Peningkatan kualitas nutrisi silase berbahan baku singkong varietas pahit dengan enzim cairan rumen dan bakteri Leuconostoc mesenteroides sebagai pakan ternak unggas
PENINGKATAN KUALITAS NUTRISI SILASE BERBAHAN BAKU
SINGKONG VARIETAS PAHIT DENGAN ENZIM CAIRAN
RUMEN DAN BAKTERI Leuconostoc mesenteroides
SEBAGAI PAKAN TERNAK UNGGAS
SOFIA SANDI
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2010
PERNYATAAN MENGENAI DISERTASI DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa disertasi Peningkatan Kualitas Nutrisi
Silase Berbahan Baku Singkong Varietas Pahit dengan Enzim Cairan Rumen dan
Bakteri Leuconostoc mesenteroides sebagai Pakan Ternak Unggas adalah karya
saya sendiri dengan arahan dari komisi pembimbing dan belum diajukan dalam
bentuk apa pun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau kutipan dari karya yang diterbitkan maupun tidak diterbitkan dari penulis lain
telah disebutkan dalam teks dan dicantumkan dalam Daftar Pustaka dibagian akhir
disertasi ini.
Bogor, Agustus 2010
Sofia Sandi
NRP. D061040071
ABSTRACT
SOFIA SANDI. Nutrient Quality Improvement of Bitter Variety Cassava Silage
with Rumen Fluid Enzyme and Leuconostoc mesenteroides as Poultry Feed.
Advisor : ERIKA BUDIARTI LACONI, ASEP SUDARMAN, DJUMALI
MANGUNWIJAJA and KOMANG G. WIRYAWAN
The objective of this research was to improve nutrient quality of silage
with cassava as main ingredient by rumen fluid enzyme and Leuconostoc
mesenteroides as additive in ensilage process for male duck feed. The first
experiment was Leuconostoc mesenteroides bacterial isolation and identification
from cassava tuber. There were 4 selected isolates of Leuconostoc mesenteroides
(A, B, C, and D) that have ability to reduce cyanide in cassava tuber. C isolate
resulted the lowest value of cyanide and then was used for further trials. The
second experiment was the application of rumen fluid crude enzyme on cassava
substrates. The experiment used Completely Randomized Design with 15 cassava
combination and 3 replicates i.e. tuber (U), Leaf (D), Peel (K), Onggok (O),
tuber+onggok (UO), peel+tuber (KU), leaf+tuber (DU), leaf+onggok (DO),
leaf+peel (DK), peel+onggok (KO), peel+leaf+onggok (KDO), leaf+tuber+peel
(DUK), peel+tuber+onggok (KUO) leaf+tuber+onggok (DUO) and
Peel+leaf+tuber+onggok (KDUO). Result of the second experiment showed that
the crude enzyme of rumen fluid did not significantly affect dry matter losses
(0.96-2.08%), but it had significant effect on crude fiber decrease (8.61-17.83%)
and total sugar increase (15.19-29.52%). The third experiment used the same
treatment as second experiment for nutrient analyses before and after ensilage
processing. Result of the third experiment showed that the temperature in
ensilage process ranged between 26-300C, acid and fermented smelling, colour
changing (cream, brown and yellow green), Ensilage process had significant
(P
SINGKONG VARIETAS PAHIT DENGAN ENZIM CAIRAN
RUMEN DAN BAKTERI Leuconostoc mesenteroides
SEBAGAI PAKAN TERNAK UNGGAS
SOFIA SANDI
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2010
PERNYATAAN MENGENAI DISERTASI DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa disertasi Peningkatan Kualitas Nutrisi
Silase Berbahan Baku Singkong Varietas Pahit dengan Enzim Cairan Rumen dan
Bakteri Leuconostoc mesenteroides sebagai Pakan Ternak Unggas adalah karya
saya sendiri dengan arahan dari komisi pembimbing dan belum diajukan dalam
bentuk apa pun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau kutipan dari karya yang diterbitkan maupun tidak diterbitkan dari penulis lain
telah disebutkan dalam teks dan dicantumkan dalam Daftar Pustaka dibagian akhir
disertasi ini.
Bogor, Agustus 2010
Sofia Sandi
NRP. D061040071
ABSTRACT
SOFIA SANDI. Nutrient Quality Improvement of Bitter Variety Cassava Silage
with Rumen Fluid Enzyme and Leuconostoc mesenteroides as Poultry Feed.
Advisor : ERIKA BUDIARTI LACONI, ASEP SUDARMAN, DJUMALI
MANGUNWIJAJA and KOMANG G. WIRYAWAN
The objective of this research was to improve nutrient quality of silage
with cassava as main ingredient by rumen fluid enzyme and Leuconostoc
mesenteroides as additive in ensilage process for male duck feed. The first
experiment was Leuconostoc mesenteroides bacterial isolation and identification
from cassava tuber. There were 4 selected isolates of Leuconostoc mesenteroides
(A, B, C, and D) that have ability to reduce cyanide in cassava tuber. C isolate
resulted the lowest value of cyanide and then was used for further trials. The
second experiment was the application of rumen fluid crude enzyme on cassava
substrates. The experiment used Completely Randomized Design with 15 cassava
combination and 3 replicates i.e. tuber (U), Leaf (D), Peel (K), Onggok (O),
tuber+onggok (UO), peel+tuber (KU), leaf+tuber (DU), leaf+onggok (DO),
leaf+peel (DK), peel+onggok (KO), peel+leaf+onggok (KDO), leaf+tuber+peel
(DUK), peel+tuber+onggok (KUO) leaf+tuber+onggok (DUO) and
Peel+leaf+tuber+onggok (KDUO). Result of the second experiment showed that
the crude enzyme of rumen fluid did not significantly affect dry matter losses
(0.96-2.08%), but it had significant effect on crude fiber decrease (8.61-17.83%)
and total sugar increase (15.19-29.52%). The third experiment used the same
treatment as second experiment for nutrient analyses before and after ensilage
processing. Result of the third experiment showed that the temperature in
ensilage process ranged between 26-300C, acid and fermented smelling, colour
changing (cream, brown and yellow green), Ensilage process had significant
(P