Effect Of Sprouts (Phaseolus Aureus) Addition In Ration On The Quality Of Friesian Bull Semen Repository - UNAIR REPOSITORY
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Volumul a fost editat cu sprijinul financiar
al Ministerului Educa~ie
Na~ionle
THE ORGANIZING COMMITTEES OF THE ANNUAL SCIENTIFIC
CONFERENCE ,MODERN ANIMAL HUSBANDRY- STRATEGIES,
2013
OPPORTUNITIES AND PERFORMANCE" IA~,
:OMITETUL DE ORGANIZARE:
EXECUTIVE COMMITTEE):
lembri:
CD-ROM
Prof.dr. Benone PASARIN - decan,
Facultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
Prof.dr. Constantin PASCAL- prodecan ~tinfc,
Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof. dr. loan GiLCA - prodecan,
Facultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
Prof.dr. Paul Corneliu BOI~TEANU
- prorector,
U.S.A.M. V. la§i, Romania
Prof.dr.loan VACARU OPRI~Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof.dr. ~teofil
CREANGA - director departament,
Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof.dr. Marius Giorgi USTUROI director departament, Facultatea de Zootehnie,
U.S.A. M. V. la§i, Romania
Prof.dr. loan Mircea POP Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Conf.dr. Vasile MACIUC Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
~ef
lucr.dr. Mihaela IVANCIAFacultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
~ef
lucr.dr. Gabriel HOHAFacultatea de Zootehnie, U.S.A.M. V. la§i, Romania
ISSN
2284 - 6964
ISSN-L 1454 -7368
© Editura "lon lonescu de Ia Brad"
la~i
COMITETUL EDITORIAL:
(EDITORIAL BOARD)
Redactor responsabil:
Prof. dr. Vasile ViNTU U.S.A.M.V. la§i, Romania
Prof. dr. loan TENU U.S.A.M.V. la§i, Romania
Prof. dr. Teodor ROBU U.S.A.M.V. la§i, Romania
Prof. dr. Lucia DRAGHIA U.S.A.M.V. la§i, Romania
Prof. dr. Benone PASARIN U.S.A.M.V. la§i, Romania
Prof. dr. Liviu MIRON U.S.A.M.V. la§i, Romania
Redactor adjunct:
Membri:
COMITETUL ~TINFC:
(SCIENTIFIC COMMITTEE)
Prof.dr.ing. Benone PASARIN- Decan, Facultatea de Zootehnie,
U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Constantin PASCAL- Prodecan !jtiintific, Facultatea de
Zootehnie, U.S.A.M.V. la!ji, Romania
Prof.dr. Edi PIASENTIER - Universitatea Udine, ltalia
Prof.dr. dr. H.C. Liviu AI. MARGHIT~U.S.A.M.V. Cluj-Napoca, Romania
Prof.dr. Walter BAUMGARTNER- University of Veterinary Medicine Vienna,
·
Austria
Prof.dr. Diego BEGALLI - University of Verona, ltalia
Dr. H.C. Yves NYS - t.:institut National de Ia Rechearche Agronomique {INRA),
Director Research, Franta
Prof.dr. Otto SZENCI - Szent Istvan University, Budapest - Ungaria
Prof.dr. Peter VOS - Utrecht University, Olanda
Prof.dr. Mark RUSSELL - College of Agriculture, Purdue University, Indiana, USA
Prof.dr. Fran~oise
PICARD - Universite d'Angers, Franta
Prof.dr. Mohamed A. KENAWI - Minia University, Egipt
Dr. Hendronoto A.W. LENGKEY- Padjadjaran University, lndonezia
Prof.dr.ing. loan VACRU-OPI~
U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Paul Corneliu BOI~TEANUU.S.A.M.V. la!ji, Romania
Prof.dr.ing. ~tefan
LAZAR- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. loan GiLCA- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. loan Mircea POP- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Marius Giorgi USTUROI- U.S.A.M.V. la!ji, Romania
Conf.dr.ing. Vasile MACIUC- U.S.A.M.V. la!ji, Romania
This volume includes scientific papers of the International Symposium
"Modern Animal Husbandry - Strategies, Opportunities and Peiformance"
25-26.04.2013, U.S.A.M V. Imji, ROMANIA
----------
Lucrari !?tiintifice-Seria Zootehnie, 2013
EFFECT OF SPROUTS (PHASEOLUS AUREUS) ADDITION
IN RATION ON THE QUALITY OF FRIESIAN BULL SEMEN
Soeparna Soeparna\ Widyawati Lina2, Sukraeni Eni\
Maidaswar Maidaswar2, Lestari Tita Damayanti1
e-mail: titadlestari@unpad.ac.id
Abstract
A research on effect of bean sprouts (Phaseolus aureus) addition in ration of Friesian bull has
been conducted in term to learn its effect towards motility and abnormality of their sperm. This
research was conducted at Artificial Insemination Center in Lembang District, West Java, using 16
head of Friesian bulls of 3 - 3.5 years old and 800- 900 kg of body weight. Treatment of ration
were TO: basal ration without bean sprout; T0.5: TO+ 0.5% bean sprout; T1.0: TO+ 1.0% bean
sprout; T1.5: TO+ 1.5% bean sprout. Feeding treatment was applied in two months and semen
collection was conducted weekly for four times started from the end offeeding treatment. The study
uses Completely Random Design. Each treatment was replicated 4 times. Data of this research
was analyzed by ANOVA. Conclusion of the research is that addition of 0.5% bean sprout on bull
ration is the optimum proportion to improve sperm motility and decrease sperm abnormality;
however, addition of bean sprout has no effect on the other variables of Friesian bull semen
qualities- either macroscopically or microscopically. And so, on the post thaw semen qualities that
measured via post thaw motility and conception rate.
