PEMANFAATAN KALSIUM PADA PUYUH (Coturnix coturnix japonica) DENGAN SUPLEMENTASI VITAMIN D DALAM RANSUM YANG MENGANDUNG FITASE.
V . SIMPUL A N
Suplementasi vitamin D dalam ransum yang mengandung fitase 500 FTU,
Ca 3,3%, P tersedia 0,5% pada level 500 IU dapat meningkatkan bobot dan massa
Ca kerabang.
22
DA F T A R PUST A K A
A bdel-A zeem, F. A . and A . A . El-Shafei. 2006. Effect of different levels of
cholecalciferol (vitamin D3) on performance and skeletal bone formation
of laying J apanese quail reared under hot climate region. Egyptian Poultry
Science J ournal. 26: 221-234.
A lmatsier, S. 2004. Prinsip Dasar Ilmu Gizi. PT. Gramedia Pustaka Utama,
J akarta.
A min, M., D. J usadi dan I. Mokoginta. 2011. Penggunaan enzim fitase untuk
meningkatkan ketersediaan fosfor dari sumber bahan nabati pakan dan
pertumbuhan ikan lele (Clarias sp). J urnal Saintek Perikanan. 6: 52-60.
A mrullah, I. K . 2003. Nutrisi A yam Petelur. L embaga Satu Gunungbudi, Bogor.
A nggorodi, H. R. 1995. Nutrisi A neka Ternak Unggas. PT Gramedia Pustaka,
J akarta.
A smarasari, S. A . dan E. Suprijatna. 2007. Respon pemberian pellet kunyit
(Curcuma domestica) terhadap performans produksi dan efisiensi
penggunaan protein ayam pedaging. Dalam: Prosiding Seminar Nasional
Hari Pangan Sedunia X X V II. Universitas Diponegoro. Semarang. hal.
251-256.
A stawan, M. 2009. Sehat dengan Hidangan K acang dan Biji-bijian. Penebar
Swadaya, J akarta.
A ureli, R., M. U. Faruk, I. Cechova, P. B. Pedersen, S.G. Elvig-J oergensen, F. Fru
and J . Broz. 2011. The efficacy of a novel microbial 6-phytase expressed
in Aspergillus oryzae on the performance and phosphorus utilization in
broiler chickens. International J ournal of Poultry Science. 10: 160-168.
Baruah, K ., N.P. Sahu, A .K . Pal and D. Debnath . 2004. Dietary phytase: an ideal
approach for a cost effective and low-polluting aqua feed. NA GA , World
Fish Center Quarterly. 27: 15-19.
Blakely, J . dan D. H. Bade. 1998. Ilmu Peternakan. Edisi ke Empat.
(Diterjemahkan oleh: B. Srigandono). Gadjah Mada University Press.
Y ogyakarta.
Bradfield, J . R. G. 1951. Radiographic studies on the formation of the hen's egg
shell. J ournal of Experimental Biology. 28: 125-141.
Costa, C.H.R., S.L.T. Barreto, P.C. Gomes, L .H. Hosoda, C.A . L ipari and H.J .D.
L ima. 2011. A vailable phosphorus levels in diets for J apanese quail 45-57
weeks of age. J ournal of A nimal Science. 40: 2152-2160.
Clunies, M., D. Parks and S. L eeson. 1992. Calcium and phosporus metabolism
and eggshell formation of hens fed different amounts of calcium. Poultry
Science. 71: 482-489.
23
Edwards, H. M., J r. 1993. Dietary 1,25-dihydroxycholecalciferol supplementation
increases natural phytate phosphorus utilization in chickens. J ournal of
Nutrition. 123: 567-577.
Edwards, H. M., J r., M. A . Elliot and S. Sooncharernying. 1992. Effect of dietary
calcium on tibial dyschondroplasia. Interaction with light, cholecalciferol,
1,25-dihydroxycholecalciferol, protein, and synthetic zeolite. Poultry
Science. 71: 2041-2055.
