PENGARUH EKSTRAK PHALERIA MACROCARPA TERHADAP INDEK MITOSIS SEL DAN PERTUMBUHAN DIAMETER KARSINOMA EPIDERMOID - Diponegoro University | Institutional Repository (UNDIP-IR)
76
LAMPIRAN 1
Gambar / foto sel mitosis pada jaringan karsinoma epidermoid (anak panah merah
dengan perbesaran 400x)
Kelompok kontrol
Kelompok P1
(2)
77
LAMPIRAN 2
Analisa Statistik Perubahan Diameter Tumor
Case Summaries
Selisih diameter tumor
3 1.1000 .26458 1.0000 .90 1.40
3 .4333 .25166 .4000 .20 .70
3 .4000 .10000 .4000 .30 .50
3 -.2667 .20817 -.2000 -.50 -.10
12 .4167 .53739 .4000 -.50 1.40
Kelompok K P1 P2 P3 Total
N Mean Std. Deviation Median Minimum Maximum
Case Summaries 3 3 1.3000 2.4000 1.47986 1.34536 .6000 2.0000 .30 1.30 3.00 3.90 3 3 .5667 1.0000 .55076 .78102 .3000 .6000 .20 .50 1.20 1.90 3 3 1.1000 1.5000 1.47986 1.57162 .4000 .8000 .10 .40 2.80 3.30 3 3 .9667 .7000 1.15902 .95394 .4000 .2000 .20 .10 2.30 1.80 12 12 .9833 1.4000 1.08362 1.22771 .4000 1.0500 .10 .10 3.00 3.90 N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum Kelompok K P1 P2 P3 Total Diameter tumor pre test
Diameter tumor post
(3)
78
NPar Tests Kruskal-Wallis Test
Tests of Normality
.314 3 . .893 3 .363
.219 3 . .987 3 .780
.175 3 . 1.000 3 1.000
.292 3 . .923 3 .463
Kelompok K P1 P2 P3 Selisih diameter tumor
Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk
Lilliefors Significance Correction a.
Test of Homogeneity of Variances
Selisih diameter tumor
1.129 3 8 .394
Levene
(4)
79
Mann Whitney Test
Test Statisticsb
.000 6.000 -1.964 .049
.100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Tumor diameter diference
Not corrected for ties. a.
Grouping Variable: Group b.
Test Statisticsb
.000 6.000 -1.964 .049
.100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Tumor diameter diference
Not corrected for ties. a.
Grouping Variable: Group b.
(5)
80
Test Statisticsb
.000 6.000 -1.964 .049
.100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Tumor diameter diference
Not corrected for ties. a.
Grouping Variable: Group b.
(6)
81
Test Statisticsb
.000 6.000 -1.964 .049
.100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Tumor diameter diference
Not corrected for ties. a.
Grouping Variable: Group b.
Test Statisticsb
.000 6.000 -1.964 .049
.100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Tumor diameter diference
Not corrected for ties. a.
Grouping Variable: Group b.
(7)
82
LAMPIRAN 3
Analisa Statistik Indek Mitosis
NPar Tests
Kruskal-Wallis Test
Case Summaries
Index mitosis
3 3.2000 .34641 3.4000 2.80 3.40
3 1.1333 .11547 1.2000 1.00 1.20
3 1.0667 .11547 1.0000 1.00 1.20
3 .6667 .11547 .6000 .60 .80
12 1.5167 1.04606 1.1000 .60 3.40
Kelompok K P1 P2 P3 Total
N Mean Std. Deviation Median Minimum Maximum
Tests of Normality
.385 3 . .750 3 .000
.385 3 . .750 3 .000
.385 3 . .750 3 .000
.385 3 . .750 3 .000
Kelompok K P1 P2 P3 Index mitosis
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova Shapiro-Wilk
Lilliefors Significance Correction a.
Test of Homogeneity of Variances
Index mitosis
5.333 3 8 .026
Levene
Statistic df1 df2 Sig.
Ranks 3 11.00 3 7.00 3 6.00 3 2.00 12 Kelompok K P1 P2 P3 Total Index mitosis
(8)
83
NPar Tests
Mann-Whitney Test
NPar Tests
Mann-Whitney Test
Test Statisticsa,b9.804 3 .020 Chi-Square df Asymp. Sig. Index mitosis
Kruskal Wallis Test a.
