Efektivitas Pemberian Silase dan Probiotik Terhadap Ekologi Rumen Sapi Peranakan Ongol Menggunakan Analisis Terminal Restriction Fragment Length Polymorphism (T-RFLP)
i
EFEKTIVITAS PEMBERIAN SILASE DAN PROBIOTIK TERHADAP
EKOLOGI RUMEN SAPI PERANAKAN ONGOL MENGGUNAKAN
ANALISIS TERMINAL RESTRICTION FRAGMENT LENGTH
POLYMORPHISM (T-RFLP)
RONI RIDWAN
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2009
ii
PERNYATAAN MENGENAI TESIS DAN SUMBER INFORMASI
Dengan ini saya menyatakan bahwa tesis EFEKTIVITAS PEMBERIAN
SILASE DAN PROBIOTIK TERHADAP EKOLOGI RUMEN SAPI
PERANAKAN ONGOL MENGGUNAKAN ANALISIS TERMINAL
RESTRICTION
FRAGMENT
LENGTH
POLYMORPHISM
(T-RFLP) adalah karya saya sendiri dan belum diajukan dalam bentuk apa
pun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau dikutip dari karya yang diterbitkan maupun tidak diterbitkan dari
penulis lain telah disebutkan dalam teks dan dicantumkan dalam Daftar
Pustaka di bagian akhir tesis ini.
Bogor, Januari 2009
Roni Ridwan
NRP. P052060051
iii
ABSTRACT
RONI RIDWAN. Effectivity of Silage and Probiotic on Rumen Ecology of
Peranakan Ongole Cattle by Terminal Restriction Fragment Length
Polymorphism (T-RFLP) Analysis. Under direction of SRI BUDIARTI and
ACHMAD DINOTO.
Silages and Probiotics additives were used for improvement of
cattle productivity. Key of effectivity of silage and probiotics was changed
observation direct or indirect roles on rumen ecology. The objective of this
study was to examine the effects of silage and probiotics on ruminal
ecosystems in vivo using molecular approach. Terminal-restriction
fragment length polymorphism (T-RFLP) analysis was used to detect
changes of ecology communities based on 16S ribosomal deoxyribonucleic
acid (16S rDNA).
Two rumen canulated PO cattle were fed several diet ie; (R0) basal
diet dry matter basis (Pennisetum purpureum 70% and commercial
concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus
plantarum BTCC570), (R2) silage + probiotics (Leuconostoc sp. BTCC531),
(R3) Basal diet + probiotics (Leuconostoc sp. BTCC531). Digesta samples
were colected 3 h after feeding for cellulolytic activities (DNS method), pH,
parsial volatile fatty acids measurements (Gas Chromatography method),
and T-RFLP analysis. T-RFLP analysis was performed using the 16S rDNA
amplified from each sample. The lengths of the terminal restriction
fragments were analysed after digestion with HhaI, HaeIII and MspI.
Result showed that effectivities of silage and probiotics given
together of index Smith and Wilson evenness applied to T-RFLP ecology
data (Evar) 0.89±0.04 was the highest. It was significantly (p
EFEKTIVITAS PEMBERIAN SILASE DAN PROBIOTIK TERHADAP
EKOLOGI RUMEN SAPI PERANAKAN ONGOL MENGGUNAKAN
ANALISIS TERMINAL RESTRICTION FRAGMENT LENGTH
POLYMORPHISM (T-RFLP)
RONI RIDWAN
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2009
ii
PERNYATAAN MENGENAI TESIS DAN SUMBER INFORMASI
Dengan ini saya menyatakan bahwa tesis EFEKTIVITAS PEMBERIAN
SILASE DAN PROBIOTIK TERHADAP EKOLOGI RUMEN SAPI
PERANAKAN ONGOL MENGGUNAKAN ANALISIS TERMINAL
RESTRICTION
FRAGMENT
LENGTH
POLYMORPHISM
(T-RFLP) adalah karya saya sendiri dan belum diajukan dalam bentuk apa
pun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau dikutip dari karya yang diterbitkan maupun tidak diterbitkan dari
penulis lain telah disebutkan dalam teks dan dicantumkan dalam Daftar
Pustaka di bagian akhir tesis ini.
Bogor, Januari 2009
Roni Ridwan
NRP. P052060051
iii
ABSTRACT
RONI RIDWAN. Effectivity of Silage and Probiotic on Rumen Ecology of
Peranakan Ongole Cattle by Terminal Restriction Fragment Length
Polymorphism (T-RFLP) Analysis. Under direction of SRI BUDIARTI and
ACHMAD DINOTO.
Silages and Probiotics additives were used for improvement of
cattle productivity. Key of effectivity of silage and probiotics was changed
observation direct or indirect roles on rumen ecology. The objective of this
study was to examine the effects of silage and probiotics on ruminal
ecosystems in vivo using molecular approach. Terminal-restriction
fragment length polymorphism (T-RFLP) analysis was used to detect
changes of ecology communities based on 16S ribosomal deoxyribonucleic
acid (16S rDNA).
Two rumen canulated PO cattle were fed several diet ie; (R0) basal
diet dry matter basis (Pennisetum purpureum 70% and commercial
concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus
plantarum BTCC570), (R2) silage + probiotics (Leuconostoc sp. BTCC531),
(R3) Basal diet + probiotics (Leuconostoc sp. BTCC531). Digesta samples
were colected 3 h after feeding for cellulolytic activities (DNS method), pH,
parsial volatile fatty acids measurements (Gas Chromatography method),
and T-RFLP analysis. T-RFLP analysis was performed using the 16S rDNA
amplified from each sample. The lengths of the terminal restriction
fragments were analysed after digestion with HhaI, HaeIII and MspI.
Result showed that effectivities of silage and probiotics given
together of index Smith and Wilson evenness applied to T-RFLP ecology
data (Evar) 0.89±0.04 was the highest. It was significantly (p