Eklund, M., R. Mosenthin, M. Tafaj and J . Wamatu. 2006. Effects of betaine and
condensed molasses solubles on nitrogen balance and nutrient digestibility
in piglets fed diets deficient in methionine and low in compatible
osmolytes. A rchieves of A nimal Nutrition. 60: 289-300.
El-Maksoud, A . A . 2010. Effect of dietary calcium and vitamin D3 levels on egg
production and egg shell quality of hy-line brown - egg type laying hens.
Egyptian Poultry Science J ournal. 30: 1097-1120.
Etches, R. J . 1996. Reproduction in Poultry. CA B International. Wallingford.
Oxon. UK .
Ferket, P. R and A . G. Gernat. 2006. Factors that affect feed intake of meat birds:
A Review. International J ournal of Poultry Science. 5: 905-911.
Frost and Roland. 1990. Influence of vitamin D3, 1 alpha-hydroxyvitamin D3, and
1,25-dihydroxyvitamin D3 on eggshell quality, tibia strength, and various
production parameters in commercial laying hens. Poultry Science. 69:
2008-2016.
Garcia A . F. Q. M., E. M. A lice, R. A . D. Cristiane, C. O. R. Iván, K arla Paola
Picoli and M. Z. Maíra. 2013. Use of vitamin D and its metabolites in
broiler chicken feed on performance, bone parameters and meat quality.
A sian-A ustralasian J ournal of A nimal Science. 26(3): 408-415
Greiner, R., E. Haller, U. K onietzny and K .D. J any. 1997. Purification and
characterization of a phytase from Klebsiella terrigena. A rchieves
Biochemistry Biophysics. 341: 201-206.
Hartadi, H., S. Reksohadiprojo dan A . D. Tillman. 1994. T abel K omposisi Pakan
untuk Indonesia. Gadjah Mada University Press, Y ogyakarta.
Indreswari, R., H. I. Wahyuni, N. Suthama and P. W. Ristiana. 2009. Calcium
utilization for egg shell formation in laying hens due to the different
feeding regimes of morning and afternoon portion. J ournal of the
Indonesian Tropical A nimal A griculture. 34: 134-138.
K asiyati, N. K usumorini, H. Maheshwari dan W. Manalu. 2010. K ajian fisiologis
status kalsium puyuh (Coturnix coturnix japonica) setelah pemberian
cahaya monokromatik. Buletin A natomi dan Fisiologi. 18: 1-11.
K eshavarz, K . 2003. A comparison between cholecalciferol and 25-OHcholecalciferol on performance and eggshell quality of hens fed different
levels of calcium and phosporus. Poultry Science. 82: 1415-22.
24
L avelin, I., N. Meiri and M. Pines. 2000. New insight in eggshell formation.
Poultry Science. 79: 1014-1017.
L eeson, S and J . D. Summer. 2001. Feeding Programs for L aying Hens. Buletin
A SA Technical V olume PO49. A merican Soybean A ssociation.
L istiyowati, E dan K . Roospitasari. 2005. Beternak Puyuh Secara K omersial.
Penebar Swadaya, J akarta.
Maenz, D. D. 2001. Enzymatic characteristic of phytases as they relate to their use
in animal feeds. In: Enzyme in A nimal Nutrition. (Ed. M. R. Bedford and
G. G. Partridge). CA BI Pub. United K ingdom. 3: 61-84.
Maia G.A .R., J .B. Fonseca, R.T .R.N. Soares, M.A . Silva and C.L .M. Souza. 2002.
Egg quality of laying hens fed with dry yeast sugarcane. Brazilian
A gricultural Research. 37: 1295-1300.
Marsudi dan C. Saparinto. 2012. Puyuh. Penebar Swadaya, J akarta.
Muhilal dan A . Sulaiman. 2004. A ngka kecukupan vitamin larut lemak. J akarta.
