RETINOIC ACID EFFECTS AND FOIIOWING EFFECTS THROUGH CHITOSAN TOWARDS PREGNANCY MICE Repository - UNAIR REPOSITORY
&
*
%
ffi
I
E
VzlZl
r
E
D
s
b
j;
E
F
l
I
r12
!
r*J
L'
L
!
'%E
\
I
B
\
l*
I
I
\
*-'''€
Surabaya, 22-23 June 2010
Tandjung Adiwinata Room
Faculty of Veterinary Medicine Airlangga University
Editor:
Pudji Srianto (lndonesia)
Mustopha B. Abubakar (Nigeria)
M. Yunus (lndonesia)
Suzanita Utama (lndonesia)
Suwarno (lndonesia)
f'M HERE Sub-component 8.2.c Performance Based Contract
Join with Faculty of Veterinary Medicine Airlangga University
)
O
2010 Airlangga University Press
AUP 600/34.379lll.l0-B1E
AII rights reserved. This book or any part there of may not be reprinted or reproduced
or utilised in any form or by an electronic, mechanical, or other means, including photocopying
and recording or in any inJormation storage in retrieval system, without permission in writing
from the publisher.
First
print
-
2010
Publisher:
Pusat Penerbitan dan Percetakan UnaA (AUP)
Campus C Unair, Mulyorejo Surabaya Indonesia 60115
Phone. +52 31 5992246,5992247 Fax. +62315992248
E-mail: aupsbya.drad.net.id
Printed by
r
Pusat Penerbitan dan Percetakan Unair (AUP)
(143/10.1o/AUP-B1E)
Library of N ational : Cataloging-in-Publication Data
PRO
Proceeding Intemational Seminar: Strategies for the Control & Prevention
of Zoonotic Diseases (1tabaya,22 23 June 2010. Tandjung Adiwinata Room,
Faolty of Veterinary Medicine Airlartga University/Editor: Pudii Srianto ... [et al.]
- l't Print-
xvii,
Surabaya: Airlangga University Press, 2010
, 29,7 crrr
151 p.; ilus.; 21
Bibliography
ISBN 978-602-8967-00.6
I. Title
1. Animal - Diseases
2. Domestic Animals
- Diseases
636.089 691
10 11 12 13 14
MEMBER OF
IKAPI 0014TV95
1987 654321
tlST OF CONTENTS
Report of Organizing Committee
Wellcome Speech from Dean Faculty of Veterinary Medicine Airlangga University...........
Organizing Committee......
vll
ix
xi
Program khedule
xiii
Welcome Message from Rector of Airlangga University
MAIN SPEAKER
Characteristics of Rabies Virus in Indonesia
Suwamo ..............
1
Avian and Swine Flu Outbreal: Surabaya Experience
6
I aksmi Wulandari..........................--.
Biology and Characterization of Boma Disease Virus In{ection
Fedik A. Rantam, Live Bode, Hanns Ludwig.
Adjuvant For Zoonotic Diseases, Model Specificity?
R. Vialle, L. Dupuis, S. Deville, F. Bertrand and
J.
9
Aucouturier............
Avian Inlluenza in Nigeria: Present Situation and Assessment of Prevention and
Control Paradigms
Mustapha B. Abubakar, Aini Ideris, S.S. Baba" A.R Omar and A.D. Ely'uguda.....
12
13
Brucellosis in Malaysia
M. Zamri-Saad.....
15
Parasitic Zoonoses: Immunocompromise and Macroenvironment
Yoes Pri,atna Dachlan.........................
17
Epidemiology of Rabies in Indonesia
22
Soe8iarto...............
FREE PAPER
The First Case of Rabies in Bali was Diagnosed by Using Polymerase Chain Reaction
Faizah, Dibia, I.N, Suryadinata, L.M.F......... ... ........
27
The Role of Gamma-interferon (IFN-'y) in Host Defense Against Toxoplasmic Encephalitis
30
Nurina Hasanatuludhhiyah......
Salmonellosis Infection in Molting Phase with Dietary Feed Carried Out Method in the Ducks
Erma Safitri, Eko Prasetyo Kuncoro, Husni Anwar and Pudii Srianto..........
