rx2),Nurdirna Nurdiana(3) and Fatchiyah Fatchiyahtax')

rBiology Doctoral Programe, Faculty ofscience. Brawijaya
University. Jl. Veteran I Malang 65l4l,lndonesia;
'Academic ofPharmacy and Food Analysis, Putra Indonesia Malalg, Jl. Barito 5 Malang 65123, lndonesia;
rMedicine Pharmacology,
Faculty of Medicine, Brawijaya univenity, Jl. veteran Malang 6514llndonesia;
aBiology Departm€nt, Faculty
of Science, Brawijaya tniversity, , Jl. Veteran Malang 65-14l Indonesia.
*Correspondence should be
addressed to Fatchiyah Fatchiyah, Phone/Fax: +623415757838, mail: fatchiya@ub.ac.id

ABSTRACT
T9 glnlore the selectivity of alpha-guaiene (CID519743) and gamma-guaiene (CID94275) as
inhihit cyclooxygenase (coX-l/ cox-2) on pre-osteoblast cells. IsolatJ of alpha- and gammaguaiene were obtaining fractional-vacuum distillation patchouli oil by piiodist-lo4: Eight
diflerent groups were used in this research: control, LPS induction, three different concentration
of fraction (20, 60, 180 pM) and LPS together with three different concentration offraction. The
expression of coX-l and coX-2 were using immunohistochemical single staining and
measured color value by confocal Laser Scanning Microscope (CLSM). Aipha-guaiene
and
gamma-guaiene fractions obtained from patchouli oil in fraction-3
ani
fraction-g
@8:.56r/")
(82.12%), respectively. Similar result was obtained from the effect of alpha-guiene fraction
to
the expression of COX-1 and COX-2. The increasing concentration showed a de"reasing effect
on both expressions. Different result was resulted from gamma-guaiene fraction to the
expression of coX- I and coX-2- Gamma-guaiene was relatively elevating the expression
of
COX-I when the concentration increased. Vice versa, it was decreasing the Jxpression ofCOX2. The fractions of alpha- and gamma-guaiene were affected the

ortox-t

and

coX-

"rpr..iion
2 in dose-dependent manner and different activities. Alpha guaiene
was showed inhibition to
coX- 1 and cox-2. In contrast, gamma-guaiene wa".esolted selective inhibition to coX-2.

Keyword:

selectivity, alpha-guaiene (cID519743), gamma-guaiene (ctDg4275),
COX-2 expression, inhibitor selective

cox-l/

I.

INTRODUCTION

Patomechanism osteoporosis is a disorder
of bone tumover involving cell function to
achieve coordination and balance resorption
of old bone (osteoclasts) and the formation
of new bone (osteoblasts) to the local
conditions, coordinated and sequentially as
the coupling process. Bone reorganization
initiated by the recruifinent and actrvation of
osteoclasts. Osteoblast cells as bone-forming
also play a role of producing prostaglardinE2 (PGE2), mainly related to inflammation
and act as a stimulator of bone resorption
[ 1]. Furthermore, Osteoclastogenesis occurs
osteoblasts to secrete Receptor Activated

Nuclear Kappa Ligand (RANKL), which
responded by osteoclasts with the formation
Receptor Activated Nuclear Kappa
(RANK), so the due to the interaction of
RANKL and RANK, then into mature
osteoclasts, so it can function in the process
of bone resorption. When bone resorption is
considered suffi cient then osteoblasts secrete
Osteoprotegerin (OPG)
binding to
RANKL, untied of RANK-RANKL and
osteoclasts undergo apoptosis. pre-

of

for

cells have differentiated
characteristics polygonal shape, fusiform
cells become maflre osteoblasts. The mature
osteoblast cells will undergo mineralization
with hydroxyapatite deposition, produce
osteoblast

osteoid matrix. Pre-osteoblast

cells
expressing alkaline phosphatase which plays
a role in bone mineralization [2].
On pre-osteoblast cell cultue, NSAID
compounds were capable of inhibiting the
synthesis of PGE2 and once served suppress

osteoclastic bone resorption RANKL-

dependent associated with inflammation [3].
In such a mechanism, mPGES-1 also plays a
role in the synthesis of PGE2 in cellmediated differentiation of osteoblasts and
osteoclasts PGE [4]. Analysis of in-vitro, in
lipopolysaccharide-induced osteoblast cells
showed that mPGES-1 plays a role in the
sl,nthesis of PGE2 in bone degradation due

to inflammation [,5]. Prostaglandins (PG)
are known to play a role in the pathogenesis

of inflammation and inflammatory pain. PG
plays a central role in the peripheral and

inflammalory processes,

nociceptor
generation
sensitization, and the
of pain. PG

is a target in the inhibition of

cyclooxygenase (COX), which inhibit the
inflammatory process and analgesic. Two
isoforms of COX have been identified;
COX-I is constitutively distributed in most
tissues and
responsible
the

is

for

physiological production of PG, whereas
COX-2 is induced in a variety of cell t1pes,
including chondrocytes cells when exposed
to cytokines, mitogens, and endotoxins.
Conventional NSAID drugs have the ability
as an inhibitor of COX-I and COX-2 at a
dose of antiinflammatory and this dual
inhibition of gastrointestinal side effects.
Effects of NSAIDs were an ability of the