L embaga Ilmu Pengetahuan Indonesia. 356.
Nascimento G.R., A . E. Murakami, A . F. Q. M. Guerra, I. C. Ospinas-Rojas, M.
F. Z. Ferreira and J . C. Fanhani. 2014. Effect of different vitamin d sources
and calcium levels in the diet of layers in the second laying cycle.
Brazilian J ournal of Poultry Science. 16(2): 37-42.
Nesheim, M. C., R. E. A ustic and L . E. Card. 1979. Poultry Production. L ea
Febiger, Philadelphia.
Nuhriawangsa, A . M. P. 2012. Produksi Serbuk Fitase Hasil Teknologi
Rekombinan dan A plikasinya Untuk Meningkatkan K ualitas Pakan dan
K inerja A yam Broiler. Disertasi. Program Pascasarjana Fakultas
Peternakan. Universitas Gadjah Mada. Y ogyakarta.
Nutrition Research Council. 1994. Nutrient Requirement of Poultry. National
A cademy Science. Washington. United State of A merica.
Pedrosa, M.A .C. and M.L . Castro. 2005. Role of vitamin d in the neuro-muscular
function brazilian archives of endocrinology and metabolism. 49: 495-502.
R Core Team. 2015. R: A language and environment for statistical computing. R
Foundation for Statistical Computing. V ienna, A ustria. URL.
http://www.R-Project.org/.
Selle, P. H.,V . Ravindran, A .J . Cowieson and M. R. Bedford. 2010. Phytate and
phytase. In: enzymes in farm animal nutrition (Ed. M. Bedford and G.
Partridge). CA BI Publishing, Wallingford, Oxon, UK . pp. 167.
Shanaway, M. M. 1994. Quail Production Systems: A Review. Food and
A griculture Organization of the United Nations, Rome. Italy.
25
Shin, S., N. C. Ha, B. C. Oh, T. K . Oh and B.H. Oh. 2001. Enzyme mechanism
and catalytic property of propeller phytase. Structure. 9: 851-858.
Situmorang, N. A ., L .D. Mahfudz dan U. A tmomarsono. 2013. The influence of
seaweed (Gracilaria verrucosa) meal in the diet on protein utilization of
broiler. A nimal A gricultural J ournal. 2: 49-56.
Soares, J .H., J .M. K err and R.W. Gray. 1995. 25-hydroxycholecalciferol in
poultry nutrition. Poultry Science. 74: 1919-1934.
Standar Nasional Indonesia. 2006. Pakan A nak A yam Ras Pedaging (Broiler
Starter). Badan Standardisasi Nasional, J akarta.
Steel. R.G. D dan J . H. Torrie. 1993. Prinsip dan Prosedur Statistika Suatu
Pendekatan Biometrik. Terjemahan: M. Syah. PT. Gramedia Pustaka
Utama. J akarta.
Suprapto, W. S. K ismiyati dan E. Suprijatna. 2012. Pengaruh penggunaan tepung
kerabang telur ayam ras dalam pakan burung puyuh terhadap tulang tibia
dan tarsus. A nimal A gricultural J ournal. 1: 77-85.
Taylor, T.G. 2006. Calcium metabolism and the laying hen. J ournal of the
Science of Food and A griculture. 14: 611-613.
Tsang, C. P. W. and A . A . Grunder. 1993. Effect of dietary contents of
25-dihydroxycholecalciferol and 24, 25cholecalciferol,
dihydroxycholecalciferol
on
blood
concentrations
of
2525-dihydroxycholecalciferol, total calcium and
eggshell quality. British Poultry Science. 34: 1021-1027.
Umar. 2000. K ualitas Fisik Telur A yam K ampung Segar di Pasar Tradisional,
Swalayan dan Peternak di kota Madya Bogor. Fakultas Peternakan, Institut
Pertanian Bogor.
Underwood, E.J . and N.F. Suttle. 2001. The Mineral Nutrition of L ivestock. 3rd
Ed. CA BI Publishing, Wallingford, Oxon, UK . pp.65-104.