Key words: Friesian Bull, Bean sprout, sperm quality
INTRODUCTION
The
successful
application
of
reproductive biotechnology in the form of
artificial insemination with frozen semen is
influenced by several factors, both biological
factors and technical factors. Semen quality
is one factor that plays a role in the success
of artificial insemination technology. Good
quality of semen is produced by healthy
males and appropriate fed by good quality of
nutrition. Good nutrition is nutrition balanced
protein and calories. To obtain the proteincalorie nutrition it needs to make various
efforts such as by preparing rations of any
material
alternative
that
contains
carbohydrates, fats, proteins, vitamins, and
minerals. Ration of males can be improving
semen quality in addition to meeting the
needs of those living males.
Complete feed ingredients containing
vitamins, minerals and materials are believed
to improve the quality of semen. Previous
studies said Soya bean sprout (Phaseolus
aureus), which contains vitamin A, C, and E,
as well as the minerals sodium, magnesium,
zinc, calium, and ferrum, which supposedly
can increase the fertility of males (Surahman
and Darmajana, 2004). If the composition of
the nutrient content is added to the ration, it
can supplement rations. Vitamin C, vitamin
E, amino acids, zinc and copper which are
contained in the sprouts affect the durability
of the oxygen reaction and protect sperm
from the sperm membrane from peroxide free
radicals. Thus allowing the addition of bean
sprouts in the ration increased sperm quality,
which is characterized by increased motility,
sperm concentration, decreased sperm
abnormalities (Oliveira, eta!, 2004; Yazama,
et a!., 2006; Cayurt, and Achan, 2008;
Alonzo eta!., 2009). This research was aimed
to fmd out the effect of giving sprouts in the
ration of FRESIEN bovine male semen
quality.
1
Universitas Padjadjaran, Sumedang West Java, Indonesia
Lembang AI Center. Jl. Kayu Ambon. Lembang, Indonesia
2
- 103-
University of Agricultural Sciences and Veterinary Medicine lasi
MATERIAL AND METHOD
This research was conducted at Artificial
Insemination Center in Lembang District,
West Java, using 16 head of Friesian bull of3
- 3.5 years old and 800 - 900 kg of body
weight. Commencing the research, the bulls
were given basal ration for 14 days for
adaptation. Feed was given twice daily, at 7
am and 2 pm. Treatment of ration were TO:
basal
ration
without
bean
sprout;
T0.5:T0+0.5% bean sprout; Tl.O:TO+ 1.0%
bean sprout; Tl.5:TO+ 1.5% bean sprout.
Research was conducted for 90 days.
Collecting semen was done once a week. It
started at day 59, then followed at day 73, 80
and 87 and these were as replications to value
the response of treatments.
Data analysis was Completely Random
Design, consists of four ration treatments to
the 16 bulls, as replication was four semen
collecting periods (day 59, 73, 80 and 87).
Parameters were semen volume, pH, sperm
concentration, sperm abnormality of fresh
semen and the quality after frozen.
RESULTS AND DISCUSSIONS
Effect of treatments on fresh semen is
shown at Table 1. Table 1 contains the
characteristic of Friesian bull fresh semen of
four treatments (TO; T0.5; Tl.O; Tl.5). Table
1 shows that the addition of bean sprout in
ration increase the number of progressively
motile sperm and decrease abnormalities
(P
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·~
,. ... ..:_
;,.
1M
li II:
'J '
'
..
,~.
}·~
,.' '
;
.. '
.
'
·'
...
'
'
..