34
Epidemiology Study of Avian Influenza (H5N1) Virus: Distribution in Day Old Chick (DOC)
Setyawati S, E. Handharyani, R.D. Soeioedono and B Sumiarto ....
40
The Processing Effect of Flour Worms with Temperature and Type Worms Variation to
Inhibit the Growth of Salmonella Typhi rn vilro
Bayyinatul Muchctaromah
Molecular Diagnosis of Toksoplasmosis Based on toxoplasma gondii Repetitive DNA Fragment
Dyah Ayu Oktavianie, Sumartono, Wayan T. Artama
Detection of the
G
ene for Staphylococcus Aureus Enterotoxin C from Raw
Mustofa Helmi Effendi
l'MHERE Sub-component 8.2.c. Performance Based Conttact
Joint with Faculty of Veterinary Medicine Airlangga tJniversity
43
48
Milk Samples By PCR
53
XY
The Prevalence of Toxoplasmosis on Beef Cattles in Pegirian Slaughterhause, Surabaya
Rochiman Sasmita, Kusriningrum Rochiman $ Setya Budi and Ilyas Wiradirga..
56
Molecular Characterization of Pfcrt Gene for Detection Chloroquine Antimalarial Drug
Resistance at Clinic Isolates Plasmodium Falcifarum
Lilik Maslachah, Eddy Soewandojo, Mufasiriry Yuni Priyandani,
Loeki Enggar Fitri, Didi Candradikusuma.....................
59
Good Veterinary Vaccines for Controlong and Prevention of Zoonotic Diseases
Yuni Yupiana, Ida Lestari Soedijar, Bahruddin Syahroni ....
53
Screening of Bendu Duku for Antiproliferation of Myeloma Cell
Lazuardi M., Nuraini F., Ratna SM, Roostantia I. .................
65
Diphyllobothrium Marsozi Larvae in Frog Flash Cause of Sparganosis in Human
R. Heru Prasetyo and Erma Safitri ....
69
Protection Mechanism of Serotherapy using Egg Yolk Derived Anti-Haemaglutinin
Avian Influenza Virus Antibody In Chicken
Suryo Kuncorojakti and Suwamo ...
77
Safety and Immunoenhancing Effe ct of Chlorella Dietary Supplement in Layer
Undergoing Avian Influenza Vaccination
Y Dhamayanti and H. Eliyani......
/3
The Influence of Spirulina Platensis on Feed Efficienry Ratio of H5N1 Vaccinated in Layer Hen
Widya PL, Suwamo, H. Setyono, L. Mirni, Y. Dhamayanti, A.B. Yulianto ..........................
77
Detection of Anti-Neuraminidase Antibodies Avian Inlluenza Viruses Subtype H5N1
in Serum and Egg Yolk of Chicken using lndirect Elisa
Retno Wulan Handayani, Suwarno......
80
Observasi Strongyloides pada Tikus Liar
Kusmartisnawati, Heny Arwati, M Yasin FN, Yoes Pri,atna Dachlan..
L,/
82
Study on Avian Influenza on Waterfowl in Surabaya Seashore as a Stopover Migrant Birds
E. Djoko Poetranto
85
The Effect of Prostaglandin F2c on Sperm Motility in Ettawah Cross Bred Goat Semen
Dilute with Citrate Yolk or Skim Milk
Herawati..............
89
Reproductive Tract lnfections Impact on Quality Spermatozoa
Sukarjati, Sudlarwo, Doddy M Soebadi, Aucky H intin9.................
9L
Retinoic Acid Effects and Following Effects Through Chitosan Towards Pregnanry Mice
Bambang Poemomo S., Win Darmanto, lda Prijatni, Teresia Retno Puspitadewi...................
Treatment of Ovary Hypofunction in Friesian Holstain Cows Using Exctracted Local Indonesian
Pregnant Mare Serum Gonadotrophin (PMSG Gonaplas Airlangga Univ.)
Hermadi HA and Mahaputra L. ........................
100
'103
Effect of Inhibin B on Sperm Quality: a Development of Peptide Male Hormonal Contraception
Aulanni'am, Catot Ciptadi, Chanif Mahdi, and Muslim Akmal ...........................