Winarno, F.G. 2004. K imia Pangan dan Gizi. PT Gramedia Pustaka Utama,
J akarta.
Wulandari, E. C., W. Murningsih dan H. I. Wahyuni. 2012. Deposisi kalsium dan
phosphor pada cangkang telur ayam arab dengan pemberian berbagai level
Azolla microphylla. A nimal A griculture J ournal. 1: 507-520.
Y uwanta, T. 2004. Dasar Ternak Unggas. K anisius, Y ogyakarta.
26
L A MPIR A N
27
L ampiran 1. Hasil A nalisis V ariansi K onsumsi K alsium
Bartlett test of homogeneity of variances
Data: konsumsi Ca by Perlakuan
Bartlett's K -squared = 1.1465, df = 4, p-value = 0.8868
Df Sum Sq Mean Sq F value Pr(>F)
prlk
4 0.00668 0.001670 0.599 0.667
Residuals 20 0.05572 0.002786
Histogram K onsumsi C a
28
L ampiran 2. Hasil A nalisis V ariansi Bobot K erabang
Bartlett test of homogeneity of variances
data: Bobot K erabang by perlakuan
-squared = 1.1465, df = 4, p-value = 0.8868
Df
Sum Sq
Mean Sq
F value
perlakaun
4
0.04544
0.011360
6.353
Residuals
20
0.03576
0.001788
Mean Square Error: 0.001788
perlakuan, means
bk
std
r
Min
Max
P0
0.640
0.02121320
5
0.62
0.67
P1
0.750
0.03741657
5
0.71
0.79
P2
0.642
0.07328028
5
0.56
0.74
P3
0.698
0.02489980
5
0.66
0.72
P4
0.650
0.03316625
5
0.61
0.68
29
Pr(>F)
0.00181 **
alpha: 0.05 ; Df Error: 20
Critical Range
2
0.05578538
3
4
5
0.05855591
0.06031664
0.06154641
Means with the same letter are not significantly different.
Groups, Treatments and means
a
P1
0.75
ab
P3
0.698
b
P4
0.65
b
P2
0.642
b
P0
0.64
30
L ampiran 3. Hasil A nalisis V ariansi Massa K alsium K erabang
Bartlett test of homogeneity of variances
data: massa Ca kerabang by perlakuan
Bartlett's K -squared = 7.5875, df = 4, p-value = 0.1079
Df
Sum Sq
Mean Sq
F value
prlk
4
0.005856
0.001464
6.595
Residuals
20
0.004440
0.000222
Histogram Massa C a K erabang
31
Pr(>F)
0.00149 **
Duncan's new multiple range test for mcakb
Mean Square Error: 0.000222
prlk, means
mcakb
std
r
Min
Max
P0
0.234
0.005477226
5
0.23
0.24
P1
0.266
0.018165902
5
0.25
0.29
P2
0.222
0.022803509
5
0.20
0.25
P3
0.246
0.011401754
5
0.23
0.26
P4
0.230
0.010000000
5
0.22
0.24
alpha: 0.05 ; Df Error: 20
Critical Range
2
0.01965682
3
4
5
0.02063305
0.02125347
0.02168680
Means with the same letter are not significantly different.