'
I~\
,·~
:-"fl
' '
'•'
-
. ,_.
'~
r. "
~-,
'-'
<
~.
''
.. r.~_
t, ••
._
'c
'-
Volumul a fost editat cu sprijinul financiar
al Ministerului Educa~ie
Na~ionle
THE ORGANIZING COMMITTEES OF THE ANNUAL SCIENTIFIC
CONFERENCE ,MODERN ANIMAL HUSBANDRY- STRATEGIES,
2013
OPPORTUNITIES AND PERFORMANCE" IA~,
:OMITETUL DE ORGANIZARE:
EXECUTIVE COMMITTEE):
lembri:
CD-ROM
Prof.dr. Benone PASARIN - decan,
Facultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
Prof.dr. Constantin PASCAL- prodecan ~tinfc,
Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof. dr. loan GiLCA - prodecan,
Facultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
Prof.dr. Paul Corneliu BOI~TEANU
- prorector,
U.S.A.M. V. la§i, Romania
Prof.dr.loan VACARU OPRI~Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof.dr. ~teofil
CREANGA - director departament,
Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Prof.dr. Marius Giorgi USTUROI director departament, Facultatea de Zootehnie,
U.S.A. M. V. la§i, Romania
Prof.dr. loan Mircea POP Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
Conf.dr. Vasile MACIUC Facultatea de Zootehnie, U.S.A.M. V. la§i, Romania
~ef
lucr.dr. Mihaela IVANCIAFacultatea de Zootehnie, U.S.A.M. V. /a§i, Romania
~ef
lucr.dr. Gabriel HOHAFacultatea de Zootehnie, U.S.A.M. V. la§i, Romania
ISSN
2284 - 6964
ISSN-L 1454 -7368
© Editura "lon lonescu de Ia Brad"
la~i
COMITETUL EDITORIAL:
(EDITORIAL BOARD)
Redactor responsabil:
Prof. dr. Vasile ViNTU U.S.A.M.V. la§i, Romania
Prof. dr. loan TENU U.S.A.M.V. la§i, Romania
Prof. dr. Teodor ROBU U.S.A.M.V. la§i, Romania
Prof. dr. Lucia DRAGHIA U.S.A.M.V. la§i, Romania
Prof. dr. Benone PASARIN U.S.A.M.V. la§i, Romania
Prof. dr. Liviu MIRON U.S.A.M.V. la§i, Romania
Redactor adjunct:
Membri:
COMITETUL ~TINFC:
(SCIENTIFIC COMMITTEE)
Prof.dr.ing. Benone PASARIN- Decan, Facultatea de Zootehnie,
U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Constantin PASCAL- Prodecan !jtiintific, Facultatea de
Zootehnie, U.S.A.M.V. la!ji, Romania
Prof.dr. Edi PIASENTIER - Universitatea Udine, ltalia
Prof.dr. dr. H.C. Liviu AI. MARGHIT~U.S.A.M.V. Cluj-Napoca, Romania
Prof.dr. Walter BAUMGARTNER- University of Veterinary Medicine Vienna,
·
Austria
Prof.dr. Diego BEGALLI - University of Verona, ltalia
Dr. H.C. Yves NYS - t.:institut National de Ia Rechearche Agronomique {INRA),
Director Research, Franta
Prof.dr. Otto SZENCI - Szent Istvan University, Budapest - Ungaria
Prof.dr. Peter VOS - Utrecht University, Olanda
Prof.dr. Mark RUSSELL - College of Agriculture, Purdue University, Indiana, USA
Prof.dr. Fran~oise
PICARD - Universite d'Angers, Franta
Prof.dr. Mohamed A. KENAWI - Minia University, Egipt
Dr. Hendronoto A.W. LENGKEY- Padjadjaran University, lndonezia
Prof.dr.ing. loan VACRU-OPI~
U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Paul Corneliu BOI~TEANUU.S.A.M.V. la!ji, Romania
Prof.dr.ing. ~tefan
LAZAR- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. loan GiLCA- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. loan Mircea POP- U.S.A.M.V. la!ji, Romania
Prof.dr.ing. Marius Giorgi USTUROI- U.S.A.M.V. la!ji, Romania
Conf.dr.ing. Vasile MACIUC- U.S.A.M.V. la!ji, Romania
This volume includes scientific papers of the International Symposium
"Modern Animal Husbandry - Strategies, Opportunities and Peiformance"
25-26.04.2013, U.S.A.M V. Imji, ROMANIA
----------
Lucrari !?tiintifice-Seria Zootehnie, 2013
EFFECT OF SPROUTS (PHASEOLUS AUREUS) ADDITION
IN RATION ON THE QUALITY OF FRIESIAN BULL SEMEN
Soeparna Soeparna\ Widyawati Lina2, Sukraeni Eni\
Maidaswar Maidaswar2, Lestari Tita Damayanti1
e-mail: titadlestari@unpad.ac.id
Abstract
A research on effect of bean sprouts (Phaseolus aureus) addition in ration of Friesian bull has
been conducted in term to learn its effect towards motility and abnormality of their sperm. This
research was conducted at Artificial Insemination Center in Lembang District, West Java, using 16
head of Friesian bulls of 3 - 3.5 years old and 800- 900 kg of body weight. Treatment of ration
were TO: basal ration without bean sprout; T0.5: TO+ 0.5% bean sprout; T1.0: TO+ 1.0% bean
sprout; T1.5: TO+ 1.5% bean sprout. Feeding treatment was applied in two months and semen
collection was conducted weekly for four times started from the end offeeding treatment. The study
uses Completely Random Design. Each treatment was replicated 4 times. Data of this research
was analyzed by ANOVA. Conclusion of the research is that addition of 0.5% bean sprout on bull
ration is the optimum proportion to improve sperm motility and decrease sperm abnormality;
however, addition of bean sprout has no effect on the other variables of Friesian bull semen
qualities- either macroscopically or microscopically. And so, on the post thaw semen qualities that
measured via post thaw motility and conception rate.