707
Monoclonal Antibodies to Bovine Zonna Pellucida 3 Deglycosylated bZP3dG)
for Candidate Woman Immunocontraceptive Vaccine
Sutiman B. Sumitro, Aulanni'am, Ciptadi. C., Soewarto. S.........................................
110
Abnormaly Variation of Development Nile Tilapia Larvae Incubated in Various Salinity
Juni Triastuti
113
KJIY (Koi Herpes Wrus) Transmissions Analysis Through a Finding Host from Several Kind
of Freshwater Fish
L. Sulmartiwi, T. Rahmawati, H. Suprapto, Agustono.....................
116
xYt
I
lnternational Seminar
New Strategies lor the Control & Prevention of Zoonotic Diseases
Role an Endothelial Adhesin in Atherogenesis and Rupture of Plaques Atherosclerotic
Sri Murwani
1.19
Prospect Perform of Monoclonal Antibody Visceral Larva Toxocara spp (MAB TVI-3 P97)
Kit Against Human Creeping Eruption the Outbreak of Feather Mites of Poultry
Yunus, M.
723
as Serodiagnostic
The Outbreak of Feather Mites of Poultry
1_27
Sero-Surveillance of Influenza Virus H5N1 on Avian (Chicken and Duck)
in Several Traditional Markets of Surabaya
Yunus, M., Emawati, R., and Rachmar; M.H
129
Antigenicity, Sensitivity AMD Spesificity of CathepsinJ Protein from Excretory-secretory
Material of Fasciola Gigantica for Distomatosis Diagnosis in Cattle Sera by using
Indirect-Elisa Technique
Kusnoto
133
Preventing the Spread of HIV/AIDS using
a Cultural Approach
Herin Setianingsih and Myrtati D. Artaria
739
The Influence of the Dry and Canned Commercial Cat Food Toward Urine Volume,
Water Intake Amount, and Kidneys Function Condition of Male Cat
Lita Rakhma Yustinasari, Nusdianto Triakoso and Sri Mumpuni S.
1,43
Specificity Test of Kit Ligand Protein Isolated from Bovine Mature Oocyte
Widjiati, Theresia Audita Guretti, Sri Pantja Madyawati, Hana Eliyani
745
...
151
IMHERE Sub component B.2.c. Performance Based Contract
Joint with Faculty of Veterinary l\,4edicine Airlangga University
I xv
RETINOIC ACID EFFECTS AND FOIIOWING EFFECTS THROUGH
CHITOSAN TOWAR.DS PR.EGNANCY MICE
Bambang Poemomo S., Win Darmanto, Ida Priiatni, Teresia Retno PusPitadewi
Faolty of Veterinary Medicine, Airlang8a University
ABSTRACT
lipid sotuble Ionn ol oitamin A The function
an essetltiul tutrienl for fepfoduction ald growth. Retinoic acid is one amonS
so that a.n ex.essiue dosage will cause
lh;body,
to-excrct?
lime
nkr,
grneln
ol
lrom
of retinoic acid is regulolor and transc.ription
t abnonnatities
it
particutarly,
in
mothers,
of
.nuy .res
7r;;;;;;;"i";;,,:;;;tty in *e st 0.05). The result of normality test indicated that all
samples in the field of reproductive outcome mainly
the number of placenta implantation and fetal body
weight had normal distribution (P > 0.05), whether
living delivery dead delivery and resorpted embryo
showed skewed distribution (P < 0.05). The analysis
ofhomogeneity test indicated that number of Placenta
implantation and fetal body weight had homogenous
variance (P > 0.05).
The result of independent two sample t-test
showed that the mean of placenta implantation had
significantly difference on 0.366 (P > 0.05), and the
mean of fetal bodyweight had significantly difference
on 0.711 (P > 0.05). Both placenta implantation and
fetal bodyweight of the test indicated that induced
retinoic acid oral administration single dose of
60 mg/Kg body weight provided no significantly
Table
1. Total abnormality morphology of mice fetuses
Groups
Total
Pregnant
Mice
Palato
Schysis
nelia
Control
10
0
0
Treatment
13
Poco-
Abnormalitv Morphology (9i,)
Syndac- Agenesis
Extra
Micro
Kinky Ectro
Others
Tail
Melia
tily
Dactil
y
Budding
Tail
0
0
0
0
79,4 60,7 45,6 32,4 22,1 22,7
0
20,6
0
77,6
0
47
Source: Puspitadewi, T.R., Darmanto W., and Poemomo B. 2008.