Groups, Treatments and means
a
P1
0.266
b
P3
0.246
bc
P0
0.234
bc
P4
0.23
c
P2
0.222
32
L ampiran 4. Hasil A nalisis V ariansi Pemanfaatan K alsium
Bartlett test of homogeneity of variances
data: pemanfaatan Ca by perlakuan
Bartlett's K -squared = 2.6633, df = 4, p-value = 0.6156
Df
Sum Sq
Mean Sq
F value
Pr(>F)
prlk
4
48.37
12.093
2.045
0.127
Residuals
20
118.28
5.914
Histogram Pemanfaatan C a
33
L ampiran 6. Ucapan T erima K asih
UC A PA N T E R IMA K A SIH
Skripsi berjudul Pemanfaatan K alsium Pada Puyuh (Coturnix Coturnix
J aponica) Y ang Mendapat Suplementasi V itamin D Dalam R ansum Y ang
Mengandung F itase merupakan bagian penelitian dari :
Nama Dosen
: Dr. A di Magna P. N., S.Pt., M.P
Dr.sc.agr. A di Ratriyanto, S.Pt., M.P
Rysca Indreswari, S.Pt., M.Si
Winny Swastike, S.Pt., M.P
J udul Penelitian
:
A plikasi fitase untuk meningkatkan kualitas pakan dan
Produksi
puyuh
petelur
dengan
limbah
ramah
lingkungan
Skema Penelitian
: Hibah Penelitian Strategis Nasional
Tahun
: 2015
Sumber Dana
: DP2M DIK T I
Nomor K ontrak
: 339/UN27.11/PL/2015
Penulis mengucapkan terima kasih atas kesempatan yang diberikan untuk
melaksanakan penelitian tersebut.
Surakarta,
Mei 2016
Penulis
Eprilia A ristia Rini
34
Suplementasi vitamin D dalam ransum yang mengandung fitase 500 FTU,
Ca 3,3%, P tersedia 0,5% pada level 500 IU dapat meningkatkan bobot dan massa
Ca kerabang.
22
DA F T A R PUST A K A
A bdel-A zeem, F. A . and A . A . El-Shafei. 2006. Effect of different levels of
cholecalciferol (vitamin D3) on performance and skeletal bone formation
of laying J apanese quail reared under hot climate region. Egyptian Poultry
Science J ournal. 26: 221-234.
A lmatsier, S. 2004. Prinsip Dasar Ilmu Gizi. PT. Gramedia Pustaka Utama,
J akarta.
A min, M., D. J usadi dan I. Mokoginta. 2011. Penggunaan enzim fitase untuk
meningkatkan ketersediaan fosfor dari sumber bahan nabati pakan dan
pertumbuhan ikan lele (Clarias sp). J urnal Saintek Perikanan. 6: 52-60.
A mrullah, I. K . 2003. Nutrisi A yam Petelur. L embaga Satu Gunungbudi, Bogor.
A nggorodi, H. R. 1995. Nutrisi A neka Ternak Unggas. PT Gramedia Pustaka,
J akarta.
A smarasari, S. A . dan E. Suprijatna. 2007. Respon pemberian pellet kunyit
(Curcuma domestica) terhadap performans produksi dan efisiensi
penggunaan protein ayam pedaging. Dalam: Prosiding Seminar Nasional
Hari Pangan Sedunia X X V II. Universitas Diponegoro. Semarang. hal.
251-256.
A stawan, M. 2009. Sehat dengan Hidangan K acang dan Biji-bijian. Penebar
Swadaya, J akarta.
A ureli, R., M. U. Faruk, I. Cechova, P. B. Pedersen, S.G. Elvig-J oergensen, F. Fru
and J . Broz. 2011. The efficacy of a novel microbial 6-phytase expressed
in Aspergillus oryzae on the performance and phosphorus utilization in
broiler chickens. International J ournal of Poultry Science. 10: 160-168.
Baruah, K ., N.P. Sahu, A .K . Pal and D. Debnath . 2004. Dietary phytase: an ideal
approach for a cost effective and low-polluting aqua feed. NA GA , World
Fish Center Quarterly. 27: 15-19.
Blakely, J . dan D. H. Bade. 1998. Ilmu Peternakan. Edisi ke Empat.
(Diterjemahkan oleh: B. Srigandono). Gadjah Mada University Press.
Y ogyakarta.
Bradfield, J . R. G. 1951. Radiographic studies on the formation of the hen's egg
shell. J ournal of Experimental Biology. 28: 125-141.