Grouping Variable: Kelompok b.
Ranks
3 5.00 15.00
3 2.00 6.00
6 Kelompok K P1 Total Index mitosis
N Mean Rank Sum of Ranks
Ranks
3 5.00 15.00
3 2.00 6.00
6 Kelompok K P2 Total Index mitosis
N Mean Rank Sum of Ranks
Test Statisticsb
.000 6.000 -2.023 .043 .100a Mann-Whitney U Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis
Not corrected for ties. a.
Grouping Variable: Kelompok b.
(9)
84
NPar Tests
Mann-Whitney Test
NPar Tests
Mann-Whitney Test
Ranks
3 5.00 15.00
3 2.00 6.00
6 Kelompok
K P3 Total Index mitosis
N Mean Rank Sum of Ranks
Test Statisticsb
.000 6.000 -2.023 .043 .100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis
Not corrected for ties. a.
Grouping Variable: Kelompok b.
Ranks
3 4.00 12.00
3 3.00 9.00
6 Kelompok
P1 P2 Total Index mitosis
(10)
85
NPar Tests
Mann-Whitney Test
Test Statisticsb3.000 9.000 -.745 .456 .700a Mann-Whitney U Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis
Not corrected for ties. a.
Grouping Variable: Kelompok b.
Ranks
3 5.00 15.00
3 2.00 6.00
6 Kelompok P1 P3 Total Index mitosis
N Mean Rank Sum of Ranks
Test Statisticsb
.000 6.000 -2.023 .043 .100a Mann-Whitney U Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis
Not corrected for ties. a.
Grouping Variable: Kelompok b.
(11)
86
NPar Tests
Mann-Whitney Test
Ranks
3 5.00 15.00
3 2.00 6.00
6 Kelompok
P2 P3 Total Index mitosis
N Mean Rank Sum of Ranks
Test Statisticsb
.000 6.000 -2.023 .043 .100a Mann-Whitney U
Wilcoxon W Z
Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis
Not corrected for ties. a.
Grouping Variable: Kelompok b.
(12)
87
LAMPIRAN 4
Uji Korelasi Selisih Diameter Tumor dan Indek Mitosis
Explore
Nonparametric Correlations
Case Summaries 12 12 .4167 1.5167 .53739 1.04606 .4000 1.1000 -.50 .60 1.40 3.40 N Mean Std. Deviation Median Minimum Maximum Selisih diametertumor Index mitosis
Tests of Normality
.105 12 .200* .988 12 .999
.369 12 .000 .742 12 .002
Selisih diameter tumor Index mitosis
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova Shapiro-W ilk
This is a lower bound of the true significance. *.
Lilliefors Significance Correction a. Correlations 1.000 .813** . .001 12 12 .813** 1.000 .001 . 12 12 Correlation Coefficient Sig. (2-tailed) N Correlation Coefficient Sig. (2-tailed) N
Selisih diameter tumor
Index mitosis Spearman's rho
Selisih diameter
tumor Index mitosis
Correlation is significant at the 0.01 level (2-tailed). **.