I MHERE Sub-component B.2 c Performance Based Contract
Joint with Faculty of Veterinary Medicine Airlangga University
IOI
Free Paper
difference on the development malformation
moroholosv. Therefore, observation for the delay
or"iil.utlir, founded significantly d iffe ren ce
f,E
like sacrum' bone
of both' forelimb
distal
part
part caudal, phalanges
and hind limb.
(P < 0.05), especially on the bones
I
-"4
*
/
1
t"
REFERENCES
CEI{TRI.IM
\
AiC
Control
US
Treatment
Fiture 1. Vertebrae column on delivery fetal mice
Centrum will develoPed on the heatment grouP'
instead none on the control Source: Prijatni' I"
Darmanto W., and Poernomo 8 2008'
The result of the second research shows at the
three different chitosan groups are not significantly
r02l
Pri,atni, I., Darmanto W., arrd Poemomo B 2008 Pengaruh
'khiiosalr terhadap ketainan rangka ianin mencit (Mris
musculus) akibatisam retinoat Tesis (tida} diterbitkan)
Program Pascasariana. Falultas Kedokteran Universitas
Airlangga. Surabaya.
8 2008
Pusoitadeiryl, T.R., Dirmanto W and Poemomo
mencit
indul
pada
diberilan
vang
retinoat
u'uif"k
terhadaP
10
hari
(Mus musculus\ umur iebunthgan
hasil reproduksi dan kelainan bawaan ekstemal ianin'
Fakultas
Tesis (tiaak diterbitkan). Progam Pascasarjana
Surabaya'
Airlantga
Universitas
Kedokteran.
lnternational Semirlar
Diseas6
New Strategies for the Control & Prevention ol Zoonotic
*
%
ffi
I
E
VzlZl
r
E
D
s
b
j;
E
F
l
I
r12
!
r*J
L'
L
!
'%E
\
I
B
\
l*
I
I
\
*-'''€
Surabaya, 22-23 June 2010
Tandjung Adiwinata Room
Faculty of Veterinary Medicine Airlangga University
Editor:
Pudji Srianto (lndonesia)
Mustopha B. Abubakar (Nigeria)
M. Yunus (lndonesia)
Suzanita Utama (lndonesia)
Suwarno (lndonesia)
f'M HERE Sub-component 8.2.c Performance Based Contract
Join with Faculty of Veterinary Medicine Airlangga University
)
O
2010 Airlangga University Press
AUP 600/34.379lll.l0-B1E
AII rights reserved. This book or any part there of may not be reprinted or reproduced
or utilised in any form or by an electronic, mechanical, or other means, including photocopying
and recording or in any inJormation storage in retrieval system, without permission in writing
from the publisher.
First
-
2010
Publisher:
Pusat Penerbitan dan Percetakan UnaA (AUP)
Campus C Unair, Mulyorejo Surabaya Indonesia 60115
Phone. +52 31 5992246,5992247 Fax. +62315992248
E-mail: aupsbya.drad.net.id
Printed by
r
Pusat Penerbitan dan Percetakan Unair (AUP)
(143/10.1o/AUP-B1E)
Library of N ational : Cataloging-in-Publication Data
PRO
Proceeding Intemational Seminar: Strategies for the Control & Prevention
of Zoonotic Diseases (1tabaya,22 23 June 2010. Tandjung Adiwinata Room,
Faolty of Veterinary Medicine Airlartga University/Editor: Pudii Srianto ... [et al.]