Costa, C.H.R., S.L.T. Barreto, P.C. Gomes, L .H. Hosoda, C.A . L ipari and H.J .D.
L ima. 2011. A vailable phosphorus levels in diets for J apanese quail 45-57
weeks of age. J ournal of A nimal Science. 40: 2152-2160.
Clunies, M., D. Parks and S. L eeson. 1992. Calcium and phosporus metabolism
and eggshell formation of hens fed different amounts of calcium. Poultry
Science. 71: 482-489.
23
Edwards, H. M., J r. 1993. Dietary 1,25-dihydroxycholecalciferol supplementation
increases natural phytate phosphorus utilization in chickens. J ournal of
Nutrition. 123: 567-577.
Edwards, H. M., J r., M. A . Elliot and S. Sooncharernying. 1992. Effect of dietary
calcium on tibial dyschondroplasia. Interaction with light, cholecalciferol,
1,25-dihydroxycholecalciferol, protein, and synthetic zeolite. Poultry
Science. 71: 2041-2055.
Eklund, M., R. Mosenthin, M. Tafaj and J . Wamatu. 2006. Effects of betaine and
condensed molasses solubles on nitrogen balance and nutrient digestibility
in piglets fed diets deficient in methionine and low in compatible
osmolytes. A rchieves of A nimal Nutrition. 60: 289-300.
El-Maksoud, A . A . 2010. Effect of dietary calcium and vitamin D3 levels on egg
production and egg shell quality of hy-line brown - egg type laying hens.
Egyptian Poultry Science J ournal. 30: 1097-1120.
Etches, R. J . 1996. Reproduction in Poultry. CA B International. Wallingford.
Oxon. UK .
Ferket, P. R and A . G. Gernat. 2006. Factors that affect feed intake of meat birds:
A Review. International J ournal of Poultry Science. 5: 905-911.
Frost and Roland. 1990. Influence of vitamin D3, 1 alpha-hydroxyvitamin D3, and
1,25-dihydroxyvitamin D3 on eggshell quality, tibia strength, and various
production parameters in commercial laying hens. Poultry Science. 69:
2008-2016.
Garcia A . F. Q. M., E. M. A lice, R. A . D. Cristiane, C. O. R. Iván, K arla Paola
Picoli and M. Z. Maíra. 2013. Use of vitamin D and its metabolites in
broiler chicken feed on performance, bone parameters and meat quality.
A sian-A ustralasian J ournal of A nimal Science. 26(3): 408-415
Greiner, R., E. Haller, U. K onietzny and K .D. J any. 1997. Purification and
characterization of a phytase from Klebsiella terrigena. A rchieves
Biochemistry Biophysics. 341: 201-206.
Hartadi, H., S. Reksohadiprojo dan A . D. Tillman. 1994. T abel K omposisi Pakan
untuk Indonesia. Gadjah Mada University Press, Y ogyakarta.
Indreswari, R., H. I. Wahyuni, N. Suthama and P. W. Ristiana. 2009. Calcium
utilization for egg shell formation in laying hens due to the different
feeding regimes of morning and afternoon portion. J ournal of the
Indonesian Tropical A nimal A griculture. 34: 134-138.
K asiyati, N. K usumorini, H. Maheshwari dan W. Manalu. 2010. K ajian fisiologis
status kalsium puyuh (Coturnix coturnix japonica) setelah pemberian
cahaya monokromatik. Buletin A natomi dan Fisiologi. 18: 1-11.
K eshavarz, K . 2003. A comparison between cholecalciferol and 25-OHcholecalciferol on performance and eggshell quality of hens fed different
levels of calcium and phosporus. Poultry Science. 82: 1415-22.
24
L avelin, I., N. Meiri and M. Pines. 2000. New insight in eggshell formation.
Poultry Science. 79: 1014-1017.
L eeson, S and J . D. Summer. 2001. Feeding Programs for L aying Hens. Buletin
A SA Technical V olume PO49. A merican Soybean A ssociation.