Key words: Friesian Bull, Bean sprout, sperm quality
INTRODUCTION
The
successful
application
of
reproductive biotechnology in the form of
artificial insemination with frozen semen is
influenced by several factors, both biological
factors and technical factors. Semen quality
is one factor that plays a role in the success
of artificial insemination technology. Good
quality of semen is produced by healthy
males and appropriate fed by good quality of
nutrition. Good nutrition is nutrition balanced
protein and calories. To obtain the proteincalorie nutrition it needs to make various
efforts such as by preparing rations of any
material
alternative
that
contains
carbohydrates, fats, proteins, vitamins, and
minerals. Ration of males can be improving
semen quality in addition to meeting the
needs of those living males.
Complete feed ingredients containing
vitamins, minerals and materials are believed
to improve the quality of semen. Previous
studies said Soya bean sprout (Phaseolus
aureus), which contains vitamin A, C, and E,
as well as the minerals sodium, magnesium,
zinc, calium, and ferrum, which supposedly
can increase the fertility of males (Surahman
and Darmajana, 2004). If the composition of
the nutrient content is added to the ration, it
can supplement rations. Vitamin C, vitamin
E, amino acids, zinc and copper which are
contained in the sprouts affect the durability
of the oxygen reaction and protect sperm
from the sperm membrane from peroxide free
radicals. Thus allowing the addition of bean
sprouts in the ration increased sperm quality,
which is characterized by increased motility,
sperm concentration, decreased sperm
abnormalities (Oliveira, eta!, 2004; Yazama,
et a!., 2006; Cayurt, and Achan, 2008;
Alonzo eta!., 2009). This research was aimed
to fmd out the effect of giving sprouts in the
ration of FRESIEN bovine male semen
quality.
1
Universitas Padjadjaran, Sumedang West Java, Indonesia
Lembang AI Center. Jl. Kayu Ambon. Lembang, Indonesia
2
- 103-
University of Agricultural Sciences and Veterinary Medicine lasi
MATERIAL AND METHOD
This research was conducted at Artificial
Insemination Center in Lembang District,
West Java, using 16 head of Friesian bull of3
- 3.5 years old and 800 - 900 kg of body
weight. Commencing the research, the bulls
were given basal ration for 14 days for
adaptation. Feed was given twice daily, at 7
am and 2 pm. Treatment of ration were TO:
basal
ration
without
bean
sprout;
T0.5:T0+0.5% bean sprout; Tl.O:TO+ 1.0%
bean sprout; Tl.5:TO+ 1.5% bean sprout.
Research was conducted for 90 days.
Collecting semen was done once a week. It
started at day 59, then followed at day 73, 80
and 87 and these were as replications to value
the response of treatments.
Data analysis was Completely Random
Design, consists of four ration treatments to
the 16 bulls, as replication was four semen
collecting periods (day 59, 73, 80 and 87).
Parameters were semen volume, pH, sperm
concentration, sperm abnormality of fresh
semen and the quality after frozen.
RESULTS AND DISCUSSIONS
Effect of treatments on fresh semen is
shown at Table 1. Table 1 contains the
characteristic of Friesian bull fresh semen of
four treatments (TO; T0.5; Tl.O; Tl.5). Table
1 shows that the addition of bean sprout in
ration increase the number of progressively
motile sperm and decrease abnormalities
(P