- l't Print-
xvii,
Surabaya: Airlangga University Press, 2010
, 29,7 crrr
151 p.; ilus.; 21
Bibliography
ISBN 978-602-8967-00.6
I. Title
1. Animal - Diseases
2. Domestic Animals
- Diseases
636.089 691
10 11 12 13 14
MEMBER OF
IKAPI 0014TV95
1987 654321
tlST OF CONTENTS
Report of Organizing Committee
Wellcome Speech from Dean Faculty of Veterinary Medicine Airlangga University...........
Organizing Committee......
vll
ix
xi
Program khedule
xiii
Welcome Message from Rector of Airlangga University
MAIN SPEAKER
Characteristics of Rabies Virus in Indonesia
Suwamo ..............
1
Avian and Swine Flu Outbreal: Surabaya Experience
6
I aksmi Wulandari..........................--.
Biology and Characterization of Boma Disease Virus In{ection
Fedik A. Rantam, Live Bode, Hanns Ludwig.
Adjuvant For Zoonotic Diseases, Model Specificity?
R. Vialle, L. Dupuis, S. Deville, F. Bertrand and
J.
9
Aucouturier............
Avian Inlluenza in Nigeria: Present Situation and Assessment of Prevention and
Control Paradigms
Mustapha B. Abubakar, Aini Ideris, S.S. Baba" A.R Omar and A.D. Ely'uguda.....
12
13
Brucellosis in Malaysia
M. Zamri-Saad.....
15
Parasitic Zoonoses: Immunocompromise and Macroenvironment
Yoes Pri,atna Dachlan.........................
17
Epidemiology of Rabies in Indonesia
22
Soe8iarto...............
FREE PAPER
The First Case of Rabies in Bali was Diagnosed by Using Polymerase Chain Reaction
Faizah, Dibia, I.N, Suryadinata, L.M.F......... ... ........
27
The Role of Gamma-interferon (IFN-'y) in Host Defense Against Toxoplasmic Encephalitis
30
Nurina Hasanatuludhhiyah......
Salmonellosis Infection in Molting Phase with Dietary Feed Carried Out Method in the Ducks
Erma Safitri, Eko Prasetyo Kuncoro, Husni Anwar and Pudii Srianto..........
34
Epidemiology Study of Avian Influenza (H5N1) Virus: Distribution in Day Old Chick (DOC)
Setyawati S, E. Handharyani, R.D. Soeioedono and B Sumiarto ....
40
The Processing Effect of Flour Worms with Temperature and Type Worms Variation to
Inhibit the Growth of Salmonella Typhi rn vilro
Bayyinatul Muchctaromah
Molecular Diagnosis of Toksoplasmosis Based on toxoplasma gondii Repetitive DNA Fragment
Dyah Ayu Oktavianie, Sumartono, Wayan T. Artama
Detection of the
G
ene for Staphylococcus Aureus Enterotoxin C from Raw
Mustofa Helmi Effendi
l'MHERE Sub-component 8.2.c. Performance Based Conttact
Joint with Faculty of Veterinary Medicine Airlangga tJniversity
43
48
Milk Samples By PCR
53
XY
The Prevalence of Toxoplasmosis on Beef Cattles in Pegirian Slaughterhause, Surabaya
Rochiman Sasmita, Kusriningrum Rochiman $ Setya Budi and Ilyas Wiradirga..
56
Molecular Characterization of Pfcrt Gene for Detection Chloroquine Antimalarial Drug
Resistance at Clinic Isolates Plasmodium Falcifarum
Lilik Maslachah, Eddy Soewandojo, Mufasiriry Yuni Priyandani,
Loeki Enggar Fitri, Didi Candradikusuma.....................
59
Good Veterinary Vaccines for Controlong and Prevention of Zoonotic Diseases
Yuni Yupiana, Ida Lestari Soedijar, Bahruddin Syahroni ....
53
Screening of Bendu Duku for Antiproliferation of Myeloma Cell
Lazuardi M., Nuraini F., Ratna SM, Roostantia I. .................
65
Diphyllobothrium Marsozi Larvae in Frog Flash Cause of Sparganosis in Human
R. Heru Prasetyo and Erma Safitri ....
69
Protection Mechanism of Serotherapy using Egg Yolk Derived Anti-Haemaglutinin
Avian Influenza Virus Antibody In Chicken
Suryo Kuncorojakti and Suwamo ...