L istiyowati, E dan K . Roospitasari. 2005. Beternak Puyuh Secara K omersial.
Penebar Swadaya, J akarta.
Maenz, D. D. 2001. Enzymatic characteristic of phytases as they relate to their use
in animal feeds. In: Enzyme in A nimal Nutrition. (Ed. M. R. Bedford and
G. G. Partridge). CA BI Pub. United K ingdom. 3: 61-84.
Maia G.A .R., J .B. Fonseca, R.T .R.N. Soares, M.A . Silva and C.L .M. Souza. 2002.
Egg quality of laying hens fed with dry yeast sugarcane. Brazilian
A gricultural Research. 37: 1295-1300.
Marsudi dan C. Saparinto. 2012. Puyuh. Penebar Swadaya, J akarta.
Muhilal dan A . Sulaiman. 2004. A ngka kecukupan vitamin larut lemak. J akarta.
L embaga Ilmu Pengetahuan Indonesia. 356.
Nascimento G.R., A . E. Murakami, A . F. Q. M. Guerra, I. C. Ospinas-Rojas, M.
F. Z. Ferreira and J . C. Fanhani. 2014. Effect of different vitamin d sources
and calcium levels in the diet of layers in the second laying cycle.
Brazilian J ournal of Poultry Science. 16(2): 37-42.
Nesheim, M. C., R. E. A ustic and L . E. Card. 1979. Poultry Production. L ea
Febiger, Philadelphia.
Nuhriawangsa, A . M. P. 2012. Produksi Serbuk Fitase Hasil Teknologi
Rekombinan dan A plikasinya Untuk Meningkatkan K ualitas Pakan dan
K inerja A yam Broiler. Disertasi. Program Pascasarjana Fakultas
Peternakan. Universitas Gadjah Mada. Y ogyakarta.
Nutrition Research Council. 1994. Nutrient Requirement of Poultry. National
A cademy Science. Washington. United State of A merica.
Pedrosa, M.A .C. and M.L . Castro. 2005. Role of vitamin d in the neuro-muscular
function brazilian archives of endocrinology and metabolism. 49: 495-502.
R Core Team. 2015. R: A language and environment for statistical computing. R
Foundation for Statistical Computing. V ienna, A ustria. URL.
http://www.R-Project.org/.
Selle, P. H.,V . Ravindran, A .J . Cowieson and M. R. Bedford. 2010. Phytate and
phytase. In: enzymes in farm animal nutrition (Ed. M. Bedford and G.
Partridge). CA BI Publishing, Wallingford, Oxon, UK . pp. 167.
Shanaway, M. M. 1994. Quail Production Systems: A Review. Food and
A griculture Organization of the United Nations, Rome. Italy.
25
Shin, S., N. C. Ha, B. C. Oh, T. K . Oh and B.H. Oh. 2001. Enzyme mechanism
and catalytic property of propeller phytase. Structure. 9: 851-858.
Situmorang, N. A ., L .D. Mahfudz dan U. A tmomarsono. 2013. The influence of
seaweed (Gracilaria verrucosa) meal in the diet on protein utilization of
broiler. A nimal A gricultural J ournal. 2: 49-56.
Soares, J .H., J .M. K err and R.W. Gray. 1995. 25-hydroxycholecalciferol in
poultry nutrition. Poultry Science. 74: 1919-1934.