drug

in

inhibiting the

cyclooxygenase

(COX) and the production of prostaglandins
that are important mediators of pain and
inflammatory response. COX eruyme
facilitates the metabolism of arachidonic
acid become to prostaglandins H2, and then
metabolized by prostaglandin-E synthase
produce prostaglandin-B2 (PGE2). NSAID
compounds, such as Celecoxib (SC-58 635)

in

as selective COX-2 inhibitors
the
treatment of OA. The drugs was the only
drug that has been approved by the FDA
[6,7]. However, the lack of selective COX-2
inhibitors cause side effects increase blood
pressure and increase the risk of heart attack
of drugs selective inhibitors of COX-2 [8,9].
The side effects caused great concern, so the
need for methods and compounds of natural
ingredients that can be developed as the
design of drugs inhibiting COX-I and COX2 selective.
Alpha-guaiene (CID5|97 43) and gammaguaiene (CID9 4275) werc
class of
sesquiterpenoid compound groups as one of
the main components and structure isomers

a

(Molecular Weight

:

2A4 El mol) of

patchouli oil compounds from Pogostemon
cablin Benth. The other major patchouli oil
component was alpha-patchouli alcohol.
Alfa-patchouli alcohol had anti-

in{lammatory activity

in

macrophages

RAW264.7 cells [10]. Alfa-bulnesene had
the ability as an anti-aggregation ptalete on
rabbit blood by inhibiting the COX enzyme
mechanisms and PAF (Platelet Activating
Factor) [11,12]. The bioactivity of some
compounds from natural materials unclearly,
such as the bioactivities of alpha- and
gamma-guaiene as a COX-I/ COX-2
inhibitor selective on pre-osteoblast cells.
The purpose of this research is to explore the
potential of alpha-guaiene (CID519743) and
gamma-guaiene (CD94275) as inhibits
COX-I and COX-2 isoenzyme on preosteoblast cells (MC3T3E1) induced by
LPS.

2. MATERIAL AND ilIETHODS
2.1 Fraction of alpha-guaiene

and

gamma-guaiene

Patchouli oil produced from

steam

distillation of patchouli leaves (Pogostemon
cablin Benth) in home industry TrenggalekEast Java, Indonesia. Isolation of alphaguaiene and gamma-guaiene were using
fractional-vacuum distillation by PiloDistIO4-Germany in Chemical Research Center,
LIPI, Serpong, East Java, Indonesia. The
fractionation results were analyzed by Gas

Chromatography

Mass

Spectrometry
Shimadzu QP-2010 with RTX-Wax column,
detector MS and WileyS Library in the

Organic Chemistry Laboratory, Brawijaya
University.

2.2 The affect alpha- and gamma-guaiene
fractions to expression of COX-I dan
COX-2 on pre-osteoblast cells.

Pre-osteoblasts cell used is preosteoblasts cell MC3T3E1 sub clone 4 from

the American Type Culture Cell. Preosteoblasts cells were grown in complete
media alfa-MEM, 2mM L-glutamine, I mM
sodium pynrvate, rc% FBS and lAo/o
penstrep in the bottle flask 25 cm2 and
incubated in incubator COr 5olo, temperature
of 37o C until 70-80% confluent cells. Once
confluent, the cells are ready to be treated
[13]. Culture of pre-osteoblast cells cultured
in well plates to achieve optimal growth.
Then the cell cultures were divided into
normal eight group, LPS group (10 mg/
mL), the treatrnent group fraction of alphaguaiene isolate (fraction-5) and gamma-

guaiene isolate (fraction-8)(concentration:
20,40, and 60 pM) and LPS treatment group
(10 mg/ mL) + fraction of alpha-guaiene
isolate (fraction-5) and gamma-guaiene
isolate (fraction-8) (concentration 20, 40,
and 60 pM) to the protein COX-I/ COX-2
expression. Immunohistochemical staining
performed single staining against COX-1
and COX-2 expression. The results of
immunohistochemical staining were
visualization and measured color value
using Confocal Laser Scanning Microscope
(CLSM). Furthermore observed 3-field of

view and then calculated the

average

expression of proteins in three replications
sample pre-osteoblast cells.
3. RESULT

3.1 Isolation of

alpha-guaiene and

gamma-guaiene.

Alpha-guaiene isolate and gammaguaiene isolate from patchouli oil were
using vacuum fractional distillation by

PiloDistl 04-Germany. Identification isolates
of alpha-guaiene (CID519743) and gammaguaiene (CD94275), patchouli oil, and

other fraction were using GC-MS, as
presented in Figure 1. The fragmentation
analysis of alpha-guaiene (CID519743) and
gamma-guaiene {CID94275) compounds
were using GC-MS, as showed in Figure 2.
the rnass spectrometry
The results

of

analysis of the alpha-guaiene (fraction-3)
were using GC-MS with TIC retention time
: 21.60 minutes. EI mass specha analysis
results with m/ z:204 (base),189,175,161,

147, 133, 119, 105, 93, 79, 67,

55.