(13)
88
LAMPIRAN 5
Patologi Anatomi Sampel, Ukuran Diameter Tumor Pre dan Post Perlakuan, Indek
Mitosis Post Perlakuan
SCORING INDEX M ITOSIS DALAM 5 LAPANG
PANDANG BESAR HASIL PATOLOGI ANATOM I
KELOM POK I II III IV V M ean
Kont rol
A 4 3 3 5 2 3.4 SQUAM OUS CELL CA WELL
DIFFERENTIATED
B 2 3 4 3 2 2.8 SQUAM OUS CELL CA Insit u
C 4 3 2 3 5 3.4 SQUAM OUS CELL CA Insit u
m ean 3.2
P1
A 1 2 1 1 1 1.2
KERATOACANTOM A DENGAN SQUAM OUS CELL CA
M IKROINVASIF
B 0 1 2 1 1 1 PAPILOM A CELL SQUAM OUS
DENGAN FOKUS CA
C 3 1 1 1 0 1.2 SQUAM OUS CELL CA Insit u
M ean 3.4
P2
A 1 1 1 0 2 1 SQUAM OUS CELL CA WELL
DIFF DENGAN NEKROSIS LUAS
B 2 2 0 1 1 1.2 SQUAM OUS CELL CA WELL
DIFFERENTIATED
C 1 0 2 2 0 1 SQUAM OUS CELL CA WELL
DIFFERENTIATED
m ean 1.06
P3
A 0 1 1 2 0 0.8 SQUAM OUS CELL CA WELL
DIFFERENTIATED
B 1 0 0 1 1 0.6 SQUAM OUS CELL CA Insit u
C 1 1 0 1 0 0.6 SQUAM OUS CELL CA INSITU +
M IKROINVASIF
(14)
89
Data Ukuran Diameter Tumor Pre dan Post Perlakuan
Kelompok
Diameter
tumor pre test
(cm)
Diameter
tumor post
test (cm)
Selisih diameter
tumor (cm)
Kontrol
A
3
3,9
0,9
B
0,6
2
1,4
C
0,3
1,3
1
Mean
1,1
P1
A
1,2
1,9
0,7
B
0,2
0,6
0,4
C
0,3
0,5
0,2
Mean
0,43
P2
A
2,8
3,3
0,5
B
0,1
0,4
0,3
C
0,4
0,8
0,4
Mean
0,4
P3
A
2,3
1,8
-0,5
B
0,4
0,2
-0,2
C
0,2
0,1
-0,1
Mean
-0,26
Keterangan :
K
: Kontrol
P1
: Phaleria macrocarpa 0,0715 mg (0,36 ml/hari).
P2
: sitostatika Paclitaxel 175 mg/m
2dan cisplatin 50 mg/m
2P3
: sitostatika Paclitaxel 175 mg/m
2dan cisplatin 50 mg/m
2dan dikombinasikan
dengan Phaleria macrocarpa 0,0715 mg (0,36 ml/hari)
A
: tanda asam pikrat pada mencit di kepala
B
: tanda asam pikrat pada mencit di kaki depan
(15)
90
LAMPIRAN 6
Foto Mencit yang Diinduksi Tumor, Perlakuan, dan Pengukuran Tumor
Gambar. Mencit yang diinduksi
karsinoma epidermoid
Gambar. Pemberian Phaleria macrocarpa
via sonde
Gambar. Penyuntikan sitostatika
Gambar. Pengukuran diameter tumor
menggunakan caliper
(16)
91
LAMPIRAN 7
(17)
92
LAMPIRAN 8
(18)
93
LAMPIRAN 9
(19)
94
LAMPIRAN 10
(1)
89 Data Ukuran Diameter Tumor Pre dan Post Perlakuan
Kelompok
Diameter tumor pre test
(cm) Diameter tumor post test (cm) Selisih diameter tumor (cm) Kontrol
A 3 3,9 0,9
B 0,6 2 1,4
C 0,3 1,3 1
Mean 1,1
P1
A 1,2 1,9 0,7
B 0,2 0,6 0,4
C 0,3 0,5 0,2
Mean 0,43
P2
A 2,8 3,3 0,5
B 0,1 0,4 0,3
C 0,4 0,8 0,4
Mean 0,4
P3
A 2,3 1,8 -0,5
B 0,4 0,2 -0,2
C 0,2 0,1 -0,1
Mean -0,26
Keterangan :
K : Kontrol
P1 : Phaleria macrocarpa 0,0715 mg (0,36 ml/hari).
P2 : sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2
P3 : sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2 dan dikombinasikan dengan Phaleria macrocarpa 0,0715 mg (0,36 ml/hari)
A : tanda asam pikrat pada mencit di kepala B : tanda asam pikrat pada mencit di kaki depan C : tanda asam pikrat pada mencit di ekor
(2)
90 Foto Mencit yang Diinduksi Tumor, Perlakuan, dan Pengukuran Tumor
Gambar. Mencit yang diinduksi karsinoma epidermoid
Gambar. Pemberian Phaleria macrocarpa via sonde
Gambar. Penyuntikan sitostatika
Gambar. Pengukuran diameter tumor menggunakan caliper
(3)
91 LAMPIRAN 7
(4)
92 LAMPIRAN 8
(5)
93 LAMPIRAN 9
(6)