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L A MPIR A N
27
L ampiran 1. Hasil A nalisis V ariansi K onsumsi K alsium
Bartlett test of homogeneity of variances
Data: konsumsi Ca by Perlakuan
Bartlett's K -squared = 1.1465, df = 4, p-value = 0.8868
Df Sum Sq Mean Sq F value Pr(>F)
prlk
4 0.00668 0.001670 0.599 0.667
Residuals 20 0.05572 0.002786
Histogram K onsumsi C a
28
L ampiran 2. Hasil A nalisis V ariansi Bobot K erabang
Bartlett test of homogeneity of variances
data: Bobot K erabang by perlakuan
-squared = 1.1465, df = 4, p-value = 0.8868
Df
Sum Sq
Mean Sq
F value
perlakaun
4
0.04544
0.011360
6.353
Residuals
20
0.03576
0.001788
Mean Square Error: 0.001788
perlakuan, means
bk
std
r
Min
Max
P0
0.640
0.02121320
5
0.62
0.67
P1
0.750
0.03741657
5
0.71
0.79
P2
0.642
0.07328028
5
0.56
0.74
P3
0.698
0.02489980
5
0.66
0.72
P4
0.650
0.03316625
5
0.61
0.68
29
Pr(>F)
0.00181 **
alpha: 0.05 ; Df Error: 20
Critical Range
2
0.05578538
3
4
5
0.05855591
0.06031664
0.06154641
Means with the same letter are not significantly different.
Groups, Treatments and means
a
P1
0.75
ab
P3
0.698
b
P4
0.65
b
P2
0.642
b
P0
0.64
30
L ampiran 3. Hasil A nalisis V ariansi Massa K alsium K erabang
Bartlett test of homogeneity of variances
data: massa Ca kerabang by perlakuan
Bartlett's K -squared = 7.5875, df = 4, p-value = 0.1079
Df
Sum Sq
Mean Sq
F value
prlk
4
0.005856
0.001464
6.595
Residuals
20
0.004440
0.000222
Histogram Massa C a K erabang
31
Pr(>F)
0.00149 **
Duncan's new multiple range test for mcakb
Mean Square Error: 0.000222
prlk, means
mcakb
std
r
Min
Max
P0
0.234
0.005477226
5
0.23
0.24
P1
0.266
0.018165902
5
0.25
0.29
P2
0.222
0.022803509
5
0.20
0.25
P3
0.246
0.011401754
5
0.23
0.26
P4
0.230
0.010000000
5
0.22
0.24
alpha: 0.05 ; Df Error: 20
Critical Range
2
0.01965682
3
4
5
0.02063305
0.02125347
0.02168680
Means with the same letter are not significantly different.
Groups, Treatments and means
a
P1
0.266
b
P3
0.246
bc
P0
0.234
bc
P4
0.23
c
P2
0.222
32
L ampiran 4. Hasil A nalisis V ariansi Pemanfaatan K alsium
Bartlett test of homogeneity of variances
data: pemanfaatan Ca by perlakuan
Bartlett's K -squared = 2.6633, df = 4, p-value = 0.6156
Df
Sum Sq
Mean Sq
F value
Pr(>F)
prlk
4
48.37
12.093
2.045
0.127
Residuals
20
118.28
5.914
Histogram Pemanfaatan C a
33
L ampiran 6. Ucapan T erima K asih
UC A PA N T E R IMA K A SIH
Skripsi berjudul Pemanfaatan K alsium Pada Puyuh (Coturnix Coturnix
J aponica) Y ang Mendapat Suplementasi V itamin D Dalam R ansum Y ang
Mengandung F itase merupakan bagian penelitian dari :
Nama Dosen
: Dr. A di Magna P. N., S.Pt., M.P
Dr.sc.agr. A di Ratriyanto, S.Pt., M.P
Rysca Indreswari, S.Pt., M.Si
Winny Swastike, S.Pt., M.P
J udul Penelitian
:
A plikasi fitase untuk meningkatkan kualitas pakan dan
Produksi
puyuh
petelur
dengan
limbah
ramah
lingkungan
Skema Penelitian
: Hibah Penelitian Strategis Nasional
Tahun
: 2015
Sumber Dana
: DP2M DIK T I
Nomor K ontrak
: 339/UN27.11/PL/2015
Penulis mengucapkan terima kasih atas kesempatan yang diberikan untuk
melaksanakan penelitian tersebut.
Surakarta,
Mei 2016
Penulis
Eprilia A ristia Rini
34