Simulation of the fragmentation pattern is
presented in Figure 2. The spectra data of
the EI-MS analysis results in accordance
with literature data gamma-guaiene t14l
The puri$ of alpha-guaiene (CID519743) in
fraction-3 was 48.56%, is presented in GCMS analysis (Figure 1). The results of the
mass spectrometry analysis of the gammaguaiene were using GC-MS with TIC
retention time : 22.64 minutes. EI mass
spectra analysis results with 204 (base),189,
175, 16l, 147, 133, ll9, 107,93,67, 55.
Simulation of the fragmentation pattern is
presented in Figure 2. The spectra data of
the EI-MS analysis results in accordance
with literature data gamma-guaiene tl4].
The fragmentation pattern of this compound
was simulated as follow: started with
termination of these compounds through the
release of CHr radicals in peak molecular
ion (Itf), so that the peak rnlz: (h,f-15) :
189. The termination of these compounds
through the release of CHr radicals in
molecular ion peak (lrrl+) so that the peak of
mlz: trrt'-tS: 189. The peak af mlz:lvf15

:

189, CHz release occurred successively

to obtain peak m/z : 133 was followed by
the release of CzF{+ in order to obtain the
peak of mlz: 107. The peak mlz : 147 to
experience the release of CzFIa to obtain

peakmlz:

119, followed by the release of
CzHz to obtain pakmlz:91, as showed on
Figure 2 (C-l and C-2). UV-Vis Spectra: nogamma-guaiene
absorption (structure
does not have auxochrome and chromophore
groups). IR Spectra (neat) u,*" (KBr) cm-l
3417, 1645, 1575, 1519, 1269, 1158 was

of

:

indicate alkenyl CH stretch, alkenyl C:C
stretch, alkyl and C-H stretch. Analysis of
NMR spectrum was exhibit two broad
singlet's at 6/ ppm 2.32-2.18 (2H, exocyclic

methylene), and two near-singlet's at 6/ ppm
0.90 and 0.84 (6H, two methYl groups

attached to double bonds), as showed in
Figure 3. Spectra data of IR, EI-MS, 'HNMR and 13C-NN{R. were fairly accorded

with

experimental results reported in

literature (gamma-guaiene).
3.2 The affect alpha-guaiene (CID519743)
and gamma-guaiene (CID94275) to
expression COX-I dan COX-2 on preosteoblast cell.
Expression of COX-I and COX-2 in the
pre-osteoblast cells in normal group (COX-I
ICOX-2 + FITC), LPS group (LPS induction
of 10 mg I mL + COX-I I COX-Z + FITC),
fraction alpha-guaiene
Soup
(CID519743) and fraction gamma-guaiene
(CD94275) with concentration of 20160/180
pM + COX-I I COX-? + FITC), and a group
fraction alpha-guaiene
LPS
(CID519743) and fraction gamma-guaiene
{CD94275) with concentration of 2U 6Al
180 pM + COX-I/ COX-2 + FITC)) were
incubated for 24 hours by observation using
Confocal Single Laser Microscope (CSLM).
Analysis of differences all groups in fraction
alpha-guaien and fraction gamma-guaiene,
as showed in Figure 4 and 5.

a

of

of

+

The fraction-g: o-patchoulene (16.84%), o-bulnesene (21,O4o/o)' and opatchouli alcohol

(6 1,76olo)

?

it

The-fraction-8: s-patchoulene(2,73o/o), B-caryophylene (5,57o./o)' c'
bulneaene 18,2,12o/nl, gemacrene-A (1,98%), and q-patchouli alcohol

I

B

(7,620/o)

I
I

t

_.,i

, .r

Thc fraetion-7: seychellene (2,03%), o-patchoulene (5,57olo, p-patchoulene
(3,7Oo/"1,

7

!-aryophylene (12,38?o), and o-bulnesene (76,31 7o)

The fraction-G: alpha guaiene

(6,710/o), seychellene (14,23%1, opatchoulene (23,01 oA), p-patchoulene (8,68%), B-caryophylene 18,76o/0)'

6

and o-bulneaone (38,62%)

I

rl

i[i

The fraction-S: o€urjunene

(O,860/o),

o-guaiene (21,O2o/o), seychellene

(25,05%), o-patchoulene (28,O4V'), p-patchoulene (7,O6%), 9-

5
-J

4

lli

caryophylenen (1,6Oq/"), o-bulnesene (10,5070), and q-patchouli alcohol

!lit
:t; s,

(2,22o/o)
l.-

The fractlon{: unknown-l {4,35%r, unknown-2 (1,44o/o), o€urjunene

(5,49olo), q-guaiene (32,15o/"), seychgllene (1O,71%), o-patchoulene
4%), p-patchoulene (2,460/o), p-caryophylene (2,51 o/o), q-bulnesene

i

{9,1

,-J','-j,

(1

u

9,98%), gemacrene-A(7,17Yo) and o-patchouli alcohol (4,60%)

I

The ftactlon-3: unknown-1 (11,22o/"), unknown-z (6,13o6), q€uriunene
(16,06%),