77
Safety and Immunoenhancing Effe ct of Chlorella Dietary Supplement in Layer
Undergoing Avian Influenza Vaccination
Y Dhamayanti and H. Eliyani......
/3
The Influence of Spirulina Platensis on Feed Efficienry Ratio of H5N1 Vaccinated in Layer Hen
Widya PL, Suwamo, H. Setyono, L. Mirni, Y. Dhamayanti, A.B. Yulianto ..........................
77
Detection of Anti-Neuraminidase Antibodies Avian Inlluenza Viruses Subtype H5N1
in Serum and Egg Yolk of Chicken using lndirect Elisa
Retno Wulan Handayani, Suwarno......
80
Observasi Strongyloides pada Tikus Liar
Kusmartisnawati, Heny Arwati, M Yasin FN, Yoes Pri,atna Dachlan..
L,/
82
Study on Avian Influenza on Waterfowl in Surabaya Seashore as a Stopover Migrant Birds
E. Djoko Poetranto
85
The Effect of Prostaglandin F2c on Sperm Motility in Ettawah Cross Bred Goat Semen
Dilute with Citrate Yolk or Skim Milk
Herawati..............
89
Reproductive Tract lnfections Impact on Quality Spermatozoa
Sukarjati, Sudlarwo, Doddy M Soebadi, Aucky H intin9.................
9L
Retinoic Acid Effects and Following Effects Through Chitosan Towards Pregnanry Mice
Bambang Poemomo S., Win Darmanto, lda Prijatni, Teresia Retno Puspitadewi...................
Treatment of Ovary Hypofunction in Friesian Holstain Cows Using Exctracted Local Indonesian
Pregnant Mare Serum Gonadotrophin (PMSG Gonaplas Airlangga Univ.)
Hermadi HA and Mahaputra L. ........................
100
'103
Effect of Inhibin B on Sperm Quality: a Development of Peptide Male Hormonal Contraception
Aulanni'am, Catot Ciptadi, Chanif Mahdi, and Muslim Akmal ...........................
707
Monoclonal Antibodies to Bovine Zonna Pellucida 3 Deglycosylated bZP3dG)
for Candidate Woman Immunocontraceptive Vaccine
Sutiman B. Sumitro, Aulanni'am, Ciptadi. C., Soewarto. S.........................................
110
Abnormaly Variation of Development Nile Tilapia Larvae Incubated in Various Salinity
Juni Triastuti
113
KJIY (Koi Herpes Wrus) Transmissions Analysis Through a Finding Host from Several Kind
of Freshwater Fish
L. Sulmartiwi, T. Rahmawati, H. Suprapto, Agustono.....................
116
xYt
I
lnternational Seminar
New Strategies lor the Control & Prevention of Zoonotic Diseases
Role an Endothelial Adhesin in Atherogenesis and Rupture of Plaques Atherosclerotic
Sri Murwani
1.19
Prospect Perform of Monoclonal Antibody Visceral Larva Toxocara spp (MAB TVI-3 P97)
Kit Against Human Creeping Eruption the Outbreak of Feather Mites of Poultry
Yunus, M.
723
as Serodiagnostic
The Outbreak of Feather Mites of Poultry
1_27
Sero-Surveillance of Influenza Virus H5N1 on Avian (Chicken and Duck)
in Several Traditional Markets of Surabaya
Yunus, M., Emawati, R., and Rachmar; M.H
129
Antigenicity, Sensitivity AMD Spesificity of CathepsinJ Protein from Excretory-secretory
Material of Fasciola Gigantica for Distomatosis Diagnosis in Cattle Sera by using
Indirect-Elisa Technique
Kusnoto
133
Preventing the Spread of HIV/AIDS using
a Cultural Approach
Herin Setianingsih and Myrtati D. Artaria
739
The Influence of the Dry and Canned Commercial Cat Food Toward Urine Volume,
Water Intake Amount, and Kidneys Function Condition of Male Cat
Lita Rakhma Yustinasari, Nusdianto Triakoso and Sri Mumpuni S.
