1. Home
  2. Lainnya

Capsular Typing of Streptococcus pneumoniae Clinical Isolates In Bali.

 
 
 
 
 
 
 
 
 

 

Capsular Typing of Streptococcus pneumoniae Clinical Isolates
In Bali
Ni Nengah Dwi Fatmawati1,2*, Ni Made Adi Tarini1, I Putu Bayu Mayura1
1

Department of Clinical Microbiology, Medical School, Faculty of Medicine, Udayana University
2
Molecular Biology Laboratory, Faculty of Medicine, Udayana University, Bali, Indonesia
Jl. PB. Sudirman, Denpasar, Bali, Indonesia

*nnd.fatmawati@unud.ac.id

As a part of tropical area in Indonesia, Bali is easy to prone with infectious diseases including
respiratory tract infections. One of respiratory tract infectious agents is Streptococcus
pneumoniae. This gram-positive bacterium can cause not only respiratory tract infections
(pneumonia), but also more invasive infections, such as sepsis and meningitis. One of S.
pneumoniae major virulence factors is capsule. Polysaccharides capsule of S. pneumoniae are
known as pneumococcal vaccine component. Among 90 capsule types of S. pneumoniae that
have been investigated, several types have been found to be dominant in Indonesia such as
types 6, 23, 15, 33 and 12 in West Nusa Tenggara, type 7F in Jakarta, and types 6A/B dan
15B/C in Central Java. However, almost no data is reported in Bali about capsule genotype of
S. pneumoniae. Therefore, the aim of this study was to determine capsule types of S.
pneumoniae isolates in Clinical Microbiology Laboratory, Sanglah General Hospital,
Denpasar, Bali. Twenty-one isolates that were isolated from blood (11/52.4%), sputum
(5/23.8%), and other clinical specimens (5/23.8%) were included in this study. Identification
of S. pneumoniae was based on sensitivity of the isolates against optochin. All isolates were
subjected to PCR for capsular typing. The result showed that 7 isolates (66.7%) were type
19F, 2 isolates (9.5%) were positive for 23F and also for type 6A/B. Furthermore, there was 1
isolate (4.8%) for each type 7F and 15B/C. Total 8 isolates (38.1%) were nontypeable
isolates. This study showed the capsular types of S. pneumoniae clinical isolates in Bali, so

far is quite unique if compared to those in other places in Indonesia. Further study now is still
in progress to determine and characterize the nontypeable isolates.
Keywords: Streptococcus pneumoniae, capsular type, PCR, Pneumococcal vaccine

 

Dokumen yang terkait

Analysis of pulsotypes of salmonella typhi isolates and their clinical profiles in typhoid fever patients

0 3 11

Role of β−lactamase in the susceptibility of clinical isolates to β−lactam antibiotics

0 3 6

DETECTION OF STREPTOCOCCUS PNEUMONIAE IN THROAT SWABS OF ELEMENTARY SCHOOL TEACHERS AT JATINANGOR.

0 1 1

Clinical signs of Diabetes Mellitus In Balinese Cats in Bali Indonesia.

0 0 5

Detection Glutamate-Dehydrogenase Gene of Streptococcus suis Clinical Isolates in Sanglah Hospital Bali.

0 0 2

Detection Metallo-Beta-Lactamase Gene IMP-1 and IMP-2 of Pseudomonas aeruginosa Clinical Isolates In Sanglah Hospital Bali.

0 1 1

Multiplex PCR for Detection of Capsular Polysaccharides Types of Streptococcus pneumoniae Clinical Isolates in Bali.

0 0 1

Multiplex PCR for Detection of Capsular Polysaccharides Types of Streptococcus pneumoniae Clinical Isolates in Bali.

0 0 7

PENENTUAN TIPE KAPSUL ISOLAT Streptococcus pneumoniae PADA RUMAH SAKIT RUJUKAN TERSIER DI BALI.

0 0 2

Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients 8050432

1 5 13