1,43
Specificity Test of Kit Ligand Protein Isolated from Bovine Mature Oocyte
Widjiati, Theresia Audita Guretti, Sri Pantja Madyawati, Hana Eliyani
745
...
151
IMHERE Sub component B.2.c. Performance Based Contract
Joint with Faculty of Veterinary l\,4edicine Airlangga University
I xv
RETINOIC ACID EFFECTS AND FOIIOWING EFFECTS THROUGH
CHITOSAN TOWAR.DS PR.EGNANCY MICE
Bambang Poemomo S., Win Darmanto, Ida Priiatni, Teresia Retno PusPitadewi
Faolty of Veterinary Medicine, Airlang8a University
ABSTRACT
lipid sotuble Ionn ol oitamin A The function
an essetltiul tutrienl for fepfoduction ald growth. Retinoic acid is one amonS
so that a.n ex.essiue dosage will cause
lh;body,
to-excrct?
lime
nkr,
grneln
ol
lrom
of retinoic acid is regulolor and transc.ription
t abnonnatities
it
particutarly,
in
mothers,
of
.nuy .res
7r;;;;;;;"i";;,,:;;;tty in *e st 0.05). The result of normality test indicated that all
samples in the field of reproductive outcome mainly
the number of placenta implantation and fetal body
weight had normal distribution (P > 0.05), whether
living delivery dead delivery and resorpted embryo
showed skewed distribution (P < 0.05). The analysis
ofhomogeneity test indicated that number of Placenta
implantation and fetal body weight had homogenous
variance (P > 0.05).
The result of independent two sample t-test
showed that the mean of placenta implantation had
significantly difference on 0.366 (P > 0.05), and the
mean of fetal bodyweight had significantly difference
on 0.711 (P > 0.05). Both placenta implantation and
fetal bodyweight of the test indicated that induced
retinoic acid oral administration single dose of
60 mg/Kg body weight provided no significantly
Table
1. Total abnormality morphology of mice fetuses
Groups
Total
Pregnant
Mice
Palato
Schysis
nelia
Control
10
0
0
Treatment
13
Poco-
Abnormalitv Morphology (9i,)
Syndac- Agenesis
Extra
Micro
Kinky Ectro
Others
Tail
Melia
tily
Dactil
y
Budding
Tail
0
0
0
0
79,4 60,7 45,6 32,4 22,1 22,7
0
20,6
0
77,6
0
47
Source: Puspitadewi, T.R., Darmanto W., and Poemomo B. 2008.
I MHERE Sub-component B.2 c Performance Based Contract
Joint with Faculty of Veterinary Medicine Airlangga University
IOI
Free Paper
difference on the development malformation
moroholosv. Therefore, observation for the delay
or"iil.utlir, founded significantly d iffe ren ce
f,E
like sacrum' bone
of both' forelimb
distal
part
part caudal, phalanges
and hind limb.
(P < 0.05), especially on the bones
I
-"4
*
/
1
t"
REFERENCES
CEI{TRI.IM
\
AiC
Control
US
Treatment
Fiture 1. Vertebrae column on delivery fetal mice
Centrum will develoPed on the heatment grouP'
instead none on the control Source: Prijatni' I"
Darmanto W., and Poernomo 8 2008'
The result of the second research shows at the
three different chitosan groups are not significantly
r02l
Pri,atni, I., Darmanto W., arrd Poemomo B 2008 Pengaruh
'khiiosalr terhadap ketainan rangka ianin mencit (Mris
musculus) akibatisam retinoat Tesis (tida} diterbitkan)
Program Pascasariana. Falultas Kedokteran Universitas
Airlangga. Surabaya.
8 2008
Pusoitadeiryl, T.R., Dirmanto W and Poemomo
mencit
indul
pada
diberilan
vang
retinoat
u'uif"k
terhadaP
10
hari
(Mus musculus\ umur iebunthgan
hasil reproduksi dan kelainan bawaan ekstemal ianin'
Fakultas
Tesis (tiaak diterbitkan). Progam Pascasarjana
Surabaya'
Airlantga
Universitas
Kedokteran.
lnternational Semirlar
Diseas6
New Strategies for the Control & Prevention ol Zoonotic